LpboA.19758.a

Uncharacterized protein

CENTER ID: LpboA.19758.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19758.a.B2.GE42143 mature protein 20 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.3472
UniProt: Q04PQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTKAGFTLL SSVFSPLNGG VNNSDTVNNV NVYKDAVRAQ LELQKQQAKN QWIADVNAYI QAELQIFLAT LSQNTSNSVT TNTNSVQTIN PTVQNVTTTP VSQNTNPAQA AQSYYQGSQL WNTKWQDLLT KQNTWEQNSL NAIQNGILQW NQSITGLEND KLSYLNGIEQ TKAQWLANKQ LITNTQSQMR NALQSTITNI RSQENQLKAN AASDPSLTSV FGDMDELLED LQDALNSNAS LGTLAQTLGN FFQSQISNAT AKADYWNITK WQETYATQVL EFKKEVGTAS LSCSGSSSCN NLSTIGPQAI TYGSDGSIYG WGATNGVLIS ALDPYTVMNP NYSELNQQYQ SCQTQASGSP IVYSQSKQYE RILQRRTLRS DV
NT Sequence
ttgtttacaa aggctggatt cacccttctg tcctccgtct tcagtcctct gaacggagga gtaaataact cggacacggt gaataacgtg aacgtttaca aagacgcggt aagagcacaa ctggaactgc aaaaacaaca ggcaaagaac caatggatcg cagacgtaaa cgcatacata caagcggaac tacaaatctt tttagcgaca ttatcccaaa acacatcgaa cagcgtaaca acaaacacaa attcggtaca aacaatcaat ccgaccgtgc aaaacgtaac cacaacaccc gtatcacaaa atacaaaccc cgcacaagcg gcacagagtt attaccaagg aagccaactc tggaatacca aatggcagga tctactaacc aaacaaaata cctgggaaca gaattcgttg aatgcgatcc agaatggtat tttacaatgg aatcaatcga ttacaggttt agaaaatgat aaattatcct atctgaatgg aatcgaacaa accaaagcgc agtggctggc aaacaaacaa ctgatcacaa acacccaaag ccaaatgaga aacgcgctac aatccacaat tacgaatata cgatcccaag aaaatcaatt gaaagcaaac gcagcgagtg atccgagttt gacgtccgtg ttcggcgaca tggacgagct gttggaggat ctacaagacg ctcttaactc caacgcatcc ctcggcacgt tagcgcaaac actgggaaac tttttccaaa gtcagatttc aaacgcaacc gcaaaagccg actactggaa tattaccaaa tggcaagaga cgtatgcgac tcaagtttta gaatttaaaa aagaagtggg aaccgcaagc ttaagttgta gtggaagtag ttcctgtaac aacctgtcta ccattggacc acaagcgatc acgtatggaa gcgacggaag catatatggt tggggtgcga ctaatggagt tttgataagc gctttggatc cgtatacggt aatgaatccg aattattcgg aactaaatca acaataccaa agttgccaga ctcaggcgtc cgggtctcca atcgtataca gtcaatccaa acaatacgag cggatactac agcgcaggac attacgaagc gacgtgtaa
Details for LpboA.19758.a.B2.GE42143
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG VNNSDTVNNV NVYKDAVRAQ LELQKQQAKN QWIADVNAYI QAELQIFLAT LSQNTSNSVT TNTNSVQTIN PTVQNVTTTP VSQNTNPAQA AQSYYQGSQL WNTKWQDLLT KQNTWEQNSL NAIQNGILQW NQSITGLEND KLSYLNGIEQ TKAQWLANKQ LITNTQSQMR NALQSTITNI RSQENQLKAN AASDPSLTSV FGDMDELLED LQDALNSNAS LGTLAQTLGN FFQSQISNAT AKADYWNITK WQETYATQVL EFKKEVGTAS LNCSGSSSCN NLSTIGPQAI TYGSDGSIYG WGATNGVLIS ALDPYTVMNP NYSELNQQYQ SCQTQASGSP IVYSQSKQYE RIL
Validated NT Sequence
atggctcacc accaccacca ccatatggga gtaaataact cggacacggt gaataacgtg aacgtttaca aagacgcggt aagagcacaa ctggaactgc aaaaacaaca ggcaaagaac caatggatcg cagacgtaaa cgcatacata caagcggaac tacaaatctt tttagcgaca ttatcccaaa acacatcgaa cagcgtaaca acaaacacaa attcggtaca aacaatcaat ccgaccgtgc aaaacgtaac cacaacaccc gtatcacaaa atacaaaccc cgcacaagcg gcacagagtt attaccaagg aagccaactc tggaatacca aatggcagga tctactaacc aaacaaaata cctgggaaca gaattcgttg aatgcgatcc agaatggtat tttacaatgg aatcaatcga ttacaggttt agaaaatgat aaattatcct atctgaatgg aatcgaacaa accaaagcgc agtggctggc aaacaaacaa ctgatcacaa acacccaaag ccaaatgaga aacgcgctac aatccacaat tacgaatata cgatcccaag aaaatcaatt gaaagcaaac gcagcgagtg atccgagttt gacgtccgtg ttcggcgaca tggacgagct gttggaggat ctacaagacg ctcttaactc caacgcatcc ctcggcacgt tagcgcaaac actgggaaac tttttccaaa gtcagatttc aaacgcaacc gcaaaagccg actactggaa tattaccaaa tggcaagaga cgtatgcgac tcaagtttta gaatttaaaa aagaagtggg aaccgcaagc ttaaattgta gtggaagtag ttcctgtaac aacctgtcta ccattggacc acaagcgatc acgtatggaa gcgacggaag catatatggt tggggtgcga ctaatggagt tttgataagc gctttggatc cgtatacggt aatgaatccg aattattcgg aactaaatca acaataccaa agttgccaga ctcaggcgtc cgggtctcca atcgtataca gtcaatccaa acaatacgag cggatactac a
Expected Protein Sequence
MAHHHHHHGV NNSDTVNNVN VYKDAVRAQL ELQKQQAKNQ WIADVNAYIQ AELQIFLATL SQNTSNSVTT NTNSVQTINP TVQNVTTTPV SQNTNPAQAA QSYYQGSQLW NTKWQDLLTK QNTWEQNSLN AIQNGILQWN QSITGLENDK LSYLNGIEQT KAQWLANKQL ITNTQSQMRN ALQSTITNIR SQENQLKANA ASDPSLTSVF GDMDELLEDL QDALNSNASL GTLAQTLGNF FQSQISNATA KADYWNITKW QETYATQVLE FKKEVGTASL SCSGSSSCNN LSTIGPQAIT YGSDGSIYGW GATNGVLISA LDPYTVMNPN YSELNQQYQS CQTQASGSPI VYSQSKQYER ILQRRTLRSD V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggagtaa ataactcgga cacggtgaat aacgtgaacg tttacaaaga cgcggtaaga gcacaactgg aactgcaaaa acaacaggca aagaaccaat ggatcgcaga cgtaaacgca tacatacaag cggaactaca aatcttttta gcgacattat cccaaaacac atcgaacagc gtaacaacaa acacaaattc ggtacaaaca atcaatccga ccgtgcaaaa cgtaaccaca acacccgtat cacaaaatac aaaccccgca caagcggcac agagttatta ccaaggaagc caactctgga ataccaaatg gcaggatcta ctaaccaaac aaaatacctg ggaacagaat tcgttgaatg cgatccagaa tggtatttta caatggaatc aatcgattac aggtttagaa aatgataaat tatcctatct gaatggaatc gaacaaacca aagcgcagtg gctggcaaac aaacaactga tcacaaacac ccaaagccaa atgagaaacg cgctacaatc cacaattacg aatatacgat cccaagaaaa tcaattgaaa gcaaacgcag cgagtgatcc gagtttgacg tccgtgttcg gcgacatgga cgagctgttg gaggatctac aagacgctct taactccaac gcatccctcg gcacgttagc gcaaacactg ggaaactttt tccaaagtca gatttcaaac gcaaccgcaa aagccgacta ctggaatatt accaaatggc aagagacgta tgcgactcaa gttttagaat ttaaaaaaga agtgggaacc gcaagcttaa gttgtagtgg aagtagttcc tgtaacaacc tgtctaccat tggaccacaa gcgatcacgt atggaagcga cggaagcata tatggttggg gtgcgactaa tggagttttg ataagcgctt tggatccgta tacggtaatg aatccgaatt attcggaact aaatcaacaa taccaaagtt gccagactca ggcgtccggg tctccaatcg tatacagtca atccaaacaa tacgagcgga tactacagcg caggacatta cgaagcgacg tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat