LpinA.19611.a

2-dehydropantoate 2-reductase (EC 1.1.1.169) (Ketopantoate reductase)

CENTER ID: LpinA.19611.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19611.a.B2.GE41963 mature protein 24 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.2088
UniProt: Q72RL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFRILVLGSG AIAGLYAGKL VQAGCKVDFW VRKNSYELKR NGFQIESVPW GNFHYKVEKI FESIPKNLKE YDLILNCLKC LPDINLKKIL GEKIPPNLPI LLLQNGIGIE EPVSILYPEN EILSGLAFVC ANRLDAGKIL HLDYGELTIG SWNRNPSFIC DQLVNLFNSV GVPTQNTNTI RQACWKKLMW NAPFNPISVL CGGKNTLEIL ENPHSCKLVI EIMKEVQTLS KLDGAEVPST QIDIFLQMTK TMKPYKTSML LDFEA
NT Sequence
ttgtttcgaa ttttagtttt aggttccggt gctatcgccg gtctatacgc cggtaaactt gtacaagcgg gttgtaaggt tgatttttgg gttcgaaaaa attcttacga attaaaaaga aatggctttc aaattgaaag cgttccttgg ggaaattttc attataaggt agaaaagatt tttgaaagta ttcccaaaaa cttaaaagaa tacgatctga ttttaaattg tttaaaatgt cttcctgata ttaacctgaa aaaaattcta ggggaaaaaa tccctccgaa tctaccaatt ttacttttac aaaatggaat tggtatcgaa gaaccggttt ctattcttta tcccgaaaat gaaattttaa gcggacttgc ttttgtatgt gccaatcggc ttgatgctgg aaaaattctt catttagatt atggagaatt gacgattgga tcttggaaca gaaatccttc ttttatctgt gatcaattag taaatctttt taatagcgtg ggagttccca cacaaaatac aaatacaatc cgtcaagctt gttggaaaaa actgatgtgg aacgcgccct tcaatcctat cagtgttctt tgtggcggta aaaatacttt agaaatttta gaaaacccac atagttgtaa actcgtaatt gaaattatga aagaggtaca gaccctttct aaattagacg gagcggaagt tccttcaact cagatagata tttttttaca gatgacaaaa acgatgaaac cttataaaac tagtatgctt ttagattttg aagcgtag
Details for LpinA.19611.a.B2.GE41963
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG CKVDFWVRKN SYELKRNGFQ IESVPWGNFH YKVEKIFESI PKNLKEYDLI LNCLKCLPDI NLKKILGEKI PPNLPILLLQ NGIGIEEPVS ILYPENEILS GLAFVCANRL DAGKILHLDY GELTIGSWNR NPSFICDQLV NLFNSVGVPT QNTNTIRQAC WKKLMWNAPF NPISVLCGGK NTLEILENPH SCKLVIEIMK EVQTLSKLDG AEVPSTQIDI FLQMTKTMKP YXXXXF
Validated NT Sequence
tcacttacgc ttcaaaatct aaaagcnnnn nnnnttataa ggtttcatcg tttttgtcat ctgtaaaaaa atatctatct gagttgaagg aacttccgct ccgtctaatt tagaaagggt ctgtacctct ttcataattt caattacgag tttacaacta tgtgggtttt ctaaaatttc taaagtattt ttaccgccac aaagaacact gataggattg aagggcgcgt tccacatcag ttttttccaa caagcttgac ggattgtatt tgtattttgt gtgggaactc ccacgctatt aaaaagattt actaattgat cacagataaa agaaggattt ctgttccaag atccaatcgt caattctcca taatctaaat gaagaatttt tccagcatca agccgattgg cacatacaaa agcaagtccg cttaaaattt cattttcggg ataaagaata gaaaccggtt cttcgatacc aattccattt tgtaaaagta aaattggtag attcggaggg attttttccc ctagaatttt tttcaggtta atatcaggaa gacattttaa acaatttaaa atcagatcgt attcttttaa gtttttggga atactttcaa aaatcttttc taccttataa tgaaaatttc cccaaggaac gctttcaatt tgaaagccat ttctttttaa ttcgtaagaa ttttttcgaa cccaaaaatc aaccttacaa cccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHGC KVDFWVRKNS YELKRNGFQI ESVPWGNFHY KVEKIFESIP KNLKEYDLIL NCLKCLPDIN LKKILGEKIP PNLPILLLQN GIGIEEPVSI LYPENEILSG LAFVCANRLD AGKILHLDYG ELTIGSWNRN PSFICDQLVN LFNSVGVPTQ NTNTIRQACW KKLMWNAPFN PISVLCGGKN TLEILENPHS CKLVIEIMKE VQTLSKLDGA EVPSTQIDIF LQMTKTMKPY KTSMLLDFEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggttgta aggttgattt ttgggttcga aaaaattctt acgaattaaa aagaaatggc tttcaaattg aaagcgttcc ttggggaaat tttcattata aggtagaaaa gatttttgaa agtattccca aaaacttaaa agaatacgat ctgattttaa attgtttaaa atgtcttcct gatattaacc tgaaaaaaat tctaggggaa aaaatccctc cgaatctacc aattttactt ttacaaaatg gaattggtat cgaagaaccg gtttctattc tttatcccga aaatgaaatt ttaagcggac ttgcttttgt atgtgccaat cggcttgatg ctggaaaaat tcttcattta gattatggag aattgacgat tggatcttgg aacagaaatc cttcttttat ctgtgatcaa ttagtaaatc tttttaatag cgtgggagtt cccacacaaa atacaaatac aatccgtcaa gcttgttgga aaaaactgat gtggaacgcg cccttcaatc ctatcagtgt tctttgtggc ggtaaaaata ctttagaaat tttagaaaac ccacatagtt gtaaactcgt aattgaaatt atgaaagagg tacagaccct ttctaaatta gacggagcgg aagttccttc aactcagata gatatttttt tacagatgac aaaaacgatg aaaccttata aaactagtat gcttttagat tttgaagcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat