LpinA.19618.a

LipL36

CENTER ID: LpinA.19618.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19618.a.B2.GE41965 mature protein 24 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.3697
UniProt: Q72MX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRNIMKIAA VAALTVALTA CKGDDDDDDV VMLALLYLAD QTSGNCVTLS KADGNGDGIP TYTATGNTKP KGACNNFYNV TAIVNNPEAV VSSVKASYQA AKDKTTAAGS NCAVVSTYLQ SAIDAATTLK VQQTLATGTC SRLGTGWNLN PLTFGGNFSV DPAYLGKTVY ACSSEKAKEE LFDSFAIDFS TVAGSVATDM ATNLAYRQKF TAVSASNFKW TADAAAKGRL INVTELTTVG KSGATVVAFG AIQSAALAAV GAGNVAGALA AASCGKDLLA KEAEDVQKIA FALYDPASGF NGAMTGGTLD GTITTAQAQS ITEVLFTSLT CQYGDFDEEA TTNKQTVGTE TNVKINGTCP ATYPKI
NT Sequence
atgagaagaa acataatgaa aattgccgct gtagcagcac ttactgttgc tctaacggca tgtaaaggtg acgatgatga tgacgatgtt gttatgttgg cacttttgta tttagcagat caaacaagcg gaaactgtgt tactcttagc aaggctgacg gtaatggaga tggaatacct acttacacag caactggtaa tacgaaacca aaaggagctt gtaacaattt ctataatgta actgccattg ttaacaatcc agaagcagtc gtaagttcag ttaaagcatc ctatcaggct gctaaggata aaactacagc agctggttca aattgcgctg tagtaagcac ttatcttcaa agtgcaattg atgctgcaac gacacttaaa gtacaacaga cattagcgac tggtacttgt tcaagattgg gaacaggttg gaatctcaat cctttaactt tcggtggtaa ctttagtgta gatcctgctt atctcggaaa aaccgtttat gcttgttctt ctgaaaaagc aaaagaggaa ttatttgact cgtttgctat cgatttctca actgttgctg gatcggttgc aacggatatg gcaacgaact tggcatatag acaaaaattc actgcggttt ctgcgtctaa ctttaaatgg actgcagatg cagctgctaa aggtcgtttg atcaatgtta ctgagcttac aactgttggt aaatccggag caactgtagt tgcatttgga gctattcaat ctgcagcttt agcagcagtt ggtgctggta acgtagcagg agctcttgca gcagcttctt gtggaaaaga tctgttagct aaagaagctg aagatgttca aaaaattgct tttgcacttt atgatccagc gtctggtttt aatggtgcga tgacaggtgg aaccttggat ggtacaatta ctactgccca agcacagtct ataacagaag ttctttttac tagccttact tgtcaatatg gtgattttga tgaagaggca acaactaata aacaaacagt tggaactgaa acaaacgtaa aaattaacgg aacttgccct gcaacttatc ctaaaatcta a
Details for LpinA.19618.a.B2.GE41965
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMD DDDDDVVMLA LLYLADQTSG NCVTLSKADG NGDGIPTYTA TGNTKPKGAC NNFYNVTAIV NNPEAVVSSV KASYQAAKDK TTAAGSNCAV VSTYLQSAID AATTLKVQQT LATGTCSRLG TGWNLNPLTF GGNFSVDPAY LGKTVYACSS EKAKEELFDS FAIDFSTVAG SVATDMATNL AYRQKFTAVS ASNFKWTADA AAKGRLINVT ELTTVGKSGA TVVAFGAIQS AALAAVGAGN VAGALAAASC GKDLLAKEAE DVQKIAFALY DPASGFNGAM TGGTLDGTIT TAQAQSITEV LFTSLTCQYG DFDEEATTNK QTVGTETNVK IN
Validated NT Sequence
tccgttaatt tttacgtttg tttcagttcc aactgtttgt ttattagttg ttgcctcttc atcaaaatca ccatattgac aagtaaggct agtaaaaaga acttctgtta tagactgtgc ttgggcagta gtaattgtac catccaaggt tccacctgtc atcgcaccat taaaaccaga cgctggatca taaagtgcaa aagcaatttt ttgaacatct tcagcttctt tagctaacag atcttttcca caagaagctg ctgcaagagc tcctgctacg ttaccagcac caactgctgc taaagctgca gattgaatag ctccaaatgc aactacagtt gctccggatt taccaacagt tgtaagctca gtaacattga tcaaacgacc tttagcagct gcatctgcag tccatttaaa gttagacgca gaaaccgcag tgaatttttg tctatatgcc aagttcgttg ccatatccgt tgcaaccgat ccagcaacag ttgagaaatc gatagcaaac gagtcaaata attcctcttt tgctttttca gaagaacaag cataaacggt ttttccgaga taagcaggat ctacactaaa gttaccaccg aaagttaaag gattgagatt ccaacctgtt cccaatcttg aacaagtacc agtcgctaat gtctgttgta ctttaagtgt cgttgcagca tcaattgcac tttgaagata agtgcttact acagcgcaat ttgaaccagc tgctgtagtt ttatccttag cagcctgata ggatgcttta actgaactta cgactgcttc tggattgtta acaatggcag ttacattata gaaattgtta caagctcctt ttggtttcgt attaccagtt gctgtgtaag taggtattcc atctccatta ccgtcagcct tgctaagagt aacacagttt ccgcttgttt gatctgctaa atacaaaagt gccaacataa caacatcgtc atcatcatcg tccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHDD DDDDVVMLAL LYLADQTSGN CVTLSKADGN GDGIPTYTAT GNTKPKGACN NFYNVTAIVN NPEAVVSSVK ASYQAAKDKT TAAGSNCAVV STYLQSAIDA ATTLKVQQTL ATGTCSRLGT GWNLNPLTFG GNFSVDPAYL GKTVYACSSE KAKEELFDSF AIDFSTVAGS VATDMATNLA YRQKFTAVSA SNFKWTADAA AKGRLINVTE LTTVGKSGAT VVAFGAIQSA ALAAVGAGNV AGALAAASCG KDLLAKEAED VQKIAFALYD PASGFNGAMT GGTLDGTITT AQAQSITEVL FTSLTCQYGD FDEEATTNKQ TVGTETNVKI NGTCPATYPK I
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgatg atgatgacga tgttgttatg ttggcacttt tgtatttagc agatcaaaca agcggaaact gtgttactct tagcaaggct gacggtaatg gagatggaat acctacttac acagcaactg gtaatacgaa accaaaagga gcttgtaaca atttctataa tgtaactgcc attgttaaca atccagaagc agtcgtaagt tcagttaaag catcctatca ggctgctaag gataaaacta cagcagctgg ttcaaattgc gctgtagtaa gcacttatct tcaaagtgca attgatgctg caacgacact taaagtacaa cagacattag cgactggtac ttgttcaaga ttgggaacag gttggaatct caatccttta actttcggtg gtaactttag tgtagatcct gcttatctcg gaaaaaccgt ttatgcttgt tcttctgaaa aagcaaaaga ggaattattt gactcgtttg ctatcgattt ctcaactgtt gctggatcgg ttgcaacgga tatggcaacg aacttggcat atagacaaaa attcactgcg gtttctgcgt ctaactttaa atggactgca gatgcagctg ctaaaggtcg tttgatcaat gttactgagc ttacaactgt tggtaaatcc ggagcaactg tagttgcatt tggagctatt caatctgcag ctttagcagc agttggtgct ggtaacgtag caggagctct tgcagcagct tcttgtggaa aagatctgtt agctaaagaa gctgaagatg ttcaaaaaat tgcttttgca ctttatgatc cagcgtctgg ttttaatggt gcgatgacag gtggaacctt ggatggtaca attactactg cccaagcaca gtctataaca gaagttcttt ttactagcct tacttgtcaa tatggtgatt ttgatgaaga ggcaacaact aataaacaaa cagttggaac tgaaacaaac gtaaaaatta acggaacttg ccctgcaact tatcctaaaa tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat