SSGCID: Target Page

LpinA.17844.a

Ankyrin-like protein

CENTER ID:	LpinA.17844.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.17844.a.B2.GE42894	mature protein		20	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.3635
UniProt:	Q72N28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKILFRMMIT ILMAAQMDCA TIQMLEDAQF LPTPVWSMAR YADKTISPEL SKNPNVWNPP LWIYPGTRRY WMTGINKNFP FSVIDLTLSF LADTILLPIT IPLTAYYYWR VPEAWESGLL IGSIENNDLS GVRRCIETGA DVNSTFIGPP PLYFAIKNEN VEIVRLLLEK GADVNFQNNT FYDQSLLYFA IEKENTEIVE LLLKKGADAN AKSCDLFRRC NYLFFKVIER YSNLSPKKDK RPEIVRLLLE KGGADVNATL AEDGTSPLHY AVEKKHNIQI VKQLVEHGAN VNGKDVYQQT PLYDVASKEI AQYLLQQGAD PTVKNSTGET PLERAKYLMN LDRKNNYDAL KFLEDTP

NT Sequence

atgaaaatct tgtttagaat gatgataacg atcttgatgg ccgctcagat ggattgcgcc acaattcaaa tgcttgaaga cgcccaattt ttacccacac ccgtttggtc catggcaaga tacgcagata aaaccatctc tccggaactg tctaaaaatc ctaacgtctg gaatcctccc ctctggattt atccagggac acgacgctac tggatgactg gtatcaataa gaattttcct tttagtgtta ttgatcttac tttgtccttc ttggcggata cgatcctact tcctattaca attccgttga cggcatacta ctattggaga gttcccgagg cctgggagtc aggccttctg atcggaagta tcgagaacaa cgatctatcc ggagttcgcc gatgcatcga gactggcgcg gatgtaaatt ccacattcat tggtccacct ccattgtatt ttgctattaa gaacgagaac gttgaaattg tgagactcct cttagagaaa ggcgccgatg tgaatttcca aaacaacacc ttctatgatc aatctttatt gtattttgct attgagaaag agaacactga aatcgttgag cttcttttaa aaaaaggcgc cgatgcaaat gcaaagagtt gcgatctttt tcgccgttgt aattatctat tcttcaaagt aatagaacgc tattctaatc tttccccaaa aaaagataaa agacccgaaa ttgtcagact gctcttagaa aagggaggag ccgatgtcaa tgctactttg gcggaagacg gaacaagtcc tctacactac gcagtggaga aaaaacataa tattcaaatt gtaaaacaat tagtagaaca cggcgccaac gtaaacggta aagatgttta tcagcagacc ccactgtatg atgtagcctc aaaagaaatc gcacaatatc ttttgcaaca aggcgcagat ccaaccgtaa aaaacagcac tggggaaacc cctttggaac gtgcaaaata tctaatgaac ttagacagaa aaaataatta tgatgcactt aagtttcttg aagacactcc ctaa

Details for LpinA.17844.a.B2.GE42894

HARVESTED ON:	2/7/2019
SEQUENCED ON:	2/8/2019
EXPECTED MW:	39kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHAT IQMLEDAQFL PTPVWSMARY ADKTISPELS KNPNVWNPPL WIYPGTRRYW MTGINKNFPF SVIDLTLSFL ADTILLPITI PLTAYYYWRV PEAWESGLLI GSIENNDLSG VRRCIETGAD VNSTFIGPPP LYFAIKNENV EIVRLLLEKG ADVNFQNNTF YDQSLLYFAI EKENTEIVEL LLKKGADANA KSCDLFRRCN YLFFKVIERY SNLSPKKDKR PEIVRLLLEK GGADVNATLA EDGTSPLHYA VEKKHNIQIV KQLVEHGANV NGKDVYQQTP LYDVASKEIA QYLLQQGADP TVKNSTGETP LERAKYLMNL DRKNNYDALK FLEDTP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccacaa ttcaaatgct tgaagacgcc caatttttac ccacacccgt ttggtccatg gcaagatacg cagataaaac catctctccg gaactgtcta aaaatcctaa cgtctggaat cctcccctct ggatttatcc agggacacga cgctactgga tgactggtat caataagaat tttcctttta gtgttattga tcttactttg tccttcttgg cggatacgat cctacttcct attacaattc cgttgacggc atactactat tggagagttc ccgaggcctg ggagtcaggc cttctgatcg gaagtatcga gaacaacgat ctatccggag ttcgccgatg catcgagact ggcgcggatg taaattccac attcattggt ccacctccat tgtattttgc tattaagaac gagaacgttg aaattgtgag actcctctta gagaaaggcg ccgatgtgaa tttccaaaac aacaccttct atgatcaatc tttattgtat tttgctattg agaaagagaa cactgaaatc gttgagcttc ttttaaaaaa aggcgccgat gcaaatgcaa agagttgcga tctttttcgc cgttgtaatt atctattctt caaagtaata gaacgctatt ctaatctttc cccaaaaaaa gataaaagac ccgaaattgt cagactgctc ttagaaaagg gaggagccga tgtcaatgct actttggcgg aagacggaac aagtcctcta cactacgcag tggagaaaaa acataatatt caaattgtaa aacaattagt agaacacggc gccaacgtaa acggtaaaga tgtttatcag cagaccccac tgtatgatgt agcctcaaaa gaaatcgcac aatatctttt gcaacaaggc gcagatccaa ccgtaaaaaa cagcactggg gaaacccctt tggaacgtgc aaaatatcta atgaacttag acagaaaaaa taattatgat gcacttaagt ttcttgaaga cactccctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at