LpinA.19553.b

Thermolysin

CENTER ID: LpinA.19553.b
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19553.b.B2.GE42903 mature protein 31 794
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.4024
UniProt: Q72M68

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLLKLLKIN SNIKILLSMF MISILFSCKD KQDPGMESWL WVLNPVQLDN KALSERTYTS DGRITFAKFN SDLVPYSRKQ APEVLKTYLQ LPLTSQATFL RSARVGDFDH DRFQQRYKGV RVENGIYTVV SKENTIELMM GEFYQVPENF NSSPKLSESE ALSKTLKHIG AKKYIWESVE REAALKRKKN DPNASYFPKG ELLVYEHSLG KSNTKKEFRL AYKFGIASID PPISRYVYVD SNSGEILSSK DARRYEAGGG DTIPTTPPTP PDTNYGICFP DQTPCIKQGS ASTRFSGVKT ITTYTAGEEN HYELKDYSRG KGIITYSWEF IEDPFMGIYL LNVPIVDINN GWTKFEYHDD YNHDALLDAH WGIEMTYDYF KSVHNRLSYD GNDSKVVNNV HYFNIFVGNN AYWDPMTEEI YYIYCPHNSS TCKSLNLPII LDPTYEDFTS LDIVSHEFGH GINGDLAGFT YDPEPGALDE GFSDIWNVGV NNYVNKVLGM QKNIWLVGDE TVPGGGMRSV SNPKSTTVMS PGPNTYYGGL WDSENNEAHT NSLVLSHWFY TLSKGKQGIN DHWCEYNVSG INIEKAEKIA YAALYYLFPT SGYTSARTAA IYSAKALYGK FSSEVKSTID AWDAIGVPAD TTSRGGQGMY KPHYYITFVK LSNLERSSGN DCGYKDNSYL HPTVIKGFTY NMVLSSEGEV SIPSKVHKWR VWIDFNRNGS FESSEMVVQD SINDTFGGTL QKSIQIPTSA LTGDTRMRVS MKAVQSGESY QLANESFTEG EVEDYSITIN NFSI
NT Sequence
atgagacttt taaagttatt aaagatcaat tcgaatataa agatactctt aagtatgttt atgatttcga tattattttc ttgtaaggat aagcaggacc caggaatgga atcttggttg tgggttttaa atcctgtcca attggataat aaggcgcttt ctgaaaggac gtatacgtca gatggtagga taacatttgc gaagttcaat tcggatctag ttccttattc acgtaaacaa gcacctgaag ttcttaagac gtatttacaa ttacccttaa cttctcaagc tacattcttg cgttcggctc gtgtaggaga ttttgatcac gatcgttttc aacagagata taaaggtgtt agagtagaga atggaattta tacagtggtc tcaaaagaga atacgataga actgatgatg ggtgaatttt accaagttcc agagaatttt aattcctctc ctaaactatc cgagtcagaa gcactttcaa agactttaaa acatatcgga gctaagaagt atatttggga atctgttgaa cgagaagcag cattgaaacg taagaaaaat gatccaaatg caagttattt tccgaaggga gaactattag tttatgaaca ttcattagga aaatccaata ctaaaaaaga gtttcgattg gcttataaat ttggtatagc ctcgatagat ccaccaatat cgagatacgt gtatgtggat tctaactctg gggaaattct atccagtaaa gatgctagaa gatacgaagc gggtggagga gatacaattc caacgactcc accaactcct ccggatacaa attacggaat ttgttttccg gatcaaactc cttgtatcaa acaaggatca gcgtctactc gttttagcgg agtgaaaacg attacaactt atactgcagg agaagaaaat cattatgaac tgaaggatta ttcgagaggt aaaggaatca ttacgtattc ttgggaattt atagaagatc catttatggg tatttattta ttgaatgttc ccattgttga tattaataac ggttggacga aatttgaata tcatgacgat tataatcatg atgcattatt ggatgcacat tggggaattg aaatgacgta tgattatttc aaatccgttc ataatcggtt gagttatgat ggaaatgata gcaaagtagt gaataacgtg cactatttta atattttcgt aggtaataat gcatactggg atcctatgac agaggaaatc tattatatat actgtcctca taacagtagt acatgcaaaa gtcttaattt acctattatt ttagacccta catatgaaga ttttacttct ttggatattg tatctcatga atttggacat ggtatcaacg gggatcttgc aggttttact tatgatccag agccaggtgc tcttgatgaa ggttttagtg atatttggaa tgttggtgta aataactacg taaataaagt tttaggaatg caaaagaaca tttggttagt aggagatgaa acggttccgg gaggaggaat gcgttctgtg tcaaatccaa aatctactac agtaatgtcc cctgggccaa atacgtatta tggaggtctt tgggattctg aaaataacga agcgcatacg aatagtcttg ttttgagtca ttggttctat actttatcca agggaaaaca agggatcaat gatcattggt gtgagtataa cgtttctgga atcaacattg aaaaggcgga aaaaatcgct tatgctgctc tctattatct tttccctaca tccggttata cttctgcaag gaccgctgcg atttattcgg ctaaggcttt gtatggtaag ttttcatcgg aagtaaaaag tacaattgat gcttgggatg caataggtgt tccagctgat acaacttctc gcggtggaca aggaatgtat aagccgcact attatatcac ttttgtaaaa ctttctaatt tagagagaag ttccggaaat gattgtgggt acaaggataa ttcataccta catcctactg tcattaaagg atttacgtat aacatggtgt tatcgagcga aggtgaggta agtattccgt ctaaggttca caaatggaga gtatggatcg atttcaatcg aaatggtagt tttgaatcct cggaaatggt agttcaggat tctatcaacg atacatttgg aggaacactt caaaaatcga ttcagattcc aacgagtgcg cttactggcg atacgaggat gagagtttct atgaaggcag tccaaagcgg agaaagttat caacttgcga atgaatcctt taccgaagga gaagttgaag attattcgat aactatcaat aatttcagta tttag
Details for LpinA.19553.b.B2.GE42903
HARVESTED ON: 2/7/2019
SEQUENCED ON: 2/8/2019
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHKQ DPGMESWLWV LNPVQLDNKA LSERTYTSDG RITFAKFNSD LVPYSRKQAP EVLKTYLQLP LTSQATFLRS ARVGDFDHDR FQQRYKGVRV ENGIYTVVSK ENTIELMMGE FYQVPENFNS SPKLSESEAL SKTLKHIGAK KYIWESVERE AALKRKKNDP NASYFPKGEL LVYEHSLGKS NTKKEFRLAY KFGIASIDPP ISRYVYVDSN SGEILSSKDA RRYEAGGGDT IPTTPPTPPD TNYGICFPDQ TPCIKQGSAS TRFSGVKTIT TYTAGEENHY ELKDYSRGKG IITYSWEFIE DPFMGIYLLN VPIVDINNGW TKFEYHDDYN HDALLDAHWG IEMTYDYFKS VHNRLSYDGN DSKVVNNVHY FNIFVGNNAY WDPMTEEIYY IYCPHNSSTC KSLNLPIILD PTYEDFTSLD IVSHEFGHGI NGDLAGFTYD PEPGALDEGF SDIWNVGVNN YVNKVLGMQK NIWLVGDETV PGGGMRSVSN PKSTTVMSPG PNTYYGGLWD SENNEAHTNS LVLSHWFYTL SKGKQGINDH WCEYNVSGIN IEKAEKIAYA ALYYLFPTSG YTSARTAAIY SAKALYGKFS SEVKSTIDAW DAIGVPADTT SRGGQGMYKP HYYITFVKLS NLERSSGNDC GYKDNSYLHP TVIKGFTYNM VLSSEGEVSI PSKVHKWRVW IDFNRNGSFE SSEMVVQDSI NDTFGGTLQK SIQIPTSALT GDTRMRVSMK AVQSGESYQL ANESFTEGEV EDYSITINNF SI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagcagg acccaggaat ggaatcttgg ttgtgggttt taaatcctgt ccaattggat aataaggcgc tttctgaaag gacgtatacg tcagatggta ggataacatt tgcgaagttc aattcggatc tagttcctta ttcacgtaaa caagcacctg aagttcttaa gacgtattta caattaccct taacttctca agctacattc ttgcgttcgg ctcgtgtagg agattttgat cacgatcgtt ttcaacagag atataaaggt gttagagtag agaatggaat ttatacagtg gtctcaaaag agaatacgat agaactgatg atgggtgaat tttaccaagt tccagagaat tttaattcct ctcctaaact atccgagtca gaagcacttt caaagacttt aaaacatatc ggagctaaga agtatatttg ggaatctgtt gaacgagaag cagcattgaa acgtaagaaa aatgatccaa atgcaagtta ttttccgaag ggagaactat tagtttatga acattcatta ggaaaatcca atactaaaaa agagtttcga ttggcttata aatttggtat agcctcgata gatccaccaa tatcgagata cgtgtatgtg gattctaact ctggggaaat tctatccagt aaagatgcta gaagatacga agcgggtgga ggagatacaa ttccaacgac tccaccaact cctccggata caaattacgg aatttgtttt ccggatcaaa ctccttgtat caaacaagga tcagcgtcta ctcgttttag cggagtgaaa acgattacaa cttatactgc aggagaagaa aatcattatg aactgaagga ttattcgaga ggtaaaggaa tcattacgta ttcttgggaa tttatagaag atccatttat gggtatttat ttattgaatg ttcccattgt tgatattaat aacggttgga cgaaatttga atatcatgac gattataatc atgatgcatt attggatgca cattggggaa ttgaaatgac gtatgattat ttcaaatccg ttcataatcg gttgagttat gatggaaatg atagcaaagt agtgaataac gtgcactatt ttaatatttt cgtaggtaat aatgcatact gggatcctat gacagaggaa atctattata tatactgtcc tcataacagt agtacatgca aaagtcttaa tttacctatt attttagacc ctacatatga agattttact tctttggata ttgtatctca tgaatttgga catggtatca acggggatct tgcaggtttt acttatgatc cagagccagg tgctcttgat gaaggtttta gtgatatttg gaatgttggt gtaaataact acgtaaataa agttttagga atgcaaaaga acatttggtt agtaggagat gaaacggttc cgggaggagg aatgcgttct gtgtcaaatc caaaatctac tacagtaatg tcccctgggc caaatacgta ttatggaggt ctttgggatt ctgaaaataa cgaagcgcat acgaatagtc ttgttttgag tcattggttc tatactttat ccaagggaaa acaagggatc aatgatcatt ggtgtgagta taacgtttct ggaatcaaca ttgaaaaggc ggaaaaaatc gcttatgctg ctctctatta tcttttccct acatccggtt atacttctgc aaggaccgct gcgatttatt cggctaaggc tttgtatggt aagttttcat cggaagtaaa aagtacaatt gatgcttggg atgcaatagg tgttccagct gatacaactt ctcgcggtgg acaaggaatg tataagccgc actattatat cacttttgta aaactttcta atttagagag aagttccgga aatgattgtg ggtacaagga taattcatac ctacatccta ctgtcattaa aggatttacg tataacatgg tgttatcgag cgaaggtgag gtaagtattc cgtctaaggt tcacaaatgg agagtatgga tcgatttcaa tcgaaatggt agttttgaat cctcggaaat ggtagttcag gattctatca acgatacatt tggaggaaca cttcaaaaat cgattcagat tccaacgagt gcgcttactg gcgatacgag gatgagagtt tctatgaagg cagtccaaag cggagaaagt tatcaacttg cgaatgaatc ctttaccgaa ggagaagttg aagattattc gataactatc aataatttca gtatttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat