SSGCID: Target Page

LpboA.19844.a

Uncharacterized protein

CENTER ID:	LpboA.19844.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19844.a.B1.GE42888	full length		1	360

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	Q04TF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDHILEIISE YVAIEKPEFA LLINGKWGSG KTYFIKNEVI NNLKSKKAIY LSLNGLGNID IISTRLLISY FNISESINIG HDIVKSLMDM ADSAPYVKIG SNILKGLENL ANKILSKEIN LTDSLIIFDD LERISPTLSI IDILGYINTN FIENHNYSVI FLCDEQKITS AGYQAAKEKV IGRTLEFKHS VDDFIDEYFT DRYSKSSEYL TFLQNHKNVI VEFFLRAKEE NIRTYNFIFD SFKNIFYIVY ESELSEYLLS LLQLFISISI EFKRGVLVSA DSTKFKGLDH LERNVSALLS QRSLLKQIKG EVPNNESPYY EIFSGRYIER SGVPCFLDSF KLRKLKEIYN FYSLVYREGR

NT Sequence

atggaccaca ttttagaaat aatttcagag tatgttgcaa ttgaaaagcc agaatttgca cttttaatca atggaaaatg ggggtctggc aaaacatact ttatcaaaaa tgaagttatt aacaatctga aaagtaagaa agcaatatat ctttccttaa atgggttggg caatatagat atcatatcta caagattgct aatatcttat tttaatattt cagaatctat taatatcggt cacgatattg ttaagtctct aatggatatg gctgattcag ccccttatgt taaaataggt agcaacattt taaaaggact agagaacctt gcaaataaga tcctttcgaa agaaattaat ttaacagatt cgttgataat attcgacgac ttggagagaa tcagtcctac cttaagtatt attgatatct taggctatat aaatactaat ttcattgaaa atcataatta tagcgtaatt tttctctgtg atgagcaaaa aatcacaagc gcaggatatc aagccgcgaa agaaaaagta attgggagaa ctttagagtt taaacactca gtagatgatt ttatagatga atattttact gaccgttatt ctaaatcctc tgaatattta accttcctgc aaaatcataa aaatgtgata gtagaatttt ttctacgggc aaaagaggag aatatacgga cttataattt tattttcgat tcatttaaga atatttttta tattgtttat gaatcagagc tatccgaata tttgctgagt ctattgcagc tatttatttc aatttcaata gaatttaaaa gaggtgtttt agtttctgca gattcaacaa aatttaaagg tttagaccat ttagagcgta atgtttctgc actattatcg caaagatcct tgcttaaaca aataaaaggt gaagttccaa ataatgaatc tccatattat gaaatattct ccggcagata tattgaacgt tccggggtgc cgtgtttttt ggattcgttt aaacttcgta aattgaaaga gatttataac ttttattccc ttgtttatcg agaagggcga taa

Details for LpboA.19844.a.B1.GE42888

HARVESTED ON:	2/7/2019
SEQUENCED ON:	2/8/2019
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMD HILEIISEYV AIEKPEFALL INGKWGSGKT YFIKNEVINN LKSKKAIYLS LNGLGNIDII STRLLISYFN ISESINIGHD IVKSLMDMAD SAPYVKIGSN ILKGLENLAN KILSKEINLT DSLIIFDDLE RISPTLSIID ILGYINTNFI ENHNYSVIFL CDEQKITSAG YQAAKEKVIG RTLEFKHSVD DFIDEYFTDR YSKSSEYLTF LQNHKNVIVE FFLRAKEENI RTYNFIFDSF KNIFYIVYES ELSEYLLSLL QLFISISIEF KRGVLVSADS TKFKGLDHLE RNVSALLSQR SLLKQIKGEV PNNESPYYEI FSGRYIERSG VPCFLDSFKL RKLKEIYNFY SLVYREGR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacc acattttaga aataatttca gagtatgttg caattgaaaa gccagaattt gcacttttaa tcaatggaaa atgggggtct ggcaaaacat actttatcaa aaatgaagtt attaacaatc tgaaaagtaa gaaagcaata tatctttcct taaatgggtt gggcaatata gatatcatat ctacaagatt gctaatatct tattttaata tttcagaatc tattaatatc ggtcacgata ttgttaagtc tctaatggat atggctgatt cagcccctta tgttaaaata ggtagcaaca ttttaaaagg actagagaac cttgcaaata agatcctttc gaaagaaatt aatttaacag attcgttgat aatattcgac gacttggaga gaatcagtcc taccttaagt attattgata tcttaggcta tataaatact aatttcattg aaaatcataa ttatagcgta atttttctct gtgatgagca aaaaatcaca agcgcaggat atcaagccgc gaaagaaaaa gtaattggga gaactttaga gtttaaacac tcagtagatg attttataga tgaatatttt actgaccgtt attctaaatc ctctgaatat ttaaccttcc tgcaaaatca taaaaatgtg atagtagaat tttttctacg ggcaaaagag gagaatatac ggacttataa ttttattttc gattcattta agaatatttt ttatattgtt tatgaatcag agctatccga atatttgctg agtctattgc agctatttat ttcaatttca atagaattta aaagaggtgt tttagtttct gcagattcaa caaaatttaa aggtttagac catttagagc gtaatgtttc tgcactatta tcgcaaagat ccttgcttaa acaaataaaa ggtgaagttc caaataatga atctccatat tatgaaatat tctccggcag atatattgaa cgttccgggg tgccgtgttt tttggattcg tttaaacttc gtaaattgaa agagatttat aacttttatt cccttgttta tcgagaaggg cgatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat