SSGCID: Target Page

LpboA.19701.a

Uncharacterized protein

CENTER ID:	LpboA.19701.a
ORGANISM:	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpboA.19701.a.B1.GE41946	full length		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|355276.3.peg.1610
UniProt:	Q04TF7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGHERLGILP KTRSWINITS NLGVAIGENP EFASKLAGLT LLQVRQRYEK LKNDSGVQAA FSFLIAICSQ NLPSNAEGKF TNVDLNLESN PSPLNIAFGL TEWVEKHKDK QEYAELAARA AADAIAKWYR ENSEQNLLFE SGLTATDVWG NFSGSDFCVI SRNFFANLTE RYLKYFLERS ASELSPDLNS RKKFEQALSS FMNEVSNHAF ETSKITQSFA AGWYNKNVKN SRPADKEVAG FLKVAFGKLR EEIIIEEAKK

NT Sequence

atgggacacg aacgattagg aattttaccc aaaactagaa gttggatcaa tattacttct aatcttggtg tagctatagg cgagaatcct gaatttgctt caaaattagc tgggctaact ttattacaag ttagacagcg atatgaaaaa cttaaaaatg attcaggggt tcaagcagct ttctcatttt taattgctat ttgctctcaa aatttaccat caaacgctga agggaagttt acaaacgttg atcttaatct tgagtctaat ccatctccac ttaatattgc ctttgggtta actgaatggg ttgagaaaca taaagataag caagaatacg ctgaacttgc tgcgagagcc gcagcggatg ccatagctaa atggtataga gaaaattctg aacagaatct tctttttgaa tcaggtttaa cagctacaga tgtatggggc aatttttcag ggagtgactt ttgtgttatt tcgagaaatt ttttcgcgaa tttaactgaa agatatttga aatacttctt ggaaagaagt gcatctgaat tgtctccaga cttaaatagc agaaagaagt ttgaacaggc gctctcttct tttatgaatg aagtttcgaa tcacgctttt gaaacttcta aaattactca gtcattcgca gcaggatggt ataataaaaa tgtaaaaaac tctaggcctg ctgataaaga agtagcgggt tttttaaaag tcgcatttgg taaattacga gaagagataa tcattgaaga ggcgaaaaag tga

Details for LpboA.19701.a.B1.GE41946

HARVESTED ON:	10/3/2017
SEQUENCED ON:	10/12/2017
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG HERLGILPKT RSWINITSNL GVAIGENPEF ASKLAGLTLL QVRQRYEKLK NDSGVQAAFS FLIAICSQNL PSNAEGKFTN VDLNLESNPS PLNIAFGLTE WVEKHKDKQE YAELAARAAA DAIAKWYREN SEQNLLFESG LTATDVWGNF SGSDFCVISR NFFANLTERY LKYFLERSAS ELSPDLNSRK KFEQALSSFM NEVSNHAFET SKITQSFAAG WYNKNVKNSR PADKEVAGFL KVAFGKLREE IIIEEAKK

Validated NT Sequence

atcttactca cttacttttt cgcctcttca atgattatct cttctcgtaa tttaccaaat gcgactttta aaaaacccgc tacttcttta tcagcaggcc tagagttttt tacattttta ttataccatc ctgctgcgaa tgactgagta attttagaag tttcaaaagc gtgattcgaa acttcattca taaaagaaga gagcgcctgt tcaaacttct ttctgctatt taagtctgga gacaattcag atgcacttct ttccaagaag tatttcaaat atctttcagt taaattcgcg aaaaaatttc tcgaaataac acaaaagtca ctccctgaaa aattgcccca tacatctgta gctgttaaac ctgattcaaa aagaagattc tgttcagaat tttctctata ccatttagct atggcatccg ctgcggctct cgcagcaagt tcagcgtatt cttgcttatc tttatgtttc tcaacccatt cagttaaccc aaaggcaata ttaagtggag atggattaga ctcaagatta agatcaacgt ttgtaaactt cccttcagcg tttgatggta aattttgaga gcaaatagca attaaaaatg agaaagctgc ttgaacccct gaatcatttt taagtttttc atatcgctgt ctaacttgta ataaagttag cccagctaat tttgaagcaa attcaggatt ctcgcctata gctacaccaa gattagaagt aatattgatc caacttctag ttttgggtaa aattcctaat cgttcgtgtc ccatatggtg gtggtggtgg tgagccatgg tata

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggac acgaacgatt aggaatttta cccaaaacta gaagttggat caatattact tctaatcttg gtgtagctat aggcgagaat cctgaatttg cttcaaaatt agctgggcta actttattac aagttagaca gcgatatgaa aaacttaaaa atgattcagg ggttcaagca gctttctcat ttttaattgc tatttgctct caaaatttac catcaaacgc tgaagggaag tttacaaacg ttgatcttaa tcttgagtct aatccatctc cacttaatat tgcctttggg ttaactgaat gggttgagaa acataaagat aagcaagaat acgctgaact tgctgcgaga gccgcagcgg atgccatagc taaatggtat agagaaaatt ctgaacagaa tcttcttttt gaatcaggtt taacagctac agatgtatgg ggcaattttt cagggagtga cttttgtgtt atttcgagaa attttttcgc gaatttaact gaaagatatt tgaaatactt cttggaaaga agtgcatctg aattgtctcc agacttaaat agcagaaaga agtttgaaca ggcgctctct tcttttatga atgaagtttc gaatcacgct tttgaaactt ctaaaattac tcagtcattc gcagcaggat ggtataataa aaatgtaaaa aactctaggc ctgctgataa agaagtagcg ggttttttaa aagtcgcatt tggtaaatta cgagaagaga taatcattga agaggcgaaa aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat