SSGCID: Target Page

LpinA.19760.a

Putative lipoprotein

CENTER ID:	LpinA.19760.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19760.a.B2.GE42332	mature protein		20	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.1015
UniProt:	Q72U43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQIFSIWLA ALLLFSGCAN ENGGNKSSSL LLSGIVSEQL LNIVADPKSS ESVIIIPTLY VDSISGSDSN AGTQSAPFKT ITKATLVATA SGIKVIAVAP GTYDASLGEV FPIYIPEGVN LYGDSNGKGL AGGSSSLYAG PPGTPPKTGP TLISGVGSFP PTIYFPTVVP ESSSQIAGFK INNPRFYDSG STVNAGIYLK NTGITVKNNT VTAVKGTGIS IHPSNGIGGG HVISGNFITS NSHGLHEADS NSNSKVEDNV ITQNWIGVST FDGLLDLGGG SRGSKGGNTL SCNTMYDLEV DVSQGFHFYA LNNFWDHIPL TIATFPDGSA TADLENSYQY AIMHISGSSV VSKPCNP

NT Sequence

atgaaacaaa tttttagtat ttggttggca gctttattgt tgttcagcgg atgtgccaac gaaaacggag gaaataaaag ttcttccctg ttgctttccg gaattgtcag cgaacagctg ttaaatatcg tggcggatcc aaaatcatcg gaatcggtca ttatcattcc tacgctttac gtagattcta tttccggaag cgattccaat gctggtacac aatccgctcc gtttaagacg attactaagg ccactttagt tgccacggcg agcggcatca aagtaattgc ggttgcaccg ggtacgtatg acgcgtccct gggagaagtt tttccaattt atattcccga aggtgtcaat ctatatgggg acagtaacgg gaaaggttta gcaggcggct cttcctccct ttatgcagga ccaccaggaa ctccacctaa gacaggacca actttgattt ctggagttgg aagttttccg ccgactattt attttcctac cgtggttcca gaatccagta gtcagatcgc agggtttaaa atcaataatc ctagatttta cgactctggt tctacagtta atgcgggaat ttatttaaaa aataccggaa ttacggtcaa aaataataca gtaactgcag taaaaggaac aggaattagc atccatcctt ctaatgggat cggaggggga cacgtcattt ccggtaattt cattacttcc aattctcacg gattgcatga agcggattca aattcgaata gcaaagtgga agacaatgtg atcactcaaa attggatcgg cgtttccact tttgatggtc ttcttgattt gggaggtgga agtcgtggaa gtaagggagg gaacactctt tcctgcaata caatgtatga cttggaagta gacgtttctc aagggtttca tttctatgct ttaaacaatt tctgggatca tatccccctt acgatcgcta catttccaga tggaagtgcg acggcggatc ttgaaaactc ctatcagtat gccattatgc atataagcgg aagtagtgtg gtttctaaac cttgtaatcc ttga

Details for LpinA.19760.a.B2.GE42332

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	36kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MNENGGNKSS SLLLSGIVSE QLLNIVADPK SSESVIIIPT LYVDSISGSD SNAGTQSAPF KTITKATLVA TASGIKVIAV APGTYDASLG EVFPIYIPEG VNLYGDSNGK GLAGGSSSLY AGPPGTPPKT GPTLISGVGS FPPTIYFPTV VPESSSQIAG FKINNPRFYD SGSTVNAGIY LKNTGITVKN NTVTAVKGTG ISIHPSNGIG GGHVISGNFI TSNSHGLHEA DSNSNSKVED NVITQNWIGV STFDGLLDLG GGSRGSKGGN TLSCNTMYDL EVDVSQGFHF YALNNFWDHI PLTIATFPDG SATADLENSY QYAIMHISG

Validated NT Sequence

ttccgcttat atgcataatg gcatactgat aggagttttc aagatccgcc gtcgcacttc catctggaaa tgtagcgatc gtaaggggga tatgatccca gaaattgttt aaagcataga aatgaaaccc ttgagaaacg tctacttcca agtcatacat tgtattgcag gaaagagtgt tccctccctt acttccacga cttccacctc ccaaatcaag aagaccatca aaagtggaaa cgccgatcca attttgagtg atcacattgt cttccacttt gctattcgaa tttgaatccg cttcatgcaa tccgtgagaa ttggaagtaa tgaaattacc ggaaatgacg tgtccccctc cgatcccatt agaaggatgg atgctaattc ctgttccttt tactgcagtt actgtattat ttttgaccgt aattccggta ttttttaaat aaattcccgc attaactgta gaaccagagt cgtaaaatct aggattattg attttaaacc ctgcgatctg actactggat tctggaacca cggtaggaaa ataaatagtc ggcggaaaac ttccaactcc agaaatcaaa gttggtcctg tcttaggtgg agttcctggt ggtcctgcat aaagggagga agagccgcct gctaaacctt tcccgttact gtccccatat agattgacac cttcgggaat ataaattgga aaaacttctc ccagggacgc gtcatacgta cccggtgcaa ccgcaattac tttgatgccg ctcgccgtgg caactaaagt ggccttagta atcgtcttaa acggagcgga ttgtgtacca gcattggaat cgcttccgga aatagaatct acgtaaagcg taggaatgat aatgaccgat tccgatgatt ttggatccgc cacgatattt aacagctgtt cgctgacaat tccggaaagc aacagggaag aacttttatt tcctccgttt tcgttcatat ggtggtggtg gt

Expected Protein Sequence

MAHHHHHHNE NGGNKSSSLL LSGIVSEQLL NIVADPKSSE SVIIIPTLYV DSISGSDSNA GTQSAPFKTI TKATLVATAS GIKVIAVAPG TYDASLGEVF PIYIPEGVNL YGDSNGKGLA GGSSSLYAGP PGTPPKTGPT LISGVGSFPP TIYFPTVVPE SSSQIAGFKI NNPRFYDSGS TVNAGIYLKN TGITVKNNTV TAVKGTGISI HPSNGIGGGH VISGNFITSN SHGLHEADSN SNSKVEDNVI TQNWIGVSTF DGLLDLGGGS RGSKGGNTLS CNTMYDLEVD VSQGFHFYAL NNFWDHIPLT IATFPDGSAT ADLENSYQYA IMHISGSSVV SKPCNP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacgaaa acggaggaaa taaaagttct tccctgttgc tttccggaat tgtcagcgaa cagctgttaa atatcgtggc ggatccaaaa tcatcggaat cggtcattat cattcctacg ctttacgtag attctatttc cggaagcgat tccaatgctg gtacacaatc cgctccgttt aagacgatta ctaaggccac tttagttgcc acggcgagcg gcatcaaagt aattgcggtt gcaccgggta cgtatgacgc gtccctggga gaagtttttc caatttatat tcccgaaggt gtcaatctat atggggacag taacgggaaa ggtttagcag gcggctcttc ctccctttat gcaggaccac caggaactcc acctaagaca ggaccaactt tgatttctgg agttggaagt tttccgccga ctatttattt tcctaccgtg gttccagaat ccagtagtca gatcgcaggg tttaaaatca ataatcctag attttacgac tctggttcta cagttaatgc gggaatttat ttaaaaaata ccggaattac ggtcaaaaat aatacagtaa ctgcagtaaa aggaacagga attagcatcc atccttctaa tgggatcgga gggggacacg tcatttccgg taatttcatt acttccaatt ctcacggatt gcatgaagcg gattcaaatt cgaatagcaa agtggaagac aatgtgatca ctcaaaattg gatcggcgtt tccacttttg atggtcttct tgatttggga ggtggaagtc gtggaagtaa gggagggaac actctttcct gcaatacaat gtatgacttg gaagtagacg tttctcaagg gtttcatttc tatgctttaa acaatttctg ggatcatatc ccccttacga tcgctacatt tccagatgga agtgcgacgg cggatcttga aaactcctat cagtatgcca ttatgcatat aagcggaagt agtgtggttt ctaaaccttg taatccttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at