LpboA.19714.a

Uncharacterized protein

CENTER ID: LpboA.19714.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19714.a.B1.GE42121 full length 1 221
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.3303
UniProt: Q04V60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPIRTIYAE CWLRSPAKLI DKSTCSKCGI GNKYEERPSP LFIEWENWGG ENKVIGDFTW PSGGRFIVKE TVFQKLNEIF ENLNSGPVEM IRSSKIKNTD SYQRPPLVEL MVDKQIPFLP ETTTKVAYRC PTCDRVQRDL IGVELKYERW NKTLEKSVKV HKKRKPGKGL ILSKFDIDLE VPFFRVTEFS DAIFCTDSIR ILLLSLGLNH LDFLDYGKIK K
NT Sequence
ttggaaccta tccgtacaat ttacgcggaa tgctggcttc gaagtcctgc caaactcatc gataagtcta cgtgtagtaa atgtggaatc ggaaataagt atgaggaaag accctctcca ctgttcatcg aatgggaaaa ttggggaggg gaaaacaaag taataggaga ttttacctgg ccatccggag gtcgattcat tgtgaaggaa accgtatttc aaaaattaaa cgaaattttt gaaaacctaa attccggtcc ggtagaaatg attcgatctt cgaaaattaa gaacactgat tcataccaaa gacctcctct cgtagaactc atggttgata agcaaattcc gttccttccg gaaacaacca caaaagtcgc atatcgatgc cctacctgcg atcgagttca aagagatcta attggagtag aactgaaata tgaacgctgg aataaaactt tggaaaaatc agtaaaagtg cataaaaaaa gaaaaccggg aaaagggctg attttatcga aattcgatat agatctcgaa gttccatttt ttcgtgtcac agagttcagc gacgcgatat tttgcactga ttcgattcgt attctattat tgtctctcgg cctgaatcat cttgattttc tggactatgg aaaaatcaaa aaatga
Details for LpboA.19714.a.B1.GE42121
HARVESTED ON: 1/16/2018
SEQUENCED ON: 1/17/2018
EXPECTED MW: 27kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML EPIRTIYAEC WLRSPAKLID KSTCSKCGIG NKYEERPSPL FIEWENWGGE NKVIGDFTWP SGGRFIVKET VFQKLNEIFE NLNSGPVEMI RSSKIKNTDS YQRPPLVELM VDKQIPFLPE TTTKVAYRCP TCDRVQRDLI GVELKYERWN KTLEKSVKVH KKRKPGKGLI LSKFDIDLEV PFFRVTEFSD AIFCTDSIRI LLLSLGLNHL DFLDYGKIKK
Validated NT Sequence
atggctcacc accaccacca ccatatgttg gaacctatcc gtacaattta cgcggaatgc tggcttcgaa gtcctgccaa actcatcgat aagtctacgt gtagtaaatg tggaatcgga aataagtatg aggaaagacc ctctccactg ttcatcgaat gggaaaattg gggaggggaa aacaaagtaa taggagattt tacctggcca tccggaggtc gattcattgt gaaggaaacc gtatttcaaa aattaaacga aatttttgaa aacctaaatt ccggtccggt agaaatgatt cgatcttcga aaattaagaa cactgattca taccaaagac ctcctctcgt agaactcatg gttgataagc aaattccgtt ccttccggaa acaaccacaa aagtcgcata tcgatgccct acctgcgatc gagttcaaag agatctaatt ggagtagaac tgaaatatga acgctggaat aaaactttgg aaaaatcagt aaaagtgcat aaaaaaagaa aaccgggaaa agggctgatt ttatcgaaat tcgatataga tctcgaagtt ccattttttc gtgtcacaga gttcagcgac gcgatatttt gcactgattc gattcgtatt ctattattgt ctctcggcct gaatcatctt gattttctgg actatggaaa aatcaaaaaa taagtgagta agat
Expected Protein Sequence
MAHHHHHHME PIRTIYAECW LRSPAKLIDK STCSKCGIGN KYEERPSPLF IEWENWGGEN KVIGDFTWPS GGRFIVKETV FQKLNEIFEN LNSGPVEMIR SSKIKNTDSY QRPPLVELMV DKQIPFLPET TTKVAYRCPT CDRVQRDLIG VELKYERWNK TLEKSVKVHK KRKPGKGLIL SKFDIDLEVP FFRVTEFSDA IFCTDSIRIL LLSLGLNHLD FLDYGKIKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggaac ctatccgtac aatttacgcg gaatgctggc ttcgaagtcc tgccaaactc atcgataagt ctacgtgtag taaatgtgga atcggaaata agtatgagga aagaccctct ccactgttca tcgaatggga aaattgggga ggggaaaaca aagtaatagg agattttacc tggccatccg gaggtcgatt cattgtgaag gaaaccgtat ttcaaaaatt aaacgaaatt tttgaaaacc taaattccgg tccggtagaa atgattcgat cttcgaaaat taagaacact gattcatacc aaagacctcc tctcgtagaa ctcatggttg ataagcaaat tccgttcctt ccggaaacaa ccacaaaagt cgcatatcga tgccctacct gcgatcgagt tcaaagagat ctaattggag tagaactgaa atatgaacgc tggaataaaa ctttggaaaa atcagtaaaa gtgcataaaa aaagaaaacc gggaaaaggg ctgattttat cgaaattcga tatagatctc gaagttccat tttttcgtgt cacagagttc agcgacgcga tattttgcac tgattcgatt cgtattctat tattgtctct cggcctgaat catcttgatt ttctggacta tggaaaaatc aaaaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t