LpinA.19553.a

Thermolysin homolog

CENTER ID: LpinA.19553.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19553.a.B2.GE41960 mature protein 31 792
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.4023
UniProt: Q72M69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLLKLLKIN SNIKILLSMF MISILFSCKD KQGPGIESWL WVLNPVQLDS KALSERTYTS DGRITFAKFN SDLVPYSRKQ APEVLKTYLQ LPLSSEATFL RSARVGNYDQ DRFQQRYKGI RVENGIYTVV SKENTIELMM GEFHQVPENF NSSPKLSESE ALSKTLKHIG AKKYIWESVE REAALKRKKN DPNASYFPKG ELLVYEHSLG KSNTKKEFRL AYKFGIASID PPISRYVYVD SNSGEILSSK DARRYEGVQF PSPKPPIEID IDYSRCIIDR EYCIEQGTAM TRFSGLQSIT TWTAGKTNHF ELKDNSRGGG IYTYSWEFVK DPLEDIQLLN IPMIDTNNSW SQSEYHDDYN HDALLDAHWG IEMTYDYFKS IHNRLSYDGD NSRIFNNVHY FNAFVANNAY WDPVTEEIYY VYCPHKNSIC KGFNLPILLD PKYEDFTSLD IVSHEFGHGI NGDLAGFNLD MEPGALDEGF SDIWNVGVNN YVNKVLGMQK NIWLVGDETV PGGGMRSVSN PKSTTVMSPG PNTYHGDLWD FEDNEAHTNS LVLSHWFYTL SKGKLGFNDY ECTYNVSGIG IEKAERIAYV ALLFLSSTSG YTSARTYAII AAKLLYGLFS SEVKSTIDAW DAVAVPAETT SRGGQGMVRP RHYIASVKLS NVTNDSGNDC GYKDNSYLLP TVLRGVTYNM VLLSQGSASN PSKVHKWRVW IDFNQNGSFE SSEMVVQDTV NSSFGGTLQK SIKIPTNALT GYAKMRVSMK AAQSGEAYQG SSESFVEGEV EDYIVSILDF SL
NT Sequence
atgagacttt taaagttatt aaagatcaat tcgaatataa agatactctt aagtatgttt atgatttcga tattattttc ttgtaaggat aaacagggcc cgggaataga atcttggttg tgggttttaa atcctgttca attggatagt aaggcgcttt ctgaaaggac gtatacgtca gatggtagga taacatttgc gaagttcaat tcggatctag ttccttattc acgtaaacaa gcacctgaag ttcttaagac gtatttgcaa ttacccttaa gttctgaagc tacattcttg cgttcggctc gtgtaggtaa ttatgatcag gatcggttcc agcagagata taaaggtatt agagtagaga atggaattta tacagtggtc tcaaaagaga atacgataga attgatgatg ggtgaatttc accaagttcc agagaatttt aattcctctc ctaagctatc cgagtcagaa gcactttcaa agactttaaa acatatcgga gctaagaagt atatttggga atctgttgaa cgagaagcag cattgaaacg taagaaaaat gatccaaatg caagttattt tccgaaggga gaactattag tttatgaaca ttcattagga aaatccaata ctaaaaaaga gtttcgattg gcttataaat ttggtatagc ctcgatagat ccaccaatat cgagatacgt gtatgtggat tctaactctg gggaaattct atccagtaaa gatgctagaa gatacgaggg agttcaattt ccaagtccaa aacctcctat agaaatagat attgattata gtcgttgtat aatagacaga gaatattgta tcgaacaggg aacggcaatg acccgtttta gcggattaca atcgattaca acttggacgg caggaaaaac gaaccatttt gaactgaaag ataattcgag aggcggtgga atttatactt actcttggga atttgtaaaa gatcctttgg aagatattca acttttgaat attccaatga ttgatactaa taactcatgg tcgcaatctg aatatcatga cgattataat catgatgcgt tattggatgc acattgggga attgaaatga cttatgacta tttcaaatcc attcataatc ggttgagtta tgatggtgat aatagtagaa tatttaataa cgtgcactat tttaatgcgt ttgtagcgaa taacgcgtat tgggatcctg ttacggaaga aatatattac gtttactgtc ctcataaaaa tagtatatgc aagggtttta atctaccgat tctcttagac cctaaatatg aagattttac ttctttggat attgtatctc atgaatttgg gcatggtatc aacggggatc ttgcgggttt taatcttgat atggagccag gcgctctcga tgaaggtttt agcgatattt ggaatgttgg tgtaaataac tacgtaaata aagttttagg aatgcaaaag aatatttggt tagtaggaga tgaaacggtt ccgggaggag gaatgcgttc tgtgtcaaat ccaaaatcta ctacagtaat gtcccctggg ccaaatacgt atcatggaga tctttgggat tttgaagata acgaagcaca tacgaatagt cttgttttga gtcactggtt ctatacttta tccaaaggaa aattaggatt taacgattat gaatgcacgt ataacgtttc tggaattggc attgaaaaag ccgaaagaat tgcttatgtt gcccttcttt ttctttcttc tacgtccggt tatacttctg caaggactta tgcgattatt gcggctaaac tattgtatgg tttgttttct tcggaagtaa aaagtacaat tgatgcttgg gatgcagtag ctgttccggc tgagacgact tctcgcggag gacaaggtat ggtaaggcct cgacattata tcgcttctgt aaaactttct aatgtaacga acgattccgg aaacgattgt gggtacaagg ataattcata cttacttcca acggtcttga gaggagtgac gtataacatg gtgttattga gtcagggttc ggcaagtaat ccgtcaaaag ttcacaaatg gagagtatgg atcgatttta atcaaaacgg tagttttgaa tcgtcggaaa tggtagttca ggatactgtt aattcttcgt ttggaggaac attacaaaaa tcgattaaga tcccaacgaa tgcacttact ggatatgcga agatgagagt ttctatgaag gcggctcaaa gtggagaagc ttatcaaggt tcgagtgaaa gctttgtaga aggagaagtg gaggattata ttgtttcgat tctagatttc agtttatag
Details for LpinA.19553.a.B2.GE41960
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 39
Validated AA Sequence
MAHHHHHHMK QGPGIESWLW VLNPVQLDSK ALSERTYTSD GRITFAKFNS DLVPYSRKQA PEVLKTYLQL PLSSEATFLR SARVGNYDQD RFQQRYKGIR VENGIYTVVS KENTIELMMG EFHQVPENFN SSPKLSESEA LSKTLKHIGA KKYIWESVER EAALKRKKND PNASYFPKGE LLVYEHSLGK SNTKKEFRLA YKFGIASIDP PISRYVYVDS NSGEILSSKD ARRYEGVQFP SPKPPIEIDI DYSRCIIDRE YCIEQGTAMT RFSGLQSITT WXAGKTNHFE LKDNSRGGGI YTYS
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa cagggcccgg gaatagaatc ttggttgtgg gttttaaatc ctgttcaatt ggatagtaag gcgctttctg aaaggacgta tacgtcagat ggtaggataa catttgcgaa gttcaattcg gatctagttc cttattcacg taaacaagca cctgaagttc ttaagacgta tttgcaatta cccttaagtt ctgaagctac attcttgcgt tcggctcgtg taggtaatta tgatcaggat cggttccagc agagatataa aggtattaga gtagagaatg gaatttatac agtggtctca aaagagaata cgatagaatt gatgatgggt gaatttcacc aagttccaga gaattttaat tcctctccta agctatccga gtcagaagca ctttcaaaga ctttaaaaca tatcggagct aagaagtata tttgggaatc tgttgaacga gaagcagcat tgaaacgtaa gaaaaatgat ccaaatgcaa gttattttcc gaagggagaa ctattagttt atgaacattc attaggaaaa tccaatacta aaaaagagtt tcgattggct tataaatttg gtatagcctc gatagatcca ccaatatcga gatacgtgta tgtggattct aactctgggg aaattctatc cagtaaagat gctagaagat acgagggagt tcaatttcca agtccaaaac ctcctataga aatagatatt gattatagtc gttgtataat agacagagaa tattgtatcg aacagggaac ggcaatgacc cgttttagcg gattacaatc gattacaact tggnnggcag gaaaaacgaa ccattttgaa ctgaaagata attcgagagg cggnggaatt tatacttact ct
Expected Protein Sequence
MAHHHHHHKQ GPGIESWLWV LNPVQLDSKA LSERTYTSDG RITFAKFNSD LVPYSRKQAP EVLKTYLQLP LSSEATFLRS ARVGNYDQDR FQQRYKGIRV ENGIYTVVSK ENTIELMMGE FHQVPENFNS SPKLSESEAL SKTLKHIGAK KYIWESVERE AALKRKKNDP NASYFPKGEL LVYEHSLGKS NTKKEFRLAY KFGIASIDPP ISRYVYVDSN SGEILSSKDA RRYEGVQFPS PKPPIEIDID YSRCIIDREY CIEQGTAMTR FSGLQSITTW TAGKTNHFEL KDNSRGGGIY TYSWEFVKDP LEDIQLLNIP MIDTNNSWSQ SEYHDDYNHD ALLDAHWGIE MTYDYFKSIH NRLSYDGDNS RIFNNVHYFN AFVANNAYWD PVTEEIYYVY CPHKNSICKG FNLPILLDPK YEDFTSLDIV SHEFGHGING DLAGFNLDME PGALDEGFSD IWNVGVNNYV NKVLGMQKNI WLVGDETVPG GGMRSVSNPK STTVMSPGPN TYHGDLWDFE DNEAHTNSLV LSHWFYTLSK GKLGFNDYEC TYNVSGIGIE KAERIAYVAL LFLSSTSGYT SARTYAIIAA KLLYGLFSSE VKSTIDAWDA VAVPAETTSR GGQGMVRPRH YIASVKLSNV TNDSGNDCGY KDNSYLLPTV LRGVTYNMVL LSQGSASNPS KVHKWRVWID FNQNGSFESS EMVVQDTVNS SFGGTLQKSI KIPTNALTGY AKMRVSMKAA QSGEAYQGSS ESFVEGEVED YIVSILDFSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaacagg gcccgggaat agaatcttgg ttgtgggttt taaatcctgt tcaattggat agtaaggcgc tttctgaaag gacgtatacg tcagatggta ggataacatt tgcgaagttc aattcggatc tagttcctta ttcacgtaaa caagcacctg aagttcttaa gacgtatttg caattaccct taagttctga agctacattc ttgcgttcgg ctcgtgtagg taattatgat caggatcggt tccagcagag atataaaggt attagagtag agaatggaat ttatacagtg gtctcaaaag agaatacgat agaattgatg atgggtgaat ttcaccaagt tccagagaat tttaattcct ctcctaagct atccgagtca gaagcacttt caaagacttt aaaacatatc ggagctaaga agtatatttg ggaatctgtt gaacgagaag cagcattgaa acgtaagaaa aatgatccaa atgcaagtta ttttccgaag ggagaactat tagtttatga acattcatta ggaaaatcca atactaaaaa agagtttcga ttggcttata aatttggtat agcctcgata gatccaccaa tatcgagata cgtgtatgtg gattctaact ctggggaaat tctatccagt aaagatgcta gaagatacga gggagttcaa tttccaagtc caaaacctcc tatagaaata gatattgatt atagtcgttg tataatagac agagaatatt gtatcgaaca gggaacggca atgacccgtt ttagcggatt acaatcgatt acaacttgga cggcaggaaa aacgaaccat tttgaactga aagataattc gagaggcggt ggaatttata cttactcttg ggaatttgta aaagatcctt tggaagatat tcaacttttg aatattccaa tgattgatac taataactca tggtcgcaat ctgaatatca tgacgattat aatcatgatg cgttattgga tgcacattgg ggaattgaaa tgacttatga ctatttcaaa tccattcata atcggttgag ttatgatggt gataatagta gaatatttaa taacgtgcac tattttaatg cgtttgtagc gaataacgcg tattgggatc ctgttacgga agaaatatat tacgtttact gtcctcataa aaatagtata tgcaagggtt ttaatctacc gattctctta gaccctaaat atgaagattt tacttctttg gatattgtat ctcatgaatt tgggcatggt atcaacgggg atcttgcggg ttttaatctt gatatggagc caggcgctct cgatgaaggt tttagcgata tttggaatgt tggtgtaaat aactacgtaa ataaagtttt aggaatgcaa aagaatattt ggttagtagg agatgaaacg gttccgggag gaggaatgcg ttctgtgtca aatccaaaat ctactacagt aatgtcccct gggccaaata cgtatcatgg agatctttgg gattttgaag ataacgaagc acatacgaat agtcttgttt tgagtcactg gttctatact ttatccaaag gaaaattagg atttaacgat tatgaatgca cgtataacgt ttctggaatt ggcattgaaa aagccgaaag aattgcttat gttgcccttc tttttctttc ttctacgtcc ggttatactt ctgcaaggac ttatgcgatt attgcggcta aactattgta tggtttgttt tcttcggaag taaaaagtac aattgatgct tgggatgcag tagctgttcc ggctgagacg acttctcgcg gaggacaagg tatggtaagg cctcgacatt atatcgcttc tgtaaaactt tctaatgtaa cgaacgattc cggaaacgat tgtgggtaca aggataattc atacttactt ccaacggtct tgagaggagt gacgtataac atggtgttat tgagtcaggg ttcggcaagt aatccgtcaa aagttcacaa atggagagta tggatcgatt ttaatcaaaa cggtagtttt gaatcgtcgg aaatggtagt tcaggatact gttaattctt cgtttggagg aacattacaa aaatcgatta agatcccaac gaatgcactt actggatatg cgaagatgag agtttctatg aaggcggctc aaagtggaga agcttatcaa ggttcgagtg aaagctttgt agaaggagaa gtggaggatt atattgtttc gattctagat ttcagtttat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat