SSGCID: Target Page

LpboA.19782.a

RNA-directed DNA polymerase

CENTER ID:	LpboA.19782.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19782.a.B1.GE42154	full length		1	309

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.1389
UniProt:	Q04RY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHTVHGNFFY KLSNKKRLAK YLRVSLKELL TLQDDSNYKV WSEEGENGKL REIQEPVYKL KSVHSKIQKS LASIVAPEFL FSGVKGKSNI SNASYHKDGN YIVTADIQSF YINCNKEHIF RFFKYTLRTS DDIARILTEL CCYKTFLPTG SPVSQILAYH SYARIFNRID AFSKANDITF SLYVDDITLS SEKSIHRYIL KTISKLLKSV NLSLKKEKTK FYNKNSYKIV TGCAISPDHI LLRPNKIMRK IDNKLCKHEK DLSKLTPKEI ESVLGQVIYL RSITPNSYPQ LFKSLSLMKT EEKIKQRPL

NT Sequence

atgcacacag ttcacggtaa ctttttttat aaactttcaa acaaaaaaag attagcgaaa tacttacgtg tttccctcaa agaactcctt actctccaag atgattcaaa ctataaagta tggtctgagg aaggagaaaa cggaaaactc agagaaatac aagagcccgt ctataaactc aaatcagtac attcaaaaat tcaaaagtct cttgcttcga ttgtagcgcc agaatttctt ttttcaggag taaaaggaaa atcaaatatt tccaatgcgt catatcataa agatggcaac tatatagtaa cggcggacat tcaaagcttt tacattaact gcaataaaga acacattttt agattcttta aatatacgct tcgcacttca gatgatattg cgcgaatact aactgagctt tgctgctata aaactttcct gccaacaggc agtccggtca gccaaatctt agcctaccat tcttacgcaa gaatattcaa tagaattgat gctttttcaa aagcgaacga tatcactttc tccttatatg tagacgatat tacgctatct tcagaaaaat ctattcatcg ttatatccta aagacaatat caaaacttct aaagtccgta aacttatcat tgaaaaaaga aaaaacaaaa ttttataata aaaactccta caaaatagta acaggatgcg cgattagtcc tgatcacata ctattacgac ctaataaaat tatgagaaag atcgataata aactctgtaa acatgaaaag gatctatcga aattaactcc aaaagaaatc gaaagcgttt taggccaagt gatatattta agatctataa ccccgaattc ttacccccaa ttattcaaat ctttaagctt aatgaaaaca gaagaaaaaa ttaaacagag acccttgtaa

Details for LpboA.19782.a.B1.GE42154

HARVESTED ON:	1/16/2018
SEQUENCED ON:	1/17/2018
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

tcaccaccac caccaccata tgcacacagt tcacggtaac tttttttata aactttcaaa caaaaaaaga ttagcgaaat acttacgtgt ttccctcaaa gaactcctta ctctccaaga tgattcaaac tataaagtat ggtctgagga aggagaaaac ggaaaactca gagaaataca agagcccgtc tataaactca aatcagtaca ttcaaaaatt caaaagtctc ttgcttcgat tgtagcgcca gaatttcttt tttcaggagt aaaaggaaaa tcaaatattt ccaatgcgtc atatcataaa gatggcaact atatagtaac ggcggacatt caaagctttt acattaactg caataaagaa cacattttta gattctttaa atatacgctt cgcacttcag atgatattgc gcgaatacta actgagcttt gctgctataa aactttcctg ccaacaggca gtccggtcag ccaaatctta gcctaccatt cttacgcaag aatattcaat agaattgatg ctttttcaaa agcgaacgat atcactttct ccttatatgt agacgatatt acgctatctt cagaaaaatc tattcatcgt tatatcctaa agacaatatc aaaacttcta aagtccgtaa acttatcatt gaaaaaagaa aaaacaaaat tttataataa aaactcctac aaaatagtaa caggatgcgc gattagtcct gatcacatac tattacgacc taataaaatt atgagaaaga tcgataataa actctgtaaa catgaaaagg atctatcgaa attaactcca aaagaaatcg aaagcgtttt aggccaagtg atatatttaa gatctataac cccgaattct tacccccaat tattcaaatc tttaagctta atgaaaacag aagaannnnt taaacagaga cccttgtaag tgagtaa

Expected Protein Sequence

MAHHHHHHMH TVHGNFFYKL SNKKRLAKYL RVSLKELLTL QDDSNYKVWS EEGENGKLRE IQEPVYKLKS VHSKIQKSLA SIVAPEFLFS GVKGKSNISN ASYHKDGNYI VTADIQSFYI NCNKEHIFRF FKYTLRTSDD IARILTELCC YKTFLPTGSP VSQILAYHSY ARIFNRIDAF SKANDITFSL YVDDITLSSE KSIHRYILKT ISKLLKSVNL SLKKEKTKFY NKNSYKIVTG CAISPDHILL RPNKIMRKID NKLCKHEKDL SKLTPKEIES VLGQVIYLRS ITPNSYPQLF KSLSLMKTEE KIKQRPL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaca cagttcacgg taactttttt tataaacttt caaacaaaaa aagattagcg aaatacttac gtgtttccct caaagaactc cttactctcc aagatgattc aaactataaa gtatggtctg aggaaggaga aaacggaaaa ctcagagaaa tacaagagcc cgtctataaa ctcaaatcag tacattcaaa aattcaaaag tctcttgctt cgattgtagc gccagaattt cttttttcag gagtaaaagg aaaatcaaat atttccaatg cgtcatatca taaagatggc aactatatag taacggcgga cattcaaagc ttttacatta actgcaataa agaacacatt tttagattct ttaaatatac gcttcgcact tcagatgata ttgcgcgaat actaactgag ctttgctgct ataaaacttt cctgccaaca ggcagtccgg tcagccaaat cttagcctac cattcttacg caagaatatt caatagaatt gatgcttttt caaaagcgaa cgatatcact ttctccttat atgtagacga tattacgcta tcttcagaaa aatctattca tcgttatatc ctaaagacaa tatcaaaact tctaaagtcc gtaaacttat cattgaaaaa agaaaaaaca aaattttata ataaaaactc ctacaaaata gtaacaggat gcgcgattag tcctgatcac atactattac gacctaataa aattatgaga aagatcgata ataaactctg taaacatgaa aaggatctat cgaaattaac tccaaaagaa atcgaaagcg ttttaggcca agtgatatat ttaagatcta taaccccgaa ttcttacccc caattattca aatctttaag cttaatgaaa acagaagaaa aaattaaaca gagacccttg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat