LpinA.18975.a
Methyl-accepting chemotaxis protein
| CENTER ID: | LpinA.18975.a |
| ORGANISM: | Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130) |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LpinA.18975.a.B2.GE42898 | soluble domain #1 | 43 | 304 | ||
| LpinA.18975.a.B3.GE41957 | soluble domain #2 | 336 | 692 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6PZJ
DEPOSITED: 7/31/2019
DETERMINATION: XRay
CLONE: LpinA.18975.a.B2.GE42898
PROTEIN: LpinA.18975.a.B2.PW38653
Publications by SSGCID
Structural analysis of CACHE domain of the McpA chemoreceptor from Leptospira interrogans
Abendroth J, Lin T, Myler P, Staker B, Vieira M, Nascimento A, Santos JC
Biochem. Biophys. Res. Commun. - 2020
volume 533, issue 4, pages 1323-1329
PMID: 33097187; PMCID: PMC7744396
PDB:
6PZJ
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|267671.5.peg.3519 |
| UniProt: | Q72NB4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIRFRISLY LSIVLFIGFG ILASISCYTA YKKLEQEVVN SSEITAERWT YEVKDYLDTG MGIIRGFRFP LLFSAPPRNQ IIAALREILK VNDHYFGARL AYEPNSLDGN DLEFQNTLGH DSTGRFIPYL HRGQTKEEIV LEAAKYYDSL GPEGDWYQVP KKTKSHYATD PYYYEIKGKV KILMMSLMVP LYVNDQFYGV AGLDYQLEEL QQRIGVKKPF QDLGYLTLIS PKGIYAVNGF DSNRVGEKIS DAKELEYYLS KSQEGEKFTT DSDGYTHYYF PFHIGKDKRY WVMQVSIPNS IYKEAILSIL FKAFTYTLAV LIAVILCLNV IFQKLITAGL LKAVSFSEEI ADGNLIAQSS HDKKDEIGTL LSSMNKMREN LLKVLREIGG SANTLRDTSE KMADSSRNFS DVAQTQASAA EESSAAVEEL AASAQNVGKS MEKAVLSMKE IDGNVIRLKE QIVNINREMQ DLVELAALSK EQGVTGENAM IASTSAMAAI GDSASRITEI LSIITEISER TNLLALNAAI EAARAGEAGK GFAVVAEEIG KLASQTSTSV QEIGSLVNST NTAVLNGNTK VAEAFNILKK LREQVEEFDR YAKNVLTSVK TQEENTKEIA QSANELMTFS LQIEEAVLEQ RRATDEITKT IMSISDGTQE IASGAEDLTS FSGNMHGQAQ NLGKLIGKFK IN
NT Sequence
atgagcattc gttttcgcat ttctctctat ctttccatcg tgttattcat aggtttcggt attcttgcaa gtatcagttg ttacaccgca tataaaaagt tagaacaaga agttgtcaat agttcagaaa ttacagccga acgttggacc tatgaagtga aagattattt agacactggt atgggtatca tacgaggttt tcggtttcct cttctcttta gtgcccctcc ccgcaatcaa atcattgctg ctttaagaga aatcttaaaa gtcaatgatc attactttgg tgccagactc gcctacgaac caaattcttt ggacggaaac gacttagaat ttcaaaatac tctaggacat gattccacag gcagatttat tccgtatctt catcgtggtc aaacaaagga agaaatcgtt ctcgaagccg ctaaatacta cgattcttta ggtccggaag gagattggta ccaagttcct aaaaaaacaa aatctcacta cgcaacggat ccgtactatt atgaaatcaa agggaaagta aaaattctaa tgatgtctct tatggttccg ctttatgtaa acgatcagtt ttacggtgtt gccggtttag actatcaatt ggaagaactt caacaacgaa tcggcgttaa aaaaccgttt caagatcttg gttatctaac tctgatttct cctaaaggaa tttatgcagt aaatggtttt gattcaaatc gggtcggtga aaaaatttca gatgcaaaag aactagaata ttaccttagt aaaagtcagg aaggcgaaaa attcactact gattccgacg gatacactca ttattacttt ccgtttcata ttggtaaaga caaacgctat tgggtaatgc aagtaagtat tccaaattct atctataaag aagcaattct ttctattctt ttcaaagctt ttacgtacac cttagcggta ttaatcgccg tcattctttg cctaaacgtt atattccaaa aattgattac cgccggtctt ctcaaagcag ttagtttttc cgaagaaatc gcagacggaa atttaatcgc tcaaagttct cacgataaaa aagacgaaat cggaactctt ctcagttcca tgaataaaat gagggaaaat cttctcaaag ttctgagaga aatagggggt tcagcaaaca ctctcagaga tacttcagaa aaaatggccg attcatctag aaacttttct gacgtcgctc aaactcaagc ttctgctgct gaagaatctt ctgctgctgt cgaagaactc gccgcttccg cacaaaacgt gggaaagtct atggaaaagg ctgtattgag tatgaaagaa atcgatggta acgtcattcg tctaaaagaa caaatcgtaa atatcaatag agaaatgcaa gatctagttg agctcgcagc cctttcaaaa gaacaaggtg tcactggaga aaatgctatg atcgcttcta caagcgctat ggcggctatc ggagacagcg cttccagaat cactgaaatc ctttctataa ttactgaaat ttcggaaaga acaaatctac ttgctctcaa tgccgctatc gaagccgcta gagctggtga agccggaaaa ggatttgctg ttgtcgcaga agaaattgga aaactcgctt ctcaaacttc tacaagtgtt caagaaattg ggtctctggt gaattccact aataccgccg ttttaaatgg aaacactaaa gttgcagaag cttttaacat tctaaaaaaa cttagagaac aagtagaaga attcgatcgt tatgctaaga acgttcttac ttccgttaaa actcaggaag aaaatactaa agaaatcgca caaagtgcta atgaacttat gaccttcagt ttacaaattg aagaagctgt tctagaacaa agacgtgcca cggatgaaat tacaaaaacg atcatgagca tttctgacgg tactcaagaa atcgcttcag gtgcagaaga tcttacttca ttctccggaa atatgcacgg acaagctcaa aatctaggaa aattgatcgg taagtttaaa atcaactaa
Details for LpinA.18975.a.B2.GE42898
| HARVESTED ON: | 2/7/2019 |
| SEQUENCED ON: | 2/8/2019 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHME ITAERWTYEV KDYLDTGMGI IRGFRFPLLF SAPPRNQIIA ALREILKVND HYFGARLAYE PNSLDGNDLE FQNTLGHDST GRFIPYLHRG QTKEEIVLEX AKYYDSLGPE GDWYQVPKKT KSHYATDPYY YEIKGKVKIL MMSLMVPLYV NDQFYGVAGL DYQLEELQQR IGVKKPFQDL GYLTLISPKG IYAVNGFDSN RVGEKISDAK ELEYYLSKSQ EGEKFTTDSD GYTHYYFPFH IGKDKRYWVM QVSIPNSIYK E
Validated NT Sequence
atggctcacc accaccacca ccatatggaa attacagccg aacgttggac ctatgaagtg aaagattatt tagacactgg tatgggtatc atacgaggtt ttcggtttcc tcttctcttt agtgcccctc cccgcaatca aatcattgct gctttaagag aaatcttaaa agtcaatgat cattactttg gtgccagact cgcctacgaa ccaaattctt tggacggaaa cgacttagaa tttcaaaata ctctaggaca tgattccaca ggcagattta ttccgtatct tcatcgtggt caaacaaagg aagaaatcgt tctcgaagmc gctaaatact acgattcttt aggtccggaa ggagattggt accaagttcc taaaaaaaca aaatctcact acgcaacgga tccgtactat tatgaaatca aagggaaagt aaaaattcta atgatgtctc ttatggttcc gctttatgta aacgatcagt tttacggtgt tgccggttta gactatcaat tggaagaact tcaacaacga atcggcgtta aaaaaccgtt tcaagatctt ggttatctaa ctctgatttc tcctaaagga atttatgcag taaatggttt tgattcaaat cgggtcggtg aaaaaatttc agatgcaaaa gaactagaat attaccttag taaaagtcag gaaggcgaaa aattcactac tgattccgac ggatacactc attattactt tccgtttcat attggtaaag acaaacgcta ttgggtaatg caagtaagta ttccaaattc tatctataaa gaataa
Expected Protein Sequence
MAHHHHHHEI TAERWTYEVK DYLDTGMGII RGFRFPLLFS APPRNQIIAA LREILKVNDH YFGARLAYEP NSLDGNDLEF QNTLGHDSTG RFIPYLHRGQ TKEEIVLEAA KYYDSLGPEG DWYQVPKKTK SHYATDPYYY EIKGKVKILM MSLMVPLYVN DQFYGVAGLD YQLEELQQRI GVKKPFQDLG YLTLISPKGI YAVNGFDSNR VGEKISDAKE LEYYLSKSQE GEKFTTDSDG YTHYYFPFHI GKDKRYWVMQ VSIPNSIYKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaatta cagccgaacg ttggacctat gaagtgaaag attatttaga cactggtatg ggtatcatac gaggttttcg gtttcctctt ctctttagtg cccctccccg caatcaaatc attgctgctt taagagaaat cttaaaagtc aatgatcatt actttggtgc cagactcgcc tacgaaccaa attctttgga cggaaacgac ttagaatttc aaaatactct aggacatgat tccacaggca gatttattcc gtatcttcat cgtggtcaaa caaaggaaga aatcgttctc gaagccgcta aatactacga ttctttaggt ccggaaggag attggtacca agttcctaaa aaaacaaaat ctcactacgc aacggatccg tactattatg aaatcaaagg gaaagtaaaa attctaatga tgtctcttat ggttccgctt tatgtaaacg atcagtttta cggtgttgcc ggtttagact atcaattgga agaacttcaa caacgaatcg gcgttaaaaa accgtttcaa gatcttggtt atctaactct gatttctcct aaaggaattt atgcagtaaa tggttttgat tcaaatcggg tcggtgaaaa aatttcagat gcaaaagaac tagaatatta ccttagtaaa agtcaggaag gcgaaaaatt cactactgat tccgacggat acactcatta ttactttccg tttcatattg gtaaagacaa acgctattgg gtaatgcaag taagtattcc aaattctatc tataaagaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LpinA.18975.a.B3.GE41957
| HARVESTED ON: | 10/3/2017 |
| SEQUENCED ON: | 10/12/2017 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Insoluble |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMI TAGLLKAVSF SEEIADGNLI AQSSHDKKDE IGTLLSSMNK MRENLLKVLR EIGGSANTLR DTSEKMADSS RNFSDVAQTQ ASAAEESSAA VEELAASAQN VGKSMEKAVL SMKEIDGNVI RLKEQIVNIN REMQDLVELA ALSKEQGVTG ENAMIASTSA MAAIGDSASR ITEILSIITE ISERTNLLAL NAAIEAARAG EAGKGFAVVA EEIGKLASQT STSVQEIGSL VNSTNTAVLN GNTKVAEAFN ILKKLREQVE EFDRYAKNVL TSVKTQEENT KEIAQSANEL MTFSLQIEEA VLEQRRATDE ITKTIMSISD GTQEIASGAE DLTSFSGNMH GQAQNLGK
Validated NT Sequence
aattttccta gattttgagc ttgtccgtgc atatttccgg agaatgaagt aagatcttct gcacctgaag cgatttcttg agtaccgtca gaaatgctca tgatcgtttt tgtaatttca tccgtggcac gtctttgttc tagaacagct tcttcaattt gtaaactgaa ggtcataagt tcattagcac tttgtgcgat ttctttagta ttttcttcct gagttttaac ggaagtaaga acgttcttag cataacgatc gaattcttct acttgttctc taagtttttt tagaatgtta aaagcttctg caactttagt gtttccattt aaaacggcgg tattagtgga attcaccaga gacccaattt cttgaacact tgtagaagtt tgagaagcga gttttccaat ttcttctgcg acaacagcaa atccttttcc ggcttcacca gctctagcgg cttcgatagc ggcattgaga gcaagtagat ttgttctttc cgaaatttca gtaattatag aaaggatttc agtgattctg gaagcgctgt ctccgatagc cgccatagcg cttgtagaag cgatcatagc attttctcca gtgacacctt gttcttttga aagggctgcg agctcaacta gatcttgcat ttctctattg atatttacga tttgttcttt tagacgaatg acgttaccat cgatttcttt catactcaat acagcctttt ccatagactt tcccacgttt tgtgcggaag cggcgagttc ttcgacagca gcagaagatt cttcagcagc agaagcttga gtttgagcga cgtcagaaaa gtttctagat gaatcggcca ttttttctga agtatctctg agagtgtttg ctgaaccccc tatttctctc agaactttga gaagattttc cctcatttta ttcatggaac tgagaagagt tccgatttcg tcttttttat cgtgagaact ttgagcgatt aaatttccgt ctgcgatttc ttcggaaaaa ctaactgctt tgagaagacc ggcggtaatc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHIT AGLLKAVSFS EEIADGNLIA QSSHDKKDEI GTLLSSMNKM RENLLKVLRE IGGSANTLRD TSEKMADSSR NFSDVAQTQA SAAEESSAAV EELAASAQNV GKSMEKAVLS MKEIDGNVIR LKEQIVNINR EMQDLVELAA LSKEQGVTGE NAMIASTSAM AAIGDSASRI TEILSIITEI SERTNLLALN AAIEAARAGE AGKGFAVVAE EIGKLASQTS TSVQEIGSLV NSTNTAVLNG NTKVAEAFNI LKKLREQVEE FDRYAKNVLT SVKTQEENTK EIAQSANELM TFSLQIEEAV LEQRRATDEI TKTIMSISDG TQEIASGAED LTSFSGNMHG QAQNLGKLIG KFKIN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattaccg ccggtcttct caaagcagtt agtttttccg aagaaatcgc agacggaaat ttaatcgctc aaagttctca cgataaaaaa gacgaaatcg gaactcttct cagttccatg aataaaatga gggaaaatct tctcaaagtt ctgagagaaa tagggggttc agcaaacact ctcagagata cttcagaaaa aatggccgat tcatctagaa acttttctga cgtcgctcaa actcaagctt ctgctgctga agaatcttct gctgctgtcg aagaactcgc cgcttccgca caaaacgtgg gaaagtctat ggaaaaggct gtattgagta tgaaagaaat cgatggtaac gtcattcgtc taaaagaaca aatcgtaaat atcaatagag aaatgcaaga tctagttgag ctcgcagccc tttcaaaaga acaaggtgtc actggagaaa atgctatgat cgcttctaca agcgctatgg cggctatcgg agacagcgct tccagaatca ctgaaatcct ttctataatt actgaaattt cggaaagaac aaatctactt gctctcaatg ccgctatcga agccgctaga gctggtgaag ccggaaaagg atttgctgtt gtcgcagaag aaattggaaa actcgcttct caaacttcta caagtgttca agaaattggg tctctggtga attccactaa taccgccgtt ttaaatggaa acactaaagt tgcagaagct tttaacattc taaaaaaact tagagaacaa gtagaagaat tcgatcgtta tgctaagaac gttcttactt ccgttaaaac tcaggaagaa aatactaaag aaatcgcaca aagtgctaat gaacttatga ccttcagttt acaaattgaa gaagctgttc tagaacaaag acgtgccacg gatgaaatta caaaaacgat catgagcatt tctgacggta ctcaagaaat cgcttcaggt gcagaagatc ttacttcatt ctccggaaat atgcacggac aagctcaaaa tctaggaaaa ttgatcggta agtttaaaat caactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat