LpinA.01356.a

Udp-glucose dehydrogenase

CENTER ID: LpinA.01356.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.01356.a.B2.GE41953 mature protein 21 436
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.2732
UniProt: Q72Q20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVCVIGSGY VGLVAGACFA EYGNHVICVD KDETKIANLK KGIIPIYEPG LSELVLNNWK EKRLEFTVSL KEGVEKSDII FIAVGTPTLP DGSSDLSAVF AVAKEIGKSI NGYKVIVDKS TVPVGTAAKV KTIIANETKE EFDVVSNPEF LKEGAAIEDF MRPERVVIGS ETQKAGDLIA QLYAPFVLNG NPILRMGVVS AELTKYACNA FLATKISFAN EIANLCEAVG GNYEDVRKGM GTDSRIGRQF LYAGIGYGGS CFPKDVRALI KTSEDEGAPL QIIRKVEEVN ESQKLRLYEK IIKFYGESGL SGKTFAVWGL SFKPGTDDMR EAPSIPLLLK LHDKNVKLRV YDPVSKETSS IYFEGKVEYS VDAYSALKGA DALLLLTEWR EFREPDFLKI KGLLKNKVIF DGRNQYSPDL MKKEGFQYFS IGKPNV
NT Sequence
atgaaagttt gtgtgattgg aagcggttat gtaggtcttg tcgcgggtgc ttgttttgcg gaatatggaa atcatgtaat ttgcgtagat aaggatgaaa ctaaaattgc aaatcttaaa aaaggaatca ttcctattta tgagccgggg ctttctgaac tcgttttgaa caactggaaa gaaaaaagat tagagtttac agtttctctc aaagaagggg tggaaaagtc ggatattatt tttattgctg tgggaactcc cactctaccg gacggttctt ctgatctttc tgctgttttt gcagtagcaa aggaaatcgg taagtccatc aacggataca aggtaatcgt tgataagtct acagttccgg tcggaactgc ggccaaagtt aaaaccatca tcgcaaacga aactaaagaa gaattcgacg tggtttccaa tccagaattt ttaaaggaag gcgcagcgat tgaagacttt atgcgtcccg aaagagtagt gatcggttct gaaactcaaa aagcagggga tttaattgca caactgtatg ctccttttgt tctaaacgga aatcctattt tgagaatggg agtggtttca gccgagctta cgaaatacgc ctgtaacgcg tttcttgcga ctaaaatttc ttttgcaaat gaaatcgcta atctttgtga agccgtaggt ggaaattacg aagacgttcg taagggaatg ggaaccgatt ccagaatcgg taggcagttt ttatacgcag gaattggata cggaggttcc tgttttccca aggacgttcg agctttgatc aaaacttctg aagatgaagg agctcctctt cagatcattc gtaaagtgga agaggtcaat gaatctcaaa aacttagact ctatgagaaa attataaagt tctatggaga atctggtctt tctggaaaga cgtttgcggt ttgggggctt tcttttaaac caggaacgga cgacatgagg gaggctcctt caattccttt acttttaaaa ttacatgata aaaatgtaaa gttacgggtt tatgatccgg tttctaaaga aacgtcttct atttattttg aagggaaagt agaatattct gtggacgcct attctgcttt gaaaggtgcg gatgctcttt tacttttaac cgagtggaga gaatttagag agcccgattt tttaaagatc aaagggctat taaaaaataa ggttatcttt gacggtagaa atcaatattc tcctgatctg atgaaaaaag aaggctttca atatttctct attggtaaac ctaatgtttg a
Details for LpinA.01356.a.B2.GE41953
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME YGNHVICVDK DETKIANLKK GIIPIYEPGL SELVLNNWKE KRLEFTVSLK EGVEKSDIIF IAVGTPTLPD GSSDLSAVFA VAKEIGKSIN GYKVIVDKST VPVGTAAKVK TIIANETKEE FDVVSNPEFL KEGAAIEDFM RPERVVIGSE TQKAGDLIAQ LYAPFVLNGN PILRMGVVSA ELTKYACNAF LATKISFANE IANLCEAVGG NYEDVRKGMG TDSRIGRQFL YAGIGYGGSC FPKDVRALIK TSEDEGAPLQ IIRKVEEVNE SQKLRLYEKI IKFYGESGLS GKTFAVWGLS FKPGTDDMRE APSIPLLLKL HDKNVKLRVY DPVSKETSSI YFEGKVEYSV DAYSALKGAD ALLLLTEWRE FREPDFLKIK GLLKNKVIFD GRNQYSPDLM KKEGFQ
Validated NT Sequence
attgaaagcc ttcttttttc atcagatcag gagaatattg atttctaccg tcaaagataa ccttattttt taatagccct ttgatcttta aaaaatcggg ctctctaaat tctctccact cggttaaaag taaaagagca tccgcacctt tcaaagcaga ataggcgtcc acagaatatt ctactttccc ttcaaaataa atagaagacg tttctttaga aaccggatca taaacccgta actttacatt tttatcatgt aattttaaaa gtaaaggaat tgaaggagcc tccctcatgt cgtccgttcc tggtttaaaa gaaagccccc aaaccgcaaa cgtctttcca gaaagaccag attctccata gaactttata attttctcat agagtctaag tttttgagat tcattgacct cttccacttt acgaatgatc tgaagaggag ctccttcatc ttcagaagtt ttgatcaaag ctcgaacgtc cttgggaaaa caggaacctc cgtatccaat tcctgcgtat aaaaactgcc taccgattct ggaatcggtt cccattccct tacgaacgtc ttcgtaattt ccacctacgg cttcacaaag attagcgatt tcatttgcaa aagaaatttt agtcgcaaga aacgcgttac aggcgtattt cgtaagctcg gctgaaacca ctcccattct caaaatagga tttccgttta gaacaaaagg agcatacagt tgtgcaatta aatcccctgc tttttgagtt tcagaaccga tcactactct ttcgggacgc ataaagtctt caatcgctgc gccttccttt aaaaattctg gattggaaac cacgtcgaat tcttctttag tttcgtttgc gatgatggtt ttaactttgg ccgcagttcc gaccggaact gtagacttat caacgattac cttgtatccg ttgatggact taccgatttc ctttgctact gcaaaaacag cagaaagatc agaagaaccg tccggtagag tgggagttcc cacagcaata aaaataatat ccgacttttc caccccttct ttgagagaaa ctgtaaactc taatcttttt tctttccagt tgttcaaaac gagttcagaa agccccggct cataaatagg aatgattcct tttttaagat ttgcaatttt agtttcatcc ttatctacgc aaattacatg atttccatat tccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHEY GNHVICVDKD ETKIANLKKG IIPIYEPGLS ELVLNNWKEK RLEFTVSLKE GVEKSDIIFI AVGTPTLPDG SSDLSAVFAV AKEIGKSING YKVIVDKSTV PVGTAAKVKT IIANETKEEF DVVSNPEFLK EGAAIEDFMR PERVVIGSET QKAGDLIAQL YAPFVLNGNP ILRMGVVSAE LTKYACNAFL ATKISFANEI ANLCEAVGGN YEDVRKGMGT DSRIGRQFLY AGIGYGGSCF PKDVRALIKT SEDEGAPLQI IRKVEEVNES QKLRLYEKII KFYGESGLSG KTFAVWGLSF KPGTDDMREA PSIPLLLKLH DKNVKLRVYD PVSKETSSIY FEGKVEYSVD AYSALKGADA LLLLTEWREF REPDFLKIKG LLKNKVIFDG RNQYSPDLMK KEGFQYFSIG KPNV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaatatg gaaatcatgt aatttgcgta gataaggatg aaactaaaat tgcaaatctt aaaaaaggaa tcattcctat ttatgagccg gggctttctg aactcgtttt gaacaactgg aaagaaaaaa gattagagtt tacagtttct ctcaaagaag gggtggaaaa gtcggatatt atttttattg ctgtgggaac tcccactcta ccggacggtt cttctgatct ttctgctgtt tttgcagtag caaaggaaat cggtaagtcc atcaacggat acaaggtaat cgttgataag tctacagttc cggtcggaac tgcggccaaa gttaaaacca tcatcgcaaa cgaaactaaa gaagaattcg acgtggtttc caatccagaa tttttaaagg aaggcgcagc gattgaagac tttatgcgtc ccgaaagagt agtgatcggt tctgaaactc aaaaagcagg ggatttaatt gcacaactgt atgctccttt tgttctaaac ggaaatccta ttttgagaat gggagtggtt tcagccgagc ttacgaaata cgcctgtaac gcgtttcttg cgactaaaat ttcttttgca aatgaaatcg ctaatctttg tgaagccgta ggtggaaatt acgaagacgt tcgtaaggga atgggaaccg attccagaat cggtaggcag tttttatacg caggaattgg atacggaggt tcctgttttc ccaaggacgt tcgagctttg atcaaaactt ctgaagatga aggagctcct cttcagatca ttcgtaaagt ggaagaggtc aatgaatctc aaaaacttag actctatgag aaaattataa agttctatgg agaatctggt ctttctggaa agacgtttgc ggtttggggg ctttctttta aaccaggaac ggacgacatg agggaggctc cttcaattcc tttactttta aaattacatg ataaaaatgt aaagttacgg gtttatgatc cggtttctaa agaaacgtct tctatttatt ttgaagggaa agtagaatat tctgtggacg cctattctgc tttgaaaggt gcggatgctc ttttactttt aaccgagtgg agagaattta gagagcccga ttttttaaag atcaaagggc tattaaaaaa taaggttatc tttgacggta gaaatcaata ttctcctgat ctgatgaaaa aagaaggctt tcaatatttc tctattggta aacctaatgt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat