LpinA.18138.a

Diguanylate phosphodiesterase

CENTER ID: LpinA.18138.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.18138.a.B1.GE41896 full length 1 442
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.777
UniProt: Q72UL6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSPVSRISV KGFLNKYMLY SQDTSNLLSY GENNYQPHYQ PILEVTNCNI IGYEVLGRFY FPEKNEYRSL GYQFHNPELD AIRLIQIDRL IREKAIRHLK DSGLRTKLFL NMMPNFLSMI HTGDVLDLKR IHVLNLIEKY DIPPSEVVLE ITEDKFDGNI EKLLSIVNVF KDYGFKIAVD DLGVGFSNLE RIGYIHPDIM KVDIKIMRES LNRRSFKNVL SAIADMSQKL GSDLLFEGIE TEEELHLALS MGANLLQGFY FSRPQVEFQD KKQFNRTLRD TLEKFSGLRF MELLEEFQKG QSVIDSLGEK LEALRHNGKE DLPLVLHLML SELPTEILSV FACDIFGYQI TPTYFRVHPG EEWDSDLTEI GNNYAWRPFF IRHKAKVVQN AAKWTVTEPM YDMDLHKQVV IFTYTLRDNY ILVIKMDWEK EGGGLKTEDR RQ
NT Sequence
ttgcggagcc cggtttcgcg gatttcagta aaaggatttt taaataagta catgttgtat agccaggaca caagcaattt actctcttat ggagaaaaca attatcaacc tcattaccag ccgattctgg aggtaactaa ttgtaacatt ataggatatg aagttctagg aagattttat tttcccgaaa agaatgaata ccgttctttg ggctatcagt ttcacaatcc tgaactagat gcaattcgac tgattcaaat cgatcgattg attcgagaaa aagcgatccg acatctcaaa gactccggtt tacgaacaaa acttttcctc aacatgatgc ctaattttct atctatgatt catacaggag atgtattgga tctcaaaaga attcacgttc tcaatctgat cgaaaagtat gatattccgc cttctgaagt ggttttagaa atcacggaag ataagtttga cggtaatata gaaaaacttc tttcgattgt taatgtgttc aaagactacg gatttaaaat tgcggtggat gatttaggag ttgggttttc caatttggaa agaatcggtt atattcatcc tgatataatg aaggtggaca tcaagatcat gcgggaaagt ttaaaccgaa gatcttttaa gaacgtattg agtgcgattg cagacatgtc tcaaaaacta ggttctgatc ttctatttga aggaattgaa acggaggaag aattacatct tgctctttct atgggggcga atcttctcca gggattttat ttttcaagac ctcaagtaga gtttcaggat aaaaaacaat tcaatagaac tctcagagat actttggaaa aattctctgg acttcggttt atggaacttt tagaggaatt tcaaaaaggt cagtcggtta tagattcctt gggggaaaaa ttagaagctc ttcgacataa tggaaaagaa gatcttcctt tggttcttca tctgatgctc tcggaacttc ccacggaaat tctttccgta tttgcctgtg atatttttgg ttatcagatt actcccactt attttcgagt tcatcctggc gaagagtggg attccgatct tacggaaatt ggaaacaatt atgcctggag accttttttc atccgtcata aagcgaaggt ggttcagaat gctgcaaaat ggactgtaac cgaaccaatg tatgatatgg atcttcacaa acaagttgtg atctttactt atactctcag agataattat attttagtaa ttaagatgga ttgggaaaag gaaggcggag gattgaagac tgaggataga agacaataa
Details for LpinA.18138.a.B1.GE41896
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML RSPVSRISVK GFLNKYMLYS QDTSNLLSYG ENNYQPHYQP ILEVTNCNII GYEVLGRFYF PEKNEYRSLG YQFHNPELDA IRLIQIDRLI REKAIRHLKD SGLRTKLFLN MMPNFLSMIH TGDVLDLKRI HVLNLIEKYD IPPSEVVLEI TEDKFDGNIE KLLSIVNVFK DYGFKIAVDD LGVGFSNLER IGYIHPDIMK VDIKIMRESL NRRSFKNVLS AIADMSQKLG SDLLFEGIET EEELHLALSM GANLLQGFYF SRPQVEFQDK KQFNRTLRDT LEKFSGLRFM ELLEEFQKGQ SVIDSLGEKL EALRHNGKED LPLVLHLMLS ELPTEILSVF ACDIFGYQIT PTYFRVHPGE EWDSDLTEIG NNYAWRPFFI RHKAKVVQNA AKWTVTEPMY DMDLHKQVVI FTYTLRDNYI LVIKMDWEKE GG
Validated NT Sequence
ctccgccttc cttttcccaa tccatcttaa ttactaaaat ataattatct ctgagagtat aagtaaagat cacaacttgt ttgtgaagat ccatatcata cattggttcg gttacagtcc attttgcagc attctgaacc accttcgctt tatgacggat gaaaaaaggt ctccaggcat aattgtttcc aatttccgta agatcggaat cccactcttc gccaggatga actcgaaaat aagtgggagt aatctgataa ccaaaaatat cacaggcaaa tacggaaaga atttccgtgg gaagttccga gagcatcaga tgaagaacca aaggaagatc ttcttttcca ttatgtcgaa gagcttctaa tttttccccc aaggaatcta taaccgactg acctttttga aattcctcta aaagttccat aaaccgaagt ccagagaatt tttccaaagt atctctgaga gttctattga attgtttttt atcctgaaac tctacttgag gtcttgaaaa ataaaatccc tggagaagat tcgcccccat agaaagagca agatgtaatt cttcctccgt ttcaattcct tcaaatagaa gatcagaacc tagtttttga gacatgtctg caatcgcact caatacgttc ttaaaagatc ttcggtttaa actttcccgc atgatcttga tgtccacctt cattatatca ggatgaatat aaccgattct ttccaaattg gaaaacccaa ctcctaaatc atccaccgca attttaaatc cgtagtcttt gaacacatta acaatcgaaa gaagtttttc tatattaccg tcaaacttat cttccgtgat ttctaaaacc acttcagaag gcggaatatc atacttttcg atcagattga gaacgtgaat tcttttgaga tccaatacat ctcctgtatg aatcatagat agaaaattag gcatcatgtt gaggaaaagt tttgttcgta aaccggagtc tttgagatgt cggatcgctt tttctcgaat caatcgatcg atttgaatca gtcgaattgc atctagttca ggattgtgaa actgatagcc caaagaacgg tattcattct tttcgggaaa ataaaatctt cctagaactt catatcctat aatgttacaa ttagttacct ccagaatcgg ctggtaatga ggttgataat tgttttctcc ataagagagt aaattgcttg tgtcctggct atacaacatg tacttattta aaaatccttt tactgaaatc cgcgaaaccg ggctccgcaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMR SPVSRISVKG FLNKYMLYSQ DTSNLLSYGE NNYQPHYQPI LEVTNCNIIG YEVLGRFYFP EKNEYRSLGY QFHNPELDAI RLIQIDRLIR EKAIRHLKDS GLRTKLFLNM MPNFLSMIHT GDVLDLKRIH VLNLIEKYDI PPSEVVLEIT EDKFDGNIEK LLSIVNVFKD YGFKIAVDDL GVGFSNLERI GYIHPDIMKV DIKIMRESLN RRSFKNVLSA IADMSQKLGS DLLFEGIETE EELHLALSMG ANLLQGFYFS RPQVEFQDKK QFNRTLRDTL EKFSGLRFME LLEEFQKGQS VIDSLGEKLE ALRHNGKEDL PLVLHLMLSE LPTEILSVFA CDIFGYQITP TYFRVHPGEE WDSDLTEIGN NYAWRPFFIR HKAKVVQNAA KWTVTEPMYD MDLHKQVVIF TYTLRDNYIL VIKMDWEKEG GGLKTEDRRQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcgga gcccggtttc gcggatttca gtaaaaggat ttttaaataa gtacatgttg tatagccagg acacaagcaa tttactctct tatggagaaa acaattatca acctcattac cagccgattc tggaggtaac taattgtaac attataggat atgaagttct aggaagattt tattttcccg aaaagaatga ataccgttct ttgggctatc agtttcacaa tcctgaacta gatgcaattc gactgattca aatcgatcga ttgattcgag aaaaagcgat ccgacatctc aaagactccg gtttacgaac aaaacttttc ctcaacatga tgcctaattt tctatctatg attcatacag gagatgtatt ggatctcaaa agaattcacg ttctcaatct gatcgaaaag tatgatattc cgccttctga agtggtttta gaaatcacgg aagataagtt tgacggtaat atagaaaaac ttctttcgat tgttaatgtg ttcaaagact acggatttaa aattgcggtg gatgatttag gagttgggtt ttccaatttg gaaagaatcg gttatattca tcctgatata atgaaggtgg acatcaagat catgcgggaa agtttaaacc gaagatcttt taagaacgta ttgagtgcga ttgcagacat gtctcaaaaa ctaggttctg atcttctatt tgaaggaatt gaaacggagg aagaattaca tcttgctctt tctatggggg cgaatcttct ccagggattt tatttttcaa gacctcaagt agagtttcag gataaaaaac aattcaatag aactctcaga gatactttgg aaaaattctc tggacttcgg tttatggaac ttttagagga atttcaaaaa ggtcagtcgg ttatagattc cttgggggaa aaattagaag ctcttcgaca taatggaaaa gaagatcttc ctttggttct tcatctgatg ctctcggaac ttcccacgga aattctttcc gtatttgcct gtgatatttt tggttatcag attactccca cttattttcg agttcatcct ggcgaagagt gggattccga tcttacggaa attggaaaca attatgcctg gagacctttt ttcatccgtc ataaagcgaa ggtggttcag aatgctgcaa aatggactgt aaccgaacca atgtatgata tggatcttca caaacaagtt gtgatcttta cttatactct cagagataat tatattttag taattaagat ggattgggaa aaggaaggcg gaggattgaa gactgaggat agaagacaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat