SSGCID: Target Page

LpinA.18064.a

Gamma-glutamyltranspeptidase

CENTER ID:	LpinA.18064.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.18064.a.B2.GE42895	mature protein		27	577

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.18064.a.B2.PW38638	mature protein		27	577

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.2177
UniProt:	Q72RD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQITIRIFL ILLLFSCREN SLLIEGKKIS TDLLVVPSHQ KTHTDYFSES KNLMISSDSP EATQAGIEVG ALGGNVVDVV VATSFAISVT RPHSTGLGGG GFLILYLKEF PEPIAFDFRE RAPNTSSRDM YKLKPKEDSL LGFRAVGVPG NVAGLVQIQR RFGKLPLKTV ISPSIRLAEN GFPVYPDLQS AIQKSSKDMS EEMKNIFLPK GKIPELNSIL VQKDLANSLR LISETGDKEF YHGKIANSIV NAMKKNDGLI TLQDLSGYKV IEKKTVHTTY HDYTIYTMPP PSSGVHLLTM LSMVETKPLK ETYEKDPVLY YHFIVEAMRR GYADRAMLGG DPAFTKIPIE RLVSKKYAEE KISNFNPKTA SSSSSFLKTL NFGVESPQTT HISVMDREGN SVSTTQSINF RFGASVVAPG TGIVLNDTMD DFSRAPGEPN VYGLIGAEAN SILPKKTPLS SMSPTIVFKN KEPFLVTGAP GGSYIVNAVL QSLIYNLDFN LTLYESVARG RIHHQFFPDA VFIEKSVNER NVFDGLSSKK HDLRIAPNFA KLFSVKRENG MLYGACDPRG EGATGGL

NT Sequence

atgaaacaaa ttacaattcg tatctttttg atacttttac tcttctcttg tagggaaaat tctcttctga ttgaggggaa aaaaatttcc accgatctgc tcgtagttcc ttcccaccaa aaaacgcata ctgattactt tagtgaaagt aaaaatttaa tgatatcctc cgattctcct gaggcgacac aggccgggat cgaagtaggc gcgttaggcg gaaatgtagt ggacgtagta gtagcgactt cttttgcaat ttctgttaca agaccccatt ctactggttt gggaggagga ggttttttaa ttctttattt aaaagaattt ccagaaccga tcgctttcga ttttagagaa agggcgccga atacgtcttc tagagatatg tataaactaa aacctaagga agattctctt ttagggttta gggcagtagg tgttccgggt aacgttgcag ggcttgtgca gatccaaaga cgttttggaa aacttccgct taaaactgtg atctccccct cgatccgact cgccgagaat ggatttccag tttatccaga ccttcagtcc gcaattcaaa aatcttctaa agatatgagc gaagaaatga aaaatatctt tcttcccaaa ggaaaaattc cggagttaaa cagtattctc gttcaaaagg atcttgcaaa ttctctcaga ctcatttccg aaactggcga taaagagttt tatcacggta aaattgcgaa ttcgattgtc aatgcgatga aaaaaaacga tggtcttatc actctacaag atctgagcgg ttataaagta atcgagaaaa aaacagtaca tacaacttac cacgattata cgatttacac gatgcctcct ccttcttctg gagttcattt actgactatg ttgtctatgg tggaaacaaa acctctgaaa gagacgtatg aaaaagatcc tgttttatat tatcatttta ttgtagaagc gatgagaaga ggttatgcgg acagagcgat gttaggtgga gatcctgcct ttacaaaaat tccaattgaa cgtcttgttt ctaaaaaata cgcggaagaa aaaatttcaa attttaatcc taaaacagct tctagtagct cttctttttt aaaaacttta aactttggag tagaatctcc acaaactaca catatttccg ttatggatcg tgaaggaaat tcagtttcta caactcagtc tatcaatttt agatttggag cttctgtagt cgctcctgga actggaattg tgttaaacga tacaatggat gattttagtc gagctccagg agaaccaaat gtatacggtt taatcggtgc ggaagcaaat tctatacttc ctaagaaaac tcctttgagc agtatgtctc ctacaattgt atttaaaaat aaagaacctt ttttagttac tggtgctcct ggaggttctt atatcgtaaa cgccgtttta cagtctttga tctataattt agattttaat ctgactttgt atgaatccgt agccagggga agaatacatc atcaattttt tccggatgcg gtatttatag aaaaatcagt aaatgaaaga aacgtatttg atggtctttc ctctaaaaaa cacgatctta gaattgctcc gaactttgcg aaactatttt ctgtcaaaag agagaatgga atgctctatg gagcttgcga tccacgtgga gaaggggcca caggcggact ttga

Details for LpinA.18064.a.B2.GE42895

HARVESTED ON:	2/7/2019
SEQUENCED ON:	2/8/2019
EXPECTED MW:	61kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHKK ISTDLLVVPS HQKTHTDYFS ESKNLMISSD SPEATQAGIE VGALGGNVVD VVVATSFAIS VTRPHSTGLG GGGFLILYLK EFPEPIAFDF RERAPNTSSR DMYKLKPKED SLLGFRAVGV PGNVAGLVQI QRRFGKLPLK TVISPSIRLA ENGFPVYPDL QSAIQKSSKD MSEEMKNIFL PKGKIPELNS ILVQKDLANS LRLISETGDK EFYHGKIANS IVNAMKKNDG LITLQDLSGY KVIEKKTVHT TYHDYTIYTM PPPSSGVHLL TMLSMVETKP LKETYEKDPV LYYHFIVEAM RRGYADRAML GGDPAFTKIP IERLVSKKYA EEKISNFNPK TASSSSSFLK TLNFGVESPQ TTHISVMDRE GNSVSTTQSI NFRFGASVVA PGTGIVLNDT MDDFSRAPGE PNVYGLIGAE ANSILPKKTP LSSMSPTIVF KNKEPFLVTG APGGSYIVNA VLQSLIYNLD FNLTLYESVA RGRIHHQFFP DAVFIEKSVN ERNVFDGLSS KKHDLRIAPN FAKLFSVKRE NGMLYGACDP RGEGATGGL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaaaaaa tttccaccga tctgctcgta gttccttccc accaaaaaac gcatactgat tactttagtg aaagtaaaaa tttaatgata tcctccgatt ctcctgaggc gacacaggcc gggatcgaag taggcgcgtt aggcggaaat gtagtggacg tagtagtagc gacttctttt gcaatttctg ttacaagacc ccattctact ggtttgggag gaggaggttt tttaattctt tatttaaaag aatttccaga accgatcgct ttcgatttta gagaaagggc gccgaatacg tcttctagag atatgtataa actaaaacct aaggaagatt ctcttttagg gtttagggca gtaggtgttc cgggtaacgt tgcagggctt gtgcagatcc aaagacgttt tggaaaactt ccgcttaaaa ctgtgatctc cccctcgatc cgactcgccg agaatggatt tccagtttat ccagaccttc agtccgcaat tcaaaaatct tctaaagata tgagcgaaga aatgaaaaat atctttcttc ccaaaggaaa aattccggag ttaaacagta ttctcgttca aaaggatctt gcaaattctc tcagactcat ttccgaaact ggcgataaag agttttatca cggtaaaatt gcgaattcga ttgtcaatgc gatgaaaaaa aacgatggtc ttatcactct acaagatctg agcggttata aagtaatcga gaaaaaaaca gtacatacaa cttaccacga ttatacgatt tacacgatgc ctcctccttc ttctggagtt catttactga ctatgttgtc tatggtggaa acaaaacctc tgaaagagac gtatgaaaaa gatcctgttt tatattatca ttttattgta gaagcgatga gaagaggtta tgcggacaga gcgatgttag gtggagatcc tgcctttaca aaaattccaa ttgaacgtct tgtttctaaa aaatacgcgg aagaaaaaat ttcaaatttt aatcctaaaa cagcttctag tagctcttct tttttaaaaa ctttaaactt tggagtagaa tctccacaaa ctacacatat ttccgttatg gatcgtgaag gaaattcagt ttctacaact cagtctatca attttagatt tggagcttct gtagtcgctc ctggaactgg aattgtgtta aacgatacaa tggatgattt tagtcgagct ccaggagaac caaatgtata cggtttaatc ggtgcggaag caaattctat acttcctaag aaaactcctt tgagcagtat gtctcctaca attgtattta aaaataaaga acctttttta gttactggtg ctcctggagg ttcttatatc gtaaacgccg ttttacagtc tttgatctat aatttagatt ttaatctgac tttgtatgaa tccgtagcca ggggaagaat acatcatcaa ttttttccgg atgcggtatt tatagaaaaa tcagtaaatg aaagaaacgt atttgatggt ctttcctcta aaaaacacga tcttagaatt gctccgaact ttgcgaaact attttctgtc aaaagagaga atggaatgct ctatggagct tgcgatccac gtggagaagg ggccacaggc ggactttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LpinA.18064.a.B2.PW38638

PURIFICATION DATe:	6/18/2019
CONCENTRATION:	11.5mg/ml
OBSERVED MW:	61kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1 mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	55µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAAAAAAA TTTCCACCGA TCTGCTCGTA GTTCCTTCCC ACCAAAAAAC GCATACTGAT TACTTTAGTG AAAGTAAAAA TTTAATGATA TCCTCCGATT CTCCTGAGGC GACACAGGCC GGGATCGAAG TAGGCGCGTT AGGCGGAAAT GTAGTGGACG TAGTAGTAGC GACTTCTTTT GCAATTTCTG TTACAAGACC CCATTCTACT GGTTTGGGAG GAGGAGGTTT TTTAATTCTT TATTTAAAAG AATTTCCAGA ACCGATCGCT TTCGATTTTA GAGAAAGGGC GCCGAATACG TCTTCTAGAG ATATGTATAA ACTAAAACCT AAGGAAGATT CTCTTTTAGG GTTTAGGGCA GTAGGTGTTC CGGGTAACGT TGCAGGGCTT GTGCAGATCC AAAGACGTTT TGGAAAACTT CCGCTTAAAA CTGTGATCTC CCCCTCGATC CGACTCGCCG AGAATGGATT TCCAGTTTAT CCAGACCTTC AGTCCGCAAT TCAAAAATCT TCTAAAGATA TGAGCGAAGA AATGAAAAAT ATCTTTCTTC CCAAAGGAAA AATTCCGGAG TTAAACAGTA TTCTCGTTCA AAAGGATCTT GCAAATTCTC TCAGACTCAT TTCCGAAACT GGCGATAAAG AGTTTTATCA CGGTAAAATT GCGAATTCGA TTGTCAATGC GATGAAAAAA AACGATGGTC TTATCACTCT ACAAGATCTG AGCGGTTATA AAGTAATCGA GAAAAAAACA GTACATACAA CTTACCACGA TTATACGATT TACACGATGC CTCCTCCTTC TTCTGGAGTT CATTTACTGA CTATGTTGTC TATGGTGGAA ACAAAACCTC TGAAAGAGAC GTATGAAAAA GATCCTGTTT TATATTATCA TTTTATTGTA GAAGCGATGA GAAGAGGTTA TGCGGACAGA GCGATGTTAG GTGGAGATCC TGCCTTTACA AAAATTCCAA TTGAACGTCT TGTTTCTAAA AAATACGCGG AAGAAAAAAT TTCAAATTTT AATCCTAAAA CAGCTTCTAG TAGCTCTTCT TTTTTAAAAA CTTTAAACTT TGGAGTAGAA TCTCCACAAA CTACACATAT TTCCGTTATG GATCGTGAAG GAAATTCAGT TTCTACAACT CAGTCTATCA ATTTTAGATT TGGAGCTTCT GTAGTCGCTC CTGGAACTGG AATTGTGTTA AACGATACAA TGGATGATTT TAGTCGAGCT CCAGGAGAAC CAAATGTATA CGGTTTAATC GGTGCGGAAG CAAATTCTAT ACTTCCTAAG AAAACTCCTT TGAGCAGTAT GTCTCCTACA ATTGTATTTA AAAATAAAGA ACCTTTTTTA GTTACTGGTG CTCCTGGAGG TTCTTATATC GTAAACGCCG TTTTACAGTC TTTGATCTAT AATTTAGATT TTAATCTGAC TTTGTATGAA TCCGTAGCCA GGGGAAGAAT ACATCATCAA TTTTTTCCGG ATGCGGTATT TATAGAAAAA TCAGTAAATG AAAGAAACGT ATTTGATGGT CTTTCCTCTA AAAAACACGA TCTTAGAATT GCTCCGAACT TTGCGAAACT ATTTTCTGTC AAAAGAGAGA ATGGAATGCT CTATGGAGCT TGCGATCCAC GTGGAGAAGG GGCCACAGGC GGACTTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T