LpinA.19556.a

Putative lipoprotein

CENTER ID: LpinA.19556.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19556.a.B2.GE41961 mature protein 25 421
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.1548
UniProt: Q72SW8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKQCRNVKIM TLLSSALILF VSCAKHKDNK NDLLLAAILL NGGTNAEFRI SNINALVAAR TAANSSQSRI GESNASAFLT DLGGDNPQNY GDGAADGFND HFITPAAISI GVCQVVAYKS VAKGGPAKGS ETLENANFIM FELSGSNVVP GAKMCGSGFM PIGLQTGDST LSSAFLPITP FPEAEKQDYD RVGIIARDFT YYFDPKDVPE NSYRYVDLVL NNPISNIESN RTDDGITRGD VSPKLFNKNC PESFLNSPSY IYSDLLKSGE IENISGSCMM GELAIDANSG HILRTAVPDN LNPFTDPTNM LNPPSVAGVA FASATQKLKF KTPTSINNLD IKDPYILVVD LDSSKKDGGS LLFNVSIDKV LFWDSNSADN VFSPQLDAGD SPNATSGNDN LTNTARKNMI FHLPTIISNY K
NT Sequence
atgaaacaat gtaggaacgt taagatcatg actcttttat cgtcagcctt aattttattt gtaagttgtg cgaaacataa agacaataag aatgatttat tgttagcggc aattttgctt aatggtggaa caaacgccga gttccgcatt tccaatatca atgcccttgt agcagcgcgc actgcggcaa attcatccca atctagaatt ggagaaagta acgcttctgc attcctaacg gatcttggcg gagacaaccc tcaaaattat ggagacggtg cagcggatgg ttttaacgat cattttatca cgccagctgc aatttctatc ggagtatgcc aagttgttgc ttataaatca gtagcaaaag gtgggccggc aaaaggaagt gagacattag aaaatgcgaa ttttataatg tttgaattat cgggatcaaa tgttgtccct ggcgcaaaaa tgtgtggaag tggctttatg cccatcggat tacaaaccgg agacagtact ttaagttcgg cttttcttcc aattactcca tttccagaag cggaaaaaca agactatgat cgagtaggaa tcatcgccag agactttact tattattttg acccgaaaga tgtccctgaa aattcatacc gttatgtgga cttggtttta aataatccta tttctaatat tgagtctaac cgaaccgacg atggaattac cagaggcgac gtaagtccaa aactttttaa taaaaattgt ccggaatctt ttttgaactc tccaagttat atctactccg atttgttaaa atcgggagaa atagaaaata tctcaggtag ttgtatgatg ggcgaattag cgatcgacgc aaattcaggt catatactta gaactgcagt tccagacaat cttaaccctt ttacagatcc tacaaacatg ctcaatccac catctgtggc aggcgttgca tttgcttccg ccacacaaaa actaaaattc aaaacgccga cttccatcaa taatttggac ataaaagatc catacatatt ggttgtagat ctggactctt ctaaaaaaga cgggggaagt cttttattta acgtttccat tgataaagtt cttttttggg actcaaactc ggcggacaac gtcttctctc ctcagctcga tgctggcgat agtcctaacg caacaagtgg aaacgataat ttaacaaata cagctaggaa aaatatgatc ttccacttac caacgatcat cagcaactat aagtaa
Details for LpinA.19556.a.B2.GE41961
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK HKDNKNDLLL AAILLNGGTN AEFRISNINA LVAARTAANS SQSRIGESNA SAFLTDLGGD NPQNYGDGAA DGFNDHFITP AAISIGVCQV VAYKSVAKGG PAKGSETLEN ANFIMFELSG SNVVPGAKMC GSGFMPIGLQ TGDSTLSSAF LPITPFPEAE KQDYDRVGII ARDFTYYFDP KDVPENSYRY VDLVLNNPIS NIESNRTDDG ITRGDVSPKL FNKNCPESFL NSPSYIYSDL LKSGEIENIS GSCMMGELAI DANSGHILRT AVPDNLNPFT DPTNMLNPPS VAGVAFASAT QKLKFKTPTS INNLDIKDPY ILVVDLDSSK KDGGSLLFNV SIDKVLFWDS NSADNVFSPQ LDAGDSPNAT SGNDNLTNTA RKNMIFH
Validated NT Sequence
aagtggaaga tcatattttt cctagctgta tttgttaaat tatcgtttcc acttgttgcg ttaggactat cgccagcatc gagctgagga gagaagacgt tgtccgccga gtttgagtcc caaaaaagaa ctttatcaat ggaaacgtta aataaaagac ttcccccgtc ttttttagaa gagtccagat ctacaaccaa tatgtatgga tcttttatgt ccaaattatt gatggaagtc ggcgttttga attttagttt ttgtgtggcg gaagcaaatg caacgcctgc cacagatggt ggattgagca tgtttgtagg atctgtaaaa gggttaagat tgtctggaac tgcagttcta agtatatgac ctgaatttgc gtcgatcgct aattcgccca tcatacaact acctgagata ttttctattt ctcccgattt taacaaatcg gagtagatat aacttggaga gttcaaaaaa gattccggac aatttttatt aaaaagtttt ggacttacgt cgcctctggt aattccatcg tcggttcggt tagactcaat attagaaata ggattattta aaaccaagtc cacataacgg tatgaatttt cagggacatc tttcgggtca aaataataag taaagtctct ggcgatgatt cctactcgat catagtcttg tttttccgct tctggaaatg gagtaattgg aagaaaagcc gaacttaaag tactgtctcc ggtttgtaat ccgatgggca taaagccact tccacacatt tttgcgccag ggacaacatt tgatcccgat aattcaaaca ttataaaatt cgcattttct aatgtctcac ttccttttgc cggcccacct tttgctactg atttataagc aacaacttgg catactccga tagaaattgc agctggcgtg ataaaatgat cgttaaaacc atccgctgca ccgtctccat aattttgagg gttgtctccg ccaagatccg ttaggaatgc agaagcgtta ctttctccaa ttctagattg ggatgaattt gccgcagtgc gcgctgctac aagggcattg atattggaaa tgcggaactc ggcgtttgtt ccaccattaa gcaaaattgc cgctaacaat aaatcattct tattgtcttt atgtttcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHKH KDNKNDLLLA AILLNGGTNA EFRISNINAL VAARTAANSS QSRIGESNAS AFLTDLGGDN PQNYGDGAAD GFNDHFITPA AISIGVCQVV AYKSVAKGGP AKGSETLENA NFIMFELSGS NVVPGAKMCG SGFMPIGLQT GDSTLSSAFL PITPFPEAEK QDYDRVGIIA RDFTYYFDPK DVPENSYRYV DLVLNNPISN IESNRTDDGI TRGDVSPKLF NKNCPESFLN SPSYIYSDLL KSGEIENISG SCMMGELAID ANSGHILRTA VPDNLNPFTD PTNMLNPPSV AGVAFASATQ KLKFKTPTSI NNLDIKDPYI LVVDLDSSKK DGGSLLFNVS IDKVLFWDSN SADNVFSPQL DAGDSPNATS GNDNLTNTAR KNMIFHLPTI ISNYK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaacata aagacaataa gaatgattta ttgttagcgg caattttgct taatggtgga acaaacgccg agttccgcat ttccaatatc aatgcccttg tagcagcgcg cactgcggca aattcatccc aatctagaat tggagaaagt aacgcttctg cattcctaac ggatcttggc ggagacaacc ctcaaaatta tggagacggt gcagcggatg gttttaacga tcattttatc acgccagctg caatttctat cggagtatgc caagttgttg cttataaatc agtagcaaaa ggtgggccgg caaaaggaag tgagacatta gaaaatgcga attttataat gtttgaatta tcgggatcaa atgttgtccc tggcgcaaaa atgtgtggaa gtggctttat gcccatcgga ttacaaaccg gagacagtac tttaagttcg gcttttcttc caattactcc atttccagaa gcggaaaaac aagactatga tcgagtagga atcatcgcca gagactttac ttattatttt gacccgaaag atgtccctga aaattcatac cgttatgtgg acttggtttt aaataatcct atttctaata ttgagtctaa ccgaaccgac gatggaatta ccagaggcga cgtaagtcca aaacttttta ataaaaattg tccggaatct tttttgaact ctccaagtta tatctactcc gatttgttaa aatcgggaga aatagaaaat atctcaggta gttgtatgat gggcgaatta gcgatcgacg caaattcagg tcatatactt agaactgcag ttccagacaa tcttaaccct tttacagatc ctacaaacat gctcaatcca ccatctgtgg caggcgttgc atttgcttcc gccacacaaa aactaaaatt caaaacgccg acttccatca ataatttgga cataaaagat ccatacatat tggttgtaga tctggactct tctaaaaaag acgggggaag tcttttattt aacgtttcca ttgataaagt tcttttttgg gactcaaact cggcggacaa cgtcttctct cctcagctcg atgctggcga tagtcctaac gcaacaagtg gaaacgataa tttaacaaat acagctagga aaaatatgat cttccactta ccaacgatca tcagcaacta taagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat