LpinA.19564.b

Uncharacterized protein

CENTER ID: LpinA.19564.b
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19564.b.B1.GE41902 full length 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.1903
UniProt: Q72S24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKFAVSELK PGMRFSKPVY LDKENLFITS NTPVTDSDLD RLNRFGIQEV MTAGELLQLE SQYDPNVLET SLDDMIVNTV VDDELQPLKA VYDNLNRIKI TFGNLFREST QIIQDVFKKT MDEKPLEVTP VREIAERLTD FVRSNQNISY LILANNPPGY YLYNQIANST FYSLILGKLL EYSRPKMIDL GISCLLADIG MSKVPAVISE KNEQLTDEEF KTIMKHTILG YQILSQKMKL KNNLAIVALQ HHERYDGNGY PQKLAGNAIE EQARIYSISD NFSALVTNRP HRKKILPHEA IKSMISMDVG KFDLKLVRTL LNHLSLYPVG SCIELSDKRI GVVLGPNLDK PIRPFIRIIK DEYGKMVRNL ILVDLLKETN LFISRPVDLQ EISA
NT Sequence
atgaaaaaat ttgccgtatc cgaactaaag ccgggaatga gattttccaa acccgtttat ttagataagg aaaatttatt tattacttct aatactccgg tgactgacag cgatttagat cgattgaatc ggtttggaat tcaagaagtg atgactgctg gagaactact gcaactagaa tcccaatatg atccgaacgt tttagaaacg agtttagacg acatgatcgt aaatactgtc gtagacgatg aattacaacc tctcaaagcg gtttacgata atctaaatcg aattaagatt acttttggaa atttatttag agaatctact cagatcatcc aagacgtgtt taaaaaaaca atggatgaaa aacctttaga agttactcca gttcgagaaa ttgcagaaag actaaccgat tttgtacgtt ctaatcaaaa tatatcttat ttaattttag cgaacaatcc gcccggatat tatttataca atcagattgc aaattcgact ttctattctt taattttagg aaaacttcta gaatattcta gaccaaagat gatcgaccta ggaatttcct gtttacttgc agatattgga atgtcaaaag tgccagcggt gatttcggaa aaaaacgaac aactcaccga tgaggaattt aagacgatca tgaaacatac gattttagga tatcaaattc tttctcaaaa aatgaaactg aaaaataatc ttgcgattgt tgcccttcag catcatgagc gttatgatgg aaacggttat cctcaaaaat tagctggtaa tgctattgaa gaacaagcca gaatttattc tatttcagac aacttttctg cgcttgttac taatcgtcct cataggaaga agattctacc tcacgaagca attaaatcca tgattagtat ggatgtagga aagttcgatt tgaaattagt tcgaactctt cttaatcatc tttccttgta tcccgtaggt tcctgtatag agttatctga taaaaggata ggtgtcgttt tagggccgaa tttagataaa ccaattcgtc cttttattcg tattataaaa gatgaatatg gtaaaatggt tcgaaatttg atactagtgg atcttttaaa agaaacaaat ctttttattt ctcgtcccgt agatttacaa gaaatttccg cttaa
Details for LpinA.19564.b.B1.GE41902
HARVESTED ON: data unavailable
SEQUENCED ON: 9/29/2017
EXPECTED MW: 46kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK KFAVSELKPG MRFSKPVYLD KENLFITSNT PVTDSDLDRL NRFGIQEVMT AGELLQLESQ YDPNVLETSL DDMIVNTVVX DELQPLKAVY DNLNRIKITF GNLFRESTQI IQDVFKKTMD EKPLEVTPVR EIAERLTDFV RSNQNISYLI LANNPPGYYL YNQIANSTFY SLILGKLLEY SRPKMIDLGI SCLLADIGMS KVPAVISEKN EQLTDEEFKT IMKHTILGYQ ILSQKMKLKN NLAIVALQHH ERYDGNGYPQ KLAGNAIEEQ ARIYSISDNF SALVTNRPHR KKILPHEAIK SMISMDVGKF DLKLVRTLLN HLSLYPVGSC IELSDKRIGV VLGPNLDKPI RPFIRIIKDE YGKMVRNLIX VDLLKETNLF ISR
Validated NT Sequence
gacgagaaat aaaaagattt gtttctttta aaagatccac tngtatcaaa tttcgaacca ttttaccata ttcatctttt ataatacgaa taaaaggacg aattggttta tctaaattcg gccctaaaac gacacctatc cttttatcag ataactctat acaggaacct acgggataca aggaaagatg attaagaaga gttcgaacta atttcaaatc gaactttcct acatccatac taatcatgga tttaattgct tcgtgaggta gaatcttctt cctatgagga cgattagtaa caagcgcaga aaagttgtct gaaatagaat aaattctggc ttgttcttca atagcattac cagctaattt ttgaggataa ccgtttccat cataacgctc atgatgctga agggcaacaa tcgcaagatt atttttcagt ttcatttttt gagaaagaat ttgatatcct aaaatcgtat gtttcatgat cgtcttaaat tcctcatcgg tgagttgttc gtttttttcc gaaatcaccg ctggcacttt tgacattcca atatctgcaa gtaaacagga aattcctagg tcgatcatct ttggtctaga atattctaga agttttccta aaattaaaga atagaaagtc gaatttgcaa tctgattgta taaataatat ccgggcggat tgttcgctaa aattaaataa gatatatttt gattagaacg tacaaaatcg gttagtcttt ctgcaatttc tcgaactgga gtaacttcta aaggtttttc atccattgtt tttttaaaca cgtcttggat gatctgagta gattctctaa ataaatttcc aaaagtaatc ttaattcgat ttagattatc gtaaaccgct ttgagaggtt gtaattcatc gncnacgaca gtatttacga tcatgtcgtc taaactcgtt tctaaaacgt tcggatcata ttgggattct agttgcagta gttctccagc agtcatcact tcttgaattc caaaccgatt caatcgatct aaatcgctgt cagtcaccgg agtattagaa gtaataaata aattttcctt atctaaataa acgggtttgg aaaatctcat tcccggcttt agttcggata cggcaaattt tttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMK KFAVSELKPG MRFSKPVYLD KENLFITSNT PVTDSDLDRL NRFGIQEVMT AGELLQLESQ YDPNVLETSL DDMIVNTVVD DELQPLKAVY DNLNRIKITF GNLFRESTQI IQDVFKKTMD EKPLEVTPVR EIAERLTDFV RSNQNISYLI LANNPPGYYL YNQIANSTFY SLILGKLLEY SRPKMIDLGI SCLLADIGMS KVPAVISEKN EQLTDEEFKT IMKHTILGYQ ILSQKMKLKN NLAIVALQHH ERYDGNGYPQ KLAGNAIEEQ ARIYSISDNF SALVTNRPHR KKILPHEAIK SMISMDVGKF DLKLVRTLLN HLSLYPVGSC IELSDKRIGV VLGPNLDKPI RPFIRIIKDE YGKMVRNLIL VDLLKETNLF ISRPVDLQEI SA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aatttgccgt atccgaacta aagccgggaa tgagattttc caaacccgtt tatttagata aggaaaattt atttattact tctaatactc cggtgactga cagcgattta gatcgattga atcggtttgg aattcaagaa gtgatgactg ctggagaact actgcaacta gaatcccaat atgatccgaa cgttttagaa acgagtttag acgacatgat cgtaaatact gtcgtagacg atgaattaca acctctcaaa gcggtttacg ataatctaaa tcgaattaag attacttttg gaaatttatt tagagaatct actcagatca tccaagacgt gtttaaaaaa acaatggatg aaaaaccttt agaagttact ccagttcgag aaattgcaga aagactaacc gattttgtac gttctaatca aaatatatct tatttaattt tagcgaacaa tccgcccgga tattatttat acaatcagat tgcaaattcg actttctatt ctttaatttt aggaaaactt ctagaatatt ctagaccaaa gatgatcgac ctaggaattt cctgtttact tgcagatatt ggaatgtcaa aagtgccagc ggtgatttcg gaaaaaaacg aacaactcac cgatgaggaa tttaagacga tcatgaaaca tacgatttta ggatatcaaa ttctttctca aaaaatgaaa ctgaaaaata atcttgcgat tgttgccctt cagcatcatg agcgttatga tggaaacggt tatcctcaaa aattagctgg taatgctatt gaagaacaag ccagaattta ttctatttca gacaactttt ctgcgcttgt tactaatcgt cctcatagga agaagattct acctcacgaa gcaattaaat ccatgattag tatggatgta ggaaagttcg atttgaaatt agttcgaact cttcttaatc atctttcctt gtatcccgta ggttcctgta tagagttatc tgataaaagg ataggtgtcg ttttagggcc gaatttagat aaaccaattc gtccttttat tcgtattata aaagatgaat atggtaaaat ggttcgaaat ttgatactag tggatctttt aaaagaaaca aatcttttta tttctcgtcc cgtagattta caagaaattt ccgcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat