LpboA.19883.a

Penicillin binding protein/Beta-lactamase class C protein

CENTER ID: LpboA.19883.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19883.a.B2.GE41951 mature protein 37 402
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.811
UniProt: Q04UX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSVILKQSF RKMNMTRKFK LISLFFVVWF IVTCSPVGHK IVDREKRIHD HLLKRAGKND CPGIQYSVTT SKGLIYSSSV GSADIEGNVP LAKDSQMMLH SMTKTFTAAA VLRLIDKGKL SLDDPARKYL PYIPYDERVT IRNLLSQTSG IPDPIPLRWV HTAKEDDSFN ENRILREILE GHSKLNFEPG KKYAYSNISY WLLGKIVEKV SGVPYKEYMI SEIFKKLNIS DSEMGFLIPT GTKHVKGYLR KFSFFNLLKI FLLDSKFFGK YEGDWLHVRD HYLNGPPFGG IVSNAESVSV FLRDQLRNDS VLFSKDTKDL FYQQQNDNTG KPVEMSLGWH IRSLNGNRYY FKEGGGAGFH SEMRIYPELR IATVAISNNT EFNTRDFLNE TDPEFFSSET PR
NT Sequence
ttgaaatccg tcatattgaa acagtctttt cgaaaaatga acatgacccg taaattcaaa ttgatctccc tcttttttgt ggtttggttt attgtaacgt gcagtccggt aggtcataaa atagtggata gggaaaaaag gatacacgat catcttttga agcgcgcagg aaagaatgat tgccccggta tacaatattc ggtaacgact tcgaaaggcc tgatttatag ttcaagtgtg ggatcagcgg atatagaagg aaatgtacct ttggctaagg actcacaaat gatgctccat tctatgacga aaacctttac ggcggcggct gttctccgat tgatagataa gggaaaattg tctctcgacg atcctgcacg caaatatcta ccgtatatac cctacgatga gcgggtaacg atccgaaatt tattgtcgca aacctccggt attcctgatc cgattccttt aagatgggtt cataccgcaa aagaggacga ttcgttcaac gagaaccgaa tactccgcga aatcttggaa ggacattcca aactgaattt cgaaccggga aaaaaatacg cctattccaa catatcctat tggcttctag gcaagattgt ggaaaaggta tccggcgttc cctataaaga atatatgatt tccgaaatat tcaaaaaatt aaatatttcg gattctgaga tgggattttt gattccaacc ggaacgaagc acgtaaaagg atatttaaga aaattctctt tcttcaatct attgaaaatt tttttactgg attccaagtt cttcggcaaa tacgaagggg attggttgca tgtccgagat cattatctaa acggcccgcc tttcggggga atcgtctcga atgccgaatc ggtaagcgtt tttttaaggg atcagctaag gaacgattcc gttctttttt ctaaagatac aaaggatttg ttttatcagc aacaaaacga caacactgga aaacccgtgg aaatgagttt gggttggcac atccgatctt tgaacgggaa taggtattat ttcaaagaag gtggcggagc cggtttccat agcgaaatga ggatttaccc cgaactaagg atagcaaccg tcgcgatttc caacaatacc gaattcaaca ctcgagattt tttaaacgaa acagatcctg aatttttttc gagtgaaact ccccggtga
Details for LpboA.19883.a.B2.GE41951
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMV GHKIVDREKR IHDHLLKRAG KNDCPGIQYS VTTSKGLIYS SSVGSADIEG NVPLAKDSQM MLHSMTKTFT AAAVLRLIDK GKLSLDDPAR KYLPYIPYDE RVTIRNLLSQ TSGIPDPIPL RWVHTAKEDD SFNENRILRE ILEGHSKLNF EPGKKYAYSN ISYWLLGKIV EKVSGVPYKE YMISEIFKKL NISDSEMGFL IPTGTKHVKG YLRKFSFFNL LKIFLLDSKF FGKYEGDWLH VRDHYLNGPP FGGIVSNAES VSVFLRDQLR NDSVLFSKDT KDLFYQQQND NTGKPVEMSL GWHIRSLNGN RYYFKEGGGA GFHSEMRIYP ELRIATVAIS NNTEFNTRDF LNETD
Validated NT Sequence
aggatctgtt tcgtttaaaa aatctcgagt gttgaattcg gtattgttgg aaatcgcgac ggttgctatc cttagttcgg ggtaaatcct catttcgcta tggaaaccgg ctccgccacc ttctttgaaa taatacctat tcccgttcaa agatcggatg tgccaaccca aactcatttc cacgggtttt ccagtgttgt cgttttgttg ctgataaaac aaatcctttg tatctttaga aaaaagaacg gaatcgttcc ttagctgatc ccttaaaaaa acgcttaccg attcggcatt cgagacgatt cccccgaaag gcgggccgtt tagataatga tctcggacat gcaaccaatc cccttcgtat ttgccgaaga acttggaatc cagtaaaaaa attttcaata gattgaagaa agagaatttt cttaaatatc cttttacgtg cttcgttccg gttggaatca aaaatcccat ctcagaatcc gaaatattta attttttgaa tatttcggaa atcatatatt ctttataggg aacgccggat accttttcca caatcttgcc tagaagccaa taggatatgt tggaataggc gtattttttt cccggttcga aattcagttt ggaatgtcct tccaagattt cgcggagtat tcggttctcg ttgaacgaat cgtcctcttt tgcggtatga acccatctta aaggaatcgg atcaggaata ccggaggttt gcgacaataa atttcggatc gttacccgct catcgtaggg tatatacggt agatatttgc gtgcaggatc gtcgagagac aattttccct tatctatcaa tcggagaaca gccgccgccg taaaggtttt cgtcatagaa tggagcatca tttgtgagtc cttagccaaa ggtacatttc cttctatatc cgctgatccc acacttgaac tataaatcag gcctttcgaa gtcgttaccg aatattgtat accggggcaa tcattctttc ctgcgcgctt caaaagatga tcgtgtatcc ttttttccct atccactatt ttatgaccta ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHVG HKIVDREKRI HDHLLKRAGK NDCPGIQYSV TTSKGLIYSS SVGSADIEGN VPLAKDSQMM LHSMTKTFTA AAVLRLIDKG KLSLDDPARK YLPYIPYDER VTIRNLLSQT SGIPDPIPLR WVHTAKEDDS FNENRILREI LEGHSKLNFE PGKKYAYSNI SYWLLGKIVE KVSGVPYKEY MISEIFKKLN ISDSEMGFLI PTGTKHVKGY LRKFSFFNLL KIFLLDSKFF GKYEGDWLHV RDHYLNGPPF GGIVSNAESV SVFLRDQLRN DSVLFSKDTK DLFYQQQNDN TGKPVEMSLG WHIRSLNGNR YYFKEGGGAG FHSEMRIYPE LRIATVAISN NTEFNTRDFL NETDPEFFSS ETPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtaggtc ataaaatagt ggatagggaa aaaaggatac acgatcatct tttgaagcgc gcaggaaaga atgattgccc cggtatacaa tattcggtaa cgacttcgaa aggcctgatt tatagttcaa gtgtgggatc agcggatata gaaggaaatg tacctttggc taaggactca caaatgatgc tccattctat gacgaaaacc tttacggcgg cggctgttct ccgattgata gataagggaa aattgtctct cgacgatcct gcacgcaaat atctaccgta tataccctac gatgagcggg taacgatccg aaatttattg tcgcaaacct ccggtattcc tgatccgatt cctttaagat gggttcatac cgcaaaagag gacgattcgt tcaacgagaa ccgaatactc cgcgaaatct tggaaggaca ttccaaactg aatttcgaac cgggaaaaaa atacgcctat tccaacatat cctattggct tctaggcaag attgtggaaa aggtatccgg cgttccctat aaagaatata tgatttccga aatattcaaa aaattaaata tttcggattc tgagatggga tttttgattc caaccggaac gaagcacgta aaaggatatt taagaaaatt ctctttcttc aatctattga aaattttttt actggattcc aagttcttcg gcaaatacga aggggattgg ttgcatgtcc gagatcatta tctaaacggc ccgcctttcg ggggaatcgt ctcgaatgcc gaatcggtaa gcgttttttt aagggatcag ctaaggaacg attccgttct tttttctaaa gatacaaagg atttgtttta tcagcaacaa aacgacaaca ctggaaaacc cgtggaaatg agtttgggtt ggcacatccg atctttgaac gggaataggt attatttcaa agaaggtggc ggagccggtt tccatagcga aatgaggatt taccccgaac taaggatagc aaccgtcgcg atttccaaca ataccgaatt caacactcga gattttttaa acgaaacaga tcctgaattt ttttcgagtg aaactccccg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat