LpinA.19575.a

Sphingomyelinase C

CENTER ID: LpinA.19575.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19575.a.B2.GE42914 mature protein 38 554
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.795
UniProt: Q72UK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRNIFRKKNN EIRNTYKKSK SLIGFYCLFI FFLNCLPDKQ KVHADLLMSL LLFSKDQNIQ FANISAERIG IDAQVSSEVD IKILSYNLFM YHKGLFNSGN WGQEKRARLL VDSKYVKDQD VLVLEGVFDT KSKNILMNGL RSQYPNQIDV IGRATYGWNQ TLGDFRAFPL DNDYNGGVIV LSKWPIEEKI QYIFENHGCG NDYYYNKGFV YVKINKHGRK YHIIGTDVQN TSCSDLGQNA RKHQFVEIKN FINLKQVPKN ETIFLAGSFN VNKGSAEYQD ILMILGVDEP HYAGISFTWD PKKNAIASYT SGTNQVSSYL EYIFVVGTHF KPQVWQNLAY DPITPTTWNV VGYTGYEFSD RYPVYGFTYA DSNTPTQSAH RRKYDQVSFE SMSTGKKIQA DPKQANGWLQ VNASDETEFT KFNLLQEADP KSDTNCLKSG YTRIEPSYYL NYYWNWWLGG GKGNYAYYPK FNDASNNLQI HVLDEEECIK DGSRIVFKDY DGATLDYYYL TIWDGGNWDG YLYLWNKTYN LRETFKVRLN TKPERDWKDD LIYR
NT Sequence
atgcgaaaca ttttccgaaa aaaaaataat gaaatacgaa atacatacaa gaaatcgaaa tctttaatag gtttttattg tctctttata ttctttttga attgtctacc ggataaacaa aaagtacatg cagatttact aatgtctctt ttactttttt caaaagatca aaatattcaa tttgcaaata taagtgcaga aagaatagga attgatgctc aagtatcttc ggaagtggat ataaaaattc taagttacaa tctttttatg tatcataaag gactttttaa ttctggaaat tggggtcaag aaaaaagagc acggttgctt gtagattcaa aatatgttaa agatcaagat gtacttgtac tggaaggagt ttttgataca aaatcgaaaa atatacttat gaatggactc cgctctcaat acccaaatca aatcgatgtt attggaagag ctacatatgg ttggaatcaa actctaggtg attttagagc ttttccgctg gataacgatt ataatggagg agtgattgtc ttaagcaaat ggcctattga agaaaaaata caatatatct ttgaaaatca cggatgtgga aacgactact attataacaa aggatttgtt tatgtaaaaa ttaataaaca tggtcgcaaa tatcatatca ttggaactga cgttcaaaat acttcttgtt cggatttggg acaaaatgca agaaaacatc agtttgtaga gattaaaaat tttatcaatt taaaacaagt gccaaaaaac gaaacaatat ttctggctgg aagttttaac gtaaataaag gtagtgcaga atatcaagat attctaatga ttttaggtgt agatgaacct cattacgcag gaatttcttt tacttgggat cctaaaaaaa atgcaattgc ttcttacacc agtggtacaa atcaagtatc cagttatttg gagtatatat ttgttgtagg aactcatttt aaacctcaag tttggcaaaa tttggcatat gatcccataa ccccgactac ctggaatgtt gtcggataca caggttatga gttttccgat cgatatcctg tttacggttt tacttatgct gattctaata ctccaactca atccgcacac agaagaaaat acgaccaagt atcttttgaa tcaatgtcaa ctggaaaaaa aattcaagct gatcctaaac aagcaaatgg ttggttacaa gtaaacgcat cagatgaaac tgaatttaca aagttcaatc tgctgcagga agccgatcct aaatccgata ccaattgtct caaaagtggg tatactcgga ttgaaccttc ttattatcta aattattatt ggaattggtg gctcggagga ggtaagggca attatgctta ctatcctaaa tttaacgatg cttctaataa tttacaaatt catgttcttg acgaagaaga atgtataaaa gacggaagta gaattgtatt caaagattat gatggagcta ctctggatta ttattatctt acgatttggg atggtggtaa ttgggatgga tatttatatc tttggaataa aacttacaat ttaagggaaa ctttcaaagt tagattgaac acaaaacctg aaagagattg gaaagacgat ttaatttatc gttag
Details for LpinA.19575.a.B2.GE42914
HARVESTED ON: 2/7/2019
SEQUENCED ON: 2/8/2019
EXPECTED MW: 61kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDK QKVHADLLMS LLLFSKDQNI QFANISAERI GIDAQVSSEV DIKILSYNLF MYHKGLFNSG NWGQEKRARL LVDSKYVKDQ DVLVLEGVFD TKSKNILMNG LRSQYPNQID VIGRATYGWN QTLGDFRAFP LDNDYNGGVI VLSKWPIEEK IQYIFENHGC GNDYYYNKGF VYVKINKHGR KYHIIGTDVQ NTSCSDLGQN ARKHQFVEIK NFINLKQVPK NETIFLAGSF NVNKGSAEYQ DILMILGVDE PHYAGISFTW DPKKNAIASY TSGTNQVSSY LEYIFVVGTH FKPQVWQNLA YDPITPTTWN VVGYTGYEFS DRYPVYGFTY ADSNTPTQSA HRRKYDQVSF ESMSTGKKIQ ADPKQANGWL QVNASDETEF TKFNLLQEAD PKSDTNCLKS GYTRIEPSYY LNYYWNWWLG GGKGNYAYYP KFNDASNNLQ IHVLDEEECI KDGSRIVFKD YDGATLDYYY LTIWDGGNWD GYLYLWNKTY NLRETFKVRL NTKPERDWKD DLIYR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgataaac aaaaagtaca tgcagattta ctaatgtctc ttttactttt ttcaaaagat caaaatattc aatttgcaaa tataagtgca gaaagaatag gaattgatgc tcaagtatct tcggaagtgg atataaaaat tctaagttac aatcttttta tgtatcataa aggacttttt aattctggaa attggggtca agaaaaaaga gcacggttgc ttgtagattc aaaatatgtt aaagatcaag atgtacttgt actggaagga gtttttgata caaaatcgaa aaatatactt atgaatggac tccgctctca atacccaaat caaatcgatg ttattggaag agctacatat ggttggaatc aaactctagg tgattttaga gcttttccgc tggataacga ttataatgga ggagtgattg tcttaagcaa atggcctatt gaagaaaaaa tacaatatat ctttgaaaat cacggatgtg gaaacgacta ctattataac aaaggatttg tttatgtaaa aattaataaa catggtcgca aatatcatat cattggaact gacgttcaaa atacttcttg ttcggatttg ggacaaaatg caagaaaaca tcagtttgta gagattaaaa attttatcaa tttaaaacaa gtgccaaaaa acgaaacaat atttctggct ggaagtttta acgtaaataa aggtagtgca gaatatcaag atattctaat gattttaggt gtagatgaac ctcattacgc aggaatttct tttacttggg atcctaaaaa aaatgcaatt gcttcttaca ccagtggtac aaatcaagta tccagttatt tggagtatat atttgttgta ggaactcatt ttaaacctca agtttggcaa aatttggcat atgatcccat aaccccgact acctggaatg ttgtcggata cacaggttat gagttttccg atcgatatcc tgtttacggt tttacttatg ctgattctaa tactccaact caatccgcac acagaagaaa atacgaccaa gtatcttttg aatcaatgtc aactggaaaa aaaattcaag ctgatcctaa acaagcaaat ggttggttac aagtaaacgc atcagatgaa actgaattta caaagttcaa tctgctgcag gaagccgatc ctaaatccga taccaattgt ctcaaaagtg ggtatactcg gattgaacct tcttattatc taaattatta ttggaattgg tggctcggag gaggtaaggg caattatgct tactatccta aatttaacga tgcttctaat aatttacaaa ttcatgttct tgacgaagaa gaatgtataa aagacggaag tagaattgta ttcaaagatt atgatggagc tactctggat tattattatc ttacgatttg ggatggtggt aattgggatg gatatttata tctttggaat aaaacttaca atttaaggga aactttcaaa gttagattga acacaaaacc tgaaagagat tggaaagacg atttaattta tcgttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat