SSGCID: Target Page

LpboA.19733.a

Uncharacterized protein

CENTER ID:	LpboA.19733.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19733.a.B1.GE42127	full length		1	454

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355277.4.peg.2817
UniProt:	Q04QY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKWKLTYEA LEFIGRDGAG WPIINLISDS YNVEDNSIVR VSVKAKNMVL KTLPENIVGV RVLDIERIDK MTGKNYFESY QIEDLNFELE ESDAKKLRKI QFDLLSIDGG SLDFDYLRLF NPDLFVMNLM NVKAFFVDGY GVLSHEEGVL VLENLNNFYN SFTSDQINVL RINPKSVSYD ENELIRKFLE QDRQVVDSSS KQNYVKNGSK VNLKFLEEKI KDSEIISDII DWIVENEENP ELLKNFSKLT PENLSKLNTL SGIVTLKNCL LRWEENLLHS SEDFWQKEFE DNSYVISQLF TSPVTIMKSK AYLGGKSIHN TGGSLMDYLF VNKLTKNTSI IELKTPATRL LGSNYRNTIY NISAELSGAI VQLSNYKDEL LKNYYPLTNL NKRIFHAYDP SCILIVGNFA TELDSEEKVK SFELFRQQLQ NIQIITFDEL FEKMRLLIHL LEGS

NT Sequence

atggcaaaat ggaaattaac ctatgaagcg ttggaattta taggtaggga cggggccgga tggcccatta ttaatctaat atctgattct tataatgttg aagataactc tatagtccgt gtctctgtaa aagcaaagaa tatggttttg aagaccttac cagagaacat tgttggagtt cgagttttgg atattgaaag aattgataaa atgacgggca aaaactactt tgaaagctat caaattgaag atcttaattt tgaattagaa gaaagtgatg ctaagaagtt acgaaaaata caatttgatc tattatccat tgacggcggt tcattagatt tcgattatct aagacttttt aatccagatt tgtttgttat gaatttaatg aatgtgaaag cattctttgt tgatggttat ggggtactaa gtcatgaaga aggagtttta gtattagaaa atttaaataa cttttataat tcatttactt ctgatcaaat caacgtttta agaattaatc ccaaaagtgt ctcttatgat gaaaacgaat taattcgtaa atttctcgaa caagatcgac aagtcgtaga ttcatctagt aaacaaaatt acgttaaaaa tggaagtaaa gttaatctaa aatttctaga agaaaaaatt aaagattcgg aaataatttc tgatataatt gattggatag ttgaaaacga ggaaaatcct gaattattaa aaaacttcag caaacttact ccggaaaatt taagcaaact aaatacatta tccggtattg taactttaaa gaattgttta ctacgatggg aggaaaatct actccattct tccgaagact tttggcaaaa agagtttgaa gataactcat acgtaatttc tcaacttttt acatctccag ttacaattat gaaatctaaa gcttacttag gtggcaagag tattcataat actggaggat cgttaatgga ttatcttttc gttaataaat taacaaagaa cacaagtata attgaattaa aaaccccggc cacaagatta ttaggctcga attatcgtaa taccatttat aatatttctg ctgaattatc tggagcgatt gtacagcttt ctaattataa agacgaactt ttaaaaaact attacccgct gacaaattta aataaaagaa tatttcatgc atatgatcct tcttgcatac tcatagtggg aaatttcgcg acggaattag attccgaaga aaaagtaaaa tctttcgagt tatttcgaca acaactacag aatattcaaa taattacttt cgatgaactt tttgaaaaaa tgcgactatt aatacattta ttagaaggta gttag

Details for LpboA.19733.a.B1.GE42127

HARVESTED ON:	1/16/2018
SEQUENCED ON:	1/17/2018
EXPECTED MW:	53kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA KWKLTYEALE FIGRDGAGWP IINLISDSYN VEDNSIVRVS VKAKNMVLKT LPENIVGVRV LDIERIDKMT GKNYFESYQI EDLNFELEES DAKKLRKIQF DLLSIDGGSL DFDYLRLFNP DLFVMNLMNV KAFFVDGYGV LSHEEGVLVL ENLNNFYNSF TSDQINVLRI NPKSVSYDEN ELIRKFLEQD RQVVDSSSKQ NYVKNGSKVN LKFLEEKIKD SEIISDIIDW IVENEENPEL LKNFSKLTPE NLSKLNTLSG IVTLKNCLLR WEENLLHSSE DFWQKEFEDN SYVISQLFTS PVTIMKSKAY LGGKSIHNTG GSLMDYLFVN KLTKNTSIIE LKTPATRLLG SNYRNTIYNI SAELSGAIVQ LSNYKDELLK NYYPLTNLNK RIFHAYDPSC ILIVGNFATE LDSEEKVKSF ELFRQQLQNI QIITFDELFE KMR

Validated NT Sequence

atggctcacc accaccacca ccatatggca aaatggaaat taacctatga agcgttggaa tttataggta gggacggggc cggatggccc attattaatc taatatctga ttcttataat gttgaagata actctatagt ccgtgtctct gtaaaagcaa agaatatggt tttgaagacc ttaccagaga acattgttgg agttcgagtt ttggatattg aaagaattga taaaatgacg ggcaaaaact actttgaaag ctatcaaatt gaagatctta attttgaatt agaagaaagt gatgctaaga agttacgaaa aatacaattt gatctattat ccattgacgg cggttcatta gatttcgatt atctaagact ttttaatcca gatttgtttg ttatgaattt aatgaatgtg aaagcattct ttgttgatgg ttatggggta ctaagtcatg aagaaggagt tttagtatta gaaaatttaa ataactttta taattcattt acttctgatc aaatcaacgt tttaagaatt aatcccaaaa gtgtctctta tgatgaaaac gaattaattc gtaaatttct cgaacaagat cgacaagtcg tagattcatc tagtaaacaa aattacgtta aaaatggaag taaagttaat ctaaaatttc tagaagaaaa aattaaagat tcggaaataa tttctgatat aattgattgg atagttgaaa acgaggaaaa tcctgaatta ttaaaaaact tcagcaaact tactccggaa aatttaagca aactaaatac attatccggt attgtaactt taaagaattg tttactacga tgggaggaaa atctactcca ttcttccgaa gacttttggc aaaaagagtt tgaagataac tcatacgtaa tttctcaact ttttacatct ccagttacaa ttatgaaatc taaagcttac ttaggtggca agagtattca taatactgga ggatcgttaa tggattatct tttcgttaat aaattaacaa agaacacaag tataattgaa ttaaaaaccc cggccacaag attattaggc tcgaattatc gtaataccat ttataatatt tctgctgaat tatctggagc gattgtacag ctttctaatt ataaagacga acttttaaaa aactattacc cgctgacaaa tttaaataaa agaatatttc atgcatatga tccttcttgc atactcatag tgggaaattt cgcgacggaa ttagattccg aagaaaaagt aaaatctttc gagttatttc gacaacaact acagaatatt caaataatta ctttcgatga actttttgaa aaaatgcgac t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa aatggaaatt aacctatgaa gcgttggaat ttataggtag ggacggggcc ggatggccca ttattaatct aatatctgat tcttataatg ttgaagataa ctctatagtc cgtgtctctg taaaagcaaa gaatatggtt ttgaagacct taccagagaa cattgttgga gttcgagttt tggatattga aagaattgat aaaatgacgg gcaaaaacta ctttgaaagc tatcaaattg aagatcttaa ttttgaatta gaagaaagtg atgctaagaa gttacgaaaa atacaatttg atctattatc cattgacggc ggttcattag atttcgatta tctaagactt tttaatccag atttgtttgt tatgaattta atgaatgtga aagcattctt tgttgatggt tatggggtac taagtcatga agaaggagtt ttagtattag aaaatttaaa taacttttat aattcattta cttctgatca aatcaacgtt ttaagaatta atcccaaaag tgtctcttat gatgaaaacg aattaattcg taaatttctc gaacaagatc gacaagtcgt agattcatct agtaaacaaa attacgttaa aaatggaagt aaagttaatc taaaatttct agaagaaaaa attaaagatt cggaaataat ttctgatata attgattgga tagttgaaaa cgaggaaaat cctgaattat taaaaaactt cagcaaactt actccggaaa atttaagcaa actaaataca ttatccggta ttgtaacttt aaagaattgt ttactacgat gggaggaaaa tctactccat tcttccgaag acttttggca aaaagagttt gaagataact catacgtaat ttctcaactt tttacatctc cagttacaat tatgaaatct aaagcttact taggtggcaa gagtattcat aatactggag gatcgttaat ggattatctt ttcgttaata aattaacaaa gaacacaagt ataattgaat taaaaacccc ggccacaaga ttattaggct cgaattatcg taataccatt tataatattt ctgctgaatt atctggagcg attgtacagc tttctaatta taaagacgaa cttttaaaaa actattaccc gctgacaaat ttaaataaaa gaatatttca tgcatatgat ccttcttgca tactcatagt gggaaatttc gcgacggaat tagattccga agaaaaagta aaatctttcg agttatttcg acaacaacta cagaatattc aaataattac tttcgatgaa ctttttgaaa aaatgcgact attaatacat ttattagaag gtagttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat