LpboA.19716.a

Uncharacterized protein

CENTER ID: LpboA.19716.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19716.a.B1.GE42123 full length 1 357
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.828
UniProt: Q04QB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRFIQFHFL TSYPPSNLNR DDLGRPKTAM LGGANRLRIS SQSLKRAWRT SDLFRETLSG HIGTRTKEKG KEIYQKLLDG KIPEKNARTW SKSIAGRFGK LKNPSEKELE KNPLLDLEIQ QLTHFSKEEN EAISSLIQKL IDSKKEPSNE DLNLLKKENS SSDIAMFGRM LASDPAFNIE AAVQVAHAIT VHKVAIEDDF FTAVDDLNSG IEDVGAGHLG EVEFGSGLFY LYICVNREQL LNNLNGNKDL AKRTIKALVE TSSTISPTGK QNSFASRVRA LYCMAEKGNE QPRSLHTAFL NSVEDRGILN AAILKLESSR QNFEKVYGSC ASGGFKTFNV DSGNGSLKEI LEFAQED
NT Sequence
atgaaccgtt tcattcaatt tcatttcctc acttcctacc ccccttccaa cttgaaccgg gacgatttag gaagacctaa aaccgcaatg ctgggagggg cgaataggct tcgtatttcc tctcaaagtt taaaaagggc ctggagaact tcggatcttt ttcgggaaac gttatccggc catatcggaa cgagaaccaa agagaaagga aaagaaatct atcagaaatt attggatgga aagattcccg aaaaaaacgc aagaacctgg agcaaatcga tcgcgggtag attcggaaaa cttaaaaatc cttcggaaaa agaattggaa aaaaatccgc ttttggatct ggagatccaa caactcactc atttcagcaa agaggagaac gaggcgattt cctccttaat ccaaaaactg atcgattcca aaaaagaacc ctccaacgag gatttgaatc ttttgaaaaa ggaaaattcc tcttcggata tcgccatgtt cggtagaatg ctcgcctccg accccgcgtt taacatcgaa gcggccgttc aagtcgccca cgcgattacg gttcacaagg ttgccatcga agacgatttt tttacggcag tcgacgactt aaacagcgga atcgaagacg taggagccgg tcatttagga gaagtggaat tcggttccgg tcttttttat ctttatatct gcgtcaatag agaacaactt cttaacaatc tgaacggtaa taaggatctc gccaaacgta cgataaaagc gttagtcgaa acatcatcta cgatttctcc cacgggaaaa caaaacagtt tcgcttccag agtgagagct ttgtactgta tggcggaaaa gggaaacgaa caacccagat ctctacatac cgcgtttcta aactccgtgg aagatagagg aattttgaat gcggcgattt tgaaattgga atccagtaga caaaacttcg aaaaagtata cggctcctgt gcttccgggg gttttaaaac tttcaacgtg gactccggca acggctcctt aaaagaaata ttagaatttg ctcaagagga ttag
Details for LpboA.19716.a.B1.GE42123
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/18/2018
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 20
Validated AA Sequence
MAEKGNEQPR SLHTAFLNSV EDRGILNAAI LKLESSRQNF EKVYGSCASG GFKTFNVDSG NGSLKEXXEF AQED
Validated NT Sequence
atcttactca cttaatcctc ttgagcaaat tcnnnnattt cttttaagga gccgttgccg gagtccacgt tgaaagtttt aaaacccccg gaagcacagg agccgtatac tttttcgaag ttttgtctac tggattccaa tttcaaaatc gccgcattca aaattcctct atcttccacg gagtttagaa acgcggtatg tagagatctg ggttgttcgt ttcccttttc cgccatacag tacaaagctc tcactctgga agcgaaactg ttttgttttc ccgtgggaga aatcgtagat gatgtttcga ctaacgcttt tatcgtacgt ttgncgagat ccttattanc nttcagattg ttannnnntt g
Expected Protein Sequence
MAHHHHHHMN RFIQFHFLTS YPPSNLNRDD LGRPKTAMLG GANRLRISSQ SLKRAWRTSD LFRETLSGHI GTRTKEKGKE IYQKLLDGKI PEKNARTWSK SIAGRFGKLK NPSEKELEKN PLLDLEIQQL THFSKEENEA ISSLIQKLID SKKEPSNEDL NLLKKENSSS DIAMFGRMLA SDPAFNIEAA VQVAHAITVH KVAIEDDFFT AVDDLNSGIE DVGAGHLGEV EFGSGLFYLY ICVNREQLLN NLNGNKDLAK RTIKALVETS STISPTGKQN SFASRVRALY CMAEKGNEQP RSLHTAFLNS VEDRGILNAA ILKLESSRQN FEKVYGSCAS GGFKTFNVDS GNGSLKEILE FAQED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc gtttcattca atttcatttc ctcacttcct accccccttc caacttgaac cgggacgatt taggaagacc taaaaccgca atgctgggag gggcgaatag gcttcgtatt tcctctcaaa gtttaaaaag ggcctggaga acttcggatc tttttcggga aacgttatcc ggccatatcg gaacgagaac caaagagaaa ggaaaagaaa tctatcagaa attattggat ggaaagattc ccgaaaaaaa cgcaagaacc tggagcaaat cgatcgcggg tagattcgga aaacttaaaa atccttcgga aaaagaattg gaaaaaaatc cgcttttgga tctggagatc caacaactca ctcatttcag caaagaggag aacgaggcga tttcctcctt aatccaaaaa ctgatcgatt ccaaaaaaga accctccaac gaggatttga atcttttgaa aaaggaaaat tcctcttcgg atatcgccat gttcggtaga atgctcgcct ccgaccccgc gtttaacatc gaagcggccg ttcaagtcgc ccacgcgatt acggttcaca aggttgccat cgaagacgat ttttttacgg cagtcgacga cttaaacagc ggaatcgaag acgtaggagc cggtcattta ggagaagtgg aattcggttc cggtcttttt tatctttata tctgcgtcaa tagagaacaa cttcttaaca atctgaacgg taataaggat ctcgccaaac gtacgataaa agcgttagtc gaaacatcat ctacgatttc tcccacggga aaacaaaaca gtttcgcttc cagagtgaga gctttgtact gtatggcgga aaagggaaac gaacaaccca gatctctaca taccgcgttt ctaaactccg tggaagatag aggaattttg aatgcggcga ttttgaaatt ggaatccagt agacaaaact tcgaaaaagt atacggctcc tgtgcttccg ggggttttaa aactttcaac gtggactccg gcaacggctc cttaaaagaa atattagaat ttgctcaaga ggattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat