SSGCID: Target Page

LpinA.01671.a

Histidine kinase sensor protein

CENTER ID:	LpinA.01671.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.01671.a.B1.GE41894	full length		1	739

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.2419
UniProt:	Q72QS8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIHILKAILE QAHAGYWEKN FSENKSYLSP GWKSMLGYQK EELEDSISAW QSHIFPEDLN PIRNEFEDYL KTDLSQSYEA DIRFLHKNGN IIWFKCTCKV VEKDSLGKPI LMIGTYFDVT KSKKIESELQ HTVDRLALAT QTARVGIWDL DFTSGKISWD DTMFELYGIT REKFSGTVLD WESSLHPDDL ERCRSEYAEA LSGEKEFDTQ FRVVWPDGSI HFIKAIAIVQ RMASGKPFRM LGTNWDITKH KNAEIALKES YELTKVFIEN APSAIAMFDT NMKYMAASQQ WLVDYYLGGI EIIGRSHYEI FPEIGDEWKS IHQECLRGRV IRKEEEAFTR LDGTVQWIIW EVRPWYASPN VVGGILMYTA DITTLKRKEF ERSKLEEILT RTNEAAQIGS WELDLETNTR VWSKVTKAIF ELPEDYKPDG KEVGNFFRNE EDRKKSADIL TESIQTGKPF DVEVEILTAK GNVVWTRSIG KPEYKDGKCI RVSGTFQNIQ EQKLKELALQ RTLDLLNNQN ERLLNFAHIV SHNLRSHSGN ISMLLKILQE SKNESEKEKM IQYIAKASDS LMDTVWNLNE VVSIQTNKNL QNAEISLRDY IEKATQTIGG EIQKYQVQIH NLVSDSIRVR CNASYMESIL LNFLTNAVKY RHPDRVPEIT LNAERQENRL ILSIKDNGLG IDMKKYGDKL FGMYKTFHNN KDAKGIGLFI TKNQVEAMGG KIEVESRLNN GTTFRIIFL

NT Sequence

atgattcaca ttcttaaagc tattttagaa caggcacacg caggatattg ggaaaaaaat ttttctgaaa ataaaagtta tttgagccct ggttggaaat ctatgttggg ctatcaaaaa gaagaattgg aggactctat atcagcttgg cagtctcata tttttccgga agatctaaat ccaattcgta acgaatttga agattattta aagacggacc tgagtcaatc atacgaagcg gatattcgtt ttttacataa aaacggaaac atcatttggt ttaaatgtac ttgtaaggta gtagaaaaag attcattagg aaaaccgatt ctaatgattg gaacttactt tgacgttaca aaatccaaaa aaatagaatc cgaattacaa cacaccgttg accgtcttgc tcttgccact caaacggcga gggtaggaat ttgggatctg gattttactt ccgggaaaat atcttgggat gatactatgt tcgaacttta tggaattact cgagagaaat tttcaggaac cgtattagac tgggagtcta gtttacatcc ggatgatttg gaaagatgca gatcggaata tgcagaagct ctttctggag aaaaagaatt cgacactcag tttagggtag tctggccgga tggttccata cattttatta aggcgattgc aatcgttcaa agaatggctt ctggaaaacc ttttcggatg ttaggaacga attgggatat taccaaacat aaaaacgcag aaattgcatt aaaagaaagt tatgaactga ctaaagtatt tattgaaaac gctccttctg cgattgctat gtttgatacg aacatgaaat atatggccgc ttcccaacag tggcttgttg attattattt gggcggaata gaaattatcg gtcgttctca ttatgaaatt tttcctgaaa ttggagacga atggaaatcg attcaccaag aatgtttacg tggaagggta attcgaaaag aagaggaagc ttttacccgt ttagatggaa cggttcagtg gattatctgg gaagtaagac cttggtatgc ttctcccaat gtagtaggcg gtattttgat gtataccgca gatattacta ctctgaaacg aaaggagttc gaaagaagta agttggagga aattctaacc aggaccaacg aagccgcgca gataggatct tgggaattgg atttagaaac gaacacaaga gtttggagta aggtgacaaa agcgattttc gaattgccag aagattataa accggatggt aaggaagtag gaaatttttt taggaatgaa gaagatagaa aaaagtctgc ggatattttg acggaatcga ttcaaacggg caaaccgttt gacgtcgaag tggaaatttt gactgcaaag ggaaatgtag tatggactcg atcaattgga aagccggagt ataaagatgg aaagtgtatt cgtgtttcag gtacatttca gaatatccag gaacaaaaat taaaagaact ggctcttcag agaactttag atctattaaa taatcagaat gaaagattat taaattttgc tcatatagtt tcgcataatc tccgttccca ttctggaaat atatcaatgc ttcttaaaat tttacaagaa tctaaaaacg aatctgaaaa agagaagatg atacaatata tcgcaaaggc ttcggacagt ctgatggata cggtttggaa tttgaacgaa gtagtttcaa ttcaaacgaa taaaaatctt caaaatgctg aaattagtct tagagattat atcgaaaagg cgactcagac gatcggagga gaaattcaga aatatcaggt tcaaattcat aatttagttt cggattcaat tcgagttcgt tgtaacgcat cttatatgga aagtattctt ctaaattttc ttacaaatgc tgtaaaatat agacatccgg atagagttcc agagatcact ttaaacgcgg aacgtcaaga gaatcggttg attcttagta ttaaagacaa tggtcttggg atcgatatga aaaaatacgg agataaactt ttcgggatgt ataaaacttt tcacaataat aaggacgcta agggaatcgg attatttatc actaaaaatc aagtagaagc catgggtggg aaaattgaag tggaaagtcg gttgaataat ggaactactt ttagaatcat ttttctttga

Details for LpinA.01671.a.B1.GE41894

HARVESTED ON:	8/31/2017
SEQUENCED ON:	9/29/2017
EXPECTED MW:	86kDa
OBSERVED MW:	86kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMI HILKAILEQA HAGYWEKNFS ENKSYLSPGW KSMLGYQKEE LEDSISAWQS HIFPEDLNPI RNEFEDYLKT DLSQSYEADI RFLHKNGNII WFKCTCKVVE KDSLGKPILM IGTYFDVTKS KKIESELQHT VDRLALATQT ARVGIWDLDF TSGKISWDDT MFELYGITRE KFSGTVLDWE SSLHPDDLER CRSEYAEALS GEKEFDTQFR VVWPDGSIHF IKAIAIVQRM ASGKPFRMLG TNWDITKHKN AEIALKESYE LTKVFIENAP SAIAMFDTNM KYMAASQQWL VDYYLGGIEI IGRSHYEIFP EIGDEWKSIH QECLRGRVIR KEEEAFTRLD GTVQWIIWEV RPWYASPNVV GGILMYTADI TTLKRKEFER SKLEEILTRT NEAAQIGSWE LDLETNTRVW SKVTKAIFEL PEDYKPDGKE VGNFFRNEED RKKSADILTE SIQTGKPFDV EVEILTAKGN VVWTRSIGKP EYKDGKCIRV SGTFQNIQEQ KLKELALQRT LDLLNNQNER LLNFAHIVSH NLRSHSGNIS MLLKILQESK NESEKEKMIQ YIAKASDSLM DTVWNLNEVV SIQTNKNLQN AEISLRDYIE KATQTIGGEI QKYQVQIHNL VSDSIRVRCN ASYMESILLN FLTNAVKYRH PDRVPEITLN AERQENRLIL SIKDNGLGID MKKYGDKLFG MYKTFHNNKD AKGIGLFITK NQVEAMGGKI EVESRLNNGT TFRIIFL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc acattcttaa agctatttta gaacaggcac acgcaggata ttgggaaaaa aatttttctg aaaataaaag ttatttgagc cctggttgga aatctatgtt gggctatcaa aaagaagaat tggaggactc tatatcagct tggcagtctc atatttttcc ggaagatcta aatccaattc gtaacgaatt tgaagattat ttaaagacgg acctgagtca atcatacgaa gcggatattc gttttttaca taaaaacgga aacatcattt ggtttaaatg tacttgtaag gtagtagaaa aagattcatt aggaaaaccg attctaatga ttggaactta ctttgacgtt acaaaatcca aaaaaataga atccgaatta caacacaccg ttgaccgtct tgctcttgcc actcaaacgg cgagggtagg aatttgggat ctggatttta cttccgggaa aatatcttgg gatgatacta tgttcgaact ttatggaatt actcgagaga aattttcagg aaccgtatta gactgggagt ctagtttaca tccggatgat ttggaaagat gcagatcgga atatgcagaa gctctttctg gagaaaaaga attcgacact cagtttaggg tagtctggcc ggatggttcc atacatttta ttaaggcgat tgcaatcgtt caaagaatgg cttctggaaa accttttcgg atgttaggaa cgaattggga tattaccaaa cataaaaacg cagaaattgc attaaaagaa agttatgaac tgactaaagt atttattgaa aacgctcctt ctgcgattgc tatgtttgat acgaacatga aatatatggc cgcttcccaa cagtggcttg ttgattatta tttgggcgga atagaaatta tcggtcgttc tcattatgaa atttttcctg aaattggaga cgaatggaaa tcgattcacc aagaatgttt acgtggaagg gtaattcgaa aagaagagga agcttttacc cgtttagatg gaacggttca gtggattatc tgggaagtaa gaccttggta tgcttctccc aatgtagtag gcggtatttt gatgtatacc gcagatatta ctactctgaa acgaaaggag ttcgaaagaa gtaagttgga ggaaattcta accaggacca acgaagccgc gcagatagga tcttgggaat tggatttaga aacgaacaca agagtttgga gtaaggtgac aaaagcgatt ttcgaattgc cagaagatta taaaccggat ggtaaggaag taggaaattt ttttaggaat gaagaagata gaaaaaagtc tgcggatatt ttgacggaat cgattcaaac gggcaaaccg tttgacgtcg aagtggaaat tttgactgca aagggaaatg tagtatggac tcgatcaatt ggaaagccgg agtataaaga tggaaagtgt attcgtgttt caggtacatt tcagaatatc caggaacaaa aattaaaaga actggctctt cagagaactt tagatctatt aaataatcag aatgaaagat tattaaattt tgctcatata gtttcgcata atctccgttc ccattctgga aatatatcaa tgcttcttaa aattttacaa gaatctaaaa acgaatctga aaaagagaag atgatacaat atatcgcaaa ggcttcggac agtctgatgg atacggtttg gaatttgaac gaagtagttt caattcaaac gaataaaaat cttcaaaatg ctgaaattag tcttagagat tatatcgaaa aggcgactca gacgatcgga ggagaaattc agaaatatca ggttcaaatt cataatttag tttcggattc aattcgagtt cgttgtaacg catcttatat ggaaagtatt cttctaaatt ttcttacaaa tgctgtaaaa tatagacatc cggatagagt tccagagatc actttaaacg cggaacgtca agagaatcgg ttgattctta gtattaaaga caatggtctt gggatcgata tgaaaaaata cggagataaa cttttcggga tgtataaaac ttttcacaat aataaggacg ctaagggaat cggattattt atcactaaaa atcaagtaga agccatgggt gggaaaattg aagtggaaag tcggttgaat aatggaacta cttttagaat catttttctt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat