LpboA.19749.a

Dehydrogenase

CENTER ID: LpboA.19749.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19749.a.B1.GE42137 full length 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.1456
UniProt: Q04TN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLRLRTALI GCGRIGAFTS LELKKSLPIG WLPYNHLDSI LLNKDLFDLI GFCDIKLANL EFLRKKFKDK QFYTDYRVLI DEQKPEVVSI ATRAQDRSEI IEYACKNGVK GLYIEKPAAR SLKELKIIKD SIIRNQVNFV YGVQRRFMDL YIKAKTKIDE GIIGEINEVH IGFGRSPLLW VHPHSVDMIL YFIGSTEIDY VQGSCEIAKE VVKKDFVDSD PILNFGFVRF KNGSTGVISS QTSINGMNAS IAGSEGIIHI FANGSKMEVQ LKKKIKNSNY SKLLSKDYFP STKTSGTYAA FRQLGKSIYS KDVSPYNILD LEIGHRMLFG FVQSSLRNGE RVRLNDVNEN LFISGRQGNL YP
NT Sequence
atgagtttaa gattaagaac tgcattaatc ggttgtggaa gaattggagc ttttacttcc cttgagttaa aaaaaagttt gcctatcggt tggttaccct ataatcattt agattctata ttattgaata aagatttatt tgatttaatt ggtttctgtg acattaaatt ggcaaaccta gagtttctta ggaaaaaatt taaagataaa caattttata ctgattatag ggttctaata gacgaacaaa aacccgaagt agttagtata gctacaagag ctcaagatag atcggaaatt atcgaatacg cttgcaaaaa tggtgtaaaa ggtttgtata ttgaaaagcc tgccgcacgt tctttaaaag aattaaagat aataaaagat tcgattataa gaaatcaggt gaattttgtt tatggtgtac agcgaagatt tatggattta tatataaaag cgaaaacaaa aatagatgaa ggtattatcg gtgaaattaa cgaagttcac ataggttttg gtagatctcc tttgttatgg gtgcatcctc attctgtaga tatgatttta tactttattg gttcgaccga gattgactat gttcaaggtt cttgtgagat tgcgaaagaa gtggtcaaaa aagatttcgt agactcagat ccaattttaa attttggatt cgtaagattt aaaaatgggt caaccggtgt tatttcttct caaacttcta ttaatggaat gaatgcttct attgctggat cagaaggaat tattcatata tttgcaaatg gcagtaagat ggaagtccaa ttaaaaaaga aaattaaaaa tagtaattat tcgaaacttt tatccaaaga ttattttcct tcaactaaaa caagtgggac ttatgctgct ttccgtcaat tgggaaaatc aatttattca aaggacgtct ccccctacaa tattcttgat ttggaaattg gtcatcgaat gttattcggc tttgtccaat ctagccttag aaatggagag cgtgttaggc taaatgatgt aaatgaaaat ttatttatta gcggaaggca aggaaatttg tatccatag
Details for LpboA.19749.a.B1.GE42137
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 68
Validated AA Sequence
MAHHHHHHMS LRLRTALIGC GRIGAFTSLE LKKSLPIGWL PYNHLDSILL NKDLFDLIGF CDIKLANLEF LMKKFKDKQF YTDYRVLIDE QKPEVVSIAT RAQDRSEIIE YACKNGVKGL YIEKPAARSL KELKIIKDSI IRNQVNFVYG VQRRFMDLYI KAKTKIDEGI IGEINEVHIG FGRSPLLWVH PHSVDMILYF IGSTEIDYVQ GSCEIAKEVV KKDFVDSDPI LNFGFVRFKN GSTGVISSQT FY
Validated NT Sequence
atggctcacc accaccacca ccatatgagt ttaagattaa gaactgcatt aatcggttgt ggaagaattg gagcttttac ttcccttgag ttaaaaaaaa gtttgcctat cggttggtta ccctataatc atttagattc tatattattg aataaagatt tatttgattt aattggtttc tgtgacatta aattggcaaa cctagagttt cttatgaaaa aatttaaaga taaacaattt tatactgatt atagggttct aatagacgaa caaaaacccg aagtagttag tatagctaca agagctcaag atagatcgga aattatcgaa tacgcttgca aaaatggtgt aaaaggtttg tatattgaaa agcctgccgc acgttcttta aaagaattaa agataataaa agattcgatt ataagaaatc aggtgaattt tgtttatggt gtacagcgaa gatttatgga tttatatata aaagcgaaaa caaaaataga tgaaggtatt atcggtgaaa ttaacgaagt tcacataggt tttggtagat ctcctttgtt atgggtgcat cctcattctg tagatatgat tttatacttt attggttcga ccgagattga ctatgttcaa ggttcttgtg agattgcgaa agaagtggtc aaaaaagatt tcgtagactc agatccaatt ttaaattttg gattcgtaag atttaaaaat gggtcaaccg gtgttatttc ttctcaaact ttctattaat ggaatgaatg ctttctattg ctggatnaga aggaattatt nnnatattnn caantggcag taagatggaa gtcnanntaa aaaaagaaaa ttaaaaaatt agtaatttat nngaaacnnn tatccaaagn nnnnnnnnnn ncnnnnaaaa caagtgg
Expected Protein Sequence
MAHHHHHHMS LRLRTALIGC GRIGAFTSLE LKKSLPIGWL PYNHLDSILL NKDLFDLIGF CDIKLANLEF LRKKFKDKQF YTDYRVLIDE QKPEVVSIAT RAQDRSEIIE YACKNGVKGL YIEKPAARSL KELKIIKDSI IRNQVNFVYG VQRRFMDLYI KAKTKIDEGI IGEINEVHIG FGRSPLLWVH PHSVDMILYF IGSTEIDYVQ GSCEIAKEVV KKDFVDSDPI LNFGFVRFKN GSTGVISSQT SINGMNASIA GSEGIIHIFA NGSKMEVQLK KKIKNSNYSK LLSKDYFPST KTSGTYAAFR QLGKSIYSKD VSPYNILDLE IGHRMLFGFV QSSLRNGERV RLNDVNENLF ISGRQGNLYP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt taagattaag aactgcatta atcggttgtg gaagaattgg agcttttact tcccttgagt taaaaaaaag tttgcctatc ggttggttac cctataatca tttagattct atattattga ataaagattt atttgattta attggtttct gtgacattaa attggcaaac ctagagtttc ttaggaaaaa atttaaagat aaacaatttt atactgatta tagggttcta atagacgaac aaaaacccga agtagttagt atagctacaa gagctcaaga tagatcggaa attatcgaat acgcttgcaa aaatggtgta aaaggtttgt atattgaaaa gcctgccgca cgttctttaa aagaattaaa gataataaaa gattcgatta taagaaatca ggtgaatttt gtttatggtg tacagcgaag atttatggat ttatatataa aagcgaaaac aaaaatagat gaaggtatta tcggtgaaat taacgaagtt cacataggtt ttggtagatc tcctttgtta tgggtgcatc ctcattctgt agatatgatt ttatacttta ttggttcgac cgagattgac tatgttcaag gttcttgtga gattgcgaaa gaagtggtca aaaaagattt cgtagactca gatccaattt taaattttgg attcgtaaga tttaaaaatg ggtcaaccgg tgttatttct tctcaaactt ctattaatgg aatgaatgct tctattgctg gatcagaagg aattattcat atatttgcaa atggcagtaa gatggaagtc caattaaaaa agaaaattaa aaatagtaat tattcgaaac ttttatccaa agattatttt ccttcaacta aaacaagtgg gacttatgct gctttccgtc aattgggaaa atcaatttat tcaaaggacg tctcccccta caatattctt gatttggaaa ttggtcatcg aatgttattc ggctttgtcc aatctagcct tagaaatgga gagcgtgtta ggctaaatga tgtaaatgaa aatttattta ttagcggaag gcaaggaaat ttgtatccat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat