LpinA.19588.a

Uncharacterized protein

CENTER ID: LpinA.19588.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19588.a.B2.GE42924 mature protein 31 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.1349
UniProt: Q72TD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYTKNRKRG LRSGLILSIL IFLSVNVCTS VQNGTEGIEN RFVYVENVQG KNEEDFELNA KRKILEKGLG ELIESGSQTI DGQIKETVTN SSIEGYVTEF SRMGSFRKIG NLLEADAKGK VSRKLIEDSL KERYKELGKP KFLMIIDETI LGKSNNSISE NAIVKKFIEF DFLEKSQLAR VLTKQSGKSV TIYGNQTLET EVLSTASEMG AQILLVGQTQ VTNAGEIENS ELKSYQAVIR FKIYDVNTKR IIAADNTSGA VLHVNPDTGI QEAIQKAVEK IYPKIREQIS IKWKPGNLIL LKIEGISYDD YVDKDIKGLI RGIKGVNRVS EVNVNQPIVL EIEALYNGNN LYQKMRERKT DFGFDFSQKE IKSSLIHIIK K
NT Sequence
atgaaatata caaaaaatag gaagagggga ctcagatcgg gtctgatact tagcattttg atttttttat cagttaatgt ttgtacttcc gtacaaaatg gaactgaagg tattgagaat agatttgtat atgtagaaaa tgttcaaggt aaaaacgaag aagacttcga gctcaacgca aaaagaaaaa tattggaaaa aggattgggg gaactgatcg agagtggatc acagacaatc gatggacaaa tcaaagagac ggtaacaaat tcttctatcg aaggatatgt aactgaattt tctagaatgg gatcttttcg caaaatagga aacttattgg aagcagacgc aaagggaaaa gtaagtcgta aattgatcga agactcatta aaagaaagat ataaagaatt aggaaaacct aaatttttga tgattataga cgaaacgatc ttaggaaaat caaataattc tatatcggaa aatgcgatcg taaagaaatt catagaattt gattttctgg aaaaaagtca attggctcga gttttaacaa aacaaagcgg aaaatcggtc acaatttacg gaaatcaaac actcgaaaca gaggtattat ccactgcatc agaaatgggg gctcagatat tattggtcgg acaaacacaa gtgacaaatg ccggagagat tgaaaatagc gaattaaaat catatcaagc agtaattcgg tttaaaattt atgatgttaa tacgaaaaga atcatagcag ctgataatac aagcggggcc gttttgcacg taaacccaga tacaggaatt caagaagcaa ttcaaaaagc cgttgaaaaa atatatccta aaattcgaga acaaatatca ataaaatgga aaccggggaa tttgatattg cttaaaatag aaggaatctc ttatgacgac tatgtagata aagatataaa agggctgatc cgagggataa aaggtgtcaa tagagtttcc gaagttaatg tcaatcagcc tattgtttta gaaatagaag cgttgtataa tggaaataat ctatatcaga aaatgagaga gagaaaaaca gactttggtt ttgacttttc gcaaaaagaa atcaaatcga gtctgattca cataattaaa aaataa
Details for LpinA.19588.a.B2.GE42924
HARVESTED ON: 2/7/2019
SEQUENCED ON: 2/8/2019
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHVQ NGTEGIENRF VYVENVQGKN EEDFELNAKR KILEKGLGEL IESGSQTIDG QIKETVTNSS IEGYVTEFSR MGSFRKIGNL LEADAKGKVS RKLIEDSLKE RYKELGKPKF LMIIDETILG KSNNSISENA IVKKFIEFDF LEKSQLARVL TKQSGKSVTI YGNQTLETEV LSTASEMGAQ ILLVGQTQVT NAGEIENSEL KSYQAVIRFK IYDVNTKRII AADNTSGAVL HVNPDTGIQE AIQKAVEKIY PKIREQISIK WKPGNLILLK IEGISYDDYV DKDIKGLIRG IKGVNRVSEV NVNQPIVLEI EALYNGNNLY QKMRERKTDF GFDFSQKEIK SSLIHIIKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtacaaa atggaactga aggtattgag aatagatttg tatatgtaga aaatgttcaa ggtaaaaacg aagaagactt cgagctcaac gcaaaaagaa aaatattgga aaaaggattg ggggaactga tcgagagtgg atcacagaca atcgatggac aaatcaaaga gacggtaaca aattcttcta tcgaaggata tgtaactgaa ttttctagaa tgggatcttt tcgcaaaata ggaaacttat tggaagcaga cgcaaaggga aaagtaagtc gtaaattgat cgaagactca ttaaaagaaa gatataaaga attaggaaaa cctaaatttt tgatgattat agacgaaacg atcttaggaa aatcaaataa ttctatatcg gaaaatgcga tcgtaaagaa attcatagaa tttgattttc tggaaaaaag tcaattggct cgagttttaa caaaacaaag cggaaaatcg gtcacaattt acggaaatca aacactcgaa acagaggtat tatccactgc atcagaaatg ggggctcaga tattattggt cggacaaaca caagtgacaa atgccggaga gattgaaaat agcgaattaa aatcatatca agcagtaatt cggtttaaaa tttatgatgt taatacgaaa agaatcatag cagctgataa tacaagcggg gccgttttgc acgtaaaccc agatacagga attcaagaag caattcaaaa agccgttgaa aaaatatatc ctaaaattcg agaacaaata tcaataaaat ggaaaccggg gaatttgata ttgcttaaaa tagaaggaat ctcttatgac gactatgtag ataaagatat aaaagggctg atccgaggga taaaaggtgt caatagagtt tccgaagtta atgtcaatca gcctattgtt ttagaaatag aagcgttgta taatggaaat aatctatatc agaaaatgag agagagaaaa acagactttg gttttgactt ttcgcaaaaa gaaatcaaat cgagtctgat tcacataatt aaaaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t