LpinA.00483.a

Glutamate 5-kinase (EC 2.7.2.11) (Gamma-glutamyl kinase) (GK)

CENTER ID: LpinA.00483.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.00483.a.B1.GE41891 full length 1 290
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.00483.a.B1.PS38492 full length 1 290

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.3338
UniProt: Q72NQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPERNSLSE KIRNAEKIVI KVGSARLSGL PSEVNDFLFQ LVSDIRHLRD LGKKVILVSS GAIARGRLLL SELPSTISSG DSLAEKQALA AMGQNRLVNL YDSFFSKVNL SIAQILFGVL DLESKEGYKN LKNTFTQLVE WGILPVVNEN DSVATEEVKF GDNDMLSALV SLIVEADLLI ILTGVDGFLK EEKVVPFLEK ISKEDLGLAG GPSGPGTGGM FTKLKSAGLL SEAGIPTAIL NGKKMHVIRE FLEKNSIGTL VAPSGNRVFS EEDVKEIIRK NRNGNGGNSL
NT Sequence
atgaaaccgg aacgaaactc actttctgaa aagatccgta acgctgaaaa gatcgttatc aaagtaggtt cggcaaggct ttctggactt ccgtcggaag taaacgattt tctatttcaa ttggtaagtg atattcgtca tcttagagat ttaggaaaaa aggtgatttt agtctcttca ggagcgattg caagaggaag acttttgtta agcgaacttc cctctacaat ttcttcgggg gattcacttg ccgaaaaaca agcgttagcc gcaatggggc aaaataggct tgtcaatctc tacgatagtt ttttttccaa agtcaatttg agcatcgcac agatcctttt tggtgttttg gatttggaat ccaaagaagg ttataaaaat ctaaagaaca cgtttactca acttgtagaa tgggggatac ttccagtagt taacgaaaat gattccgttg caaccgaaga agtaaagttt ggagacaacg atatgctttc cgctttggtg agtttgatcg tagaagccga tcttttgatt atacttacag gtgtggatgg ttttttaaaa gaagagaaag ttgtaccttt tctggagaaa atttctaaag aagatttagg tcttgctggg ggtcctagtg gacctggcac aggcggaatg ttcactaaac ttaaatctgc gggtttatta tctgaggctg ggattccgac cgcgatccta aacggaaaaa aaatgcatgt gattcgtgaa tttttagaaa aaaattcaat aggaacatta gttgctcctt ccggaaaccg cgttttttct gaagaggacg taaaagaaat catccgtaaa aatagaaacg gaaacggagg aaatagttta tga
Details for LpinA.00483.a.B1.GE41891
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK PERNSLSEKI RNAEKIVIKV GSARLSGLPS EVNDFLFQLV SDIRHLRDLG KKVILVSSGA IARGRLLLSE LPSTISSGDS LAEKQALAAM GQNRLVNLYD SFFSKVNLSI AQILFGVLDL ESKEGYKNLK NTFTQLVEWG ILPVVNENDS VATEEVKFGD NDMLSALVSL IVEADLLIIL TGVDGFLKEE KVVPFLEKIS KEDLGLAGGP SGPGTGGMFT KLKSAGLLSE AGIPTAILNG KKMHVIREFL EKNSIGTLVA PSGNRVFSEE DVKEIIRKNR XXNGGNSL
Validated NT Sequence
cacttataaa ctatttcctc cgtttcnntt tctattttta cggatgattt cttttacgtc ctcttcagaa aaaacgcggt ttccggaagg agcaactaat gttcctattg aatttttttc taaaaattca cgaatcacat gcattttttt tccgtttagg atcgcggtcg gaatcccagc ctcagataat aaacccgcag atttaagttt agtgaacatt ccgcctgtgc caggtccact aggaccccca gcaagaccta aatcttcttt agaaattttc tccagaaaag gtacaacttt ctcttctttt aaaaaaccat ccacacctgt aagtataatc aaaagatcgg cttctacgat caaactcacc aaagcggaaa gcatatcgtt gtctccaaac tttacttctt cggttgcaac ggaatcattt tcgttaacta ctggaagtat cccccattct acaagttgag taaacgtgtt ctttagattt ttataacctt ctttggattc caaatccaaa acaccaaaaa ggatctgtgc gatgctcaaa ttgactttgg aaaaaaaact atcgtagaga ttgacaagcc tattttgccc cattgcggct aacgcttgtt tttcggcaag tgaatccccc gaagaaattg tagagggaag ttcgcttaac aaaagtcttc ctcttgcaat cgctcctgaa gagactaaaa tcaccttttt tcctaaatct ctaagatgac gaatatcact taccaattga aatagaaaat cgtttacttc cgacggaagt ccagaaagcc ttgccgaacc tactttgata acgatctttt cagcgttacg gatcttttca gaaagtgagt ttcgttccgg tttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMK PERNSLSEKI RNAEKIVIKV GSARLSGLPS EVNDFLFQLV SDIRHLRDLG KKVILVSSGA IARGRLLLSE LPSTISSGDS LAEKQALAAM GQNRLVNLYD SFFSKVNLSI AQILFGVLDL ESKEGYKNLK NTFTQLVEWG ILPVVNENDS VATEEVKFGD NDMLSALVSL IVEADLLIIL TGVDGFLKEE KVVPFLEKIS KEDLGLAGGP SGPGTGGMFT KLKSAGLLSE AGIPTAILNG KKMHVIREFL EKNSIGTLVA PSGNRVFSEE DVKEIIRKNR NGNGGNSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac cggaacgaaa ctcactttct gaaaagatcc gtaacgctga aaagatcgtt atcaaagtag gttcggcaag gctttctgga cttccgtcgg aagtaaacga ttttctattt caattggtaa gtgatattcg tcatcttaga gatttaggaa aaaaggtgat tttagtctct tcaggagcga ttgcaagagg aagacttttg ttaagcgaac ttccctctac aatttcttcg ggggattcac ttgccgaaaa acaagcgtta gccgcaatgg ggcaaaatag gcttgtcaat ctctacgata gttttttttc caaagtcaat ttgagcatcg cacagatcct ttttggtgtt ttggatttgg aatccaaaga aggttataaa aatctaaaga acacgtttac tcaacttgta gaatggggga tacttccagt agttaacgaa aatgattccg ttgcaaccga agaagtaaag tttggagaca acgatatgct ttccgctttg gtgagtttga tcgtagaagc cgatcttttg attatactta caggtgtgga tggtttttta aaagaagaga aagttgtacc ttttctggag aaaatttcta aagaagattt aggtcttgct gggggtccta gtggacctgg cacaggcgga atgttcacta aacttaaatc tgcgggttta ttatctgagg ctgggattcc gaccgcgatc ctaaacggaa aaaaaatgca tgtgattcgt gaatttttag aaaaaaattc aataggaaca ttagttgctc cttccggaaa ccgcgttttt tctgaagagg acgtaaaaga aatcatccgt aaaaatagaa acggaaacgg aggaaatagt ttatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LpinA.00483.a.B1.PS38492
PURIFICATION DATe: 8/21/2018
CONCENTRATION: 39.05mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK PERNSLSEKI RNAEKIVIKV GSARLSGLPS EVNDFLFQLV SDIRHLRDLG KKVILVSSGA IARGRLLLSE LPSTISSGDS LAEKQALAAM GQNRLVNLYD SFFSKVNLSI AQILFGVLDL ESKEGYKNLK NTFTQLVEWG ILPVVNENDS VATEEVKFGD NDMLSALVSL IVEADLLIIL TGVDGFLKEE KVVPFLEKIS KEDLGLAGGP SGPGTGGMFT KLKSAGLLSE AGIPTAILNG KKMHVIREFL EKNSIGTLVA PSGNRVFSEE DVKEIIRKNR XXNGGNSL
Validated NT Sequence
cacttataaa ctatttcctc cgtttcnntt tctattttta cggatgattt cttttacgtc ctcttcagaa aaaacgcggt ttccggaagg agcaactaat gttcctattg aatttttttc taaaaattca cgaatcacat gcattttttt tccgtttagg atcgcggtcg gaatcccagc ctcagataat aaacccgcag atttaagttt agtgaacatt ccgcctgtgc caggtccact aggaccccca gcaagaccta aatcttcttt agaaattttc tccagaaaag gtacaacttt ctcttctttt aaaaaaccat ccacacctgt aagtataatc aaaagatcgg cttctacgat caaactcacc aaagcggaaa gcatatcgtt gtctccaaac tttacttctt cggttgcaac ggaatcattt tcgttaacta ctggaagtat cccccattct acaagttgag taaacgtgtt ctttagattt ttataacctt ctttggattc caaatccaaa acaccaaaaa ggatctgtgc gatgctcaaa ttgactttgg aaaaaaaact atcgtagaga ttgacaagcc tattttgccc cattgcggct aacgcttgtt tttcggcaag tgaatccccc gaagaaattg tagagggaag ttcgcttaac aaaagtcttc ctcttgcaat cgctcctgaa gagactaaaa tcaccttttt tcctaaatct ctaagatgac gaatatcact taccaattga aatagaaaat cgtttacttc cgacggaagt ccagaaagcc ttgccgaacc tactttgata acgatctttt cagcgttacg gatcttttca gaaagtgagt ttcgttccgg tttcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMK PERNSLSEKI RNAEKIVIKV GSARLSGLPS EVNDFLFQLV SDIRHLRDLG KKVILVSSGA IARGRLLLSE LPSTISSGDS LAEKQALAAM GQNRLVNLYD SFFSKVNLSI AQILFGVLDL ESKEGYKNLK NTFTQLVEWG ILPVVNENDS VATEEVKFGD NDMLSALVSL IVEADLLIIL TGVDGFLKEE KVVPFLEKIS KEDLGLAGGP SGPGTGGMFT KLKSAGLLSE AGIPTAILNG KKMHVIREFL EKNSIGTLVA PSGNRVFSEE DVKEIIRKNR NGNGGNSL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC CGGAACGAAA CTCACTTTCT GAAAAGATCC GTAACGCTGA AAAGATCGTT ATCAAAGTAG GTTCGGCAAG GCTTTCTGGA CTTCCGTCGG AAGTAAACGA TTTTCTATTT CAATTGGTAA GTGATATTCG TCATCTTAGA GATTTAGGAA AAAAGGTGAT TTTAGTCTCT TCAGGAGCGA TTGCAAGAGG AAGACTTTTG TTAAGCGAAC TTCCCTCTAC AATTTCTTCG GGGGATTCAC TTGCCGAAAA ACAAGCGTTA GCCGCAATGG GGCAAAATAG GCTTGTCAAT CTCTACGATA GTTTTTTTTC CAAAGTCAAT TTGAGCATCG CACAGATCCT TTTTGGTGTT TTGGATTTGG AATCCAAAGA AGGTTATAAA AATCTAAAGA ACACGTTTAC TCAACTTGTA GAATGGGGGA TACTTCCAGT AGTTAACGAA AATGATTCCG TTGCAACCGA AGAAGTAAAG TTTGGAGACA ACGATATGCT TTCCGCTTTG GTGAGTTTGA TCGTAGAAGC CGATCTTTTG ATTATACTTA CAGGTGTGGA TGGTTTTTTA AAAGAAGAGA AAGTTGTACC TTTTCTGGAG AAAATTTCTA AAGAAGATTT AGGTCTTGCT GGGGGTCCTA GTGGACCTGG CACAGGCGGA ATGTTCACTA AACTTAAATC TGCGGGTTTA TTATCTGAGG CTGGGATTCC GACCGCGATC CTAAACGGAA AAAAAATGCA TGTGATTCGT GAATTTTTAG AAAAAAATTC AATAGGAACA TTAGTTGCTC CTTCCGGAAA CCGCGTTTTT TCTGAAGAGG ACGTAAAAGA AATCATCCGT AAAAATAGAA ACGGAAACGG AGGAAATAGT TTATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT