SSGCID: Target Page

LpinA.19661.b

Histidine kinase (EC 2.7.13.3)

CENTER ID:	LpinA.19661.b
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19661.b.B1.GE41903	full length		1	459

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19661.b.B1.PS38462	full length		1	459

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.3149
UniProt:	Q72P49

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDMLQKPKIL VVEDEIIVAV NLGQKLKKLG YELVGITSSG EEAIQKAEEN HPDLVLMDIN IEGNLDGIET AEVLRNRFHT PVIYLTAYAD ESTLDRAKKT EPLGYIVKPF ESDQLRSSIE VALYKNEIEQ RSKQTEEKLK GALDQLGAGI VTTDENGLLL FMNPAAEKLI GCKYEDYLGK PIRQILFFRN GSGNTVENLV EEVLKSGLPK DNGNLFLISK NGSSHEIQFQ SSPIFGGEEK LTGTFNILRT KEQHTNNAEK DTYLKEIHHR IKNNLTIISS IFSLRSGQTT GESNDVLRES QNRLRAVALL HEILYESKDL SLISFELYVK KLTDALFQIY QVNREKVRLE LNIQEGKVKA EIGMNLALII NELITNSLKH GLANSESGSI RVHFTKENDI LVLEVSDSGN GLPEESIRKR NPSSLGLSLV ESLAKQIGGK IDFINQEGAC VQLRFPVSA

NT Sequence

atggatatgc ttcaaaaacc caaaatattg gtggtcgaag acgagatcat cgttgccgta aacctaggac aaaaactaaa aaagctaggt tatgagcttg taggaatcac atcatctggg gaagaagcaa tccagaaagc ggaagaaaat catccggatt tagttctcat ggatataaac atagaaggaa atttggacgg aattgaaacc gccgaagtac ttcgaaatcg gtttcataca cccgttattt atttaaccgc atacgcagat gaaagcactt tagatagggc caaaaaaacg gaacctttag ggtatatcgt aaaacctttt gaatcggatc aactccgctc atccatcgaa gtcgcccttt ataaaaatga aattgaacaa agatcaaaac aaacggaaga aaaattaaaa ggtgctctcg accaacttgg agcaggaatc gtaactaccg acgaaaacgg gcttcttctt tttatgaatc cggcggctga aaaattgatc ggttgtaaat atgaagatta tttaggaaaa ccgattcgac aaattttgtt tttcagaaat gggtctggca atacggtcga aaacttagta gaagaagtac tcaaatctgg acttcccaaa gataatggaa atctattttt aatttccaaa aacggaagta gccatgaaat tcaatttcaa agttctccta tctttggagg agaagaaaaa ctaaccggaa cttttaacat tcttagaacc aaagaacaac atacaaataa cgccgaaaaa gatacttatc tcaaagaaat tcatcataga atcaaaaaca atcttacgat catctcctct atctttagtc ttagatcggg tcaaacaact ggagaatcta acgacgttct tcgagaaagt caaaatcgtc taagagcggt ggctcttctt cacgaaattc tctatgaaag taaagatctt tctttgatca gttttgaact ctacgtaaaa aaacttacag atgcactttt tcaaatctat caagtaaata gagaaaaagt tcgtctggaa ctcaatatcc aagaaggcaa ggtaaaagcc gagattggta tgaacctcgc tttgattatc aacgaactta ttacaaattc gttaaaacac ggactggcga attctgaatc tggttcgatt cgagttcatt ttacaaagga gaacgacatt ttagtccttg aagtttctga cagcggaaac ggtcttcccg aagaatcaat tcgaaaaaga aacccaagtt ctcttggact atctcttgtg gaatctcttg caaaacaaat cggcggtaaa atcgatttta tcaatcaaga aggcgcttgc gttcagcttc gttttccagt ttcagcttaa

Details for LpinA.19661.b.B1.GE41903

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	52kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMD MLQKPKILVV EDEIIVAVNL GQKLKKLGYE LVGITSSGEE AIXXAEENHP DLVLMDINIE GNLDGIETAE VLRNRFHTPV IYLTAYADES TLDRAKKTEP LGYIVKPFES DQLRSSIEVA LYKNEIEQRS KQTEEKLKGA LDQLGAGIVT TDENGLLLFM NPAAEKLIGC KYEDYXGKPI RQILFFRNGS GNTVENLVEE VLKSGLPKDN GNLFLISKNG SSHEIQFQSS PIFGGEEKLT GTFNILRTKE QHTNNAEKDT YLKEIHHRIK NNLTIISSIF SLRSGQTTGE SNDVLRESQN RLRAVALLHE ILYESKDLSL ISFELYVKKL TDALFQXYQV NREKVRLELN IQEGKVKAEI GMNLALIINE LITNSLKHGL ANSESGSIRV HFTKENDILV LEVSDSGNGL PEESIRKRNP SSLGLSLVES LAKQIGGKID FINQEGAC

Validated NT Sequence

cgcaagcgcc ttcttgattg ataaaatcga ttttaccgcc gatttgtttt gcaagagatt ccacaagaga tagtccaaga gaacttgggt ttctttttcg aattgattct tcgggaagac cgtttccgct gtcagaaact tcaaggacta aaatgtcgtt ctcctttgta aaatgaactc gaatcgaacc agattcagaa ttcgccagtc cgtgttttaa cgaatttgta ataagttcgt tgataatcaa agcgaggttc ataccaatct cggcttttac cttgccttct tggatattga gttccagacg aactttttct ctatttactt gatagntttg aaaaagtgca tctgtaagtt tttttacgta gagttcaaaa ctgatcaaag aaagatcttt actttcatag agaatttcgt gaagaagagc caccgctctt agacgatttt gactttctcg aagaacgtcg ttagattctc cagttgtttg acccgatcta agactaaaga tagaggagat gatcgtaaga ttgtttttga ttctatgatg aatttctttg agataagtat ctttttcggc gttatttgta tgttgttctt tggttctaag aatgttaaaa gttccggtta gtttttcttc tcctccaaag ataggagaac tttgaaattg aatttcatgg ctacttccgt ttttggaaat taaaaataga tttccattat ctttgggaag tccagatttg agtacttctt ctactaagtt ttcgaccgta ttgccagacc catttctgaa aaacaaaatt tgtcgaatcg gttttccnaa ataatcttca tatttacaac cgatcaattt ttcagccgcc ggattcataa aaagaagaag cccgttttcg tcggtagtta cgattcctgc tccaagttgg tcgagagcac cttttaattt ttcttccgtt tgttttgatc tttgttcaat ttcattttta taaagggcga cttcgatgga tgagcggagt tgatccgatt caaaaggttt tacgatatac cctaaaggtt ccgttttttt ggccctatct aaagtgcttt catctgcgta tgcggttaaa taaataacgg gtgtatgaaa ccgatttcga agtacttcgg cggtttcaat tccgtccaaa tttccttcta tgtttatatc catgagaact aaatccggat gattttcttc cgcttnntgg attgcttctt ccccagatga tgtgattcct acaagctcat aacctagctt ttttagtttt tgtcctaggt ttacggcaac gatgatctcg tcttcgacca ccaatatttt gggtttttga agcatatcca tatggtggtg gtggtggtga gccatggta

Expected Protein Sequence

MAHHHHHHMD MLQKPKILVV EDEIIVAVNL GQKLKKLGYE LVGITSSGEE AIQKAEENHP DLVLMDINIE GNLDGIETAE VLRNRFHTPV IYLTAYADES TLDRAKKTEP LGYIVKPFES DQLRSSIEVA LYKNEIEQRS KQTEEKLKGA LDQLGAGIVT TDENGLLLFM NPAAEKLIGC KYEDYLGKPI RQILFFRNGS GNTVENLVEE VLKSGLPKDN GNLFLISKNG SSHEIQFQSS PIFGGEEKLT GTFNILRTKE QHTNNAEKDT YLKEIHHRIK NNLTIISSIF SLRSGQTTGE SNDVLRESQN RLRAVALLHE ILYESKDLSL ISFELYVKKL TDALFQIYQV NREKVRLELN IQEGKVKAEI GMNLALIINE LITNSLKHGL ANSESGSIRV HFTKENDILV LEVSDSGNGL PEESIRKRNP SSLGLSLVES LAKQIGGKID FINQEGACVQ LRFPVSA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata tgcttcaaaa acccaaaata ttggtggtcg aagacgagat catcgttgcc gtaaacctag gacaaaaact aaaaaagcta ggttatgagc ttgtaggaat cacatcatct ggggaagaag caatccagaa agcggaagaa aatcatccgg atttagttct catggatata aacatagaag gaaatttgga cggaattgaa accgccgaag tacttcgaaa tcggtttcat acacccgtta tttatttaac cgcatacgca gatgaaagca ctttagatag ggccaaaaaa acggaacctt tagggtatat cgtaaaacct tttgaatcgg atcaactccg ctcatccatc gaagtcgccc tttataaaaa tgaaattgaa caaagatcaa aacaaacgga agaaaaatta aaaggtgctc tcgaccaact tggagcagga atcgtaacta ccgacgaaaa cgggcttctt ctttttatga atccggcggc tgaaaaattg atcggttgta aatatgaaga ttatttagga aaaccgattc gacaaatttt gtttttcaga aatgggtctg gcaatacggt cgaaaactta gtagaagaag tactcaaatc tggacttccc aaagataatg gaaatctatt tttaatttcc aaaaacggaa gtagccatga aattcaattt caaagttctc ctatctttgg aggagaagaa aaactaaccg gaacttttaa cattcttaga accaaagaac aacatacaaa taacgccgaa aaagatactt atctcaaaga aattcatcat agaatcaaaa acaatcttac gatcatctcc tctatcttta gtcttagatc gggtcaaaca actggagaat ctaacgacgt tcttcgagaa agtcaaaatc gtctaagagc ggtggctctt cttcacgaaa ttctctatga aagtaaagat ctttctttga tcagttttga actctacgta aaaaaactta cagatgcact ttttcaaatc tatcaagtaa atagagaaaa agttcgtctg gaactcaata tccaagaagg caaggtaaaa gccgagattg gtatgaacct cgctttgatt atcaacgaac ttattacaaa ttcgttaaaa cacggactgg cgaattctga atctggttcg attcgagttc attttacaaa ggagaacgac attttagtcc ttgaagtttc tgacagcgga aacggtcttc ccgaagaatc aattcgaaaa agaaacccaa gttctcttgg actatctctt gtggaatctc ttgcaaaaca aatcggcggt aaaatcgatt ttatcaatca agaaggcgct tgcgttcagc ttcgttttcc agtttcagct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for LpinA.19661.b.B1.PS38462

PURIFICATION DATe:	6/19/2018
CONCENTRATION:	33.12mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATA TGCTTCAAAA ACCCAAAATA TTGGTGGTCG AAGACGAGAT CATCGTTGCC GTAAACCTAG GACAAAAACT AAAAAAGCTA GGTTATGAGC TTGTAGGAAT CACATCATCT GGGGAAGAAG CAATCCAGAA AGCGGAAGAA AATCATCCGG ATTTAGTTCT CATGGATATA AACATAGAAG GAAATTTGGA CGGAATTGAA ACCGCCGAAG TACTTCGAAA TCGGTTTCAT ACACCCGTTA TTTATTTAAC CGCATACGCA GATGAAAGCA CTTTAGATAG GGCCAAAAAA ACGGAACCTT TAGGGTATAT CGTAAAACCT TTTGAATCGG ATCAACTCCG CTCATCCATC GAAGTCGCCC TTTATAAAAA TGAAATTGAA CAAAGATCAA AACAAACGGA AGAAAAATTA AAAGGTGCTC TCGACCAACT TGGAGCAGGA ATCGTAACTA CCGACGAAAA CGGGCTTCTT CTTTTTATGA ATCCGGCGGC TGAAAAATTG ATCGGTTGTA AATATGAAGA TTATTTAGGA AAACCGATTC GACAAATTTT GTTTTTCAGA AATGGGTCTG GCAATACGGT CGAAAACTTA GTAGAAGAAG TACTCAAATC TGGACTTCCC AAAGATAATG GAAATCTATT TTTAATTTCC AAAAACGGAA GTAGCCATGA AATTCAATTT CAAAGTTCTC CTATCTTTGG AGGAGAAGAA AAACTAACCG GAACTTTTAA CATTCTTAGA ACCAAAGAAC AACATACAAA TAACGCCGAA AAAGATACTT ATCTCAAAGA AATTCATCAT AGAATCAAAA ACAATCTTAC GATCATCTCC TCTATCTTTA GTCTTAGATC GGGTCAAACA ACTGGAGAAT CTAACGACGT TCTTCGAGAA AGTCAAAATC GTCTAAGAGC GGTGGCTCTT CTTCACGAAA TTCTCTATGA AAGTAAAGAT CTTTCTTTGA TCAGTTTTGA ACTCTACGTA AAAAAACTTA CAGATGCACT TTTTCAAATC TATCAAGTAA ATAGAGAAAA AGTTCGTCTG GAACTCAATA TCCAAGAAGG CAAGGTAAAA GCCGAGATTG GTATGAACCT CGCTTTGATT ATCAACGAAC TTATTACAAA TTCGTTAAAA CACGGACTGG CGAATTCTGA ATCTGGTTCG ATTCGAGTTC ATTTTACAAA GGAGAACGAC ATTTTAGTCC TTGAAGTTTC TGACAGCGGA AACGGTCTTC CCGAAGAATC AATTCGAAAA AGAAACCCAA GTTCTCTTGG ACTATCTCTT GTGGAATCTC TTGCAAAACA AATCGGCGGT AAAATCGATT TTATCAATCA AGAAGGCGCT TGCGTTCAGC TTCGTTTTCC AGTTTCAGCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT