LpinA.19552.a

Adenylate/guanylate cyclase

CENTER ID: LpinA.19552.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19552.a.B2.GE42900 soluble domain #1 1 168
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.3205
UniProt: Q72P07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEDKTFLFC SRLKEGGMLD RWKDKNDKSV RWEELPNLHS DLQDLDLIIR SLISEKLSLG IREIIFCLSA EQALSLKKSR DFLKLIKDLK RAGFTISSAD KDKILSTNVE SSSKFFRYLE NRSRNVNCLV WTEDLALSQI ESKYYDFLNH FHGYKFESFR PSRFTIRVKL LSIVSAIITL SMGLMITMAT YFFRKYSEIL IQEYNLSLAR MTGIQLSGQL KETTRKIQDF RSEDSEKFFR TNSNAVAYVR FKNKTVNSAL EIDSLFWNSK FLKSNSVVPD PTNLKNALEK SFSRLSGTLE ILNVSNEFGF PAVAVLLPVG VEISGLVFSA SEFLGSFLSV RQTDFFQMIV VDSSGNLIAH SNDKEAVSGK DYKKHPLVEN MLRSPSDNGS QRFDFEGREV LGSYQQLEVG GLGIISTLDA DIAFEAVYKI RRQNFWIMIS VLSVAFFVVF VFSRTLTIPI IQLLSATKKV EQGNYAVDIR ATTHDEVGVL TNSFLRMARG LEEREKIKNT FGKFVNKEIA ERALSSDLKL GGENREVTVF FSDLRNFTGM SEKMKPEEVV EFLNQYFTEM VECIYLTQGI VDKFIGDAVM AHWGALVYDG NEAKNSINAA LLMRRALIEF NLKGKEIGRP FTRFGCGINS GPVIVGQIGS EKKLEFTVIG DTVNLASRIE YLNKEFGTDI LISESTYQQA KDHFNFVELP PVWIRGKEKP QVVYAVLGWK DDQDCPKSLE ELRTLCGIPD PENPSRSLA
NT Sequence
atgacagaag ataaaacgtt cttattctgc tccagactta aggaaggtgg gatgttggat cgatggaagg ataaaaatga caaatcggtt cgttgggaag agctaccaaa tcttcattct gatttgcagg atttggatct gatcatacga agtttgattt cggaaaaact ttctcttgga attcgagaaa tcattttttg tctttctgca gaacaagctc tatctctaaa aaaatccagg gattttttaa aacttataaa agatcttaaa agagctggtt ttactatttc atctgcggac aaggataaaa tactttccac aaacgtagaa tcttcttcta aattctttcg ttatttagaa aaccgttcta gaaatgtaaa ttgtttggtt tggacagagg atttagcact ttctcagatc gaatcaaaat attatgattt tcttaatcat ttccacggtt acaaatttga atcatttcgt ccgtccaggt ttacaattcg tgtaaaacta ctttctatcg tttctgcgat tatcactctt agtatgggcc tgatgattac tatggctacg tactttttta ggaaatacag cgaaattcta attcaagaat ataatctatc tcttgctaga atgacaggca ttcaattgag cggacaattg aaagaaacca ctcgtaaaat tcaggatttt cgttctgaag attctgaaaa attttttaga accaattcaa acgcggttgc atacgtacgt tttaaaaaca agacggtaaa ttccgctttg gaaatagatt cccttttttg gaattcaaaa ttcttaaagt ctaattcggt tgtacctgat cctacaaatc taaaaaatgc gttggaaaaa tctttttccc gtttgtcagg tacattagaa atattgaatg tttctaatga gtttggtttc cctgcggttg cagttttgct tccggttgga gtcgaaattt caggtttggt tttttccgct tccgaatttt taggttcttt tcttagtgtt agacaaaccg atttttttca aatgatcgtg gtggattctt ctggaaactt gatcgctcat tctaacgata aagaggcggt ttccggaaag gattataaaa aacatcctct tgtggaaaat atgttacgta gtccttcgga caatggttct caacgattcg atttcgaagg tagagaagta ttaggttctt atcaacaatt ggaagtgggt ggtcttggaa ttatttctac gttagacgct gatattgcct ttgaagcggt gtataagatc agaagacaaa atttttggat tatgatttct gttttgtcag ttgcgttttt tgtagtgttc gttttttcaa gaacccttac gattccgatc attcaacttc tttctgcaac taaaaaagtg gaacaaggaa actacgcggt ggatattcgt gctactacac acgacgaagt aggagttcta acaaattcgt ttttaagaat ggcgagggga ttggaagaaa gagaaaaaat taaaaataca ttcggaaagt ttgtgaataa agagatcgcc gaacgcgcgt tatcttccga tttaaagttg ggcggagaaa atagggaggt cacggtattt ttttctgacc ttagaaattt tacgggaatg tccgaaaaga tgaaaccgga agaggtggtt gaattcttaa atcaatattt caccgaaatg gtggaatgta tttatctgac tcagggaatt gtggataaat ttataggtga cgccgtgatg gcccactggg gagctttggt ttacgatgga aatgaagcta aaaattcgat taacgccgct ttgttgatga gaagagcgtt gatagagttt aatttaaaag gaaaagaaat cggtcgtcct tttactcgtt tcggttgtgg gatcaattct ggaccggtga tcgttgggca gatcggctct gaaaagaagt tagaatttac ggtcatcgga gatacggtca atcttgcttc tagaattgaa tatttaaata aggaattcgg tacggacatc ttaatttcag aaagtacgta tcaacaagca aaggatcatt ttaattttgt ggagctccca cccgtttgga ttcgaggtaa ggaaaaacca caagtcgtct atgcagtgtt aggttggaaa gacgatcagg attgtcctaa gtccttggaa gaattacgaa cgttatgcgg aattccggat cctgaaaacc cttctcggtc tttggcatga
Details for LpinA.19552.a.B2.GE42900
HARVESTED ON: 2/7/2019
SEQUENCED ON: 2/8/2019
EXPECTED MW: 21kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT EDKTFLFCSR LKEGGMLDRW KDKNDKSVRW EELPNLHSDL QDLDLIIRSL ISEKLSLGIR EIIFCLSAEQ ALSLKKSRDF LKLIKDLKRA GFTISSADKD KILSTNVESS SKFFRYLENR SRNVNCLVWT EDLALSQIES KYYDFLNHFH GYKFESFRPS RFTIRV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag aagataaaac gttcttattc tgctccagac ttaaggaagg tgggatgttg gatcgatgga aggataaaaa tgacaaatcg gttcgttggg aagagctacc aaatcttcat tctgatttgc aggatttgga tctgatcata cgaagtttga tttcggaaaa actttctctt ggaattcgag aaatcatttt ttgtctttct gcagaacaag ctctatctct aaaaaaatcc agggattttt taaaacttat aaaagatctt aaaagagctg gttttactat ttcatctgcg gacaaggata aaatactttc cacaaacgta gaatcttctt ctaaattctt tcgttattta gaaaaccgtt ctagaaatgt aaattgtttg gtttggacag aggatttagc actttctcag atcgaatcaa aatattatga ttttcttaat catttccacg gttacaaatt tgaatcattt cgtccgtcca ggtttacaat tcgtgtatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at