SSGCID: Target Page

LpinA.18694.a

Acriflavin resistance

CENTER ID:	LpinA.18694.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.18694.a.B2.GE42897	soluble domain #1		32	338
LpinA.18694.a.B3.GE41955	soluble domain #2		612	907

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.3720
UniProt:	Q72MW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLASLSIQR PIFVTCTVLL ILVAGYLSLS KLGVDLFPNV TIPVVTVTVP YPGAAPNEIE TLIAKPVEDE LSTISGVKRV RAVCNEGVGT VIVEFTLETD VKYAEQQVRD KVSSVKPKLP NDAKEPVIRR IDPADQPIVI IALRAELPDA ELYDIANEEV KQILLSTKDV GNVTIYGGRK REIHVELDRN KLKEHMIPAS VVSNRLASGG MNIPAGKVSK TDKELVYRTI NEFQSPEEIR DTPISLFGNE VPIKIGQLGE VKDTVEDETS RAYLNGRKAV FLMVYKQSGS NTVAVAQSVK KKVLEINQDL SKRNGSPELT STNDSSVTID NNIYDVKETI IIGIILTIIV VLLFLGSVRS TLITGLALPN SLLGAFILMA IAGFTVNVMT LLALSLAVGL LIDDAIVVRE NIFRHREMGK TAREASIEGT KEVTLAVIAT TMTVIAVFMP IAFISGIVGQ FLREFGLTVC FALLISLYDA LTIAPMLSAY FGGKVGNHAH NSSESIPEIT TKAVKGKTKG ATTTLEEIAY SKIRSQNKTS RGILSTVFSP IVFILGKLEV GLDSILSIFN VFQSWLEEKY ASVLKFTLKR PFFILSGAIL IFVVSLVLTK FIPKTFLPAQ DEGKFTVTLD MPPGTSLEKM SQIALQVDQK IRSYKEIKIV SMFNTNRSTN MFVEMVPSRD RKMNTTQFKA FLRNELAEFS YANPIVKDVD NVGGGQRPFT LTVSGQKREV VEDYAKKLFE RLQKSPALLD VDTSYRTGAP EFRVVPDREK EVLLGVPGTI IGTELRTLVE GTTPAVYREN GVEYDIRVRL KDSQRDLKEN FYSSFVPNFN NRLIPIQNVA KAEESTGLAT INRLNRNKAV EIYADVNPKG PEMGGAMEEV TKISQIEIPL PSGVKIGYSG QAESFKEMGV SMAIAMGLGI LFIYMVLASL YESFITPIAI MLVLPLALCG AFIALFITQK SLDIFSMIGL IMLIGVATKN SILLVDFTNQ LLSQGKEMKE AIIEAGRERL RPILMTSFAL IAGMLPIAIG LNEASRQRTS MGVAIIGGLI SSTILTLVVV PAAFSYIEKL NQMVRRNSPN PDA

NT Sequence

atgaacttag cttcgctttc gattcaaaga ccgattttcg tcacttgtac ggtcttactc atcctggtcg cgggatacct ttctttgagc aaattggggg tagatttatt tcccaatgta acgattccgg tcgtgaccgt aaccgtgcct tatccaggtg cggcgcctaa tgaaatcgaa acactaattg caaaaccggt tgaagacgaa ctatctacga tttcgggagt gaaaagggtc agagcggtct gtaacgaagg agtggggacc gttattgtag agtttactct ggaaactgat gtaaagtatg cagaacaaca ggttcgagat aaagtatcta gtgttaaacc gaaattacca aatgatgcaa aagaaccagt cattcgaaga atcgatccag cggaccaacc gatcgtaatc attgcactaa gagctgaatt accagatgcg gaactttatg atattgctaa tgaagaagtg aagcagattc ttctttctac caaagacgta ggaaacgtaa ccatctacgg aggacgtaaa agagaaattc acgtagaact ggatcgaaac aaactcaaag aacatatgat tccagcttca gttgtgtcta atcgacttgc ttcgggagga atgaacatcc ccgccggaaa agtaagtaaa accgataagg aacttgtata tagaacgatc aatgaattcc aatctccaga ggaaatcaga gacactccta tctctctttt tggaaatgaa gttcctatta aaattggaca attgggcgaa gtcaaagata cagttgaaga cgaaacctct cgcgcttatc taaacggtcg aaaagccgtt tttctcatgg tatataaaca gtccgggtcc aatacggttg cggttgcaca atctgtaaaa aagaaagtct tagaaatcaa tcaagatctt tcgaaacgta acggttcacc tgagttgacg tctacgaatg attcttctgt aacgatcgac aacaatatct atgatgtaaa agaaacgatt atcattggaa tcatacttac gatcatcgta gtgttattat tcttaggaag tgttcgttct actttgatta ctggacttgc acttccaaac tctttgttag gcgcgtttat tttgatggcg attgctggtt ttaccgtaaa cgtgatgact cttttggctc tcagccttgc ggttggactt cttatcgacg atgcgattgt agttcgagaa aatattttca gacacagaga aatgggaaaa actgcgagag aggcttctat cgaaggcacc aaagaagtaa cacttgcggt aattgctact acgatgacgg tgattgcggt ttttatgccg attgcattta tcagcggaat tgtgggacag ttcttaagag aatttggatt gactgtatgt tttgctcttc tcatttctct ttatgacgcg cttacgattg ctcctatgtt gtctgcatat tttggtggaa aggtaggaaa tcacgcacat aattcttccg aatcgattcc tgaaatcaca accaaagcag tcaaaggaaa aacaaaaggc gctactacta cgctggaaga gattgcttat tctaaaatcc gttctcagaa taaaacttcc agaggaattt tatccaccgt attttctcct attgtgttca ttttaggaaa gttagaagta ggactagatt ctatattaag tatttttaat gtttttcaat cttggttaga agaaaaatac gcttccgttt taaaattcac tttaaaaaga ccttttttta tactttctgg agcgatttta attttcgtag ttagtttggt tttgactaag tttattccaa aaacgtttct tcctgctcag gatgagggta aatttacagt gactttggat atgccacccg gaacgtcctt ggaaaaaatg tctcagattg ctcttcaagt agaccaaaag attcgttctt ataaggaaat caaaattgtt tccatgttta acacgaaccg tagtacgaat atgttcgtag aaatggttcc ttccagagat aggaagatga acacaactca gtttaaagcg tttcttcgta atgaattggc ggaattttct tatgccaatc cgatcgtaaa ggacgtagac aatgtgggag gaggacaaag accttttact ctaacagtca gcggtcaaaa gagagaagtt gtagaagact atgctaagaa gttgttcgaa cgtcttcaaa aatctccggc gcttttagac gtagatacga gttatcgaac gggagctcct gagtttagag tggttccgga tcgagaaaag gaagtacttt taggagttcc tggaacgatt ataggaactg aattgagaac tcttgtagaa ggaactacac ctgcggttta tagagaaaat ggagttgagt atgatattcg agttcgactg aaggattctc aaagagattt aaaggaaaat ttttatagtt cttttgtacc taactttaac aatagattga ttccaattca aaacgtagca aaggcggaag aatccactgg tcttgcaacg atcaatcgtc tcaaccgaaa taaggccgtg gaaatttatg cagatgtaaa tccaaaaggt cctgaaatgg gaggagctat ggaagaagtt acaaagatca gtcagattga aattcctctt ccttccggtg taaagatcgg ctattctgga caggcagaaa gttttaaaga gatgggagtt tctatggcga tcgccatggg acttgggatt ttatttatct acatggttct tgcttctctt tacgaaagtt ttatcactcc gattgcgatc atgctcgtat tacctcttgc gctttgtgga gcttttatcg ctctttttat tacccaaaaa tccttggata ttttttctat gattgggctg attatgttga tcggtgtcgc taccaagaac tcaattcttc ttgtggactt tacgaatcaa ttgttgtcac aaggtaaaga aatgaaagaa gcgattatcg aagcgggaag agaaagattg agaccgattt tgatgacttc ttttgctctt atcgcgggaa tgttaccgat tgcaatcggg ttgaacgaag cttctcgtca aagaacgagt atgggagttg cgatcattgg tggattgatt tcttctacga tacttacttt agtggtggtt cctgcagcat tttcttatat agaaaaactg aatcaaatgg taagaagaaa ttctccgaat cctgatgcgt aa

Details for LpinA.18694.a.B2.GE42897

HARVESTED ON:	2/7/2019
SEQUENCED ON:	2/8/2019
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHLG VDLFPNVTIP VVTVTVPYPG AAPNEIETLI AKPVEDELST ISGVKRVRAV CNEGVGTVIV EFTLETDVKY AEQQVRDKVS SVKPKLPNDA KEPVIRRIDP ADQPIVIIAL RAELPDAELY DIANEEVKQI LLSTKDVGNV TIYGGRKREI HVELDRNKLK EHMIPASVVS NRLASGGMNI PAGKVSKTDK ELVYRTINEF QSPEEIRDTP ISLFGNEVPI KIGQLGEVKD TVEDETSRAY LNGRKAVFLM VYKQSGSNTV AVAQSVKKKV LEINQDLSKR NGSPELTSTN DSSVTIDNNI YDVKE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggggg tagatttatt tcccaatgta acgattccgg tcgtgaccgt aaccgtgcct tatccaggtg cggcgcctaa tgaaatcgaa acactaattg caaaaccggt tgaagacgaa ctatctacga tttcgggagt gaaaagggtc agagcggtct gtaacgaagg agtggggacc gttattgtag agtttactct ggaaactgat gtaaagtatg cagaacaaca ggttcgagat aaagtatcta gtgttaaacc gaaattacca aatgatgcaa aagaaccagt cattcgaaga atcgatccag cggaccaacc gatcgtaatc attgcactaa gagctgaatt accagatgcg gaactttatg atattgctaa tgaagaagtg aagcagattc ttctttctac caaagacgta ggaaacgtaa ccatctacgg aggacgtaaa agagaaattc acgtagaact ggatcgaaac aaactcaaag aacatatgat tccagcttca gttgtgtcta atcgacttgc ttcgggagga atgaacatcc ccgccggaaa agtaagtaaa accgataagg aacttgtata tagaacgatc aatgaattcc aatctccaga ggaaatcaga gacactccta tctctctttt tggaaatgaa gttcctatta aaattggaca attgggcgaa gtcaaagata cagttgaaga cgaaacctct cgcgcttatc taaacggtcg aaaagccgtt tttctcatgg tatataaaca gtccgggtcc aatacggttg cggttgcaca atctgtaaaa aagaaagtct tagaaatcaa tcaagatctt tcgaaacgta acggttcacc tgagttgacg tctacgaatg attcttctgt aacgatcgac aacaatatct atgatgtaaa agaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LpinA.18694.a.B3.GE41955

HARVESTED ON:	10/3/2017
SEQUENCED ON:	10/12/2017
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI PKTFLPAQDE GKFTVTLDMP PGTSLEKMSQ IALQVDQKIR SYKEIKIVSM FNTNRSTNMF VEMVPSRDRK MNTTQFKAFL RNELAEFSYA NPIVKDVDNV GGGQRPFTLT VSGQKREVVE DYAKKLFERL QKSPALLDVD TSYRTGAPEF RVVPDREKEV LLGVPGTIIG TELRTLVEGT TPAVYRENGV EYDIRVRLKD SQRDLKENFY SSFVPNFNNR LIPIQNVAKA EESTGLATIN RLNRNKAVEI YADVNPKGPE MGGAMEEVTK ISQIEIPLPS GVKIGYXXQA ESFKE

Validated NT Sequence

ttactcactt actctttaaa actttctgcc tgtnnnnnat agccgatctt tacaccggaa ggaagaggaa tttcaatctg actgatcttt gtaacttctt ccatagctcc tcccatttca ggaccttttg gatttacatc tgcataaatt tccacggcct tatttcggtt gagacgattg atcgttgcaa gaccagtgga ttcttccgcc tttgctacgt tttgaattgg aatcaatcta ttgttaaagt taggtacaaa agaactataa aaattttcct ttaaatctct ttgagaatcc ttcagtcgaa ctcgaatatc atactcaact ccattttctc tataaaccgc aggtgtagtt ccttctacaa gagttctcaa ttcagttcct ataatcgttc caggaactcc taaaagtact tccttttctc gatccggaac cactctaaac tcaggagctc ccgttcgata actcgtatct acgtctaaaa gcgccggaga tttttgaaga cgttcgaaca acttcttagc atagtcttct acaacttctc tcttttgacc gctgactgtt agagtaaaag gtctttgtcc tcctcccaca ttgtctacgt cctttacgat cggattggca taagaaaatt ccgccaattc attacgaaga aacgctttaa actgagttgt gttcatcttc ctatctctgg aaggaaccat ttctacgaac atattcgtac tacggttcgt gttaaacatg gaaacaattt tgatttcctt ataagaacga atcttttggt ctacttgaag agcaatctga gacatttttt ccaaggacgt tccgggtggc atatccaaag tcactgtaaa tttaccctca tcctgagcag gaagaaacgt ttttggaatc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHIP KTFLPAQDEG KFTVTLDMPP GTSLEKMSQI ALQVDQKIRS YKEIKIVSMF NTNRSTNMFV EMVPSRDRKM NTTQFKAFLR NELAEFSYAN PIVKDVDNVG GGQRPFTLTV SGQKREVVED YAKKLFERLQ KSPALLDVDT SYRTGAPEFR VVPDREKEVL LGVPGTIIGT ELRTLVEGTT PAVYRENGVE YDIRVRLKDS QRDLKENFYS SFVPNFNNRL IPIQNVAKAE ESTGLATINR LNRNKAVEIY ADVNPKGPEM GGAMEEVTKI SQIEIPLPSG VKIGYSGQAE SFKE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattccaa aaacgtttct tcctgctcag gatgagggta aatttacagt gactttggat atgccacccg gaacgtcctt ggaaaaaatg tctcagattg ctcttcaagt agaccaaaag attcgttctt ataaggaaat caaaattgtt tccatgttta acacgaaccg tagtacgaat atgttcgtag aaatggttcc ttccagagat aggaagatga acacaactca gtttaaagcg tttcttcgta atgaattggc ggaattttct tatgccaatc cgatcgtaaa ggacgtagac aatgtgggag gaggacaaag accttttact ctaacagtca gcggtcaaaa gagagaagtt gtagaagact atgctaagaa gttgttcgaa cgtcttcaaa aatctccggc gcttttagac gtagatacga gttatcgaac gggagctcct gagtttagag tggttccgga tcgagaaaag gaagtacttt taggagttcc tggaacgatt ataggaactg aattgagaac tcttgtagaa ggaactacac ctgcggttta tagagaaaat ggagttgagt atgatattcg agttcgactg aaggattctc aaagagattt aaaggaaaat ttttatagtt cttttgtacc taactttaac aatagattga ttccaattca aaacgtagca aaggcggaag aatccactgg tcttgcaacg atcaatcgtc tcaaccgaaa taaggccgtg gaaatttatg cagatgtaaa tccaaaaggt cctgaaatgg gaggagctat ggaagaagtt acaaagatca gtcagattga aattcctctt ccttccggtg taaagatcgg ctattctgga caggcagaaa gttttaaaga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat