LpboA.19694.a

Uncharacterized protein

CENTER ID: LpboA.19694.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19694.a.B1.GE41942 full length 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1667
UniProt: Q04RY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFPELRRIQ DFSVVHLNDS IRDTSLLQAD VNLFILDSPK GNVIYSNSNQ SIRYFHDDRI ENYKQIYVLP NGKILFISSD NFLDLCYTYS ATGNITFTPN CTKTNIGAYS IFEFKPDNGE VILLDHQKRL KKFNITDNSL TALNINPQPV EIFSLAYIAY NNGFLGILEN KGTKLTLFSE GNGRFNFTNS LEDFSFVTQK GIRYNSPQFL GIAVNSSGVI YTTCPSEDAI YSFNEDLKIT DVYEFLPEIR PYKKIINPHK ILISKRNEYL YVSNRSALEI LKDSANNPSE DFQWILPVQS DSIENAFKNL VNSGGYDPNK STFDQPGIKE IVQSWKGNL
NT Sequence
ttggatttcc ccgaattacg tagaattcag gatttttcgg tggttcattt aaacgattct attcgtgata cgagtctttt acaggccgat gtgaatcttt tcattctaga ttcgccaaaa ggaaacgtta tttattccaa ttcaaatcaa agtattcggt attttcatga cgatcgtatc gaaaactata aacaaattta cgttcttccc aacggaaaaa tcctcttcat ttcctccgat aactttttag atctgtgtta cacatacagt gcaactggta acattacctt cactccaaac tgtaccaaaa cgaacatagg cgcctattca atttttgaat ttaagccgga taacggagaa gtgattttat tggatcacca gaagcgttta aaaaaattca atataaccga caattcttta accgctttga acataaatcc gcaaccggta gaaatttttt ctttagccta tatagcctat aataacggtt tcctaggtat tttagaaaac aaagggacca agttaacttt attctccgaa ggaaacggac gattcaattt tacaaactcc ttagaagact tttccttcgt tacacaaaaa ggaatcaggt acaactcccc tcagttttta ggcatcgcag tcaactcaag cggcgtcatt tacacaactt gcccttccga agatgcgatc tactcattca acgaagattt aaaaatcacc gacgtttacg aatttctccc cgagataagg ccttataaaa aaattatcaa tccccataag atcttaatct caaaaaggaa cgaatacctt tatgtttcca atcgatccgc cttagaaatt ttgaaagact ctgcaaataa tccaagcgaa gactttcaat ggattttacc ggttcaatcg gattccattg aaaacgcatt taagaattta gtgaactcag gcggatatga tccaaacaaa agtacctttg atcagcccgg cataaaagaa atcgttcaat cttggaaggg gaatttgtga
Details for LpboA.19694.a.B1.GE41942
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML DFPELRRIQD FSVVHLNDSI RDTSLLQADV NLFILDSPKG NVIYSNSNQS IRYFHDDRIE NYKQIYVLPN GKILFISSDN FLDLCYTYSA TGNITFTPNC TKTNIGAYSI FEFKPDNGEV ILLDHQKRLK KFNITDNSLT ALNINPQPVE IFSLAYIAYN NGFLGILENK GTKLTLFSEG NGRFNFTNSL EDFSFVTQKG IRYNSPQFLG IAVNSSGVIY TTCPSEDAIY SFNEDLKITD VYEFLPEIRP YKKIINPHKI LISKRNEYLY VSNRSALEIL KDSANNPSED FQWILPVQSD SIENAFKNLV NSGGYDPNKS TFDQPGIK
Validated NT Sequence
ttcttttatg ccgggctgat caaaggtact tttgtttgga tcatatccgc ctgagttcac taaattctta aatgcgtttt caatggaatc cgattgaacc ggtaaaatcc attgaaagtc ttcgcttgga ttatttgcag agtctttcaa aatttctaag gcggatcgat tggaaacata aaggtattcg ttcctttttg agattaagat cttatgggga ttgataattt ttttataagg ccttatctcg gggagaaatt cgtaaacgtc ggtgattttt aaatcttcgt tgaatgagta gatcgcatct tcggaagggc aagttgtgta aatgacgccg cttgagttga ctgcgatgcc taaaaactga ggggagttgt acctgattcc tttttgtgta acgaaggaaa agtcttctaa ggagtttgta aaattgaatc gtccgtttcc ttcggagaat aaagttaact tggtcccttt gttttctaaa atacctagga aaccgttatt ataggctata taggctaaag aaaaaatttc taccggttgc ggatttatgt tcaaagcggt taaagaattg tcggttatat tgaatttttt taaacgcttc tggtgatcca ataaaatcac ttctccgtta tccggcttaa attcaaaaat tgaataggcg cctatgttcg ttttggtaca gtttggagtg aaggtaatgt taccagttgc actgtatgtg taacacagat ctaaaaagtt atcggaggaa atgaagagga tttttccgtt gggaagaacg taaatttgtt tatagttttc gatacgatcg tcatgaaaat accgaatact ttgatttgaa ttggaataaa taacgtttcc ttttggcgaa tctagaatga aaagattcac atcggcctgt aaaagactcg tatcacgaat agaatcgttt aaatgaacca ccgaaaaatc ctgaattcta cgtaattcgg ggaaatccaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMD FPELRRIQDF SVVHLNDSIR DTSLLQADVN LFILDSPKGN VIYSNSNQSI RYFHDDRIEN YKQIYVLPNG KILFISSDNF LDLCYTYSAT GNITFTPNCT KTNIGAYSIF EFKPDNGEVI LLDHQKRLKK FNITDNSLTA LNINPQPVEI FSLAYIAYNN GFLGILENKG TKLTLFSEGN GRFNFTNSLE DFSFVTQKGI RYNSPQFLGI AVNSSGVIYT TCPSEDAIYS FNEDLKITDV YEFLPEIRPY KKIINPHKIL ISKRNEYLYV SNRSALEILK DSANNPSEDF QWILPVQSDS IENAFKNLVN SGGYDPNKST FDQPGIKEIV QSWKGNL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggatt tccccgaatt acgtagaatt caggattttt cggtggttca tttaaacgat tctattcgtg atacgagtct tttacaggcc gatgtgaatc ttttcattct agattcgcca aaaggaaacg ttatttattc caattcaaat caaagtattc ggtattttca tgacgatcgt atcgaaaact ataaacaaat ttacgttctt cccaacggaa aaatcctctt catttcctcc gataactttt tagatctgtg ttacacatac agtgcaactg gtaacattac cttcactcca aactgtacca aaacgaacat aggcgcctat tcaatttttg aatttaagcc ggataacgga gaagtgattt tattggatca ccagaagcgt ttaaaaaaat tcaatataac cgacaattct ttaaccgctt tgaacataaa tccgcaaccg gtagaaattt tttctttagc ctatatagcc tataataacg gtttcctagg tattttagaa aacaaaggga ccaagttaac tttattctcc gaaggaaacg gacgattcaa ttttacaaac tccttagaag acttttcctt cgttacacaa aaaggaatca ggtacaactc ccctcagttt ttaggcatcg cagtcaactc aagcggcgtc atttacacaa cttgcccttc cgaagatgcg atctactcat tcaacgaaga tttaaaaatc accgacgttt acgaatttct ccccgagata aggccttata aaaaaattat caatccccat aagatcttaa tctcaaaaag gaacgaatac ctttatgttt ccaatcgatc cgccttagaa attttgaaag actctgcaaa taatccaagc gaagactttc aatggatttt accggttcaa tcggattcca ttgaaaacgc atttaagaat ttagtgaact caggcggata tgatccaaac aaaagtacct ttgatcagcc cggcataaaa gaaatcgttc aatcttggaa ggggaatttg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat