LpinA.19560.a

Hemolysin

CENTER ID: LpinA.19560.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19560.a.B2.GE41962 mature protein 25 623
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.3156
UniProt: Q72P45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINKITKPKL LIGYYLLLFS LIRCLPEKES SYKDLFTSLL FLPNQTNSNQ VNSVSINNDP ANPNPVNPAS ANNNQVNAVP ENDNPANLNP VNPASANSNQ VNAAPENGSS ADPNPANLAS GNNNQVNAVP ANNYFTKEDS SNNIPKKVNS KNVEIKVLSH NVFMLPTNLP RWGNLGHDER AKRISKSDYV KNQDVIVFEE AFDTSARKIL LDNLREEYPY QTDVVGRTKK NWDASLGNFR SYSLVNGGVV ILSKWPIEEK IQYIFNDSGC GTDWFANKGF VYVKINKEGK KFHVIGTHAQ SQDQNCSNLG IPNRANQFDD IRNFIYSKNI PKDETVLIVG DLNVIKESNE YYDMISRLNV NEPRYVGVPF TWDAKTNEIA AYYYENEEPV YLDYIFVSKS HAQPPVWQNL AYDPVSKQTW TVSGYTSDEF SDHYPIYGFV YADPSTPTKS GHKKKYDQVS FQSAANGKYI QADPNRKNGW LKADAVIETD FTKFNLLQEG NLNPSCIKNG LVRIESSRFL NYFWNWWLGG GSGNYGYYSK FNDASNQLEI INLSDGCLEN GSKIVFKDYD TYSRNHYYLT VWDKGNWNEH LYLWKDSISQ REIFYLKLNS TPVRNWSADL IYR
NT Sequence
atgataaaca aaataacaaa accaaaacta cttataggtt attacctatt gttattctct ctgatacgtt gtttacccga aaaagaatcc tcatataagg atttatttac ttcgttatta ttcctcccca accaaacaaa tagtaatcaa gtaaattcgg tttccataaa caacgaccct gcaaacccaa atccagttaa cccggcatcc gcaaataaca atcaggtaaa cgcagttcca gaaaatgaca atccagcaaa cctaaatcca gttaatccgg catctgcaaa tagcaatcaa gtaaatgcag ctccagaaaa tggcagctcc gcagatccaa atccagccaa cctcgcatct ggaaataata atcaagtaaa tgcagttcca gctaacaatt attttacgaa agaagattcc tcaaataata ttcctaaaaa agtaaattct aaaaatgtag aaataaaagt actgagtcac aacgtattta tgttgcccac aaatcttcca cgatggggaa atttgggaca cgatgaaaga gcaaaacgaa tttcaaaatc agattacgta aaaaatcagg acgttatcgt gttcgaagaa gccttcgaca caagtgcaag aaaaattctt ttagacaatc ttcgagaaga atatccgtat caaacagatg tggtcggaag aacaaaaaaa aattgggacg caagcttagg caattttaga tcttattcgc tcgttaacgg aggagttgta atcctaagta aatggcctat cgaagaaaaa attcaatata tctttaacga ctcaggttgt ggaacggatt ggtttgcaaa taaaggattt gtttatgtaa aaatcaacaa agaaggaaaa aaattccatg tcatcggaac ccacgctcag tctcaggatc agaactgttc aaatctagga ataccaaaca gagctaatca atttgatgac attagaaatt tcatatattc taaaaatatt ccaaaagatg aaaccgtttt aatcgtgggc gatttaaacg ttatcaaaga aagtaacgaa tattacgata tgatttctag gttaaatgtt aacgaaccta gatatgttgg agttcctttt acttgggatg caaaaacaaa cgaaattgct gcatactatt acgaaaacga agaaccggtt tacttagatt atattttcgt ttcaaaatca cacgctcaac caccggtttg gcaaaattta gcttacgatc cggtttctaa acaaacttgg accgtatctg gatatacaag cgacgaattt tcggatcact atccgatata cggttttgta tacgcggatc cttctactcc tacaaagtcc ggacataaaa aaaaatacga tcaagtctct tttcaatcag ctgctaatgg taaatacatt caagcagatc ctaatagaaa aaacggatgg ttaaaagcgg acgctgtaat agaaacggat tttactaaat tcaacctatt gcaagaagga aatttaaatc cttcctgcat aaaaaatggt ctcgttagaa ttgaatcttc ccgtttttta aactactttt ggaactggtg gttaggcgga ggttccggta actacggcta ttactctaag tttaatgacg cttccaatca acttgaaatt atcaatttga gtgatggatg cctagaaaac ggtagtaaaa tcgtattcaa agattatgat acttattcta gaaaccatta ctatctcacc gtttgggaca aaggaaattg gaatgaacat ctttatcttt ggaaagattc gatcagccag agagaaatct tttatctcaa attaaattct actccggtaa gaaattggag tgcggatctt atttatcgct aa
Details for LpinA.19560.a.B2.GE41962
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML PEKESSYKDL FTSLLFLPNQ TNSNQVNSVS INNDPANPNP VNPASANNNQ VNAVPENDNP ANLNPVNPAS ANSNQVNAAP ENGSSADPNP ANLASGNNNQ VNAVPANNYF TKEDSSNNIP KKVNSKNVEI KVLSHNVFML PTNLPRWGNL GHDERAKRIS KSDYVKNQDV IVFEEAFDTS ARKILLDNLR EEYPYQTDVV GRTKKNWDAS LGNFRSYSLV NGGVVILSKW PIEEKIQYIF NDSGCGTDWF ANKGFVYVKI NKEGKKFHVI GTHAQSQDQN CSNLGIPNRA NXFDDIRNFI YSKNIPKDET VLIVGDLNVI KESNEYYDMI SRLNVNEPRY VGVPFTWDAK TNEIAAYYYE NEEPVYLDYI FVSKSHAQPP VWQNLAYDPV SKQTWTVSGY TSDEFSDHYP IYGFVYADPS TPTKSGHKKK YDQVSFQSAA NGKYIQADPN RKNGWLKADA VIETDFTKFN LLQEGNLNPS CIKNGLVRIE SSRFLNYFWN WWLGGGSGNY GYYSKFNDAS NQLEIINLSD GCLENGSKIV FKDYDTYSRN HYYLTVWDKG NWNEHLYLWK DSISQREIFY LKLNSTPVRN WSADLIYR
Validated NT Sequence
atggctcacc accaccacca ccatatgtta cccgaaaaag aatcctcata taaggattta tttacttcgt tattattcct ccccaaccaa acaaatagta atcaagtaaa ttcggtttcc ataaacaacg accctgcaaa cccaaatcca gttaacccgg catccgcaaa taacaatcag gtaaacgcag ttccagaaaa tgacaatcca gcaaacctaa atccagttaa tccggcatct gcaaatagca atcaagtaaa tgcagctcca gaaaatggca gctccgcaga tccaaatcca gccaacctcg catctggaaa taataatcaa gtaaatgcag ttccagctaa caattatttt acgaaagaag attcctcaaa taatattcct aaaaaagtaa attctaaaaa tgtagaaata aaagtactga gtcacaacgt atttatgttg cccacaaatc ttccacgatg gggaaatttg ggacacgatg aaagagcaaa acgaatttca aaatcagatt acgtaaaaaa tcaggacgtt atcgtgttcg aagaagcctt cgacacaagt gcaagaaaaa ttcttttaga caatcttcga gaagaatatc cgtatcaaac agatgtggtc ggaagaacaa aaaaaaattg ggacgcaagc ttaggcaatt ttagatctta ttcgctcgtt aacggaggag ttgtaatcct aagtaaatgg cctatcgaag aaaaaattca atatatcttt aacgactcag gttgtggaac ggattgg-tt tgcaaataaa ggatttgttt atgtaaaaat caacaaagaa ggaaaaaaat tccatgtcat cggaacccac gctcagtctc aggatcagaa ctgttcaaat ctaggaatac cnaacagagc taatcanttt gatgacatta gaaatttcat a-tattctaa aaatattcca aaagatgaaa ccgttttaat cgtggg-cga tttaaacgtt atcaaagaaa gtaacgaata ttacgatatg atttctaggt taaatgttaa cgaacctaga tatgttggag ttccttttac ttgggatgca aaaacaaacg aaattgctgc atactattac gaaaacgaag aaccggttta cttagattat attttcgttt caaaatcaca cgctcaacca ccggtttggc aaaatttagc ttacgatccg gtttctaaac aaacttggac cgtatctgga tatacaagcg acgaattttc ggatcactat ccgatatacg gttttgtata cgcggatcct tctactccta caaagtccgg acataaaaaa aaatacgatc aagtctcttt tcaatcagct gctaatggta aatacattca agcagatcct aatagaaaaa acggatggtt aaaagcggac gctgtaatag aaacggattt tactaaattc aacctattgc aagaaggaaa tttaaatcct tcctgcataa aaaatggtct cgttagaatt gaatcttccc gttttttaaa ctacttttgg aactggtggt taggcggagg ttccggtaac tacggctatt actctaagtt taatgacgct tccaatcaac ttgaaattat caatttgagt gatggatgcc tagaaaacgg tagtaaaatc gtattcaaag attatgatac ttattctaga aaccattact atctcaccgt ttgggacaaa ggaaattgga atgaacatct ttatctttgg aaagattcga tcagccagag agaaatcttt tatctcaaat taaattctac tccggtaaga aattggagtg cggatcttat ttatcgctaa gtgagtaaga taggatccgg ctgc
Expected Protein Sequence
MAHHHHHHLP EKESSYKDLF TSLLFLPNQT NSNQVNSVSI NNDPANPNPV NPASANNNQV NAVPENDNPA NLNPVNPASA NSNQVNAAPE NGSSADPNPA NLASGNNNQV NAVPANNYFT KEDSSNNIPK KVNSKNVEIK VLSHNVFMLP TNLPRWGNLG HDERAKRISK SDYVKNQDVI VFEEAFDTSA RKILLDNLRE EYPYQTDVVG RTKKNWDASL GNFRSYSLVN GGVVILSKWP IEEKIQYIFN DSGCGTDWFA NKGFVYVKIN KEGKKFHVIG THAQSQDQNC SNLGIPNRAN QFDDIRNFIY SKNIPKDETV LIVGDLNVIK ESNEYYDMIS RLNVNEPRYV GVPFTWDAKT NEIAAYYYEN EEPVYLDYIF VSKSHAQPPV WQNLAYDPVS KQTWTVSGYT SDEFSDHYPI YGFVYADPST PTKSGHKKKY DQVSFQSAAN GKYIQADPNR KNGWLKADAV IETDFTKFNL LQEGNLNPSC IKNGLVRIES SRFLNYFWNW WLGGGSGNYG YYSKFNDASN QLEIINLSDG CLENGSKIVF KDYDTYSRNH YYLTVWDKGN WNEHLYLWKD SISQREIFYL KLNSTPVRNW SADLIYR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttacccg aaaaagaatc ctcatataag gatttattta cttcgttatt attcctcccc aaccaaacaa atagtaatca agtaaattcg gtttccataa acaacgaccc tgcaaaccca aatccagtta acccggcatc cgcaaataac aatcaggtaa acgcagttcc agaaaatgac aatccagcaa acctaaatcc agttaatccg gcatctgcaa atagcaatca agtaaatgca gctccagaaa atggcagctc cgcagatcca aatccagcca acctcgcatc tggaaataat aatcaagtaa atgcagttcc agctaacaat tattttacga aagaagattc ctcaaataat attcctaaaa aagtaaattc taaaaatgta gaaataaaag tactgagtca caacgtattt atgttgccca caaatcttcc acgatgggga aatttgggac acgatgaaag agcaaaacga atttcaaaat cagattacgt aaaaaatcag gacgttatcg tgttcgaaga agccttcgac acaagtgcaa gaaaaattct tttagacaat cttcgagaag aatatccgta tcaaacagat gtggtcggaa gaacaaaaaa aaattgggac gcaagcttag gcaattttag atcttattcg ctcgttaacg gaggagttgt aatcctaagt aaatggccta tcgaagaaaa aattcaatat atctttaacg actcaggttg tggaacggat tggtttgcaa ataaaggatt tgtttatgta aaaatcaaca aagaaggaaa aaaattccat gtcatcggaa cccacgctca gtctcaggat cagaactgtt caaatctagg aataccaaac agagctaatc aatttgatga cattagaaat ttcatatatt ctaaaaatat tccaaaagat gaaaccgttt taatcgtggg cgatttaaac gttatcaaag aaagtaacga atattacgat atgatttcta ggttaaatgt taacgaacct agatatgttg gagttccttt tacttgggat gcaaaaacaa acgaaattgc tgcatactat tacgaaaacg aagaaccggt ttacttagat tatattttcg tttcaaaatc acacgctcaa ccaccggttt ggcaaaattt agcttacgat ccggtttcta aacaaacttg gaccgtatct ggatatacaa gcgacgaatt ttcggatcac tatccgatat acggttttgt atacgcggat ccttctactc ctacaaagtc cggacataaa aaaaaatacg atcaagtctc ttttcaatca gctgctaatg gtaaatacat tcaagcagat cctaatagaa aaaacggatg gttaaaagcg gacgctgtaa tagaaacgga ttttactaaa ttcaacctat tgcaagaagg aaatttaaat ccttcctgca taaaaaatgg tctcgttaga attgaatctt cccgtttttt aaactacttt tggaactggt ggttaggcgg aggttccggt aactacggct attactctaa gtttaatgac gcttccaatc aacttgaaat tatcaatttg agtgatggat gcctagaaaa cggtagtaaa atcgtattca aagattatga tacttattct agaaaccatt actatctcac cgtttgggac aaaggaaatt ggaatgaaca tctttatctt tggaaagatt cgatcagcca gagagaaatc ttttatctca aattaaattc tactccggta agaaattgga gtgcggatct tatttatcgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat