SSGCID: Target Page

LpboA.19736.a

Uncharacterized protein

CENTER ID:	LpboA.19736.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19736.a.B1.GE42129	full length		1	223

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	Q04SF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDSNPDYVYA NDKKSKVNAP APYIALKLPV TESIDYDVAL YIPGGADQIT RNTPNGQSFN QWAGINIPGP IGNFKQIKET NSNQFAVVKL VNGISVRFGN LSLGASIEAI YGTQKLNQKY CDITGSLKTP RQGYYYESSK KAFSIGGVVG ANHTVTDRFR IGYSYQAHSS IPLNGSYQIG LNDPYYYRKS GVSHKFDLPE KHSLGFTYGP ENFKIAVDLI HTN

NT Sequence

ctggattcca atccggatta cgtttacgcc aacgataaaa agagcaaggt caacgccccc gctccttaca tcgctctcaa acttccggtc accgaatcca ttgattacga tgtcgctctt tatattcccg ggggtgcgga tcaaatcact cgaaatactc cgaacggcca gtccttcaat caatgggcgg gaatcaacat tccaggtccg atcggcaatt tcaaacaaat caaagaaact aattccaatc agtttgcagt cgtaaagctc gtgaacggaa tctcggttcg tttcggtaat ctttccttgg gtgcgagcat cgaagctata tacggaactc aaaagctcaa tcagaaatat tgcgatatca cgggctcttt aaaaactccc aggcaaggat actattacga aagcagtaaa aaagcatttt ccatcggcgg tgtcgtgggc gcgaaccata cagttaccga tcgttttaga atcggctatt catatcaggc ccattcttcc attcctctca acggaagtta tcaaatcggt ttgaacgacc cctactatta cagaaaatcc ggcgtttccc ataaattcga tcttcctgaa aaacattcat taggttttac ttatggtcct gaaaatttca aaatagcggt ggatctgatt catacgaatt ag

Details for LpboA.19736.a.B1.GE42129

HARVESTED ON:	1/16/2018
SEQUENCED ON:	1/17/2018
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML DSNPDYVYAN DKKSKVNAPA PYIALKLPVT ESIDYDVALY IPGGADQITR NTPNGQSFNQ WAGINIPGPI GNFKQIKETN SNQFAVVKLV NGISVRFGNL SLGASIEAIY GTQKLNQKYC DITGSLKTPR QGYYYESSKK AFSIGGVVGA NHTVTDRFRI GYSYQAHSSI PLNGSYQIGL NDPYYYRKSG VSHKFDLPEK HSLGFTYGPE NFKIAVDLIH TN

Validated NT Sequence

atggctcacc accaccacca ccatatgctg gattccaatc cggattacgt ttacgccaac gataaaaaga gcaaggtcaa cgcccccgct ccttacatcg ctctcaaact tccggtcacc gaatccattg attacgatgt cgctctttat attcccgggg gtgcggatca aatcactcga aatactccga acggccagtc cttcaatcaa tgggcgggaa tcaacattcc aggtccgatc ggcaatttca aacaaatcaa agaaactaat tccaatcagt ttgcagtcgt aaagctcgtg aacggaatct cggttcgttt cggtaatctt tccttgggtg cgagcatcga agctatatac ggaactcaaa agctcaatca gaaatattgc gatatcacgg gctctttaaa aactcccagg caaggatact attacgaaag cagtaaaaaa gcattttcca tcggcggtgt cgtgggcgcg aaccatacag ttaccgatcg ttttagaatc ggctattcat atcaggccca ttcttccatt cctctcaacg gaagttatca aatcggtttg aacgacccct actattacag aaaatccggc gtttcccata aattcgatct tcctgaaaaa cattcattag gttttactta tggtcctgaa aatttcaaaa tagcggtgga tctgattcat acgaattaag tgagtaagat

Expected Protein Sequence

MAHHHHHHMD SNPDYVYAND KKSKVNAPAP YIALKLPVTE SIDYDVALYI PGGADQITRN TPNGQSFNQW AGINIPGPIG NFKQIKETNS NQFAVVKLVN GISVRFGNLS LGASIEAIYG TQKLNQKYCD ITGSLKTPRQ GYYYESSKKA FSIGGVVGAN HTVTDRFRIG YSYQAHSSIP LNGSYQIGLN DPYYYRKSGV SHKFDLPEKH SLGFTYGPEN FKIAVDLIHT N

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctggatt ccaatccgga ttacgtttac gccaacgata aaaagagcaa ggtcaacgcc cccgctcctt acatcgctct caaacttccg gtcaccgaat ccattgatta cgatgtcgct ctttatattc ccgggggtgc ggatcaaatc actcgaaata ctccgaacgg ccagtccttc aatcaatggg cgggaatcaa cattccaggt ccgatcggca atttcaaaca aatcaaagaa actaattcca atcagtttgc agtcgtaaag ctcgtgaacg gaatctcggt tcgtttcggt aatctttcct tgggtgcgag catcgaagct atatacggaa ctcaaaagct caatcagaaa tattgcgata tcacgggctc tttaaaaact cccaggcaag gatactatta cgaaagcagt aaaaaagcat tttccatcgg cggtgtcgtg ggcgcgaacc atacagttac cgatcgtttt agaatcggct attcatatca ggcccattct tccattcctc tcaacggaag ttatcaaatc ggtttgaacg acccctacta ttacagaaaa tccggcgttt cccataaatt cgatcttcct gaaaaacatt cattaggttt tacttatggt cctgaaaatt tcaaaatagc ggtggatctg attcatacga attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat