LpinA.00107.a

Heavy metal efflux pump

CENTER ID:	LpinA.00107.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.00107.a.B2.GE41952	mature protein		33	333

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.00107.a.B2.PS38458	mature protein		33	333

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_2677

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|267671.5.peg.2315
UniProt:	Q72R12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIHFTSRTL LITGAAIVIA IVSLAILGLS NRVDKKTKLP PSKPIISENG EKIEFKENSP GLEIIQTKEI GKDGEFINVD APARLIASTS PSVSGGERII LFESAELNDL YVGYVHSRNS LVRSIKNFER ITDMFKHRVA TEKDLIEAET QRNNDQAELA EFEGKLRAVG LNPALLGKSG AQSAWIISDV PESQLSKLKK GKRVKVRFSS FPNEEWSGTA EALGDNVDPM TRTVKVRIVI SNAGYKLKPG MFANVKFPED TANDTVVVPF NAIVTVEGQN YVFVEEAPHE FVRREVTLGI STTDRVNVIE GLTKGDRVVV QGSILLKGLS FGF

NT Sequence

atgaagatac attttacaag tagaacactt ttaattaccg gagcggcgat agtcatcgca attgtttctt tggcgatttt gggactttcc aatcgagtag ataaaaagac aaaacttcct ccgagcaaac cgatcatttc agaaaacgga gaaaaaatcg aatttaaaga aaatagtcca ggtcttgaaa ttatacaaac aaaagaaatt ggaaaggatg gagagtttat caacgtagac gctcctgcgc gtctaattgc ttctacttct ccttctgtga gtggaggaga aaggattatt ctttttgaat ctgccgaact taacgatctc tatgtaggtt atgttcactc tcgtaatagt ttggttcgtt caattaaaaa cttcgaacgg attacagata tgtttaaaca tagagtggca accgaaaagg atcttatcga agccgaaact caaaggaaca atgatcaggc cgagctggct gagtttgaag ggaagttacg tgcagtcggt ttaaacccgg ctctacttgg taaatccgga gctcagagcg cttggatcat tagtgatgtt cctgaatctc aactttctaa acttaaaaaa ggaaaaaggg taaaggttcg tttttcctct tttccaaacg aagaatggtc cggaaccgcg gaagctctcg gcgacaacgt agatcctatg actagaaccg ttaaggttcg gatcgtgatt tctaatgcag gttacaaact caaaccgggt atgtttgcca atgttaagtt tccggaagac acggcgaacg acacggttgt ggttccgttt aacgcgattg ttacagtaga aggccagaac tatgtttttg tagaagaggc tccgcatgag tttgtaagac gcgaagtaac tttaggcatt tcgactacag atcgagtgaa cgttattgaa ggtttgacca aaggcgatcg agttgtcgtt caaggttcta ttcttttaaa gggactcagt tttggttttt aa

Details for LpinA.00107.a.B2.GE41952

HARVESTED ON:	10/3/2017
SEQUENCED ON:	10/12/2017
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV DKKTKLPPSK PIISENGEKI EFKENSPGLE IIQTKEIGKD GEFINVDAPA RLIASTSPSV SGGERIILFE SAELNDLYVG YVHSRNSLVR SIKNFERITD MFKHRVATEK DLIEAETQRN NDQAELAEFE GKLRAVGLNP ALLGKSGAQS AWIISDVPES QLSKLKKGKR VKVRFSSFPN EEWSGTAEAL GDNVDPMTRT VKVRIVISNA GYKLKPGMFA NVKFPEDTAN DTVVVPFNAI VTVEGQNYVF VEEAPHEFVR REVTLGISTT DRVNVIEGLT KGDRVVVQGS IXXKGLSFGF

Validated NT Sequence

atcttactca cttaaaaacc aaaactgagt cccttnnnnn naatagaacc ttgaacgaca actcgatcgc ctttggtcaa accttcaata acgttcactc gatctgtagt cgaaatgcct aaagttactt cgcgtcttac aaactcatgc ggagcctctt ctacaaaaac atagttctgg ccttctactg taacaatcgc gttaaacgga accacaaccg tgtcgttcgc cgtgtcttcc ggaaacttaa cattggcaaa catacccggt ttgagtttgt aacctgcatt agaaatcacg atccgaacct taacggttct agtcatagga tctacgttgt cgccgagagc ttccgcggtt ccggaccatt cttcgtttgg aaaagaggaa aaacgaacct ttaccctttt tcctttttta agtttagaaa gttgagattc aggaacatca ctaatgatcc aagcgctctg agctccggat ttaccaagta gagccgggtt taaaccgact gcacgtaact tcccttcaaa ctcagccagc tcggcctgat cattgttcct ttgagtttcg gcttcgataa gatccttttc ggttgccact ctatgtttaa acatatctgt aatccgttcg aagtttttaa ttgaacgaac caaactatta cgagagtgaa cataacctac atagagatcg ttaagttcgg cagattcaaa aagaataatc ctttctcctc cactcacaga aggagaagta gaagcaatta gacgcgcagg agcgtctacg ttgataaact ctccatcctt tccaatttct tttgtttgta taatttcaag acctggacta ttttctttaa attcgatttt ttctccgttt tctgaaatga tcggtttgct cggaggaagt tttgtctttt tatctaccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHVD KKTKLPPSKP IISENGEKIE FKENSPGLEI IQTKEIGKDG EFINVDAPAR LIASTSPSVS GGERIILFES AELNDLYVGY VHSRNSLVRS IKNFERITDM FKHRVATEKD LIEAETQRNN DQAELAEFEG KLRAVGLNPA LLGKSGAQSA WIISDVPESQ LSKLKKGKRV KVRFSSFPNE EWSGTAEALG DNVDPMTRTV KVRIVISNAG YKLKPGMFAN VKFPEDTAND TVVVPFNAIV TVEGQNYVFV EEAPHEFVRR EVTLGISTTD RVNVIEGLTK GDRVVVQGSI LLKGLSFGF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtagata aaaagacaaa acttcctccg agcaaaccga tcatttcaga aaacggagaa aaaatcgaat ttaaagaaaa tagtccaggt cttgaaatta tacaaacaaa agaaattgga aaggatggag agtttatcaa cgtagacgct cctgcgcgtc taattgcttc tacttctcct tctgtgagtg gaggagaaag gattattctt tttgaatctg ccgaacttaa cgatctctat gtaggttatg ttcactctcg taatagtttg gttcgttcaa ttaaaaactt cgaacggatt acagatatgt ttaaacatag agtggcaacc gaaaaggatc ttatcgaagc cgaaactcaa aggaacaatg atcaggccga gctggctgag tttgaaggga agttacgtgc agtcggttta aacccggctc tacttggtaa atccggagct cagagcgctt ggatcattag tgatgttcct gaatctcaac tttctaaact taaaaaagga aaaagggtaa aggttcgttt ttcctctttt ccaaacgaag aatggtccgg aaccgcggaa gctctcggcg acaacgtaga tcctatgact agaaccgtta aggttcggat cgtgatttct aatgcaggtt acaaactcaa accgggtatg tttgccaatg ttaagtttcc ggaagacacg gcgaacgaca cggttgtggt tccgtttaac gcgattgtta cagtagaagg ccagaactat gtttttgtag aagaggctcc gcatgagttt gtaagacgcg aagtaacttt aggcatttcg actacagatc gagtgaacgt tattgaaggt ttgaccaaag gcgatcgagt tgtcgttcaa ggttctattc ttttaaaggg actcagtttt ggtttttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LpinA.00107.a.B2.PS38458

PURIFICATION DATe:	6/12/2018
CONCENTRATION:	39.48mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTAGATA AAAAGACAAA ACTTCCTCCG AGCAAACCGA TCATTTCAGA AAACGGAGAA AAAATCGAAT TTAAAGAAAA TAGTCCAGGT CTTGAAATTA TACAAACAAA AGAAATTGGA AAGGATGGAG AGTTTATCAA CGTAGACGCT CCTGCGCGTC TAATTGCTTC TACTTCTCCT TCTGTGAGTG GAGGAGAAAG GATTATTCTT TTTGAATCTG CCGAACTTAA CGATCTCTAT GTAGGTTATG TTCACTCTCG TAATAGTTTG GTTCGTTCAA TTAAAAACTT CGAACGGATT ACAGATATGT TTAAACATAG AGTGGCAACC GAAAAGGATC TTATCGAAGC CGAAACTCAA AGGAACAATG ATCAGGCCGA GCTGGCTGAG TTTGAAGGGA AGTTACGTGC AGTCGGTTTA AACCCGGCTC TACTTGGTAA ATCCGGAGCT CAGAGCGCTT GGATCATTAG TGATGTTCCT GAATCTCAAC TTTCTAAACT TAAAAAAGGA AAAAGGGTAA AGGTTCGTTT TTCCTCTTTT CCAAACGAAG AATGGTCCGG AACCGCGGAA GCTCTCGGCG ACAACGTAGA TCCTATGACT AGAACCGTTA AGGTTCGGAT CGTGATTTCT AATGCAGGTT ACAAACTCAA ACCGGGTATG TTTGCCAATG TTAAGTTTCC GGAAGACACG GCGAACGACA CGGTTGTGGT TCCGTTTAAC GCGATTGTTA CAGTAGAAGG CCAGAACTAT GTTTTTGTAG AAGAGGCTCC GCATGAGTTT GTAAGACGCG AAGTAACTTT AGGCATTTCG ACTACAGATC GAGTGAACGT TATTGAAGGT TTGACCAAAG GCGATCGAGT TGTCGTTCAA GGTTCTATTC TTTTAAAGGG ACTCAGTTTT GGTTTTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T