LpboA.19704.a

Aminopeptidase

CENTER ID: LpboA.19704.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19704.a.B1.GE41947 full length 1 483
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19704.a.B1.PS38533 full length 1 483

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1522
UniProt: Q04TN0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSDSVLRYS FSYIDKIYNH IIALCEFDRL PGSHEYDLAL DYVISELKDQ NCELNVLNFP AKENYWNWTF PVGMSHWKDG RDDTKIKFYN DKNLKLLEIL IPGESEKEIF FITHLCHPKP SANDNASGPA MFIELIRYFA QNKPELSLRF LFTVEYWGTV AYFSKFLDVR KNCIAGISLD MVGGDQNLAG STMIVDEIPH HLTSNLDLFL YDHMQRLAHT GSYRMVGEPI LWARTQKVFY TGGSDHYILN DSTVAIPSTC LNTYPDRFYH KPEDTPDKIS KDTLNLFFST VIHVIADFAK SLNQDKERSI LLNYASIQKD LVRYLNEKIQ SFEKPHLKKD SFMICHFLNL FERKAEIQNS KERIQLFHLM DQLYLRTFGI SLQEKSFSEK PKFEKTYLGP LYRNQLFTII SGEERDRLLN FQSVDPLYFA KCELSINYLT LGYEINEISW LVDYHYKSNN TLLQGLTFFL DLLENYKYLK KLH
NT Sequence
atgagttcag attctgtatt gcgatactct ttttcttata ttgataagat ttataatcat attattgcat tatgtgaatt tgacaggtta cctggttcgc acgaatatga tttagcttta gattatgtga tatcagaatt aaaagaccaa aattgcgaac tgaatgttct aaattttccg gcaaaagaaa attactggaa ttggactttt cccgttggca tgtcgcattg gaaagacggt agagatgaca caaaaataaa attttataat gataaaaatt taaaattatt agaaatattg attccaggag agagcgaaaa ggaaattttt tttatcaccc atttatgcca tcctaaacct tctgccaatg ataatgcttc tgggccagcg atgtttattg aattgattcg gtattttgct caaaataaac cagaactttc gcttcgattt ctttttaccg ttgaatattg ggggaccgtt gcttattttt caaagttttt agatgtaaga aagaattgta ttgccggtat ttctttggat atggtcggag gagaccaaaa tttggcggga tccacgatga ttgtggatga aattccgcat cacctaacaa gtaacttaga tttattttta tatgatcata tgcaaaggct cgcacatact ggaagttata gaatggttgg ggaaccaata ctatgggcga gaactcaaaa agttttttat acgggtggaa gtgatcatta tattttaaac gattcgacgg tcgcgatccc atcgacatgt ttgaatactt atccggatcg tttttatcat aaaccggaag atactccgga caaaatttcg aaagatactt taaatttatt tttttctact gtgattcatg ttattgccga ttttgcgaag tctttaaatc aagacaaaga acgatccata cttttgaatt acgcttctat tcaaaaagat ttggttcgct atttgaatga aaaaattcaa tcttttgaaa agccacatct aaaaaaagat agttttatga tttgccattt tttgaatctt tttgaaagaa aagctgaaat tcaaaattcg aaagaaagaa ttcaactctt tcacttgatg gatcaattgt atttgagaac ctttgggatt tcgcttcaag aaaaatcatt cagtgagaaa cccaaatttg aaaaaacgta tttagggcca ctttatcgaa atcaattatt taccattatt tctggcgagg aaagagatcg acttttaaat tttcaatccg tcgatccgtt atactttgct aaatgtgaat tatctataaa ttatctcact ttaggttatg aaataaatga aatttcatgg ttagtggatt accattataa aagtaataat actttattac aggggttaac ttttttcttg gatcttttgg aaaattacaa atatttaaag aagctgcatt ga
Details for LpboA.19704.a.B1.GE41947
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 58kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS SDSVLRYSFS YIDKIYNHII ALCEFDRLPG SHEYDLALDY VISELKDQNC ELNVLNFPAK ENYWNWTFPV GMSHWKDGRD DTKIKFYNDK NLKLLEILIP GESEKEIFFI THLCHPKPSA NDNASGPAMF IELIRYFAQN KPELSLRFLF TVEYWGTVAY FSKFLDVRKN CIAGISLDMV GGDQNLAGST MIVDEIPHHL TSNLDLFLYD HMQRLAHTGS YRMVGEPILW ARTQKVFYTG GSDHYILNDS TVAIPSTCLN TYPDRFYHKP EDTPDKISKD TLNLFFSTVI HVIADFAKSL NQDKERSILL NYASIQKDLV RYLNEKIQSF EKPHLKKDSF MICHFLNLFE RKAEIQNSKE RIQLFHLMDQ LYLRTFGISL QEKSFSEKPK FEKTYLGPLY RNQLFTIISG EERDRLLNFQ SVDPLYFAKC ELSINYLTLG YEINEISWLV DYHYKSNNTL LQGLTFFLDL LE
Validated NT Sequence
tttccaaaag atccaagaaa aaagttaacc cctgtaataa agtattatta cttttataat ggtaatccac taaccatgaa atttcattta tttcataacc taaagtgaga taatttatag ataattcaca tttagcaaag tataacggat cgacggattg aaaatttaaa agtcgatctc tttcctcgcc agaaataatg gtaaataatt gatttcgata aagtggccct aaatacgttt tttcaaattt gggtttctca ctgaatgatt tttcttgaag cgaaatccca aaggttctca aatacaattg atccatcaag tgaaagagtt gaattctttc tttcgaattt tgaatttcag cttttctttc aaaaagattc aaaaaatggc aaatcataaa actatctttt tttagatgtg gcttttcaaa agattgaatt ttttcattca aatagcgaac caaatctttt tgaatagaag cgtaattcaa aagtatggat cgttctttgt cttgatttaa agacttcgca aaatcggcaa taacatgaat cacagtagaa aaaaataaat ttaaagtatc tttcgaaatt ttgtccggag tatcttccgg tttatgataa aaacgatccg gataagtatt caaacatgtc gatgggatcg cgaccgtcga atcgtttaaa atataatgat cacttccacc cgtataaaaa actttttgag ttctcgccca tagtattggt tccccaacca ttctataact tccagtatgt gcgagccttt gcatatgatc atataaaaat aaatctaagt tacttgttag gtgatgcgga atttcatcca caatcatcgt ggatcccgcc aaattttggt ctcctccgac catatccaaa gaaataccgg caatacaatt ctttcttaca tctaaaaact ttgaaaaata agcaacggtc ccccaatatt caacggtaaa aagaaatcga agcgaaagtt ctggtttatt ttgagcaaaa taccgaatca attcaataaa catcgctggc ccagaagcat tatcattggc agaaggttta ggatggcata aatgggtgat aaaaaaaatt tccttttcgc tctctcctgg aatcaatatt tctaataatt ttaaattttt atcattataa aattttattt ttgtgtcatc tctaccgtct ttccaatgcg acatgccaac gggaaaagtc caattccagt aattttcttt tgccggaaaa tttagaacat tcagttcgca attttggtct tttaattctg atatcacata atctaaagct aaatcatatt cgtgcgaacc aggtaacctg tcaaattcac ataatgcaat aatatgatta taaatcttat caatataaga aaaagagtat cgcaatacag aatctgaact catatggtgg tggtggtggt gagccatggt at
Expected Protein Sequence
MAHHHHHHMS SDSVLRYSFS YIDKIYNHII ALCEFDRLPG SHEYDLALDY VISELKDQNC ELNVLNFPAK ENYWNWTFPV GMSHWKDGRD DTKIKFYNDK NLKLLEILIP GESEKEIFFI THLCHPKPSA NDNASGPAMF IELIRYFAQN KPELSLRFLF TVEYWGTVAY FSKFLDVRKN CIAGISLDMV GGDQNLAGST MIVDEIPHHL TSNLDLFLYD HMQRLAHTGS YRMVGEPILW ARTQKVFYTG GSDHYILNDS TVAIPSTCLN TYPDRFYHKP EDTPDKISKD TLNLFFSTVI HVIADFAKSL NQDKERSILL NYASIQKDLV RYLNEKIQSF EKPHLKKDSF MICHFLNLFE RKAEIQNSKE RIQLFHLMDQ LYLRTFGISL QEKSFSEKPK FEKTYLGPLY RNQLFTIISG EERDRLLNFQ SVDPLYFAKC ELSINYLTLG YEINEISWLV DYHYKSNNTL LQGLTFFLDL LENYKYLKKL H
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt cagattctgt attgcgatac tctttttctt atattgataa gatttataat catattattg cattatgtga atttgacagg ttacctggtt cgcacgaata tgatttagct ttagattatg tgatatcaga attaaaagac caaaattgcg aactgaatgt tctaaatttt ccggcaaaag aaaattactg gaattggact tttcccgttg gcatgtcgca ttggaaagac ggtagagatg acacaaaaat aaaattttat aatgataaaa atttaaaatt attagaaata ttgattccag gagagagcga aaaggaaatt ttttttatca cccatttatg ccatcctaaa ccttctgcca atgataatgc ttctgggcca gcgatgttta ttgaattgat tcggtatttt gctcaaaata aaccagaact ttcgcttcga tttcttttta ccgttgaata ttgggggacc gttgcttatt tttcaaagtt tttagatgta agaaagaatt gtattgccgg tatttctttg gatatggtcg gaggagacca aaatttggcg ggatccacga tgattgtgga tgaaattccg catcacctaa caagtaactt agatttattt ttatatgatc atatgcaaag gctcgcacat actggaagtt atagaatggt tggggaacca atactatggg cgagaactca aaaagttttt tatacgggtg gaagtgatca ttatatttta aacgattcga cggtcgcgat cccatcgaca tgtttgaata cttatccgga tcgtttttat cataaaccgg aagatactcc ggacaaaatt tcgaaagata ctttaaattt atttttttct actgtgattc atgttattgc cgattttgcg aagtctttaa atcaagacaa agaacgatcc atacttttga attacgcttc tattcaaaaa gatttggttc gctatttgaa tgaaaaaatt caatcttttg aaaagccaca tctaaaaaaa gatagtttta tgatttgcca ttttttgaat ctttttgaaa gaaaagctga aattcaaaat tcgaaagaaa gaattcaact ctttcacttg atggatcaat tgtatttgag aacctttggg atttcgcttc aagaaaaatc attcagtgag aaacccaaat ttgaaaaaac gtatttaggg ccactttatc gaaatcaatt atttaccatt atttctggcg aggaaagaga tcgactttta aattttcaat ccgtcgatcc gttatacttt gctaaatgtg aattatctat aaattatctc actttaggtt atgaaataaa tgaaatttca tggttagtgg attaccatta taaaagtaat aatactttat tacaggggtt aacttttttc ttggatcttt tggaaaatta caaatattta aagaagctgc attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for LpboA.19704.a.B1.PS38533
PURIFICATION DATe: 11/20/2018
CONCENTRATION: 4.62mg/ml
OBSERVED MW: 50kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS SDSVLRYSFS YIDKIYNHII ALCEFDRLPG SHEYDLALDY VISELKDQNC ELNVLNFPAK ENYWNWTFPV GMSHWKDGRD DTKIKFYNDK NLKLLEILIP GESEKEIFFI THLCHPKPSA NDNASGPAMF IELIRYFAQN KPELSLRFLF TVEYWGTVAY FSKFLDVRKN CIAGISLDMV GGDQNLAGST MIVDEIPHHL TSNLDLFLYD HMQRLAHTGS YRMVGEPILW ARTQKVFYTG GSDHYILNDS TVAIPSTCLN TYPDRFYHKP EDTPDKISKD TLNLFFSTVI HVIADFAKSL NQDKERSILL NYASIQKDLV RYLNEKIQSF EKPHLKKDSF MICHFLNLFE RKAEIQNSKE RIQLFHLMDQ LYLRTFGISL QEKSFSEKPK FEKTYLGPLY RNQLFTIISG EERDRLLNFQ SVDPLYFAKC ELSINYLTLG YEINEISWLV DYHYKSNNTL LQGLTFFLDL LE
Validated NT Sequence
tttccaaaag atccaagaaa aaagttaacc cctgtaataa agtattatta cttttataat ggtaatccac taaccatgaa atttcattta tttcataacc taaagtgaga taatttatag ataattcaca tttagcaaag tataacggat cgacggattg aaaatttaaa agtcgatctc tttcctcgcc agaaataatg gtaaataatt gatttcgata aagtggccct aaatacgttt tttcaaattt gggtttctca ctgaatgatt tttcttgaag cgaaatccca aaggttctca aatacaattg atccatcaag tgaaagagtt gaattctttc tttcgaattt tgaatttcag cttttctttc aaaaagattc aaaaaatggc aaatcataaa actatctttt tttagatgtg gcttttcaaa agattgaatt ttttcattca aatagcgaac caaatctttt tgaatagaag cgtaattcaa aagtatggat cgttctttgt cttgatttaa agacttcgca aaatcggcaa taacatgaat cacagtagaa aaaaataaat ttaaagtatc tttcgaaatt ttgtccggag tatcttccgg tttatgataa aaacgatccg gataagtatt caaacatgtc gatgggatcg cgaccgtcga atcgtttaaa atataatgat cacttccacc cgtataaaaa actttttgag ttctcgccca tagtattggt tccccaacca ttctataact tccagtatgt gcgagccttt gcatatgatc atataaaaat aaatctaagt tacttgttag gtgatgcgga atttcatcca caatcatcgt ggatcccgcc aaattttggt ctcctccgac catatccaaa gaaataccgg caatacaatt ctttcttaca tctaaaaact ttgaaaaata agcaacggtc ccccaatatt caacggtaaa aagaaatcga agcgaaagtt ctggtttatt ttgagcaaaa taccgaatca attcaataaa catcgctggc ccagaagcat tatcattggc agaaggttta ggatggcata aatgggtgat aaaaaaaatt tccttttcgc tctctcctgg aatcaatatt tctaataatt ttaaattttt atcattataa aattttattt ttgtgtcatc tctaccgtct ttccaatgcg acatgccaac gggaaaagtc caattccagt aattttcttt tgccggaaaa tttagaacat tcagttcgca attttggtct tttaattctg atatcacata atctaaagct aaatcatatt cgtgcgaacc aggtaacctg tcaaattcac ataatgcaat aatatgatta taaatcttat caatataaga aaaagagtat cgcaatacag aatctgaact catatggtgg tggtggtggt gagccatggt at
Expressed Protein Sequence
MAHHHHHHMS SDSVLRYSFS YIDKIYNHII ALCEFDRLPG SHEYDLALDY VISELKDQNC ELNVLNFPAK ENYWNWTFPV GMSHWKDGRD DTKIKFYNDK NLKLLEILIP GESEKEIFFI THLCHPKPSA NDNASGPAMF IELIRYFAQN KPELSLRFLF TVEYWGTVAY FSKFLDVRKN CIAGISLDMV GGDQNLAGST MIVDEIPHHL TSNLDLFLYD HMQRLAHTGS YRMVGEPILW ARTQKVFYTG GSDHYILNDS TVAIPSTCLN TYPDRFYHKP EDTPDKISKD TLNLFFSTVI HVIADFAKSL NQDKERSILL NYASIQKDLV RYLNEKIQSF EKPHLKKDSF MICHFLNLFE RKAEIQNSKE RIQLFHLMDQ LYLRTFGISL QEKSFSEKPK FEKTYLGPLY RNQLFTIISG EERDRLLNFQ SVDPLYFAKC ELSINYLTLG YEINEISWLV DYHYKSNNTL LQGLTFFLDL LENYKYLKKL H
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTT CAGATTCTGT ATTGCGATAC TCTTTTTCTT ATATTGATAA GATTTATAAT CATATTATTG CATTATGTGA ATTTGACAGG TTACCTGGTT CGCACGAATA TGATTTAGCT TTAGATTATG TGATATCAGA ATTAAAAGAC CAAAATTGCG AACTGAATGT TCTAAATTTT CCGGCAAAAG AAAATTACTG GAATTGGACT TTTCCCGTTG GCATGTCGCA TTGGAAAGAC GGTAGAGATG ACACAAAAAT AAAATTTTAT AATGATAAAA ATTTAAAATT ATTAGAAATA TTGATTCCAG GAGAGAGCGA AAAGGAAATT TTTTTTATCA CCCATTTATG CCATCCTAAA CCTTCTGCCA ATGATAATGC TTCTGGGCCA GCGATGTTTA TTGAATTGAT TCGGTATTTT GCTCAAAATA AACCAGAACT TTCGCTTCGA TTTCTTTTTA CCGTTGAATA TTGGGGGACC GTTGCTTATT TTTCAAAGTT TTTAGATGTA AGAAAGAATT GTATTGCCGG TATTTCTTTG GATATGGTCG GAGGAGACCA AAATTTGGCG GGATCCACGA TGATTGTGGA TGAAATTCCG CATCACCTAA CAAGTAACTT AGATTTATTT TTATATGATC ATATGCAAAG GCTCGCACAT ACTGGAAGTT ATAGAATGGT TGGGGAACCA ATACTATGGG CGAGAACTCA AAAAGTTTTT TATACGGGTG GAAGTGATCA TTATATTTTA AACGATTCGA CGGTCGCGAT CCCATCGACA TGTTTGAATA CTTATCCGGA TCGTTTTTAT CATAAACCGG AAGATACTCC GGACAAAATT TCGAAAGATA CTTTAAATTT ATTTTTTTCT ACTGTGATTC ATGTTATTGC CGATTTTGCG AAGTCTTTAA ATCAAGACAA AGAACGATCC ATACTTTTGA ATTACGCTTC TATTCAAAAA GATTTGGTTC GCTATTTGAA TGAAAAAATT CAATCTTTTG AAAAGCCACA TCTAAAAAAA GATAGTTTTA TGATTTGCCA TTTTTTGAAT CTTTTTGAAA GAAAAGCTGA AATTCAAAAT TCGAAAGAAA GAATTCAACT CTTTCACTTG ATGGATCAAT TGTATTTGAG AACCTTTGGG ATTTCGCTTC AAGAAAAATC ATTCAGTGAG AAACCCAAAT TTGAAAAAAC GTATTTAGGG CCACTTTATC GAAATCAATT ATTTACCATT ATTTCTGGCG AGGAAAGAGA TCGACTTTTA AATTTTCAAT CCGTCGATCC GTTATACTTT GCTAAATGTG AATTATCTAT AAATTATCTC ACTTTAGGTT ATGAAATAAA TGAAATTTCA TGGTTAGTGG ATTACCATTA TAAAAGTAAT AATACTTTAT TACAGGGGTT AACTTTTTTC TTGGATCTTT TGGAAAATTA CAAATATTTA AAGAAGCTGC ATTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT