LpinA.00085.a

Oxidoreductase

CENTER ID: LpinA.00085.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.00085.a.B1.GE41890 full length 1 321
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.229
UniProt: Q72VU3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKCLITGAGG FVGSYLLKEL KRSYTDFLGI GIQSGPNITE DLELPKSYRS VVCDIRNLNQ VCSIIHEFSP DVIFHLAAQP FVPKAVEDPG ETLEINIHGT LNLLESLRSL KKKVRFVYIS SSDVYGNVPE SYLPVTESVV PAPLNPYSSS KYCAEIYCLQ YHRWIQDLEI VIARPFNHTG PKQGLNFVIP NFCSQVLKAL KRPESERKIL VGDLSSSRDF LDVRDVVCAY RILAERGKPG EIYNICSGKE VIIRDVLDGI ISTSGQKIPV EVDSTRFRPA EMRRLFGDNQ KLRQLGWEPR FELLDTIQDI YNSIEHSSLI Y
NT Sequence
atgaaatgcc taataacggg agcgggaggt tttgttggta gttatctact aaaagaatta aaacgatctt ataccgattt tttaggaata ggcattcaat ccggtcctaa tataacagaa gatcttgaac ttccgaaatc atatcgctcc gttgtttgtg atatacggaa tttaaaccag gtttgttcta tcatccatga attttcaccg gatgttatat ttcatcttgc ggcacaacct tttgttccaa aagcggtaga agatccaggc gagactttgg agatcaacat acatggaacg ttgaatctat tagaatcttt gcgttcttta aaaaagaaag ttcgtttcgt ttatatttct tcttccgatg tttatggaaa cgttccggaa tcttatcttc cagttacaga atcggtcgtc cctgctcctc tcaatccgta ttcttcttct aaatactgtg cggaaattta ttgtctacaa tatcatagat ggattcaaga tcttgaaatt gtaattgcaa gacctttcaa tcatactggt ccaaagcaag gtttgaattt tgtgattcct aatttttgtt ctcaagtttt aaaagcgctt aaaagacccg aatcggaaag aaaaatttta gtaggagatc tttcgtcttc gagagatttt ttagatgtaa gagacgttgt atgcgcgtat cgaattcttg cagaaagagg gaaaccaggt gaaatttata atatttgttc tggtaaggaa gtgatcatca gagatgtgtt agatggaatc atttctactt caggacagaa gattccggta gaagtcgact ctactcgttt tcgccctgcg gaaatgaggc gcctttttgg agacaatcag aaacttaggc aacttggttg ggaacctcgt ttcgaattat tggatacaat tcaagatatt tataatagca ttgagcatag tagcttaata tactag
Details for LpinA.00085.a.B1.GE41890
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK CLITGAGGFV GSYLLKELKR SYTDFLGIGI QSGPNITEDL ELPKSYRSVV CDIRNLNQVC SIIHEFSPDV IFHLAAQPFV PKAVEDPGET LEINIHGTLN LLESLRSLKK KVRFVYISSS DVYGNVPESY LPVTESVVPA PLNPYSSSKY CAEIYCLQYH RWIQDLEIVI ARPFNHTGPK QGLNFVIPNF CSQVLKALKR PESERKILVG DLSSSRDFLD VRDVVCAYRI LAERGKPGEI YNICSGKEVI IRDVLDGIIS TSGQKIPVEV DSTRFRPAEM RRLFGDNQKL RQLGWEPRFE LLDTIQDIYN XXXHSSLIY
Validated NT Sequence
gatcctatct tactcactta gtatattaag ctactatgnn nnnnnntatt ataaatatct tgaattgtat ccaataattc gaaacgaggt tcccaaccaa gttgcctaag tttctgattg tctccaaaaa ggcgcctcat ttccgcaggg cgaaaacgag tagagtcgac ttctaccgga atcttctgtc ctgaagtaga aatgattcca tctaacacat ctctgatgat cacttcctta ccagaacaaa tattataaat ttcacctggt ttccctcttt ctgcaagaat tcgatacgcg catacaacgt ctcttacatc taaaaaatct ctcgaagacg aaagatctcc tactaaaatt tttctttccg attcgggtct tttaagcgct tttaaaactt gagaacaaaa attaggaatc acaaaattca aaccttgctt tggaccagta tgattgaaag gtcttgcaat tacaatttca agatcttgaa tccatctatg atattgtaga caataaattt ccgcacagta tttagaagaa gaatacggat tgagaggagc agggacgacc gattctgtaa ctggaagata agattccgga acgtttccat aaacatcgga agaagaaata taaacgaaac gaactttctt ttttaaagaa cgcaaagatt ctaatagatt caacgttcca tgtatgttga tctccaaagt ctcgcctgga tcttctaccg cttttggaac aaaaggttgt gccgcaagat gaaatataac atccggtgaa aattcatgga tgatagaaca aacctggttt aaattccgta tatcacaaac aacggagcga tatgatttcg gaagttcaag atcttctgtt atattaggac cggattgaat gcctattcct aaaaaatcgg tataagatcg ttttaattct tttagtagat aactaccaac aaaacctccc gctcccgtta ttaggcattt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMK CLITGAGGFV GSYLLKELKR SYTDFLGIGI QSGPNITEDL ELPKSYRSVV CDIRNLNQVC SIIHEFSPDV IFHLAAQPFV PKAVEDPGET LEINIHGTLN LLESLRSLKK KVRFVYISSS DVYGNVPESY LPVTESVVPA PLNPYSSSKY CAEIYCLQYH RWIQDLEIVI ARPFNHTGPK QGLNFVIPNF CSQVLKALKR PESERKILVG DLSSSRDFLD VRDVVCAYRI LAERGKPGEI YNICSGKEVI IRDVLDGIIS TSGQKIPVEV DSTRFRPAEM RRLFGDNQKL RQLGWEPRFE LLDTIQDIYN SIEHSSLIY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat gcctaataac gggagcggga ggttttgttg gtagttatct actaaaagaa ttaaaacgat cttataccga ttttttagga ataggcattc aatccggtcc taatataaca gaagatcttg aacttccgaa atcatatcgc tccgttgttt gtgatatacg gaatttaaac caggtttgtt ctatcatcca tgaattttca ccggatgtta tatttcatct tgcggcacaa ccttttgttc caaaagcggt agaagatcca ggcgagactt tggagatcaa catacatgga acgttgaatc tattagaatc tttgcgttct ttaaaaaaga aagttcgttt cgtttatatt tcttcttccg atgtttatgg aaacgttccg gaatcttatc ttccagttac agaatcggtc gtccctgctc ctctcaatcc gtattcttct tctaaatact gtgcggaaat ttattgtcta caatatcata gatggattca agatcttgaa attgtaattg caagaccttt caatcatact ggtccaaagc aaggtttgaa ttttgtgatt cctaattttt gttctcaagt tttaaaagcg cttaaaagac ccgaatcgga aagaaaaatt ttagtaggag atctttcgtc ttcgagagat tttttagatg taagagacgt tgtatgcgcg tatcgaattc ttgcagaaag agggaaacca ggtgaaattt ataatatttg ttctggtaag gaagtgatca tcagagatgt gttagatgga atcatttcta cttcaggaca gaagattccg gtagaagtcg actctactcg ttttcgccct gcggaaatga ggcgcctttt tggagacaat cagaaactta ggcaacttgg ttgggaacct cgtttcgaat tattggatac aattcaagat atttataata gcattgagca tagtagctta atatactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t