SSGCID: Target Page

LpboA.19762.a

Uncharacterized protein

CENTER ID:	LpboA.19762.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19762.a.B1.GE42144	full length		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355277.4.peg.2721
UniProt:	Q04R54

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEIEVKNVLN ALNLFRNRIV EDCNTQLLNK NLIKEFHALI GKDLGENFAA IPGEFRKQNV TVGHVYKAPD YRDVESLIDS FCEWSKLEFH YEKGQTFSVA IIQEIVSHVY IAWIHPFGDG NGRTARLLEF YLLLRAGVPD IAAHILSNFY NSMRSEYYRK LDETSKNGGD LTHFINYALQ GFKDGLQEVF NIVNQNQVEL AWKNYVNETF KTNDFSGKSN IVNNRRLALV LSMNLENEYA FKEISEINEI LQLQYVGKSK RTLLRDIEAL LDMKLIVETK DEKYKTNVQI LTGRTAASVK GRDII

NT Sequence

atggaaattg aggttaaaaa cgtattgaat gcgcttaact tgtttcgaaa tagaatcgtt gaagattgta atactcaatt acttaataaa aatttaatta aggaatttca cgctttaatc ggtaaggatc ttggtgaaaa ttttgccgca attccaggcg agtttcgaaa acaaaacgtt acggttgggc acgtatataa ggcgccggac tacagggacg tagaaagtct gattgattcg ttttgtgaat ggagtaaatt agaatttcat tatgaaaaag gtcagacgtt ttcggtagca attattcaag agatcgtttc ccatgtgtac atagcttgga tacatccatt tggtgatggt aatggtcgaa cagcaagact actggaattc tatcttttgt tgcgagccgg agtgccggat attgccgcgc atattctgtc taatttttat aattcaatgc gaagcgaata ttataggaag ttggatgaaa cctcaaaaaa cggcggggac ttgacgcatt ttatcaacta tgcgttgcaa ggttttaaag atggtctaca agaagttttt aatatcgtga atcaaaatca agttgaatta gcttggaaaa attatgtgaa tgaaactttt aaaacgaatg atttttccgg aaaatcaaac atagtaaata atcgaagatt agctttggtt ctctctatga acttagagaa tgaatacgct tttaaggaaa tatctgaaat aaacgaaatt ttacaacttc aatacgtggg gaaatcaaaa cggaccttat taagggacat tgaagcttta cttgatatga aacttatcgt tgaaacaaaa gacgagaaat ataaaaccaa tgttcaaatt ctaacgggta gaacggcggc ttccgttaaa ggtcgggaca ttatttga

Details for LpboA.19762.a.B1.GE42144

HARVESTED ON:	1/16/2018
SEQUENCED ON:	1/17/2018
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME IEVKNVLNAL NLFRNRIVED CNTQLLNKNL IKEFHALIGK DLGENFAAIP GEFRKQNVTV GHVYKAPDYR DVESLIDSFC EWSKLEFHYE KGQTFSVAII QEIVSHVYIA WIHPFGDGNG RTARLLEFYL LLRAGVPDIA AHILSNFYNS MRSEYYRKLD ETSKNGGDLT HFINYALQGF KDGLQEVFNI VNQNQVELAW KNYVNETFKT NDFSGKSNIV NNRRLALVLS MNLENEYAFK EISEINEILQ LQYVGKSKRT LLRDIEALLD MKLIVETKDE KYKTNVQILT GRTAAXXXRS GHYLSE

Validated NT Sequence

atggctcacc accaccacca ccatatggaa attgaggtta aaaacgtatt gaatgcgctt aacttgtttc gaaatagaat cgttgaagat tgtaatactc aattacttaa taaaaattta attaaggaat ttcacgcttt aatcggtaag gatcttggtg aaaattttgc cgcaattcca ggcgagtttc gaaaacaaaa cgttacggtt gggcacgtat ataaggcgcc ggactacagg gacgtagaaa gtctgattga ttcgttttgt gaatggagta aattagaatt tcattatgaa aaaggtcaga cgttttcggt agcaattatt caagagatcg tttcccatgt gtacatagct tggatacatc catttggtga tggtaatggt cgaacagcaa gactactgga attctatctt ttgttgcgag ccggagtgcc ggatattgcc gcgcatattc tgtctaattt ttataattca atgcgaagcg aatattatag gaagttggat gaaacctcaa aaaacggcgg ggacttgacg cattttatca actatgcgtt gcaaggtttt aaagatggtc tacaagaagt ttttaatatc gtgaatcaaa atcaagttga attagcttgg aaaaattatg tgaatgaaac ttttaaaacg aatgattttt ccggaaaatc aaacatagta aataatcgaa gattagcttt ggttctctct atgaacttag agaatgaata cgcttttaag gaaatatctg aaataaacga aattttacaa cttcaatacg tggggaaatc aaaacggacc ttattaaggg acattgaagc tttacttgat atgaaactta tcgttgaaac aaaagacgag aaatataaaa ccaatgttca aattctaacg ggtagaacgg cggctnnnnn nnnaaggtcg ggacattatt taagtgagta a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa ttgaggttaa aaacgtattg aatgcgctta acttgtttcg aaatagaatc gttgaagatt gtaatactca attacttaat aaaaatttaa ttaaggaatt tcacgcttta atcggtaagg atcttggtga aaattttgcc gcaattccag gcgagtttcg aaaacaaaac gttacggttg ggcacgtata taaggcgccg gactacaggg acgtagaaag tctgattgat tcgttttgtg aatggagtaa attagaattt cattatgaaa aaggtcagac gttttcggta gcaattattc aagagatcgt ttcccatgtg tacatagctt ggatacatcc atttggtgat ggtaatggtc gaacagcaag actactggaa ttctatcttt tgttgcgagc cggagtgccg gatattgccg cgcatattct gtctaatttt tataattcaa tgcgaagcga atattatagg aagttggatg aaacctcaaa aaacggcggg gacttgacgc attttatcaa ctatgcgttg caaggtttta aagatggtct acaagaagtt tttaatatcg tgaatcaaaa tcaagttgaa ttagcttgga aaaattatgt gaatgaaact tttaaaacga atgatttttc cggaaaatca aacatagtaa ataatcgaag attagctttg gttctctcta tgaacttaga gaatgaatac gcttttaagg aaatatctga aataaacgaa attttacaac ttcaatacgt ggggaaatca aaacggacct tattaaggga cattgaagct ttacttgata tgaaacttat cgttgaaaca aaagacgaga aatataaaac caatgttcaa attctaacgg gtagaacggc ggcttccgtt aaaggtcggg acattatttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat