SSGCID: Target Page

LpinA.19558.a

Uncharacterized protein

CENTER ID:	LpinA.19558.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19558.a.B2.GE42904	mature protein		18	304

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.3998
UniProt:	Q72M87

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLFLYFIFSA CISALVAHPC ATEFKIDTVE KEGVGSIQLT PTNPNVSVIQ PQFDKVGQFS ESLAPVRIGN KWGYIDYTGT FVILPQFDDA KPFSEGLAAV KVKNKWGYID PTGKIVIQPR FDETKFFSQG LAAVKVGGKW GYVDKTGKIA IRLRFNDADS FSEDLAVVEI KGEEYGYINK SGKIVIRPQF ENVYSYSLGS FSKGFAEVRV SYFIRVRTFY INKLGERQIP GFIPPNHYEL EKIVVFRNEN KWGFFSTKGE PEIISPHFDA AHSPTDGLAP VRVENKWGYI DFTKCLESGD ISEK

NT Sequence

atgctatttt tgtatttcat attttctgca tgtatttccg cacttgtagc acatccgtgt gccaccgaat ttaaaataga cacggttgag aaggaaggag tcggctctat tcaactaacg cctacaaatc cgaatgtttc cgtgattcag cctcaattcg ataaggtcgg gcaattttca gaaagccttg cacccgtacg gattggaaac aaatggggat atattgatta taccggaacg tttgtaattc tacctcaatt tgatgatgcg aaaccttttt cagaaggtct tgcggccgtt aaggttaaaa ataaatgggg gtatattgat cctacgggaa aaatcgtgat tcaacctcgg ttcgatgaaa cgaaattttt ttcacaagga cttgctgctg tgaaagttgg aggtaaatgg ggatatgttg ataaaaccgg aaaaatcgcc attcggcttc gatttaacga tgcggattcc ttttcggagg accttgcggt cgtagaaatt aagggagaag aatatggata tatcaataag tctggaaaaa ttgtcattcg acctcaattt gaaaacgtat attcatattc tttaggctct ttttcgaaag gttttgccga agtgagggtg agttatttca tacgggtgcg aacgttttat attaataaat taggcgaacg ccaaatacca ggttttattc ctcccaatca ttacgaatta gaaaaaatcg tagtttttcg taatgagaat aaatgggggt ttttcagcac aaaaggggag ccggagataa tttcaccaca cttcgatgca gcacattcac ctacagatgg tcttgcgcca gtccgtgtcg aaaacaaatg gggatatatc gattttacga aatgtttgga aagcggcgac atatctgaaa aataa

Details for LpinA.19558.a.B2.GE42904

HARVESTED ON:	2/7/2019
SEQUENCED ON:	2/8/2019
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHHP CATEFKIDTV EKEGVGSIQL TPTNPNVSVI QPQFDKVGQF SESLAPVRIG NKWGYIDYTG TFVILPQFDD AKPFSEGLAA VKVKNKWGYI DPTGKIVIQP RFDETKFFSQ GLAAVKVGGK WGYVDKTGKI AIRLRFNDAD SFSEDLAVVE IKGEEYGYIN KSGKIVIRPQ FENVYSYSLG SFSKGFAEVR VSYFIRVRTF YINKLGERQI PGFIPPNHYE LEKIVVFRNE NKWGFFSTKG EPEIISPHFD AAHSPTDGLA PVRVENKWGY IDFTKCLESG DISEK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcatccgt gtgccaccga atttaaaata gacacggttg agaaggaagg agtcggctct attcaactaa cgcctacaaa tccgaatgtt tccgtgattc agcctcaatt cgataaggtc gggcaatttt cagaaagcct tgcacccgta cggattggaa acaaatgggg atatattgat tataccggaa cgtttgtaat tctacctcaa tttgatgatg cgaaaccttt ttcagaaggt cttgcggccg ttaaggttaa aaataaatgg gggtatattg atcctacggg aaaaatcgtg attcaacctc ggttcgatga aacgaaattt ttttcacaag gacttgctgc tgtgaaagtt ggaggtaaat ggggatatgt tgataaaacc ggaaaaatcg ccattcggct tcgatttaac gatgcggatt ccttttcgga ggaccttgcg gtcgtagaaa ttaagggaga agaatatgga tatatcaata agtctggaaa aattgtcatt cgacctcaat ttgaaaacgt atattcatat tctttaggct ctttttcgaa aggttttgcc gaagtgaggg tgagttattt catacgggtg cgaacgtttt atattaataa attaggcgaa cgccaaatac caggttttat tcctcccaat cattacgaat tagaaaaaat cgtagttttt cgtaatgaga ataaatgggg gtttttcagc acaaaagggg agccggagat aatttcacca cacttcgatg cagcacattc acctacagat ggtcttgcgc cagtccgtgt cgaaaacaaa tggggatata tcgattttac gaaatgtttg gaaagcggcg acatatctga aaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat