SSGCID: Target Page

LpboA.19797.a

Uncharacterized protein

CENTER ID:	LpboA.19797.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19797.a.B1.GE42157	full length		1	393

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355277.4.peg.3895
UniProt:	Q04NU1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRDWIITLCA NHPGNSSVLT IVDGFCGGGF YLDPESDQFW EGSPIRILRV VESAMREVRE KRGKPRFILN IKVFFIDNED QHTECLKGYL NSLEDNDKFV KFHYQIITKE FSDVLDHCLD DIKKRGGSSF FFVDPFGYTQ FSMLDIQKIM SLNKSEILLT FMIDFIRRFL SNREDSLKSF DNRLDAKGYF LKANIDGIDT LPQQTYLRDE TLRLFRDKGN VRYVYTFGML KTSTIVTYYL IHFANHRRAQ EVIKDTLWRY NNIDLSYQFS YGIYGLGFRT PEYFEESLRL FDIKSENHKQ CVIDMQKGII PLIYEHKDGV RFRDLVDLTI QMNPANLEIY KDFIVKSRED MELKVKRNGK DSKARHLKPD DIIIKFNNKQ YFLFPDMKQF YIK

NT Sequence

ttgcgggact ggatcattac actctgcgcc aatcatcctg gaaattcaag tgttcttacc attgtagatg gcttttgcgg tggcggtttt tatcttgatc ccgaatctga tcagttctgg gaggggtctc caattagaat tcttagggtt gttgagagcg caatgcgtga agttcgtgaa aaacgaggta agccacgttt tattctcaat ataaaagttt tctttataga taatgaagat cagcatactg aatgtttaaa aggttatctc aatagcttgg aggataatga taaattcgtt aaatttcatt atcaaataat aacaaaagaa ttttctgatg tacttgatca ttgtttagac gatataaaaa aaagaggggg cagttctttc ttctttgttg atccttttgg ctatacccaa tttagcatgt tagatataca aaaaattatg tctttgaaca aaagcgaaat actactaact tttatgatag attttattcg aagattttta agtaatagag aggatagtct taagagtttt gataaccgac ttgatgcaaa aggttatttt ttgaaagcta atattgatgg aatagataca ctgccacaac agacctattt gagagatgaa acattaaggt tattccgtga taaaggtaat gttaggtatg tgtatacttt tggaatgctg aaaacttcaa caatcgtaac atattattta attcattttg cgaatcatcg ccgcgctcaa gaagttatta aagataccct ttggcgctac aataatattg atttaagcta tcagttttca tacggcatat atggattagg gtttaggact ccagaatatt ttgaagagtc actgcgatta tttgatataa aatcagaaaa ccataaacaa tgcgttattg atatgcagaa aggaattatt cctctaattt atgaacataa ggatggggtc agatttcgcg atttggtgga tttaactatt caaatgaacc cggccaacct agaaatttat aaggacttta ttgttaaatc tagagaagat atggagttaa aggtaaaaag aaatggtaag gattctaaag caaggcattt gaaaccggat gatattatta taaagtttaa taataagcaa tattttcttt ttcctgatat gaagcagttt tatatcaaat ga

Details for LpboA.19797.a.B1.GE42157

HARVESTED ON:	1/16/2018
SEQUENCED ON:	1/17/2018
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML RDWIITLCAN HPGNSSVLTI VDGFCGGGFY LDPESDQFWE GSPIRILRVV ESAMREVREK RGKPRFILNI KVFFIDNEDQ HTECLKGYLN SLEDNDKFVK FHYQIITKEF SDVLDHCLDD IKKRGGSSFF FVDPFGYTQF SMLDIQKIMS LNKSEILLTF MIDFIRRFLS NREDSLKSFD NRLDAKGYFL KANIDGIDTL PQQTYLRDET LRLFRDKGNV RYVYTFGMLK TSTIVTYYLI HFANHRRAQE VIKDTLWRYN NIDLSYQFSY GIYGLGFRTP EYFEESLRLF DIKSENHKQC VIDMQKGIIP LIYEHKDGVR FRDLVDLTIQ MNPANLEIYK DFIVKSREDM ELKVKRNGKD SKARHLKPDD IIIKFNNKQY FLFP

Validated NT Sequence

ataccatggc tcaccaccac caccaccata tgttgcggga ctggatcatt acactctgcg ccaatcatcc tggaaattca agtgttctta ccattgtaga tggcttttgc ggtggcggtt tttatcttga tcccgaatct gatcagttct gggaggggtc tccaattaga attcttaggg ttgttgagag cgcaatgcgt gaagttcgtg aaaaacgagg taagccacgt tttattctca atataaaagt tttctttata gataatgaag atcagcatac tgaatgttta aaaggttatc tcaatagctt ggaggataat gataaattcg ttaaatttca ttatcaaata ataacaaaag aattttctga tgtacttgat cattgtttag acgatataaa aaaaagaggg ggcagttctt tcttctttgt tgatcctttt ggctataccc aatttagcat gttagatata caaaaaatta tgtctttgaa caaaagcgaa atactactaa cttttatgat agattttatt cgaagatttt taagtaatag agaggatagt cttaagagtt ttgataaccg acttgatgca aaaggttatt ttttgaaagc taatattgat ggaatagata cactgccaca acagacctat ttgagagatg aaacattaag gttattccgt gataaaggta atgttaggta tgtgtatact tttggaatgc tgaaaacttc aacaatcgta acatattatt taattcattt tgcgaatcat cgccgcgctc aagaagttat taaagatacc ctttggcgct acaataatat tgatttaagc tatcagtttt catacggcat atatggatta gggtttagga ctccagaata ttttgaagag tcactgcgat tatttgatat aaaatcagaa aaccataaac aatgcgttat tgatatgcag aaaggaatta ttcctctaat ttatgaacat aaggatgggg tcagatttcg cgatttggtg gatttaacta ttcaaatgaa cccggccaac ctagaaattt ataaggactt tattgttaaa tctagagaag atatggagtt aaaggtaaaa agaaatggta aggattctaa agcaaggcat ttgaaaccgg atgatattat tataaagttt aataataagc aatattttct ttttccng

Expected Protein Sequence

MAHHHHHHMR DWIITLCANH PGNSSVLTIV DGFCGGGFYL DPESDQFWEG SPIRILRVVE SAMREVREKR GKPRFILNIK VFFIDNEDQH TECLKGYLNS LEDNDKFVKF HYQIITKEFS DVLDHCLDDI KKRGGSSFFF VDPFGYTQFS MLDIQKIMSL NKSEILLTFM IDFIRRFLSN REDSLKSFDN RLDAKGYFLK ANIDGIDTLP QQTYLRDETL RLFRDKGNVR YVYTFGMLKT STIVTYYLIH FANHRRAQEV IKDTLWRYNN IDLSYQFSYG IYGLGFRTPE YFEESLRLFD IKSENHKQCV IDMQKGIIPL IYEHKDGVRF RDLVDLTIQM NPANLEIYKD FIVKSREDME LKVKRNGKDS KARHLKPDDI IIKFNNKQYF LFPDMKQFYI K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcggg actggatcat tacactctgc gccaatcatc ctggaaattc aagtgttctt accattgtag atggcttttg cggtggcggt ttttatcttg atcccgaatc tgatcagttc tgggaggggt ctccaattag aattcttagg gttgttgaga gcgcaatgcg tgaagttcgt gaaaaacgag gtaagccacg ttttattctc aatataaaag ttttctttat agataatgaa gatcagcata ctgaatgttt aaaaggttat ctcaatagct tggaggataa tgataaattc gttaaatttc attatcaaat aataacaaaa gaattttctg atgtacttga tcattgttta gacgatataa aaaaaagagg gggcagttct ttcttctttg ttgatccttt tggctatacc caatttagca tgttagatat acaaaaaatt atgtctttga acaaaagcga aatactacta acttttatga tagattttat tcgaagattt ttaagtaata gagaggatag tcttaagagt tttgataacc gacttgatgc aaaaggttat tttttgaaag ctaatattga tggaatagat acactgccac aacagaccta tttgagagat gaaacattaa ggttattccg tgataaaggt aatgttaggt atgtgtatac ttttggaatg ctgaaaactt caacaatcgt aacatattat ttaattcatt ttgcgaatca tcgccgcgct caagaagtta ttaaagatac cctttggcgc tacaataata ttgatttaag ctatcagttt tcatacggca tatatggatt agggtttagg actccagaat attttgaaga gtcactgcga ttatttgata taaaatcaga aaaccataaa caatgcgtta ttgatatgca gaaaggaatt attcctctaa tttatgaaca taaggatggg gtcagatttc gcgatttggt ggatttaact attcaaatga acccggccaa cctagaaatt tataaggact ttattgttaa atctagagaa gatatggagt taaaggtaaa aagaaatggt aaggattcta aagcaaggca tttgaaaccg gatgatatta ttataaagtt taataataag caatattttc tttttcctga tatgaagcag ttttatatca aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat