SSGCID: Target Page

LpboA.19869.a

CRISPR-associated endonuclease Cas1 (EC 3.1.-.-)

CENTER ID:	LpboA.19869.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19869.a.B1.GE41950	full length		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.821
UniProt:	Q04QB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPNRNLQELP KFEDNWSHLY FEKGRIDQFQ KSIGFHYLDK VVPIPIETIG LLLLGPGTSI THEAIKRISD SRCLLAWTGE GGVRFYSAGY TGTYSARNLL RQVEAYGNPK ERVSVIRKMY QFRFSDEIPA DATIEQIRGL EGARVRKIYR EWSENSGVPW KSRSYDQNSW DWSDPINRAL SSANACLYGV VHAAILQSGF SPAIGFVHTG KQLSFVYDIA DLYKAEITIP LAFEITAEDA TNVERRVRFA CRDKFKHSKL LKRIIPDIKE LIYGGFDFGE SEDFSEGRDV AVSH

NT Sequence

atgccgaaca gaaacctaca agaacttccg aaatttgaag acaattggtc gcatttatac tttgaaaagg gtaggatcga ccaatttcag aaatccatcg gttttcatta tctggataaa gtcgttccta ttccgatcga aacgatcggc cttcttttat tgggtccggg aacctcgatc actcacgaag cgatcaaaag gatttcggat agcaggtgtc ttctcgcttg gacgggcgaa ggcggagttc gtttttattc cgccggttat acaggtactt attccgccag aaacttactt cgtcaggtgg aagcttacgg taatccgaag gaaagagtgt cggtgatccg taaaatgtat cagttccgtt tttcggacga aattccagcg gacgctacca tcgaacaaat tcgcggtttg gaaggggcga gggttcgaaa aatttacagg gaatggtccg aaaattccgg agttccttgg aagagcagat cttacgacca aaacagctgg gactggagcg atccgatcaa ccgtgctctt tcttccgcaa acgcttgtct ttacggagtg gttcatgcgg cgattcttca atccggtttt tctccggcta tcggttttgt tcatacgggc aaacagttgt ccttcgtcta cgatattgcg gatctttata aagctgaaat tacgatccct ttggctttcg aaattactgc tgaagacgcg actaacgtag aaagaagggt ccgtttcgcc tgcagagata aattcaaaca tagtaaatta cttaaacgaa ttattccgga tatcaaggag ctgatctatg gtggttttga ttttggagag agtgaagact tctcagaggg gagagatgtc gcggttagcc attga

Details for LpboA.19869.a.B1.GE41950

HARVESTED ON:	10/3/2017
SEQUENCED ON:	10/12/2017
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP NRNLQELPKF EDNWSHLYFE KGRIDQFQKS IGFHYLDKVV PIPIETIGLL LLGPGTSITH EAIKRISDSR CLLAWTGEGG VRFYSAGYTG TYSARNLLRQ VEAYGNPKER VSVIRKMYQF RFSDEIPADA TIEQIRGLEG ARVRKIYREW SENSGVPWKS RSYDQNSWDW SDPINRALSS ANACLYGVVH AAILQSGFSP AIGFVHTGKQ LSFVYDIADL YKAEITIPLA FEITAEDATN VERRVRFACR DKFKHSKLLK RIIPDIKELI YGGFDFGESE DFSXXXDVAV SH

Validated NT Sequence

ttactcactt aatggctaac cgcgacatct nnnnnnnctg agaagtcttc actctctcca aaatcaaaac caccatagat cagctccttg atatccggaa taattcgttt aagtaattta ctatgtttga atttatctct gcaggcgaaa cggacccttc tttctacgtt agtcgcgtct tcagcagtaa tttcgaaagc caaagggatc gtaatttcag ctttataaag atccgcaata tcgtagacga aggacaactg tttgcccgta tgaacaaaac cgatagccgg agaaaaaccg gattgaagaa tcgccgcatg aaccactccg taaagacaag cgtttgcgga agaaagagca cggttgatcg gatcgctcca gtcccagctg ttttggtcgt aagatctgct cttccaagga actccggaat tttcggacca ttccctgtaa atttttcgaa ccctcgcccc ttccaaaccg cgaatttgtt cgatggtagc gtccgctgga atttcgtccg aaaaacggaa ctgatacatt ttacggatca ccgacactct ttccttcgga ttaccgtaag cttccacctg acgaagtaag tttctggcgg aataagtacc tgtataaccg gcggaataaa aacgaactcc gccttcgccc gtccaagcga gaagacacct gctatccgaa atccttttga tcgcttcgtg agtgatcgag gttcccggac ccaataaaag aaggccgatc gtttcgatcg gaataggaac gactttatcc agataatgaa aaccgatgga tttctgaaat tggtcgatcc tacccttttc aaagtataaa tgcgaccaat tgtcttcaaa tttcggaagt tcttgtaggt ttctgttcgg catatggtgg tggtggtggt gagccatggt ata

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccga acagaaacct acaagaactt ccgaaatttg aagacaattg gtcgcattta tactttgaaa agggtaggat cgaccaattt cagaaatcca tcggttttca ttatctggat aaagtcgttc ctattccgat cgaaacgatc ggccttcttt tattgggtcc gggaacctcg atcactcacg aagcgatcaa aaggatttcg gatagcaggt gtcttctcgc ttggacgggc gaaggcggag ttcgttttta ttccgccggt tatacaggta cttattccgc cagaaactta cttcgtcagg tggaagctta cggtaatccg aaggaaagag tgtcggtgat ccgtaaaatg tatcagttcc gtttttcgga cgaaattcca gcggacgcta ccatcgaaca aattcgcggt ttggaagggg cgagggttcg aaaaatttac agggaatggt ccgaaaattc cggagttcct tggaagagca gatcttacga ccaaaacagc tgggactgga gcgatccgat caaccgtgct ctttcttccg caaacgcttg tctttacgga gtggttcatg cggcgattct tcaatccggt ttttctccgg ctatcggttt tgttcatacg ggcaaacagt tgtccttcgt ctacgatatt gcggatcttt ataaagctga aattacgatc cctttggctt tcgaaattac tgctgaagac gcgactaacg tagaaagaag ggtccgtttc gcctgcagag ataaattcaa acatagtaaa ttacttaaac gaattattcc ggatatcaag gagctgatct atggtggttt tgattttgga gagagtgaag acttctcaga ggggagagat gtcgcggtta gccattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat