LpinA.19564.a

HD-GYP hydrolase domain protein

CENTER ID: LpinA.19564.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19564.a.B1.GE41901 full length 1 489
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19564.a.B1.PS38499 full length 1 489

Molecular models

METHOD RESULTS
comparative modelling Robetta_2676

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.151
UniProt: Q72W03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDAQKDLQKF DFTEEIIQHF KINSVIPVDF YNRNGQILIH KKENADGDDI TKLLRFESQG IYFLKSEFEK ISGGKQGDGP NNVNGRDVSF AKLVNAELTV DLAKNASNFL SELKKFPLHG NQLRHLNKSI DGILEDFKST PDMETGLVNI IEVMSSAGVP MDSEILTKRT VISMAMKVRA GKAFTKVDME QKKLDQMNLM MSSYLADVGY TQMKIPMERD LKAEEFEYIK NHPIISYLMI ANLPDLDDNI KTLVLNHHRP HKGEGMNNNY PQPKVLIHKL NVYKEKYKDD PKKTVLVADI QKQIRNILTN NLPMEDIGVI SIAGEFASLT TRQAWREAFD PLVAMKLILN NSFFAYNEKT LRDFYDHIGL SLCNNQPFIR EGDFVIVVTQ DSNQKVFFEV CIIREMYKTQ IRPMLERIGT IKPNFSNMGK LRISGFDIAS LKLDRRKAVY NLEKNQDPRR IVYVLDSNMD ARLYEELTKQ TGEIPKESA
NT Sequence
atggacgcgc agaaagactt acaaaaattc gattttacag aagaaatcat ccagcatttt aaaattaaca gcgtaattcc cgttgatttt tataatagaa acggacagat tttaattcat aaaaaggaaa atgcagacgg agatgacatt acaaaattat taagattcga gagccaggga atttactttt taaaatccga attcgaaaag atttccggcg gaaaacaagg tgatgggcca aataatgtaa atggtcgaga cgttagtttt gccaaactag tcaatgccga gttaaccgta gatcttgcta agaacgcttc caatttttta tccgaattaa aaaaatttcc ccttcatgga aatcagttaa gacatctaaa taagtccata gacggaatat tagaagattt taaatcaact ccagacatgg aaaccggatt ggttaacatc atagaagtta tgagtagcgc aggggttcct atggattccg aaatacttac aaaacgcacc gtgatttcaa tggctatgaa agtgagagcc ggaaaagctt ttactaaggt ggacatggaa cagaaaaaat tagatcaaat gaatctaatg atgtcttctt atcttgcgga tgtgggctac actcagatga aaattccgat ggagagagat ctgaaagcgg aagaatttga atatattaaa aaccatccaa ttattagtta tcttatgata gcaaatcttc cggacttgga cgataatatt aaaactttgg ttctcaatca tcaccgtcct cataaagggg aaggaatgaa taataactat cctcaaccaa aagttcttat tcataagctc aacgtttata aggaaaagta taaggacgat cctaaaaaaa ccgttttggt tgcagatatt caaaaacaaa ttcggaatat tcttaccaac aatcttccta tggaagatat tggggttatt tccattgcgg gagaatttgc atccttgacg acaagacaag cgtggagaga agcttttgat cctttggttg caatgaaatt aattttgaat aacagttttt ttgcatataa tgaaaagacc ttgagagact tttacgatca tattggtctt tcattgtgta ataatcagcc ttttattcgg gaaggtgact ttgtgattgt ggtcacacaa gattctaatc aaaaagtttt ttttgaagtt tgtattattc gagaaatgta taaaactcaa attcgtccta tgttagaaag aataggaacc attaaaccta actttagtaa tatgggtaaa cttagaattt caggatttga tattgcttct ttaaagttgg atcgtagaaa agcggtttac aatctcgaaa aaaaccaaga cccgagaagg atcgtttacg ttttggattc taatatggac gctcgtcttt atgaggaatt aactaaacaa accggcgaaa ttcctaaaga aagtgcttaa
Details for LpinA.19564.a.B1.GE41901
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 57kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD AQKDLQKFDF TEEIIQHFKI NSVIPVDFYN RNGQILIHKK ENADGDDITK LLRFESQGIY FLKSEFEKIS GGKQGDGPNN VNGRDVSFAK LVNAELTVDL AKNASNFLSE LKKFPLHGNQ LRHLNKSIDG ILEDFKSTPD METGLVNIIE VMSSAGVPMD SEILTKRTVI SMAMKVRAGK AFTKVDMEQK KLDQMNLMMS SYLADVGYTQ MKIPMERDLK AEEFEYIKNH PIISYLMIAN LPDLDDNIKT LVLNHHRPHK GEGMNNNYPQ PKVLIHKLNV YKEKYKDDPK KTVLVADIQK QIRNILTNNL PMEDIGVISI AGEFASLTTR QAWREAFDPL VAMKLILNNS FFAYNEKTLR DFYDHIGLSL CNNQPFIREG DFVIVVTQDS NQKVFFEVCI IREMYKTQIR PMLERIGTIK PNFSNMGKLR ISGFDIASLK LDRRKAVYNL EKNQDPRRIV YVLDSNMDAR LYEELTKQTN EIPKESA
Validated NT Sequence
tgtttagtta attcctcata aagacgagcg tccatattag aatccaaaac gtaaacgatc cttctcgggt cttggttttt ttcgagattg taaaccgctt ttctacgatc caactttaaa gaagcaatat caaatcctga aattctaagt ttacccatat tactaaagtt aggtttaatg gttcctattc tttctaacat aggacgaatt tgagttttat acatttctcg aataatacaa acttcaaaaa aaactttttg attagaatct tgtgtgacca caatcacaaa gtcaccttcc cgaataaaag gctgattatt acacaatgaa agaccaatat gatcgtaaaa gtctctcaag gtcttttcat tatatgcaaa aaaactgtta ttcaaaatta atttcattgc aaccaaagga tcaaaagctt ctctccacgc ttgtcttgtc gtcaaggatg caaattctcc cgcaatggaa ataaccccaa tatcttccat aggaagattg ttggtaagaa tattccgaat ttgtttttga atatctgcaa ccaaaacggt ttttttagga tcgtccttat acttttcctt ataaacgttg agcttatgaa taagaacttt tggttgagga tagttattat tcattccttc ccctttatga ggacggtgat gattgagaac caaagtttta atattatcgt ccaagtccgg aagatttgct atcataagat aactaataat tggatggttt ttaatatatt caaattcttc cgctttcaga tctctctcca tcggaatttt catctgagtg tagcccacat ccgcaagata agaagacatc attagattca tttgatctaa ttttttctgt tccatgtcca ccttagtaaa agcttttccg gctctcactt tcatagccat tgaaatcacg gtgcgttttg taagtatttc ggaatccata ggaacccctg cgctactcat aacttctatg atgttaacca atccggtttc catgtctgga gttgatttaa aatcttctaa tattccgtct atggacttat ttagatgtct taactgattt ccatgaaggg gaaatttttt taattcggat aaaaaattgg aagcgttctt agcaagatct acggttaact cggcattgac tagtttggca aaactaacgt ctcgaccatt tacattattt ggcccatcac cttgttttcc gccggaaatc ttttcgaatt cggattttaa aaagtaaatt ccctggctct cgaatcttaa taattttgta atgtcatctc cgtctgcatt ttccttttta tgaattaaaa tctgtccgtt tctattataa aaatcaacgg gaattacgct gttaatttta aaatgctgga tgatttcttc tgtaaaatcg aatttttgta agtctttctg cgcgtccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMD AQKDLQKFDF TEEIIQHFKI NSVIPVDFYN RNGQILIHKK ENADGDDITK LLRFESQGIY FLKSEFEKIS GGKQGDGPNN VNGRDVSFAK LVNAELTVDL AKNASNFLSE LKKFPLHGNQ LRHLNKSIDG ILEDFKSTPD METGLVNIIE VMSSAGVPMD SEILTKRTVI SMAMKVRAGK AFTKVDMEQK KLDQMNLMMS SYLADVGYTQ MKIPMERDLK AEEFEYIKNH PIISYLMIAN LPDLDDNIKT LVLNHHRPHK GEGMNNNYPQ PKVLIHKLNV YKEKYKDDPK KTVLVADIQK QIRNILTNNL PMEDIGVISI AGEFASLTTR QAWREAFDPL VAMKLILNNS FFAYNEKTLR DFYDHIGLSL CNNQPFIREG DFVIVVTQDS NQKVFFEVCI IREMYKTQIR PMLERIGTIK PNFSNMGKLR ISGFDIASLK LDRRKAVYNL EKNQDPRRIV YVLDSNMDAR LYEELTKQTG EIPKESA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg cgcagaaaga cttacaaaaa ttcgatttta cagaagaaat catccagcat tttaaaatta acagcgtaat tcccgttgat ttttataata gaaacggaca gattttaatt cataaaaagg aaaatgcaga cggagatgac attacaaaat tattaagatt cgagagccag ggaatttact ttttaaaatc cgaattcgaa aagatttccg gcggaaaaca aggtgatggg ccaaataatg taaatggtcg agacgttagt tttgccaaac tagtcaatgc cgagttaacc gtagatcttg ctaagaacgc ttccaatttt ttatccgaat taaaaaaatt tccccttcat ggaaatcagt taagacatct aaataagtcc atagacggaa tattagaaga ttttaaatca actccagaca tggaaaccgg attggttaac atcatagaag ttatgagtag cgcaggggtt cctatggatt ccgaaatact tacaaaacgc accgtgattt caatggctat gaaagtgaga gccggaaaag cttttactaa ggtggacatg gaacagaaaa aattagatca aatgaatcta atgatgtctt cttatcttgc ggatgtgggc tacactcaga tgaaaattcc gatggagaga gatctgaaag cggaagaatt tgaatatatt aaaaaccatc caattattag ttatcttatg atagcaaatc ttccggactt ggacgataat attaaaactt tggttctcaa tcatcaccgt cctcataaag gggaaggaat gaataataac tatcctcaac caaaagttct tattcataag ctcaacgttt ataaggaaaa gtataaggac gatcctaaaa aaaccgtttt ggttgcagat attcaaaaac aaattcggaa tattcttacc aacaatcttc ctatggaaga tattggggtt atttccattg cgggagaatt tgcatccttg acgacaagac aagcgtggag agaagctttt gatcctttgg ttgcaatgaa attaattttg aataacagtt tttttgcata taatgaaaag accttgagag acttttacga tcatattggt ctttcattgt gtaataatca gccttttatt cgggaaggtg actttgtgat tgtggtcaca caagattcta atcaaaaagt tttttttgaa gtttgtatta ttcgagaaat gtataaaact caaattcgtc ctatgttaga aagaatagga accattaaac ctaactttag taatatgggt aaacttagaa tttcaggatt tgatattgct tctttaaagt tggatcgtag aaaagcggtt tacaatctcg aaaaaaacca agacccgaga aggatcgttt acgttttgga ttctaatatg gacgctcgtc tttatgagga attaactaaa caaaccggcg aaattcctaa agaaagtgct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LpinA.19564.a.B1.PS38499
PURIFICATION DATe: 9/5/2018
CONCENTRATION: 33.92mg/ml
OBSERVED MW: 57kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 12µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD AQKDLQKFDF TEEIIQHFKI NSVIPVDFYN RNGQILIHKK ENADGDDITK LLRFESQGIY FLKSEFEKIS GGKQGDGPNN VNGRDVSFAK LVNAELTVDL AKNASNFLSE LKKFPLHGNQ LRHLNKSIDG ILEDFKSTPD METGLVNIIE VMSSAGVPMD SEILTKRTVI SMAMKVRAGK AFTKVDMEQK KLDQMNLMMS SYLADVGYTQ MKIPMERDLK AEEFEYIKNH PIISYLMIAN LPDLDDNIKT LVLNHHRPHK GEGMNNNYPQ PKVLIHKLNV YKEKYKDDPK KTVLVADIQK QIRNILTNNL PMEDIGVISI AGEFASLTTR QAWREAFDPL VAMKLILNNS FFAYNEKTLR DFYDHIGLSL CNNQPFIREG DFVIVVTQDS NQKVFFEVCI IREMYKTQIR PMLERIGTIK PNFSNMGKLR ISGFDIASLK LDRRKAVYNL EKNQDPRRIV YVLDSNMDAR LYEELTKQTN EIPKESA
Validated NT Sequence
tgtttagtta attcctcata aagacgagcg tccatattag aatccaaaac gtaaacgatc cttctcgggt cttggttttt ttcgagattg taaaccgctt ttctacgatc caactttaaa gaagcaatat caaatcctga aattctaagt ttacccatat tactaaagtt aggtttaatg gttcctattc tttctaacat aggacgaatt tgagttttat acatttctcg aataatacaa acttcaaaaa aaactttttg attagaatct tgtgtgacca caatcacaaa gtcaccttcc cgaataaaag gctgattatt acacaatgaa agaccaatat gatcgtaaaa gtctctcaag gtcttttcat tatatgcaaa aaaactgtta ttcaaaatta atttcattgc aaccaaagga tcaaaagctt ctctccacgc ttgtcttgtc gtcaaggatg caaattctcc cgcaatggaa ataaccccaa tatcttccat aggaagattg ttggtaagaa tattccgaat ttgtttttga atatctgcaa ccaaaacggt ttttttagga tcgtccttat acttttcctt ataaacgttg agcttatgaa taagaacttt tggttgagga tagttattat tcattccttc ccctttatga ggacggtgat gattgagaac caaagtttta atattatcgt ccaagtccgg aagatttgct atcataagat aactaataat tggatggttt ttaatatatt caaattcttc cgctttcaga tctctctcca tcggaatttt catctgagtg tagcccacat ccgcaagata agaagacatc attagattca tttgatctaa ttttttctgt tccatgtcca ccttagtaaa agcttttccg gctctcactt tcatagccat tgaaatcacg gtgcgttttg taagtatttc ggaatccata ggaacccctg cgctactcat aacttctatg atgttaacca atccggtttc catgtctgga gttgatttaa aatcttctaa tattccgtct atggacttat ttagatgtct taactgattt ccatgaaggg gaaatttttt taattcggat aaaaaattgg aagcgttctt agcaagatct acggttaact cggcattgac tagtttggca aaactaacgt ctcgaccatt tacattattt ggcccatcac cttgttttcc gccggaaatc ttttcgaatt cggattttaa aaagtaaatt ccctggctct cgaatcttaa taattttgta atgtcatctc cgtctgcatt ttccttttta tgaattaaaa tctgtccgtt tctattataa aaatcaacgg gaattacgct gttaatttta aaatgctgga tgatttcttc tgtaaaatcg aatttttgta agtctttctg cgcgtccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMD AQKDLQKFDF TEEIIQHFKI NSVIPVDFYN RNGQILIHKK ENADGDDITK LLRFESQGIY FLKSEFEKIS GGKQGDGPNN VNGRDVSFAK LVNAELTVDL AKNASNFLSE LKKFPLHGNQ LRHLNKSIDG ILEDFKSTPD METGLVNIIE VMSSAGVPMD SEILTKRTVI SMAMKVRAGK AFTKVDMEQK KLDQMNLMMS SYLADVGYTQ MKIPMERDLK AEEFEYIKNH PIISYLMIAN LPDLDDNIKT LVLNHHRPHK GEGMNNNYPQ PKVLIHKLNV YKEKYKDDPK KTVLVADIQK QIRNILTNNL PMEDIGVISI AGEFASLTTR QAWREAFDPL VAMKLILNNS FFAYNEKTLR DFYDHIGLSL CNNQPFIREG DFVIVVTQDS NQKVFFEVCI IREMYKTQIR PMLERIGTIK PNFSNMGKLR ISGFDIASLK LDRRKAVYNL EKNQDPRRIV YVLDSNMDAR LYEELTKQTG EIPKESA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACG CGCAGAAAGA CTTACAAAAA TTCGATTTTA CAGAAGAAAT CATCCAGCAT TTTAAAATTA ACAGCGTAAT TCCCGTTGAT TTTTATAATA GAAACGGACA GATTTTAATT CATAAAAAGG AAAATGCAGA CGGAGATGAC ATTACAAAAT TATTAAGATT CGAGAGCCAG GGAATTTACT TTTTAAAATC CGAATTCGAA AAGATTTCCG GCGGAAAACA AGGTGATGGG CCAAATAATG TAAATGGTCG AGACGTTAGT TTTGCCAAAC TAGTCAATGC CGAGTTAACC GTAGATCTTG CTAAGAACGC TTCCAATTTT TTATCCGAAT TAAAAAAATT TCCCCTTCAT GGAAATCAGT TAAGACATCT AAATAAGTCC ATAGACGGAA TATTAGAAGA TTTTAAATCA ACTCCAGACA TGGAAACCGG ATTGGTTAAC ATCATAGAAG TTATGAGTAG CGCAGGGGTT CCTATGGATT CCGAAATACT TACAAAACGC ACCGTGATTT CAATGGCTAT GAAAGTGAGA GCCGGAAAAG CTTTTACTAA GGTGGACATG GAACAGAAAA AATTAGATCA AATGAATCTA ATGATGTCTT CTTATCTTGC GGATGTGGGC TACACTCAGA TGAAAATTCC GATGGAGAGA GATCTGAAAG CGGAAGAATT TGAATATATT AAAAACCATC CAATTATTAG TTATCTTATG ATAGCAAATC TTCCGGACTT GGACGATAAT ATTAAAACTT TGGTTCTCAA TCATCACCGT CCTCATAAAG GGGAAGGAAT GAATAATAAC TATCCTCAAC CAAAAGTTCT TATTCATAAG CTCAACGTTT ATAAGGAAAA GTATAAGGAC GATCCTAAAA AAACCGTTTT GGTTGCAGAT ATTCAAAAAC AAATTCGGAA TATTCTTACC AACAATCTTC CTATGGAAGA TATTGGGGTT ATTTCCATTG CGGGAGAATT TGCATCCTTG ACGACAAGAC AAGCGTGGAG AGAAGCTTTT GATCCTTTGG TTGCAATGAA ATTAATTTTG AATAACAGTT TTTTTGCATA TAATGAAAAG ACCTTGAGAG ACTTTTACGA TCATATTGGT CTTTCATTGT GTAATAATCA GCCTTTTATT CGGGAAGGTG ACTTTGTGAT TGTGGTCACA CAAGATTCTA ATCAAAAAGT TTTTTTTGAA GTTTGTATTA TTCGAGAAAT GTATAAAACT CAAATTCGTC CTATGTTAGA AAGAATAGGA ACCATTAAAC CTAACTTTAG TAATATGGGT AAACTTAGAA TTTCAGGATT TGATATTGCT TCTTTAAAGT TGGATCGTAG AAAAGCGGTT TACAATCTCG AAAAAAACCA AGACCCGAGA AGGATCGTTT ACGTTTTGGA TTCTAATATG GACGCTCGTC TTTATGAGGA ATTAACTAAA CAAACCGGCG AAATTCCTAA AGAAAGTGCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT