LpinA.00529.a

Sigma 54 activator

CENTER ID: LpinA.00529.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.00529.a.B1.GE41892 full length 1 699
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.143
UniProt: Q72W09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGYVKPSPL RSIQEVATAM NSTLDPDKLL DLILERCIQI CEVGSGSLML INEKENVLDI VTFRGMNPSV RTKVKLKVGE GITGIVAASG EGMIVSDVTA NPHYISIKDD IMSELAVPMI VEDVVIGVIS LDSSRKGAFN EEHLEIISTL ANQAAQIFKN LQIFRQLEQK NKIQQVLIDI SRTVTSTLVL QEIFEDIMDR LEKSLNLERG SIVLFEPEKA ILKLEAASGL TAEEMEKGVY LPGEGVTGKV FETGEPIIIE SIANDENFIN RVGNAAHFKN NPENVSFLAA PIKSDTDVLG VVSVYFVHKK YIDLKTYLDF LQVVASVIYQ AIRIQKLIDE EKREISRENV LLKRELKNKY KFGSLIGKSK PMEKLFEMIH LVSDSRASVL ITGESGTGKE MIASAIHYNS SRADKPFIKI NCAAIPENLL ESELFGHKKG SFTGAVSDKK GKFEMADTGT IFLDEIGEMD LNLQSKLLRV LQEKEIEAVG SVKPKKIDVR VIAATNADLE KLISEKRFRP DLFYRLNVVN MVTPPLRERS DDIPLLINHF IAKYAEENGK KITGVTREAH KLLMNYSWPG NVRELENVIE RAVVLSQLEM LDIQDFSEIN GRLLYGDEDF DPETGDSESS VEIANSRFSS SNLDALDGRA IEVVVGEVEA RLIKYAMKKF KYTKTRVAKF LGINRNTLDK KIKDLKIDY
NT Sequence
atgtcaggat atgtgaagcc tagcccctta agatctatac aagaagttgc tacggcaatg aattctacct tagatccgga taaacttctg gatttgattt tagaacgttg cattcagatt tgcgaagtag gctccggctc tttgatgttg attaatgaga aagaaaacgt tttagatata gtgacttttc gtgggatgaa tccttccgtt cgaactaagg taaaactgaa agttggagaa ggaatcaccg gaattgtagc cgcgtcgggc gaaggtatga tcgtaagtga tgttaccgct aatccacatt acatttccat aaaggatgat attatgtcgg aacttgcggt tccgatgatt gttgaagacg tagtgatcgg agtaatttct ttggactcca gtcgtaaagg agctttcaac gaagaacatt tagaaattat ttctactctt gctaatcaag ctgcccagat ttttaagaac ttacaaattt ttagacaact cgaacaaaaa aataaaattc aacaagtgct aattgatatt tccagaactg ttacttccac tttggttcta caggaaattt ttgaagatat tatggatcgt ttggaaaaat ccctaaattt agaacgcgga agtattgttc ttttcgaacc tgaaaaagcg attttgaaat tggaagcagc ctccggttta actgcggaag aaatggaaaa aggggtttat cttccaggag aaggtgttac tggaaaggtt tttgaaacag gggaaccaat tatcatcgaa tctattgcaa atgacgaaaa ttttataaac cgagttggaa atgcggctca ttttaaaaat aatccggaaa acgttagttt tcttgcggct cctatcaaat cggatacgga tgtcttggga gtggtaagcg tttatttcgt tcataaaaaa tacatcgatt taaaaactta tttagatttt ttgcaagtag ttgcatccgt aatttatcaa gcgattcgga ttcaaaaact gatcgacgag gaaaaaaggg agatttcgag agagaacgtt cttctcaaaa gagaattaaa gaataaatat aaatttggtt ctttgatcgg taaatccaaa ccgatggaaa aacttttcga aatgattcat cttgtttccg attctagagc ttctgttttg attactggag aatctggtac cggtaaagaa atgattgcct ctgcaattca ttataattct tctcgcgctg ataagccttt tatcaagatc aactgcgctg caattcctga aaacttactt gagagcgaac tctttggcca taagaaaggt tcttttacgg gagctgtttc ggataaaaaa ggtaagttcg aaatggcaga taccggaaca attttcttgg atgaaatcgg agagatggat ctcaatttac aatctaaact tttgagagtt ctgcaagaga aagagattga agccgtcggt tctgttaaac ctaaaaaaat tgacgtaagg gtaatagcgg cgactaacgc ggatttggaa aaattaattt ctgaaaaaag attcaggccg gacctttttt acagactaaa cgtcgtaaat atggtaactc ctcctcttcg tgaaagatcg gatgacattc cacttttgat caatcacttt attgcaaagt atgccgaaga aaacggtaaa aaaattacgg gagtcacgag agaggctcat aaacttttga tgaattacag ttggcctgga aatgttcgag aacttgaaaa tgtaatagaa cgtgcggttg tactttctca actagaaatg ttggatattc aagatttttc tgagatcaat ggacgtcttc tttacggaga tgaggatttt gatccggaaa ctggggattc tgaatcttct gtagaaattg ccaattctag attctcctcc tcaaatttgg atgcgcttga tggaagggct atcgaagttg tagttggaga agtagaagcc cgtttgatta aatacgcgat gaagaagttc aaatatacaa aaacaagagt tgctaaattt ttaggaatca atcgaaatac tttggataaa aaaatcaaag atctcaaaat cgattattga
Details for LpinA.00529.a.B1.GE41892
HARVESTED ON: data unavailable
SEQUENCED ON: 9/29/2017
EXPECTED MW: 79kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS GYVKPSPLRS IQEVATAMNS TLDPDKLLDL ILERCIQICE VGSGSLMLIN EKENVLDIVT FRGMNPSVRT KVKLKVGEGI TGIVAASGEG MIVSDVTANP HYISIKDDIM SELAVPMIVE DVVIGVISLD SSRKGAFNEE HLEIISTLAN QAAQIFKNLQ IFRQLEQKNK IQQVLIDISR TVTSTLVLQE IFEDIMDRLE KSLNLERGSI VLFEPEKAIL KLEAASGLTA EEMEKGVYLP GEGVTGKVFE TGEPIIIESI ANDENFINRV GNAAHFKNNP ENVSFLAAPI KSDTDVLGVV SVYFVHKKYI DLKTYLDFLQ VVASVIYQAI RIQKLIDEEK REISRENVLL KRELKNKYKF GSLIGKSKPM EKLFEMIHLV SDSRASVLIT GESGTGKEMI ASAIHYNSSR ADKPFIKINC AAIPENLLES ELFGHKKGSF TGAVSDKKGK FEMADTGTIF LDEIGEMDLN LQSKLLRVLQ EKEIEAVGSV KPKKIDVRVI AATNADLEKL ISEKRFRPDL FYRLNVVNMV TPPLRERSDD IPLLINHFIA KYAEENGKKI TGVTREAHKL LMNYSWPGNV RELENVIERA VVLSQLEMLD IQDFSEINGR LLYGDEDFDP ETGDSESSVE IANSRFSSSN LDALDGRAIE VVVGEVEARL IKYAMKKFKY TKTRVAKFLG INRNTLDKKI KDLKIDY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag gatatgtgaa gcctagcccc ttaagatcta tacaagaagt tgctacggca atgaattcta ccttagatcc ggataaactt ctggatttga ttttagaacg ttgcattcag atttgcgaag taggctccgg ctctttgatg ttgattaatg agaaagaaaa cgttttagat atagtgactt ttcgtgggat gaatccttcc gttcgaacta aggtaaaact gaaagttgga gaaggaatca ccggaattgt agccgcgtcg ggcgaaggta tgatcgtaag tgatgttacc gctaatccac attacatttc cataaaggat gatattatgt cggaacttgc ggttccgatg attgttgaag acgtagtgat cggagtaatt tctttggact ccagtcgtaa aggagctttc aacgaagaac atttagaaat tatttctact cttgctaatc aagctgccca gatttttaag aacttacaaa tttttagaca actcgaacaa aaaaataaaa ttcaacaagt gctaattgat atttccagaa ctgttacttc cactttggtt ctacaggaaa tttttgaaga tattatggat cgtttggaaa aatccctaaa tttagaacgc ggaagtattg ttcttttcga acctgaaaaa gcgattttga aattggaagc agcctccggt ttaactgcgg aagaaatgga aaaaggggtt tatcttccag gagaaggtgt tactggaaag gtttttgaaa caggggaacc aattatcatc gaatctattg caaatgacga aaattttata aaccgagttg gaaatgcggc tcattttaaa aataatccgg aaaacgttag ttttcttgcg gctcctatca aatcggatac ggatgtcttg ggagtggtaa gcgtttattt cgttcataaa aaatacatcg atttaaaaac ttatttagat tttttgcaag tagttgcatc cgtaatttat caagcgattc ggattcaaaa actgatcgac gaggaaaaaa gggagatttc gagagagaac gttcttctca aaagagaatt aaagaataaa tataaatttg gttctttgat cggtaaatcc aaaccgatgg aaaaactttt cgaaatgatt catcttgttt ccgattctag agcttctgtt ttgattactg gagaatctgg taccggtaaa gaaatgattg cctctgcaat tcattataat tcttctcgcg ctgataagcc ttttatcaag atcaactgcg ctgcaattcc tgaaaactta cttgagagcg aactctttgg ccataagaaa ggttctttta cgggagctgt ttcggataaa aaaggtaagt tcgaaatggc agataccgga acaattttct tggatgaaat cggagagatg gatctcaatt tacaatctaa acttttgaga gttctgcaag agaaagagat tgaagccgtc ggttctgtta aacctaaaaa aattgacgta agggtaatag cggcgactaa cgcggatttg gaaaaattaa tttctgaaaa aagattcagg ccggaccttt tttacagact aaacgtcgta aatatggtaa ctcctcctct tcgtgaaaga tcggatgaca ttccactttt gatcaatcac tttattgcaa agtatgccga agaaaacggt aaaaaaatta cgggagtcac gagagaggct cataaacttt tgatgaatta cagttggcct ggaaatgttc gagaacttga aaatgtaata gaacgtgcgg ttgtactttc tcaactagaa atgttggata ttcaagattt ttctgagatc aatggacgtc ttctttacgg agatgaggat tttgatccgg aaactgggga ttctgaatct tctgtagaaa ttgccaattc tagattctcc tcctcaaatt tggatgcgct tgatggaagg gctatcgaag ttgtagttgg agaagtagaa gcccgtttga ttaaatacgc gatgaagaag ttcaaatata caaaaacaag agttgctaaa tttttaggaa tcaatcgaaa tactttggat aaaaaaatca aagatctcaa aatcgattat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat