LpinA.19722.a

RNA polymerase ECF-type sigma factor

CENTER ID: LpinA.19722.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19722.a.B1.GE41905 full length 1 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.257
UniProt: Q72VS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLSKDKTLD LVSRCGDGEE DALRQFFEIY SEDIYNFPMK IFHLSEDDAG DFFIYAFERL KTGSRFGSFK GKSSFRTWFY SVLRNMLIDW QRTKRELKVT NLGKISKEGK EYATIEDEPD TRPEMQEEAS EFSDRFNQAL EEIGVDKRVI FKLSYIYYLN LNEDEVQYLL EKTGLSPEAL KEKILHLRAE LSNREEENIR MEDKITSLYL NILELKEKQQ NTAKIAPILP MEVDKTSHAL KKKYEQRKRL LEKKKKGHFL ARTPYREVAE LIGISEGNVS VTLLRLIEKI QKKLDFSDLD F
NT Sequence
atgaatttgt caaaagataa aaccttagat ctcgtttctc gatgtggaga tggagaagaa gatgcgctga gacaattctt cgaaatctat tcggaagata tctacaattt cccaatgaaa atctttcatt tgagcgaaga cgacgctggg gattttttta tctatgcatt tgaaagatta aaaacaggct cccgttttgg aagttttaag ggaaaatcca gttttagaac ttggttctat tccgttctta gaaatatgct gattgattgg caaagaacca agcgagaact taaggttacc aatttaggaa aaattagtaa agaagggaag gaatacgcaa ccatagaaga cgaaccggat acacgtcctg aaatgcagga agaagccagt gaattttctg accgattcaa tcaggctctg gaagaaattg gggttgataa acgggtcatt tttaaacttt cttatattta ttatttaaac ttaaacgaag atgaagttca gtatcttctg gaaaaaacag gattatctcc tgaggcattg aaagagaaaa ttcttcatct cagagcggaa ctttccaatc gggaagaaga aaatattcga atggaagata aaattacctc tttatatcta aatatattag aattgaaaga aaaacagcaa aatactgcta aaatagctcc tattcttcct atggaagtgg ataaaacttc tcacgctttg aaaaagaaat atgagcagcg taaaagactt ttggaaaaga aaaagaaagg acacttttta gcaagaaccc cttacagaga agttgcagaa ttaattggga tttctgaggg gaacgtaagc gttactttac ttcgtttgat tgaaaaaatt cagaaaaagc tggatttcag tgatttggat ttttag
Details for LpinA.19722.a.B1.GE41905
HARVESTED ON: data unavailable
SEQUENCED ON: 9/29/2017
EXPECTED MW: 36kDa
OBSERVED MW: 6kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN LSKDKTLDLV SRCGDGEEDA LRQFFEIYSE DIYNFPMKIF HLSEDDAGDF FIYAFERLKT GSRFGSFKGK SSFRTWFYSV LRNMLIDWQR TKRELKVTNL GKISKEGKEY ATIEDEPDTR PEMQEEASEF SDRFNQALEE IGVDKRVIFK LSYIYYLNLN EDEVQYLLEK TGLSPEALKE KILHLRAELS NREEENIRME DKITSLYLNI LELKEKQQNT AKIAPILPME VDKTSHALKK KYEQRKRLLE KKKKGHFLAR TPYREVAELI GISEGNVSVT LLRLIEKIQK XXDFSDLDF
Validated NT Sequence
atcttactca cttaaaaatc caaatcactg aaatccnnnt ttttctgaat tttttcaatc aaacgaagta aagtaacgct tacgttcccc tcagaaatcc caattaattc tgcaacttct ctgtaagggg ttcttgctaa aaagtgtcct ttctttttct tttccaaaag tcttttacgc tgctcatatt tctttttcaa agcgtgagaa gttttatcca cttccatagg aagaatagga gctattttag cagtattttg ctgtttttct ttcaattcta atatatttag atataaagag gtaattttat cttccattcg aatattttct tcttcccgat tggaaagttc cgctctgaga tgaagaattt tctctttcaa tgcctcagga gataatcctg ttttttccag aagatactga acttcatctt cgtttaagtt taaataataa atataagaaa gtttaaaaat gacccgttta tcaaccccaa tttcttccag agcctgattg aatcggtcag aaaattcact ggcttcttcc tgcatttcag gacgtgtatc cggttcgtct tctatggttg cgtattcctt cccttcttta ctaatttttc ctaaattggt aaccttaagt tctcgcttgg ttctttgcca atcaatcagc atatttctaa gaacggaata gaaccaagtt ctaaaactgg attttccctt aaaacttcca aaacgggagc ctgtttttaa tctttcaaat gcatagataa aaaaatcccc agcgtcgtct tcgctcaaat gaaagatttt cattgggaaa ttgtagatat cttccgaata gatttcgaag aattgtctca gcgcatcttc ttctccatct ccacatcgag aaacgagatc taaggtttta tcttttgaca aattcatatg gtggtggtgg tggtgagcca tggta
Expected Protein Sequence
MAHHHHHHMN LSKDKTLDLV SRCGDGEEDA LRQFFEIYSE DIYNFPMKIF HLSEDDAGDF FIYAFERLKT GSRFGSFKGK SSFRTWFYSV LRNMLIDWQR TKRELKVTNL GKISKEGKEY ATIEDEPDTR PEMQEEASEF SDRFNQALEE IGVDKRVIFK LSYIYYLNLN EDEVQYLLEK TGLSPEALKE KILHLRAELS NREEENIRME DKITSLYLNI LELKEKQQNT AKIAPILPME VDKTSHALKK KYEQRKRLLE KKKKGHFLAR TPYREVAELI GISEGNVSVT LLRLIEKIQK KLDFSDLDF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt tgtcaaaaga taaaacctta gatctcgttt ctcgatgtgg agatggagaa gaagatgcgc tgagacaatt cttcgaaatc tattcggaag atatctacaa tttcccaatg aaaatctttc atttgagcga agacgacgct ggggattttt ttatctatgc atttgaaaga ttaaaaacag gctcccgttt tggaagtttt aagggaaaat ccagttttag aacttggttc tattccgttc ttagaaatat gctgattgat tggcaaagaa ccaagcgaga acttaaggtt accaatttag gaaaaattag taaagaaggg aaggaatacg caaccataga agacgaaccg gatacacgtc ctgaaatgca ggaagaagcc agtgaatttt ctgaccgatt caatcaggct ctggaagaaa ttggggttga taaacgggtc atttttaaac tttcttatat ttattattta aacttaaacg aagatgaagt tcagtatctt ctggaaaaaa caggattatc tcctgaggca ttgaaagaga aaattcttca tctcagagcg gaactttcca atcgggaaga agaaaatatt cgaatggaag ataaaattac ctctttatat ctaaatatat tagaattgaa agaaaaacag caaaatactg ctaaaatagc tcctattctt cctatggaag tggataaaac ttctcacgct ttgaaaaaga aatatgagca gcgtaaaaga cttttggaaa agaaaaagaa aggacacttt ttagcaagaa ccccttacag agaagttgca gaattaattg ggatttctga ggggaacgta agcgttactt tacttcgttt gattgaaaaa attcagaaaa agctggattt cagtgatttg gatttttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t