LpinA.17356.a

OppA

CENTER ID: LpinA.17356.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.17356.a.B2.GE41954 mature protein 22 599
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.17356.a.B2.PS38549 mature protein 22 599
Molecular models
METHOD RESULTS
comparative modelling Robetta_2675
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|267671.5.peg.1252
UniProt: Q72TK6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKILYSVFC ILFFCLLPVN CGDKEEVESV VVETLPWKGN PDSIPLALRT QNPIVSPDAK KGGTFRIYSN QFPKSLNYYL DQFSTTAHIF GLMFEPLLDY HPITLDPIPH LASSWKISPD KKKFTFKIDE NAFWSDGKPV TANDVLFTYE TLMDKNNNTA VFRIDLSRFE KPVVLNEREI EFTQKEIHWS NFNTVANSLY ILPAHYYKDR NFDKENFDFP IVSGPYSMLS AKKGRYVKMR RRGDYWMRAY PFYKGTDNFD TILFKVYNEE PIAFQAFKKG DIDIYPTYTA SFWVKEAVGE KFDENYILKQ KIYNSKPIGF QGLVFNIRRG IFSDVRVRKA FAHLVDRKLL VEKLAYNEYE LTNSFYQDLW PTNFSPNPPI DFDVKKAREL LSEAGWKTNS KGILEKDGKE FRFTILERDK KSEKYLTPIQ ERAKEVGIII NLESTDLAEW SSRMDKYDFD MTWAAWGGGV FKDPEAMWYS KYADEKGQPN LSGFKNSEVD KLIEQQRAEF SVPKRNEILK KIDKILTAEV PYVLLWNTSA TRIMYWNRFQ FPKNPLGKYG SEKEASSLWW LDEERSSKLN DSILKKEKLP SVPDKVYFQ
NT Sequence
ttgaagaaaa tattatattc tgttttttgt attttgtttt tttgtcttct tcccgttaat tgtggggata aagaagaagt cgagtccgtt gtagttgaaa cccttccttg gaagggaaat ccagattcta tccctttggc tctaagaact caaaatccta tcgtttcacc ggacgctaaa aaaggcggga cgtttcgtat ttacagtaat caatttccta aatccttaaa ttattatttg gatcagtttt ctactacggc tcacatattt ggtctgatgt ttgagcctct tttggattat cacccaatta ctttggatcc aattccacac cttgcttctt cttggaaaat ttctccagat aaaaagaaat ttacttttaa gattgatgaa aacgcgtttt ggtcggacgg caaaccggtt actgcgaatg acgttttatt tacctacgaa accttgatgg ataaaaataa taatactgcg gtttttagaa tcgatctttc tcgttttgaa aaaccggttg ttttaaacga aagggaaatt gaatttactc aaaaagaaat tcactggtct aacttcaaca cagtcgcaaa ttctttgtat attcttcctg ctcattatta taaagataga aatttcgata aggagaattt tgattttccg atcgtatcag gtccttattc catgctttct gctaaaaaag gccgttatgt gaagatgaga agaaggggtg attattggat gagagcttat cctttttata aaggaacgga taattttgac accattctat tcaaagttta taatgaagag ccaatcgcct ttcaagcatt caaaaaaggt gatatagata tttatccaac ttatactgct tctttttggg taaaagaagc agtgggcgaa aagtttgacg agaattatat cctcaaacaa aagatttata attccaaacc gatcggtttt caaggtttgg tttttaatat aagaagagga attttttcgg atgttagagt ccgaaaggca tttgctcatt tagtggatcg gaaacttctc gtcgaaaaat tggcttataa cgaatacgaa ctgacaaact cattttatca ggatctatgg cctacaaatt tttctcctaa tcctccgatc gattttgacg taaagaaggc aagagaatta ctttcggaag cgggatggaa aacgaactcg aaaggtattt tagaaaaaga tggaaaagaa tttagattca ctattttaga aagggataag aagtctgaga aatatctgac tcctattcaa gaaagggcta aggaagttgg gattataatc aatttagaat ccacggatct tgcagagtgg agttctagaa tggataaata tgactttgat atgacttggg ctgcttgggg tggaggtgtt tttaaagatc cagaagctat gtggtattct aaatacgcgg atgaaaaagg acaaccgaat ctttctggtt ttaaaaactc agaagtagac aagttgattg aacaacaaag ggctgaattt agtgttccaa agagaaatga gattcttaaa aagatagata agatattaac cgcagaagtt ccatatgtac ttttatggaa tacgagtgcg actagaatta tgtattggaa tcgttttcaa tttcctaaaa atcctcttgg aaaatacgga agtgaaaaag aagcttcttc tctttggtgg ctggatgaag aacgatcttc taaattaaac gactctattt taaaaaagga aaagttacct tcggttccgg ataaagttta ctttcaatag
Details for LpinA.17356.a.B2.GE41954
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 69kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG DKEEVESVVV ETLPWKGNPD SIPLALRTQN PIVSPDAKKG GTFRIYSNQF PKSLNYYLDQ FSTTAHIFGL MFEPLLDYHP ITLDPIPHLA SSWKISPDKK KFTFKIDENA FWSDGKPVTA NDVLFTYETL MDKNNNTAVF RIDLSRFEKP VVLNEREIEF TQKEIHWSNF NTVANSLYIL PAHYYKDRNF DKENFDFPIV SGPYSMLSAK KGRYVKMRRR GDYWMRAYPF YKGTDNFDTI LFKVYNEEPI AFQAFKKGDI DIYPTYTASF WVKEAVGEKF DENYILKQKI YNSKPIGFQG LVFNIRRGIF SDVRVRKAFA HLVDRKLLVE KLAYNEYELT NSFYQDLWPT NFSPNPPIDF DVKKARELLS EAGWKTNSKG ILEKDGKEFR FTILERDKKS EKYLTPIQER AKEVGIIINL ESTDLAEWSS RMDKYDFDMT WAAWGGGVFK DPEAMWYSKY ADEKGQPNLS GFKNSEVDKL IEQQRAEFSV PKRNEILKKI DKILTAEVPY VLLWNTSATR IMYWNRFQFP KNPLGKYGSE KEASSLWWLD EERSSKLNDS ILKKEKLPSV PDKVYFQ
Validated NT Sequence
atggctcacc accaccacca ccatatgggg gataaagaag aagtcgagtc cgttgtagtt gaaacccttc cttggaaggg aaatccagat tctatccctt tggctctaag aactcaaaat cctatcgttt caccggacgc taaaaaaggc gggacgtttc gtatttacag taatcaattt cctaaatcct taaattatta tttggatcag ttttctacta cggctcacat atttggtctg atgtttgagc ctcttttgga ttatcaccca attactttgg atccaattcc acaccttgct tcttcttgga aaatttctcc agataaaaag aaatttactt ttaagattga tgaaaacgcg ttttggtcgg acggcaaacc ggttactgcg aatgacgttt tatttaccta cgaaaccttg atggataaaa ataataatac tgcggttttt agaatcgatc tttctcgttt tgaaaaaccg gttgttttaa acgaaaggga aattgaattt actcaaaaag aaattcactg gtctaacttc aacacagtcg caaattcttt gtatattctt cctgctcatt attataaaga tagaaatttc gataaggaga attttgattt tccgatcgta tcaggtcctt attccatgct ttctgctaaa aaaggccgtt atgtgaagat gagaagaagg ggtgattatt ggatgagagc ttatcctttt tataaaggaa cggataattt tgacaccatt ctattcaaag tttataatga agagccaatc gcctttcaag cattcaaaaa aggtgatata gatatttatc c-aacttata ctgcttcttt ttgggtaaaa -gaagcagtg ggcgaaaagt ttgacgagaa ttatatcctc aaacaaaaga tttataattc caaaccgatc ggttttcaag gtttggtttt taatataaga agaggaattt tttcggatgt tagagtccga aaggcatttg ctcatttagt ggatcggaaa cttctcgtcg aaaaattggc ttataacgaa tacgaactga caaactcatt ttatcaggat ctatggccta caaatttttc tcctaatcct ccgatcgatt ttgacgtaaa gaaggcaaga gaattacttt cggaagcggg atggaaaacg aactcgaaag gtattttaga aaaagatgga aaagaattta gattcactat tttagaaagg gataagaagt ctgagaaata tctgactcct attcaagaaa gggctaagga agttgggatt ataatcaatt tagaatccac ggatcttgca gagtggagtt ctagaatgga taaatatgac tttgatatga cttgggctgc ttggggtgga ggtgttttta aagatccaga agctatgtgg tattctaaat acgcggatga aaaaggacaa ccgaatcttt ctggttttaa aaactcagaa gtagacaagt tgattgaaca acaaagggct gaatttagtg ttccaaagag aaatgagatt cttaaaaaga tagataagat attaaccgca gaagttccat atgtactttt atggaatacg agtgcgacta gaattatgta ttggaatcgt tttcaatttc ctaaaaatcc tcttggaaaa tacggaagtg aaaaagaagc ttcttctctt tggtggctgg atgaagaacg atcttctaaa ttaaacgact ctattttaaa aaaggaaaag ttaccttcgg ttccggataa agtttacttt caataa
Expected Protein Sequence
MAHHHHHHGD KEEVESVVVE TLPWKGNPDS IPLALRTQNP IVSPDAKKGG TFRIYSNQFP KSLNYYLDQF STTAHIFGLM FEPLLDYHPI TLDPIPHLAS SWKISPDKKK FTFKIDENAF WSDGKPVTAN DVLFTYETLM DKNNNTAVFR IDLSRFEKPV VLNEREIEFT QKEIHWSNFN TVANSLYILP AHYYKDRNFD KENFDFPIVS GPYSMLSAKK GRYVKMRRRG DYWMRAYPFY KGTDNFDTIL FKVYNEEPIA FQAFKKGDID IYPTYTASFW VKEAVGEKFD ENYILKQKIY NSKPIGFQGL VFNIRRGIFS DVRVRKAFAH LVDRKLLVEK LAYNEYELTN SFYQDLWPTN FSPNPPIDFD VKKARELLSE AGWKTNSKGI LEKDGKEFRF TILERDKKSE KYLTPIQERA KEVGIIINLE STDLAEWSSR MDKYDFDMTW AAWGGGVFKD PEAMWYSKYA DEKGQPNLSG FKNSEVDKLI EQQRAEFSVP KRNEILKKID KILTAEVPYV LLWNTSATRI MYWNRFQFPK NPLGKYGSEK EASSLWWLDE ERSSKLNDSI LKKEKLPSVP DKVYFQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggggata aagaagaagt cgagtccgtt gtagttgaaa cccttccttg gaagggaaat ccagattcta tccctttggc tctaagaact caaaatccta tcgtttcacc ggacgctaaa aaaggcggga cgtttcgtat ttacagtaat caatttccta aatccttaaa ttattatttg gatcagtttt ctactacggc tcacatattt ggtctgatgt ttgagcctct tttggattat cacccaatta ctttggatcc aattccacac cttgcttctt cttggaaaat ttctccagat aaaaagaaat ttacttttaa gattgatgaa aacgcgtttt ggtcggacgg caaaccggtt actgcgaatg acgttttatt tacctacgaa accttgatgg ataaaaataa taatactgcg gtttttagaa tcgatctttc tcgttttgaa aaaccggttg ttttaaacga aagggaaatt gaatttactc aaaaagaaat tcactggtct aacttcaaca cagtcgcaaa ttctttgtat attcttcctg ctcattatta taaagataga aatttcgata aggagaattt tgattttccg atcgtatcag gtccttattc catgctttct gctaaaaaag gccgttatgt gaagatgaga agaaggggtg attattggat gagagcttat cctttttata aaggaacgga taattttgac accattctat tcaaagttta taatgaagag ccaatcgcct ttcaagcatt caaaaaaggt gatatagata tttatccaac ttatactgct tctttttggg taaaagaagc agtgggcgaa aagtttgacg agaattatat cctcaaacaa aagatttata attccaaacc gatcggtttt caaggtttgg tttttaatat aagaagagga attttttcgg atgttagagt ccgaaaggca tttgctcatt tagtggatcg gaaacttctc gtcgaaaaat tggcttataa cgaatacgaa ctgacaaact cattttatca ggatctatgg cctacaaatt tttctcctaa tcctccgatc gattttgacg taaagaaggc aagagaatta ctttcggaag cgggatggaa aacgaactcg aaaggtattt tagaaaaaga tggaaaagaa tttagattca ctattttaga aagggataag aagtctgaga aatatctgac tcctattcaa gaaagggcta aggaagttgg gattataatc aatttagaat ccacggatct tgcagagtgg agttctagaa tggataaata tgactttgat atgacttggg ctgcttgggg tggaggtgtt tttaaagatc cagaagctat gtggtattct aaatacgcgg atgaaaaagg acaaccgaat ctttctggtt ttaaaaactc agaagtagac aagttgattg aacaacaaag ggctgaattt agtgttccaa agagaaatga gattcttaaa aagatagata agatattaac cgcagaagtt ccatatgtac ttttatggaa tacgagtgcg actagaatta tgtattggaa tcgttttcaa tttcctaaaa atcctcttgg aaaatacgga agtgaaaaag aagcttcttc tctttggtgg ctggatgaag aacgatcttc taaattaaac gactctattt taaaaaagga aaagttacct tcggttccgg ataaagttta ctttcaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LpinA.17356.a.B2.PS38549
PURIFICATION DATe: 1/1/2019
CONCENTRATION: 30mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG DKEEVESVVV ETLPWKGNPD SIPLALRTQN PIVSPDAKKG GTFRIYSNQF PKSLNYYLDQ FSTTAHIFGL MFEPLLDYHP ITLDPIPHLA SSWKISPDKK KFTFKIDENA FWSDGKPVTA NDVLFTYETL MDKNNNTAVF RIDLSRFEKP VVLNEREIEF TQKEIHWSNF NTVANSLYIL PAHYYKDRNF DKENFDFPIV SGPYSMLSAK KGRYVKMRRR GDYWMRAYPF YKGTDNFDTI LFKVYNEEPI AFQAFKKGDI DIYPTYTASF WVKEAVGEKF DENYILKQKI YNSKPIGFQG LVFNIRRGIF SDVRVRKAFA HLVDRKLLVE KLAYNEYELT NSFYQDLWPT NFSPNPPIDF DVKKARELLS EAGWKTNSKG ILEKDGKEFR FTILERDKKS EKYLTPIQER AKEVGIIINL ESTDLAEWSS RMDKYDFDMT WAAWGGGVFK DPEAMWYSKY ADEKGQPNLS GFKNSEVDKL IEQQRAEFSV PKRNEILKKI DKILTAEVPY VLLWNTSATR IMYWNRFQFP KNPLGKYGSE KEASSLWWLD EERSSKLNDS ILKKEKLPSV PDKVYFQ
Validated NT Sequence
atggctcacc accaccacca ccatatgggg gataaagaag aagtcgagtc cgttgtagtt gaaacccttc cttggaaggg aaatccagat tctatccctt tggctctaag aactcaaaat cctatcgttt caccggacgc taaaaaaggc gggacgtttc gtatttacag taatcaattt cctaaatcct taaattatta tttggatcag ttttctacta cggctcacat atttggtctg atgtttgagc ctcttttgga ttatcaccca attactttgg atccaattcc acaccttgct tcttcttgga aaatttctcc agataaaaag aaatttactt ttaagattga tgaaaacgcg ttttggtcgg acggcaaacc ggttactgcg aatgacgttt tatttaccta cgaaaccttg atggataaaa ataataatac tgcggttttt agaatcgatc tttctcgttt tgaaaaaccg gttgttttaa acgaaaggga aattgaattt actcaaaaag aaattcactg gtctaacttc aacacagtcg caaattcttt gtatattctt cctgctcatt attataaaga tagaaatttc gataaggaga attttgattt tccgatcgta tcaggtcctt attccatgct ttctgctaaa aaaggccgtt atgtgaagat gagaagaagg ggtgattatt ggatgagagc ttatcctttt tataaaggaa cggataattt tgacaccatt ctattcaaag tttataatga agagccaatc gcctttcaag cattcaaaaa aggtgatata gatatttatc c-aacttata ctgcttcttt ttgggtaaaa -gaagcagtg ggcgaaaagt ttgacgagaa ttatatcctc aaacaaaaga tttataattc caaaccgatc ggttttcaag gtttggtttt taatataaga agaggaattt tttcggatgt tagagtccga aaggcatttg ctcatttagt ggatcggaaa cttctcgtcg aaaaattggc ttataacgaa tacgaactga caaactcatt ttatcaggat ctatggccta caaatttttc tcctaatcct ccgatcgatt ttgacgtaaa gaaggcaaga gaattacttt cggaagcggg atggaaaacg aactcgaaag gtattttaga aaaagatgga aaagaattta gattcactat tttagaaagg gataagaagt ctgagaaata tctgactcct attcaagaaa gggctaagga agttgggatt ataatcaatt tagaatccac ggatcttgca gagtggagtt ctagaatgga taaatatgac tttgatatga cttgggctgc ttggggtgga ggtgttttta aagatccaga agctatgtgg tattctaaat acgcggatga aaaaggacaa ccgaatcttt ctggttttaa aaactcagaa gtagacaagt tgattgaaca acaaagggct gaatttagtg ttccaaagag aaatgagatt cttaaaaaga tagataagat attaaccgca gaagttccat atgtactttt atggaatacg agtgcgacta gaattatgta ttggaatcgt tttcaatttc ctaaaaatcc tcttggaaaa tacggaagtg aaaaagaagc ttcttctctt tggtggctgg atgaagaacg atcttctaaa ttaaacgact ctattttaaa aaaggaaaag ttaccttcgg ttccggataa agtttacttt caataa
Expressed Protein Sequence
MAHHHHHHGD KEEVESVVVE TLPWKGNPDS IPLALRTQNP IVSPDAKKGG TFRIYSNQFP KSLNYYLDQF STTAHIFGLM FEPLLDYHPI TLDPIPHLAS SWKISPDKKK FTFKIDENAF WSDGKPVTAN DVLFTYETLM DKNNNTAVFR IDLSRFEKPV VLNEREIEFT QKEIHWSNFN TVANSLYILP AHYYKDRNFD KENFDFPIVS GPYSMLSAKK GRYVKMRRRG DYWMRAYPFY KGTDNFDTIL FKVYNEEPIA FQAFKKGDID IYPTYTASFW VKEAVGEKFD ENYILKQKIY NSKPIGFQGL VFNIRRGIFS DVRVRKAFAH LVDRKLLVEK LAYNEYELTN SFYQDLWPTN FSPNPPIDFD VKKARELLSE AGWKTNSKGI LEKDGKEFRF TILERDKKSE KYLTPIQERA KEVGIIINLE STDLAEWSSR MDKYDFDMTW AAWGGGVFKD PEAMWYSKYA DEKGQPNLSG FKNSEVDKLI EQQRAEFSVP KRNEILKKID KILTAEVPYV LLWNTSATRI MYWNRFQFPK NPLGKYGSEK EASSLWWLDE ERSSKLNDSI LKKEKLPSVP DKVYFQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGGGATA AAGAAGAAGT CGAGTCCGTT GTAGTTGAAA CCCTTCCTTG GAAGGGAAAT CCAGATTCTA TCCCTTTGGC TCTAAGAACT CAAAATCCTA TCGTTTCACC GGACGCTAAA AAAGGCGGGA CGTTTCGTAT TTACAGTAAT CAATTTCCTA AATCCTTAAA TTATTATTTG GATCAGTTTT CTACTACGGC TCACATATTT GGTCTGATGT TTGAGCCTCT TTTGGATTAT CACCCAATTA CTTTGGATCC AATTCCACAC CTTGCTTCTT CTTGGAAAAT TTCTCCAGAT AAAAAGAAAT TTACTTTTAA GATTGATGAA AACGCGTTTT GGTCGGACGG CAAACCGGTT ACTGCGAATG ACGTTTTATT TACCTACGAA ACCTTGATGG ATAAAAATAA TAATACTGCG GTTTTTAGAA TCGATCTTTC TCGTTTTGAA AAACCGGTTG TTTTAAACGA AAGGGAAATT GAATTTACTC AAAAAGAAAT TCACTGGTCT AACTTCAACA CAGTCGCAAA TTCTTTGTAT ATTCTTCCTG CTCATTATTA TAAAGATAGA AATTTCGATA AGGAGAATTT TGATTTTCCG ATCGTATCAG GTCCTTATTC CATGCTTTCT GCTAAAAAAG GCCGTTATGT GAAGATGAGA AGAAGGGGTG ATTATTGGAT GAGAGCTTAT CCTTTTTATA AAGGAACGGA TAATTTTGAC ACCATTCTAT TCAAAGTTTA TAATGAAGAG CCAATCGCCT TTCAAGCATT CAAAAAAGGT GATATAGATA TTTATCCAAC TTATACTGCT TCTTTTTGGG TAAAAGAAGC AGTGGGCGAA AAGTTTGACG AGAATTATAT CCTCAAACAA AAGATTTATA ATTCCAAACC GATCGGTTTT CAAGGTTTGG TTTTTAATAT AAGAAGAGGA ATTTTTTCGG ATGTTAGAGT CCGAAAGGCA TTTGCTCATT TAGTGGATCG GAAACTTCTC GTCGAAAAAT TGGCTTATAA CGAATACGAA CTGACAAACT CATTTTATCA GGATCTATGG CCTACAAATT TTTCTCCTAA TCCTCCGATC GATTTTGACG TAAAGAAGGC AAGAGAATTA CTTTCGGAAG CGGGATGGAA AACGAACTCG AAAGGTATTT TAGAAAAAGA TGGAAAAGAA TTTAGATTCA CTATTTTAGA AAGGGATAAG AAGTCTGAGA AATATCTGAC TCCTATTCAA GAAAGGGCTA AGGAAGTTGG GATTATAATC AATTTAGAAT CCACGGATCT TGCAGAGTGG AGTTCTAGAA TGGATAAATA TGACTTTGAT ATGACTTGGG CTGCTTGGGG TGGAGGTGTT TTTAAAGATC CAGAAGCTAT GTGGTATTCT AAATACGCGG ATGAAAAAGG ACAACCGAAT CTTTCTGGTT TTAAAAACTC AGAAGTAGAC AAGTTGATTG AACAACAAAG GGCTGAATTT AGTGTTCCAA AGAGAAATGA GATTCTTAAA AAGATAGATA AGATATTAAC CGCAGAAGTT CCATATGTAC TTTTATGGAA TACGAGTGCG ACTAGAATTA TGTATTGGAA TCGTTTTCAA TTTCCTAAAA ATCCTCTTGG AAAATACGGA AGTGAAAAAG AAGCTTCTTC TCTTTGGTGG CTGGATGAAG AACGATCTTC TAAATTAAAC GACTCTATTT TAAAAAAGGA AAAGTTACCT TCGGTTCCGG ATAAAGTTTA CTTTCAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT