SSGCID: Target Page

LpinA.18992.a

BatA (LIC_20040)

CENTER ID:	LpinA.18992.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.18992.a.B1.GE43982	VWFA domain		91	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	Q75FZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MERNLGSKMN FQYPYALLLL IPVWIWAFIY YKNKIYLKKM EIRFPGRRES TFSKFENLGK FLPILRPIAI SLMILSLSGP GKKITFLPDE KEGVDILIAL DVSGSMSRSR DFLPETRLGV SKKLLKRFIE KRNNDRLGLV VFAGAAYLQA PLTGDRESLS EILETIEEET VTEQGTAIGD AIILSTYRLR NSKARSKVIV LITDGVSNTG KIDPVTATDL AEQIGAKIYS VGIGKEDGSY EINFEILQEL SANTGGRFFR AEDPEEMKAV LSSIDSLEKD PLQSPPKEIR ETEYEIWLYR ALAVLLLDLI LRAFFLRYYV

NT Sequence

ttggaaagga atctgggatc aaaaatgaat tttcaatatc cttacgcgct tttacttttg attccagttt ggatctgggc ttttatatat tataaaaaca aaatatactt aaaaaaaatg gaaattcgtt ttccaggaag aagagaaagt actttttcca aatttgaaaa cttagggaaa tttttaccga tacttcgccc gattgcgatt agtttgatga tactttctct ttccggacca ggtaaaaaaa ttacctttct acccgacgaa aaagaaggag tagatatatt aatcgccttg gatgtctcag gttccatgtc tagaagtaga gactttcttc cggaaactag attaggagtt tctaaaaaac tattaaaaag atttatcgaa aaaagaaata atgatcgtct tggtttagtg gtttttgcag gtgcggctta tttacaagct cctcttacag gtgatcgaga atctttgagc gaaattttag aaaccataga agaagaaaca gtgacggaac aagggaccgc aatcggagac gcgatcattc tttctacgta tcgattgagg aattctaagg ctcgttctaa agtgattgta ctcatcaccg atggagtttc caatacgggt aaaatcgatc cagtaacagc gactgatctt gcagaacaaa ttggagcaaa aatttattcc gtcggaattg gaaaagaaga cggttcttat gaaatcaatt tcgaaatttt gcaagaactt tctgccaata ctggaggcag attttttagg gcagaagatc cggaggaaat gaaagcggtt ctttcttcga tcgattctct tgaaaaagat cctctgcaaa gtcctcccaa agaaataaga gaaactgaat atgaaatctg gctctataga gccttagcgg ttttactttt agatttaata ctacgtgcgt tttttctgag atattatgta tga

Details for LpinA.18992.a.B1.GE43982

HARVESTED ON:	12/3/2020
SEQUENCED ON:	12/16/2020
EXPECTED MW:	25kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK EGVDILIALD VSGSMSRSRD FLPETRLGVS KKLLKRFIEK RNNDRLGLVV FAGAAYLQAP LTGDRESLSE ILETIEEETV TEQGTAIGDA IILSTYRLRN SKARSKVIVL ITDGVSNTGK IDPVTATDLA EQIGAKIYSV GIGKEDGSYE INFEILQELS ANTGGRFFRA EDPEEMKAVL SSIDSLEKDP LQSPPKEIRE TEYEIWLYRA LA

Validated NT Sequence

atggctcacc accaccacca ccatatgaaa gaaggagtag atatattaat cgccttggat gtctcaggtt ccatgtctag aagtagagac tttcttccgg aaactagatt aggagtttct aaaaaactat taaaaagatt tatcgaaaaa agaaataatg atcgtcttgg tttagtggtt tttgcaggtg cggcttattt acaagctcct cttacaggtg atcgagaatc tttgagcgaa attttagaaa ccatagaaga agaaacagtg acggaacaag ggaccgcaat cggagacgcg atcattcttt ctacgtatcg attgaggaat tctaaggctc gttctaaagt gattgtactc atcaccgatg gagtttccaa tacgggtaaa atcgatccag taacagcgac tgatcttgca gaacaaattg gagcaaaaat ttattccgtc ggaattggaa aagaagacgg ttcttatgaa atcaatttcg aaattttgca agaactttct gccaatactg gaggcagatt ttttagggca gaagatccgg aggaaatgaa agcggttctt tcttcgatcg attctcttga aaaagatcct ctgcaaagtc ctcccaaaga aataagagaa actgaatatg aaatctggct ctatagagcc ttagcgtaag tgagtaagat

Expected Protein Sequence

MAHHHHHHKE GVDILIALDV SGSMSRSRDF LPETRLGVSK KLLKRFIEKR NNDRLGLVVF AGAAYLQAPL TGDRESLSEI LETIEEETVT EQGTAIGDAI ILSTYRLRNS KARSKVIVLI TDGVSNTGKI DPVTATDLAE QIGAKIYSVG IGKEDGSYEI NFEILQELSA NTGGRFFRAE DPEEMKAVLS SIDSLEKDPL QSPPKEIRET EYEIWLYRAL A

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaagaag gagtagatat attaatcgcc ttggatgtct caggttccat gtctagaagt agagactttc ttccggaaac tagattagga gtttctaaaa aactattaaa aagatttatc gaaaaaagaa ataatgatcg tcttggttta gtggtttttg caggtgcggc ttatttacaa gctcctctta caggtgatcg agaatctttg agcgaaattt tagaaaccat agaagaagaa acagtgacgg aacaagggac cgcaatcgga gacgcgatca ttctttctac gtatcgattg aggaattcta aggctcgttc taaagtgatt gtactcatca ccgatggagt ttccaatacg ggtaaaatcg atccagtaac agcgactgat cttgcagaac aaattggagc aaaaatttat tccgtcggaa ttggaaaaga agacggttct tatgaaatca atttcgaaat tttgcaagaa ctttctgcca atactggagg cagatttttt agggcagaag atccggagga aatgaaagcg gttctttctt cgatcgattc tcttgaaaaa gatcctctgc aaagtcctcc caaagaaata agagaaactg aatatgaaat ctggctctat agagccttag cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat