LpboA.19754.a

Methyltransferase

CENTER ID: LpboA.19754.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19754.a.B1.GE42140 full length 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.2314
UniProt: Q04SF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFLDDTVDVV DLIGKKRFTV YDTGLFNLQL ILNQKEKVNQ RFKEYSANLT FELNVYKQFF DDLDRKFLKE PGPVQEHLHQ MILEREGQTF MEFFESKVLE LQEQTKNFSK DENEAHGFFF RKQVWDFILH SAFMLRTNLK PRGYAGDYEM MKMIYENEIM GKSLFARLLH SYPLQIPAAN AVRNRRRMIA DQIREAVENH DNADFRAMSV ACGPADEIGE AFSDMVELKK IHFTLLDQDM EALRVAMNTV NQLELEKGIS IHVRYINDSV RSMLRIRDLP GVWGRFDFIY SMGLFDYLTP PVARAVLARL FEMLRPGGKL IVGNFHVSNP DKAFMEYWLD WVLYHRTEEE MLELASTLPG HSRIFFEKTG CQMFLETRAP NC
NT Sequence
ttgtttcttg acgataccgt agacgtggtc gatctgatcg gcaaaaagag attcaccgta tatgacaccg gactttttaa tttgcaattg atactcaatc aaaaagaaaa agtgaatcaa cgttttaaag aatatagcgc caacctcacg ttcgaattga acgtatataa acaattcttc gacgatttgg atcgaaaatt tttaaaagaa ccgggtccgg ttcaggaaca tttgcatcag atgattctgg agcgggaagg tcagacgttt atggagtttt tcgaatccaa agtactggaa ctccaggaac aaacaaaaaa tttctcaaag gacgaaaacg aagcgcacgg attttttttc agaaaacagg tctgggattt tattcttcat tccgctttta tgttgcggac gaatttaaaa cctcgcggat acgcgggaga ctacgaaatg atgaagatga tttatgaaaa tgaaatcatg ggtaaatctc ttttcgcaag gttgttgcac agttatcctc tacaaattcc ggccgcaaat gcggtacgca atcggcgtag gatgatcgcg gatcaaattc gggaggctgt agaaaatcac gacaacgcag attttcgggc tatgtccgtc gcttgcggtc ctgcggatga aatcggagag gctttcagcg atatggtcga gttgaaaaaa attcatttta ccttgcttga tcaggatatg gaagcgcttc gagttgcgat gaatacggtg aaccagctcg aacttgaaaa aggaatttcg attcacgtta gatacatcaa cgattccgta cgttccatgc ttcgtatccg agaccttccc ggagtttggg gaagattcga ctttatctat tcgatgggac ttttcgatta tctaactcct ccggttgcaa gggcggtact cgcgagactt ttcgagatgc ttaggcccgg cggtaagttg attgtaggaa actttcacgt cagtaatcct gataaagcgt ttatggagta ttggttggac tgggttctgt atcatagaac ggaagaagaa atgttagaat tggcttccac tcttccggga catagtcgca tctttttcga aaaaaccgga tgccaaatgt ttctggagac gcgggcgcct aactgttaa
Details for LpboA.19754.a.B1.GE42140
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMX XXDDTVDVVD LIGKKRFTVY DTGLFNLQLI LNQKEKVNQR FKEYSANLTF ELNVYKQFFD DLDRKFLKEP GPVQEHLHQM ILEREGQTFM EFFESKVLEL QEQTKNFSKD ENEAHGFFFR KQVWDFILHS AFMLRTNLKP RGYAGDYEMM KMIYENEIMG KSLFARLLHS YPLQIPAANA VRNRRRMIAD QIREAVENHD NADFRAMSVA CGPADEIGEA FSDMVELKKI HFTLLDQDME ALRVAMNTVN QLELEKGISI HVRYINDSVR SMLRIRDLPG VWGRFDFIYS MGLFDYLTPP VARAVLARLF EMLRPGGKLI VGNFHVSNPD KAFMEYWLDW VLYHRTEEEM LELASTLPGH SRIFFEKTGC
Validated NT Sequence
catggctcac caccaccacc accatatgtt nnnnnttgac gataccgtag acgtggtcga tctgatcggc aaaaagagat tcaccgtata tgacaccgga ctttttaatt tgcaattgat actcaatcaa aaagaaaaag tgaatcaacg ttttaaagaa tatagcgcca acctcacgtt cgaattgaac gtatataaac aattcttcga cgatttggat cgaaaatttt taaaagaacc gggtccggtt caggaacatt tgcatcagat gattctggag cgggaaggtc agacgtttat ggagtttttc gaatccaaag tactggaact ccaggaacaa acaaaaaatt tctcaaagga cgaaaacgaa gcgcacggat tttttttcag aaaacaggtc tgggatttta ttcttcattc cgcttttatg ttgcggacga atttaaaacc tcgcggatac gcgggagact acgaaatgat gaagatgatt tatgaaaatg aaatcatggg taaatctctt ttcgcaaggt tgttgcacag ttatcctcta caaattccgg ccgcaaatgc ggtacgcaat cggcgtagga tgatcgcgga tcaaattcgg gaggctgtag aaaatcacga caacgcagat tttcgggcta tgtccgtcgc ttgcggtcct gcggatgaaa tcggagaggc tttcagcgat atggtcgagt tgaaaaaaat tcattttacc ttgcttgatc aggatatgga agcgcttcga gttgcgatga atacggtgaa ccagctcgaa cttgaaaaag gaatttcgat tcacgttaga tacatcaacg attccgtacg ttccatgctt cgtatccgag accttcccgg agtttgggga agattcgact ttatctattc gatgggactt ttcgattatc taactcctcc ggttgcaagg gcggtactcg cgagactttt cgagatgctt aggcccggcg gtaagttgat tgtaggaaac tttcacgtca gtaatcctga taaagcgttt atggagtatt ggttggactg ggttctgtat catagaacgg aagaagaaat gttagaattg gcttccactc ttccgggaca tagtcgcatc tttttcgaaa aaaccggatg ccaa
Expected Protein Sequence
MAHHHHHHMF LDDTVDVVDL IGKKRFTVYD TGLFNLQLIL NQKEKVNQRF KEYSANLTFE LNVYKQFFDD LDRKFLKEPG PVQEHLHQMI LEREGQTFME FFESKVLELQ EQTKNFSKDE NEAHGFFFRK QVWDFILHSA FMLRTNLKPR GYAGDYEMMK MIYENEIMGK SLFARLLHSY PLQIPAANAV RNRRRMIADQ IREAVENHDN ADFRAMSVAC GPADEIGEAF SDMVELKKIH FTLLDQDMEA LRVAMNTVNQ LELEKGISIH VRYINDSVRS MLRIRDLPGV WGRFDFIYSM GLFDYLTPPV ARAVLARLFE MLRPGGKLIV GNFHVSNPDK AFMEYWLDWV LYHRTEEEML ELASTLPGHS RIFFEKTGCQ MFLETRAPNC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtttc ttgacgatac cgtagacgtg gtcgatctga tcggcaaaaa gagattcacc gtatatgaca ccggactttt taatttgcaa ttgatactca atcaaaaaga aaaagtgaat caacgtttta aagaatatag cgccaacctc acgttcgaat tgaacgtata taaacaattc ttcgacgatt tggatcgaaa atttttaaaa gaaccgggtc cggttcagga acatttgcat cagatgattc tggagcggga aggtcagacg tttatggagt ttttcgaatc caaagtactg gaactccagg aacaaacaaa aaatttctca aaggacgaaa acgaagcgca cggatttttt ttcagaaaac aggtctggga ttttattctt cattccgctt ttatgttgcg gacgaattta aaacctcgcg gatacgcggg agactacgaa atgatgaaga tgatttatga aaatgaaatc atgggtaaat ctcttttcgc aaggttgttg cacagttatc ctctacaaat tccggccgca aatgcggtac gcaatcggcg taggatgatc gcggatcaaa ttcgggaggc tgtagaaaat cacgacaacg cagattttcg ggctatgtcc gtcgcttgcg gtcctgcgga tgaaatcgga gaggctttca gcgatatggt cgagttgaaa aaaattcatt ttaccttgct tgatcaggat atggaagcgc ttcgagttgc gatgaatacg gtgaaccagc tcgaacttga aaaaggaatt tcgattcacg ttagatacat caacgattcc gtacgttcca tgcttcgtat ccgagacctt cccggagttt ggggaagatt cgactttatc tattcgatgg gacttttcga ttatctaact cctccggttg caagggcggt actcgcgaga cttttcgaga tgcttaggcc cggcggtaag ttgattgtag gaaactttca cgtcagtaat cctgataaag cgtttatgga gtattggttg gactgggttc tgtatcatag aacggaagaa gaaatgttag aattggcttc cactcttccg ggacatagtc gcatcttttt cgaaaaaacc ggatgccaaa tgtttctgga gacgcgggcg cctaactgtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat