SSGCID: Target Page

LpinA.19130.a

Probable endolytic peptidoglycan transglycosylase RlpA (EC 4.2.2.-)

CENTER ID:	LpinA.19130.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19130.a.B2.GE42899	mature protein		41	321

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.63
UniProt:	Q72W83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVFSRSTDLF KGINRTEETM KQIAILTALI IFTSCASVES KRSVSASGDP SEIFFEKEII PMDSNSNFVS QKPARRSFEE ELSVEKYAKA QPPEKTNSSG DFDEVGMSSW YGAKFHGKPT ASGEKFDKTK LTAAHPTLPL GSIIRVQNLE NQKEVIVRVN DRGPFVKDRI IDLSEKAADT LDFKDVGIAK VGIKVVKRGG AANEESEDLE NSDDEEALLE DGKPEKLNPQ KSDYQNKPIA GGKYIKGAPK GYTVQVGVFR EQSRAESYKS NLGQEYGEKT FLFTRDGLFV IQLGDFASRT EAESLKSKLK NDGIDCFIPK K

NT Sequence

atggtttttt ccagatcaac cgatttgttt aaaggaatca ataggaccga ggaaactatg aaacaaatag caatcttaac cgccctgatc atttttacgt cttgtgcgtc ggtagagtct aaacgaagcg tcagcgcttc tggagatccg tcggagattt tttttgaaaa agaaattatt ccgatggatt ctaattcaaa tttcgtttct caaaaacctg cacgtagatc tttcgaagaa gaattgagtg ttgaaaagta tgcaaaagct caaccacctg aaaaaaccaa ttcttctgga gattttgacg aagttggaat gtcttcctgg tatggcgcaa agtttcacgg taaaccaacg gcaagcggag aaaaatttga taaaacaaaa ctaactgctg cacatccaac acttccttta ggttccatca ttagagttca aaatttagaa aaccaaaaag aagttatagt tcgtgtcaac gatagaggac cttttgtaaa ggatagaatc attgatcttt ctgaaaaagc tgcagatact ttagatttta aagatgtagg tattgctaaa gtaggtatta aagtagtaaa acgtggagga gctgcaaacg aagaatccga agatctcgaa aattctgacg atgaagaagc tcttttagaa gatggaaaac ctgaaaaact aaatcctcaa aaatcggatt atcaaaacaa accgattgcc ggtggaaagt atatcaaagg tgctcctaaa ggatatacgg ttcaagtagg tgtttttcgg gaacaatcta gagctgaatc ttataaatct aatcttggac aagaatacgg tgaaaaaact ttcctattta caagagatgg tttatttgta attcagttag gggatttcgc aagcagaact gaggcggaat cgttgaaatc gaaattaaaa aacgatggaa ttgactgttt cattccgaaa aaataa

Details for LpinA.19130.a.B2.GE42899

HARVESTED ON:	2/7/2019
SEQUENCED ON:	2/8/2019
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHKR SVSASGDPSE IFFEKEIIPM DSNSNFVSQK PARRSFEEEL SVEKYAKAQP PEKTNSSGDF DEVGMSSWYG AKFHGKPTAS GEKFDKTKLT AAHPTLPLGS IIRVQNLENQ KEVIVRVNDR GPFVKDRIID LSEKAADTLD FKDVGIAKVG IKVVKRGGAA NEESEDLENS DDEEALLEDG KPEKLNPQKS DYQNKPIAGG KYIKGAPKGY TVQVGVFREQ SRAESYKSNL GQEYGEKTFL FTRDGLFVIQ LGDFASRTEA ESLKSKLKND GIDCFIPKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaacgaa gcgtcagcgc ttctggagat ccgtcggaga ttttttttga aaaagaaatt attccgatgg attctaattc aaatttcgtt tctcaaaaac ctgcacgtag atctttcgaa gaagaattga gtgttgaaaa gtatgcaaaa gctcaaccac ctgaaaaaac caattcttct ggagattttg acgaagttgg aatgtcttcc tggtatggcg caaagtttca cggtaaacca acggcaagcg gagaaaaatt tgataaaaca aaactaactg ctgcacatcc aacacttcct ttaggttcca tcattagagt tcaaaattta gaaaaccaaa aagaagttat agttcgtgtc aacgatagag gaccttttgt aaaggataga atcattgatc tttctgaaaa agctgcagat actttagatt ttaaagatgt aggtattgct aaagtaggta ttaaagtagt aaaacgtgga ggagctgcaa acgaagaatc cgaagatctc gaaaattctg acgatgaaga agctctttta gaagatggaa aacctgaaaa actaaatcct caaaaatcgg attatcaaaa caaaccgatt gccggtggaa agtatatcaa aggtgctcct aaaggatata cggttcaagt aggtgttttt cgggaacaat ctagagctga atcttataaa tctaatcttg gacaagaata cggtgaaaaa actttcctat ttacaagaga tggtttattt gtaattcagt taggggattt cgcaagcaga actgaggcgg aatcgttgaa atcgaaatta aaaaacgatg gaattgactg tttcattccg aaaaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t