LpinA.01281.a

GTPase Obg (EC 3.6.5.-) (GTP-binding protein Obg)

CENTER ID: LpinA.01281.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.01281.a.B1.GE41893 full length 1 356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.01281.a.B1.PS38574 full length 1 356

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.3339
UniProt: Q72NQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESFVDEVAI EVFAGHGGAG SVHFRREKYV EFGGPDGGDG GIGGNVVIRP NLSMYTLDKY LSKRKFKAQA GFPGVGDNCS GKKGEDLVLF VPLGTQIYDE ETGDLLFDFV SDSQEFVVAR GGRGGKGNAH FKTSTNQTPR FAQPGEEGEY KFLRLSLKLL ADVGIVGLPN AGKSTLISKI TDAHPKIAGY AFTTLSPNLG VVKRRGDIFR FTIADIPGII EGASMGIGLG LSFLRHIERV KGILYLFDAS SLDIEEDLKM LRNELSTYNP ELLNRPYLIV LNKIDIWNDP EFTKDVIAKV SHLGKVVAIS ADQEVNLEEL LENMDEVFFK NEIEKILNPI KDTKPISLDE SDIFES
NT Sequence
atggaatcct ttgtagatga agtcgccata gaagtattcg ccggtcatgg cggagctggg tccgttcatt ttcgcagaga aaaatacgtc gagtttggag gtccggacgg aggagacgga ggaatcgggg ggaatgtagt cattcgtccc aatctttcta tgtataccct tgataaatat ctatctaaaa ggaaatttaa agctcaggca ggatttccag gagtaggaga taactgttcc ggtaaaaaag gagaagactt agttttgttt gttcctctcg gaacacaaat ctatgacgag gaaacggggg atctgctatt cgattttgta tctgattccc aagaatttgt agtagcccgt ggaggtagag gaggaaaggg aaacgcacat tttaaaactt ctaccaatca aaccccacgt tttgcacaac cgggcgaaga aggagaatat aaattcctac gtctttctct taagttgtta gcggacgtag gaattgtagg tttgccaaac gcaggtaaat ctactttaat ttctaaaatt acagacgcac atccaaaaat agcaggctac gcatttacaa cactttctcc taacctaggc gttgttaaac gaagaggaga tattttccgt tttacgattg ctgatattcc cgggattatc gagggcgcga gtatggggat cggacttgga ctctcctttt tgcgtcatat cgaaagggtc aaaggaatat tatatttgtt tgatgcatct tctcttgata ttgaagagga ccttaaaatg cttcgaaacg aactttccac ttataatcca gaattattaa atcgacccta tctgatcgta ttgaataaga ttgatatttg gaacgatcct gaatttacaa aggatgtgat cgcgaaggta tcacacttag gaaaggtagt tgcgatttcg gcagatcaag aagtaaattt agaagagctt ttagaaaata tggatgaagt ttttttcaaa aatgaaatcg aaaaaattct aaatccaatc aaagatacaa agcctatctc tttggatgaa tcagatattt tcgaaagttg a
Details for LpinA.01281.a.B1.GE41893
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME SFVDEVAIEV FAGHGGAGSV HFRREKYVEF GGPDGGDGGI GGNVVIRPNL SMYTLDKYLS KRKFKAQAGF PGVGDNCSGK KGEDLVLFVP LGTQIYDEET GDLLFDFVSD SQEFVVARGG RGGKGNAHFK TSTNQTPRFA QPGEEGEYKF LRLSLKLLAD VGIVGLPNAG KSTLISKITD AHPKIAGYAF TTLSPNLGVV KRRGDIFRFT IADIPGIIEG ASMGIGLGLS FLRHIERVKG ILYLFDASSL DIEEDLKMLR NELSTYNPEL LNRPYLIVLN KIDIWNDPEF TKDVIAKVSH LGKVVAISAD QEVNLEELLE NMDEVFFKNE IEKILNPIKD TKPIXXDESD IFES
Validated NT Sequence
agataggctt tgtatctttg attggattta gaattttttc gatttcattt ttgaaaaaaa cttcatccat attttctaaa agctcttcta aatttacttc ttgatctgcc gaaatcgcaa ctacctttcc taagtgtgat accttcgcga tcacatcctt tgtaaattca ggatcgttcc aaatatcaat cttattcaat acgatcagat agggtcgatt taataattct ggattataag tggaaagttc gtttcgaagc attttaaggt cctcttcaat atcaagagaa gatgcatcaa acaaatataa tattcctttg accctttcga tatgacgcaa aaaggagagt ccaagtccga tccccatact cgcgccctcg ataatcccgg gaatatcagc aatcgtaaaa cggaaaatat ctcctcttcg tttaacaacg cctaggttag gagaaagtgt tgtaaatgcg tagcctgcta tttttggatg tgcgtctgta attttagaaa ttaaagtaga tttacctgcg tttggcaaac ctacaattcc tacgtccgct aacaacttaa gagaaagacg taggaattta tattctcctt cttcgcccgg ttgtgcaaaa cgtggggttt gattggtaga agttttaaaa tgtgcgtttc cctttcctcc tctacctcca cgggctacta caaattcttg ggaatcagat acaaaatcga atagcagatc ccccgtttcc tcgtcataga tttgtgttcc gagaggaaca aacaaaacta agtcttctcc ttttttaccg gaacagttat ctcctactcc tggaaatcct gcctgagctt taaatttcct tttagataga tatttatcaa gggtatacat agaaagattg ggacgaatga ctacattccc cccgattcct ccgtctcctc cgtccggacc tccaaactcg acgtattttt ctctgcgaaa atgaacggac ccagctccgc catgaccggc gaatacttct atggcgactt catctacaaa ggattccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHME SFVDEVAIEV FAGHGGAGSV HFRREKYVEF GGPDGGDGGI GGNVVIRPNL SMYTLDKYLS KRKFKAQAGF PGVGDNCSGK KGEDLVLFVP LGTQIYDEET GDLLFDFVSD SQEFVVARGG RGGKGNAHFK TSTNQTPRFA QPGEEGEYKF LRLSLKLLAD VGIVGLPNAG KSTLISKITD AHPKIAGYAF TTLSPNLGVV KRRGDIFRFT IADIPGIIEG ASMGIGLGLS FLRHIERVKG ILYLFDASSL DIEEDLKMLR NELSTYNPEL LNRPYLIVLN KIDIWNDPEF TKDVIAKVSH LGKVVAISAD QEVNLEELLE NMDEVFFKNE IEKILNPIKD TKPISLDESD IFES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat cctttgtaga tgaagtcgcc atagaagtat tcgccggtca tggcggagct gggtccgttc attttcgcag agaaaaatac gtcgagtttg gaggtccgga cggaggagac ggaggaatcg gggggaatgt agtcattcgt cccaatcttt ctatgtatac ccttgataaa tatctatcta aaaggaaatt taaagctcag gcaggatttc caggagtagg agataactgt tccggtaaaa aaggagaaga cttagttttg tttgttcctc tcggaacaca aatctatgac gaggaaacgg gggatctgct attcgatttt gtatctgatt cccaagaatt tgtagtagcc cgtggaggta gaggaggaaa gggaaacgca cattttaaaa cttctaccaa tcaaacccca cgttttgcac aaccgggcga agaaggagaa tataaattcc tacgtctttc tcttaagttg ttagcggacg taggaattgt aggtttgcca aacgcaggta aatctacttt aatttctaaa attacagacg cacatccaaa aatagcaggc tacgcattta caacactttc tcctaaccta ggcgttgtta aacgaagagg agatattttc cgttttacga ttgctgatat tcccgggatt atcgagggcg cgagtatggg gatcggactt ggactctcct ttttgcgtca tatcgaaagg gtcaaaggaa tattatattt gtttgatgca tcttctcttg atattgaaga ggaccttaaa atgcttcgaa acgaactttc cacttataat ccagaattat taaatcgacc ctatctgatc gtattgaata agattgatat ttggaacgat cctgaattta caaaggatgt gatcgcgaag gtatcacact taggaaaggt agttgcgatt tcggcagatc aagaagtaaa tttagaagag cttttagaaa atatggatga agtttttttc aaaaatgaaa tcgaaaaaat tctaaatcca atcaaagata caaagcctat ctctttggat gaatcagata ttttcgaaag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for LpinA.01281.a.B1.PS38574
PURIFICATION DATe: 4/16/2019
CONCENTRATION: 14.52mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME SFVDEVAIEV FAGHGGAGSV HFRREKYVEF GGPDGGDGGI GGNVVIRPNL SMYTLDKYLS KRKFKAQAGF PGVGDNCSGK KGEDLVLFVP LGTQIYDEET GDLLFDFVSD SQEFVVARGG RGGKGNAHFK TSTNQTPRFA QPGEEGEYKF LRLSLKLLAD VGIVGLPNAG KSTLISKITD AHPKIAGYAF TTLSPNLGVV KRRGDIFRFT IADIPGIIEG ASMGIGLGLS FLRHIERVKG ILYLFDASSL DIEEDLKMLR NELSTYNPEL LNRPYLIVLN KIDIWNDPEF TKDVIAKVSH LGKVVAISAD QEVNLEELLE NMDEVFFKNE IEKILNPIKD TKPIXXDESD IFES
Validated NT Sequence
agataggctt tgtatctttg attggattta gaattttttc gatttcattt ttgaaaaaaa cttcatccat attttctaaa agctcttcta aatttacttc ttgatctgcc gaaatcgcaa ctacctttcc taagtgtgat accttcgcga tcacatcctt tgtaaattca ggatcgttcc aaatatcaat cttattcaat acgatcagat agggtcgatt taataattct ggattataag tggaaagttc gtttcgaagc attttaaggt cctcttcaat atcaagagaa gatgcatcaa acaaatataa tattcctttg accctttcga tatgacgcaa aaaggagagt ccaagtccga tccccatact cgcgccctcg ataatcccgg gaatatcagc aatcgtaaaa cggaaaatat ctcctcttcg tttaacaacg cctaggttag gagaaagtgt tgtaaatgcg tagcctgcta tttttggatg tgcgtctgta attttagaaa ttaaagtaga tttacctgcg tttggcaaac ctacaattcc tacgtccgct aacaacttaa gagaaagacg taggaattta tattctcctt cttcgcccgg ttgtgcaaaa cgtggggttt gattggtaga agttttaaaa tgtgcgtttc cctttcctcc tctacctcca cgggctacta caaattcttg ggaatcagat acaaaatcga atagcagatc ccccgtttcc tcgtcataga tttgtgttcc gagaggaaca aacaaaacta agtcttctcc ttttttaccg gaacagttat ctcctactcc tggaaatcct gcctgagctt taaatttcct tttagataga tatttatcaa gggtatacat agaaagattg ggacgaatga ctacattccc cccgattcct ccgtctcctc cgtccggacc tccaaactcg acgtattttt ctctgcgaaa atgaacggac ccagctccgc catgaccggc gaatacttct atggcgactt catctacaaa ggattccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHME SFVDEVAIEV FAGHGGAGSV HFRREKYVEF GGPDGGDGGI GGNVVIRPNL SMYTLDKYLS KRKFKAQAGF PGVGDNCSGK KGEDLVLFVP LGTQIYDEET GDLLFDFVSD SQEFVVARGG RGGKGNAHFK TSTNQTPRFA QPGEEGEYKF LRLSLKLLAD VGIVGLPNAG KSTLISKITD AHPKIAGYAF TTLSPNLGVV KRRGDIFRFT IADIPGIIEG ASMGIGLGLS FLRHIERVKG ILYLFDASSL DIEEDLKMLR NELSTYNPEL LNRPYLIVLN KIDIWNDPEF TKDVIAKVSH LGKVVAISAD QEVNLEELLE NMDEVFFKNE IEKILNPIKD TKPISLDESD IFES
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAT CCTTTGTAGA TGAAGTCGCC ATAGAAGTAT TCGCCGGTCA TGGCGGAGCT GGGTCCGTTC ATTTTCGCAG AGAAAAATAC GTCGAGTTTG GAGGTCCGGA CGGAGGAGAC GGAGGAATCG GGGGGAATGT AGTCATTCGT CCCAATCTTT CTATGTATAC CCTTGATAAA TATCTATCTA AAAGGAAATT TAAAGCTCAG GCAGGATTTC CAGGAGTAGG AGATAACTGT TCCGGTAAAA AAGGAGAAGA CTTAGTTTTG TTTGTTCCTC TCGGAACACA AATCTATGAC GAGGAAACGG GGGATCTGCT ATTCGATTTT GTATCTGATT CCCAAGAATT TGTAGTAGCC CGTGGAGGTA GAGGAGGAAA GGGAAACGCA CATTTTAAAA CTTCTACCAA TCAAACCCCA CGTTTTGCAC AACCGGGCGA AGAAGGAGAA TATAAATTCC TACGTCTTTC TCTTAAGTTG TTAGCGGACG TAGGAATTGT AGGTTTGCCA AACGCAGGTA AATCTACTTT AATTTCTAAA ATTACAGACG CACATCCAAA AATAGCAGGC TACGCATTTA CAACACTTTC TCCTAACCTA GGCGTTGTTA AACGAAGAGG AGATATTTTC CGTTTTACGA TTGCTGATAT TCCCGGGATT ATCGAGGGCG CGAGTATGGG GATCGGACTT GGACTCTCCT TTTTGCGTCA TATCGAAAGG GTCAAAGGAA TATTATATTT GTTTGATGCA TCTTCTCTTG ATATTGAAGA GGACCTTAAA ATGCTTCGAA ACGAACTTTC CACTTATAAT CCAGAATTAT TAAATCGACC CTATCTGATC GTATTGAATA AGATTGATAT TTGGAACGAT CCTGAATTTA CAAAGGATGT GATCGCGAAG GTATCACACT TAGGAAAGGT AGTTGCGATT TCGGCAGATC AAGAAGTAAA TTTAGAAGAG CTTTTAGAAA ATATGGATGA AGTTTTTTTC AAAAATGAAA TCGAAAAAAT TCTAAATCCA ATCAAAGATA CAAAGCCTAT CTCTTTGGAT GAATCAGATA TTTTCGAAAG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT