SSGCID: Target Page

LpinA.19552.c

Adenylate/guanylate cyclase

CENTER ID:	LpinA.19552.c
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19552.c.B2.GE42902	soluble domain		442	758

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.29
UniProt:	Q72WB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIRLIRTFAY ILVFWILCSC VSDSDRFQNL DLITQAEWTA SLGNHLKDRL EKSNVQKSFL KNKKEKLNIE TNSREKESKD FKNWKLITRF PVNFNFLFSI PKQSGFHDVT VKVEFSIHSD SIFLTFLKQP TAIYFPDLGE NWELYLNGIP IRKEKFLKQT DTGDLIPSVR RSLKSVILPI PSGVLKEGKN TILIYMIGES DFTHYIPNEH FGFYHSKGYK ILSLEQIYES TFEYWDLLLY GIYTIFGIYH IYFYFIRRKD LYYLYFGLFS FFSSVYFFSS SNLIFQKFVN PNPELDSSLF FRMQYSSLTL IFPSFYYFLK DYFSPKEKGS VIASFFVIFL IGLFFAFLTV PFSWVHIAFK ICQILAICFS VYILFFSIQT VRKKKQDARK ILVGICVCIV FSTWELYGSV FENAKYHSTF FKLVYLFFII SIASTLVLRY VQLYKDAQLL NKYLIDQKEA FYRFVPVDFI RIIDRESPIS ISVGDSKEKS MTVLTSCIRN FASLSKTVPL NQTITFLNSY LSEMEELVYK TTGFVDKYIG NEILALFGDY DERAAKENFN SADNAVESAI KMVNAIRSGK LVKNFLIPSF WNLEIGIGIN TGSVILGTVG SERRIDTTVI GDAVDLSSGL RSLTALYKSK ILISHHTYLR LHRMSEIGIR MIDSVILKER DQAVDIYEVI ESDPESIKEF KLKTLELLSQ GILEYKSKRF DEAAKIFRQL YREEPKDFIS MIYLKRCKLY SSKPPDENWD GVFRFQTK

NT Sequence

atgattcggc ttattagaac ttttgcatac atattagtct tttggatttt atgttcttgt gtatctgatt ccgatcgttt tcaaaattta gatctaataa cacaagcgga atggactgct agtttaggaa atcatttaaa agatagatta gaaaagtcaa atgttcagaa atcttttcta aaaaacaaaa aagaaaaact gaatattgaa acgaattcta gggaaaaaga atcaaaagat tttaaaaatt ggaaattaat tacacgcttt cccgttaact tcaattttct tttttccata ccgaaacaat ctggatttca cgatgtaacc gtaaaagtag aattttctat tcattcggat tcaatatttc taacattttt aaaacaaccg actgcaattt atttcccaga tctgggagaa aactgggaac tttatctcaa cggaatacca attcgaaaag aaaaatttct aaaacaaacc gataccggag atttaattcc ttcggttcga agatctttaa aatccgtaat tttaccaatt ccatctgggg ttttaaaaga agggaaaaat acaattttaa tatatatgat cggagaatcc gattttacac attatattcc aaatgaacat ttcggatttt atcattcaaa aggatataaa attttatctt tagaacaaat ttatgaatct acctttgaat attgggattt gttgttatat ggaatttata ctatattcgg aatttatcat atttattttt actttattcg aagaaaagat ttatattatc tctatttcgg attgttctcg tttttttctt cggtttattt tttttcttct tccaatttga tctttcaaaa attcgtaaat ccaaatccgg aactagacag ttctcttttt tttaggatgc agtattcttc tttgactctg atttttcctt ctttctatta ttttttaaag gattatttct ctccaaaaga aaaagggagt gtgattgctt ctttttttgt aatttttttg atcgggttgt tttttgcgtt tttaaccgtt ccattttctt gggtgcatat cgcctttaaa atttgtcaaa ttttggcgat ttgtttttca gtttatattt tatttttttc gattcaaacc gtaagaaaaa agaaacaaga tgcacgtaaa atattggtgg ggatttgcgt atgtattgtt ttttctactt gggaattata cggctcggtt tttgaaaatg caaagtatca ttctactttt tttaagcttg tttatttatt ttttatcata tcaatcgcca gcacattagt tctaagatac gttcaacttt acaaagacgc tcagctttta aataaatatc ttatcgatca gaaagaagct ttttatcgat ttgttcctgt ggattttatt agaattatag atagagaatc tcccatatcg atttcggtcg gcgatagtaa agaaaaatca atgactgttt tgacttcttg tattcgaaat tttgcaagtc tttctaaaac agttccatta aatcaaacaa ttacgttttt aaattcttat ttaagcgaaa tggaagaatt ggtttataaa accactggtt ttgtagataa atatatagga aatgagatac ttgcactttt tggagactac gacgaaagag cagcaaaaga aaatttcaat tctgcagaca acgcggtgga atcagcaatc aaaatggtaa atgcgatccg atctggaaaa ttagtaaaaa attttttgat accatctttt tggaacttag agatcggcat tgggatcaat accggttctg tgattttagg aaccgtagga agtgaaagaa gaatcgatac aactgtaatt ggggatgcag ttgatctttc atctggactt cgatctctta cggcacttta caaaagtaaa atattaattt cccatcatac gtatcttaga cttcatagaa tgagtgaaat aggaatacgt atgatcgatt cggttattct aaaagaaaga gatcaagccg tggatattta tgaggtaatc gaatccgatc ccgaatcaat caaggaattt aaactgaaaa ctttagagtt attgtctcaa ggaattttag aatataaatc caagaggttt gacgaagccg ctaaaatttt tagacaactc tatcgagaag aaccaaaaga ttttatttct atgatttatt taaaacgttg taaactatat tcttccaaac ccccagatga gaattgggat ggagttttta ggtttcagac aaagtag

Details for LpinA.19552.c.B2.GE42902

HARVESTED ON:	2/7/2019
SEQUENCED ON:	2/8/2019
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHQL YKDAQLLNKY LIDQKEAFYR FVPVDFIRII DRESPISISV GDSKEKSMTV LTSCIRNFAS LSKTVPLNQT ITFLNSYLSE MEELVYKTTG FVDKYIGNEI LALFGDYDER AAKENFNSAD NAVESAIKMV NAIRSGKLVK NFLIPSFWNL EIGIGINTGS VILGTVGSER RIDTTVIGDA VDLSSGLRSL TALYKSKILI SHHTYLRLHR MSEIGIRMID SVILKERDQA VDIYEVIESD PESIKEFKLK TLELLSQGIL EYKSKRFDEA AKIFRQLYRE EPKDFISMIY LKRCKLYSSK PPDENWDGVF RFQTK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaacttt acaaagacgc tcagctttta aataaatatc ttatcgatca gaaagaagct ttttatcgat ttgttcctgt ggattttatt agaattatag atagagaatc tcccatatcg atttcggtcg gcgatagtaa agaaaaatca atgactgttt tgacttcttg tattcgaaat tttgcaagtc tttctaaaac agttccatta aatcaaacaa ttacgttttt aaattcttat ttaagcgaaa tggaagaatt ggtttataaa accactggtt ttgtagataa atatatagga aatgagatac ttgcactttt tggagactac gacgaaagag cagcaaaaga aaatttcaat tctgcagaca acgcggtgga atcagcaatc aaaatggtaa atgcgatccg atctggaaaa ttagtaaaaa attttttgat accatctttt tggaacttag agatcggcat tgggatcaat accggttctg tgattttagg aaccgtagga agtgaaagaa gaatcgatac aactgtaatt ggggatgcag ttgatctttc atctggactt cgatctctta cggcacttta caaaagtaaa atattaattt cccatcatac gtatcttaga cttcatagaa tgagtgaaat aggaatacgt atgatcgatt cggttattct aaaagaaaga gatcaagccg tggatattta tgaggtaatc gaatccgatc ccgaatcaat caaggaattt aaactgaaaa ctttagagtt attgtctcaa ggaattttag aatataaatc caagaggttt gacgaagccg ctaaaatttt tagacaactc tatcgagaag aaccaaaaga ttttatttct atgatttatt taaaacgttg taaactatat tcttccaaac ccccagatga gaattgggat ggagttttta ggtttcagac aaagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat