LpboA.19693.a

PP-loop superfamily protein

CENTER ID: LpboA.19693.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19693.a.B1.GE41941 full length 1 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.1551
UniProt: Q04TK4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQNSKEKIRV CKNCVMDTTD PNITFNEKGV CNHCLNFFQN IKPEWDKKLS NPSLLQEMIE KIKLAGKGKD YDCILGISGG VDSSYLAYLA KEKFGLRPLL FHVDAGWNSQ EAVNNIEKIV DGLKLDLITE VVNWEEMKDL QLSFFRAGVP HLDTPQDHVF FASLYNYCAK HGFHYILNGG NYSTECIREP LEWHYHASDL RQLKDIHRKF GKRKLKTFPL AGIFKYKIYY RIFKRLKVVQ PLNYVPYTKE EAIRELETNF GWQRYSHKHY ESRFTKFYEG YWLPNKFGYD KRKAHYSSLV LTNQKTREDA LKEIGQKPYD KLSISKDFDY IASKLDITRE ELERLEKLEN KSYRDYKSTS GLISLGTKIF RMLGIEKRII R
NT Sequence
atgcaaaatt caaaagagaa gattcgagtg tgtaagaatt gcgtaatgga cactaccgat ccaaatataa cattcaatga aaagggtgtt tgtaatcatt gtttgaactt ttttcaaaat atcaaacccg aatgggataa aaaactgagt aatccctctt tacttcagga gatgatagaa aagatcaaat tggccggaaa gggcaaggac tacgattgta ttcttggaat cagtggtggg gtggatagtt cttacttggc ctatttagcg aaggagaaat tcggtctaag acctcttttg tttcatgtag acgcgggttg gaattcgcaa gaagcggtca ataatatcga gaagatagtc gacggattga aattagacct aatcactgaa gtggtaaatt gggaagagat gaaagactta cagttgtctt tttttagagc gggagttcct cacttggata ctccccaaga ccacgttttt ttcgcctcgc tttataatta ttgtgcaaaa cacggatttc attatatttt aaacggcggg aactattcca ccgaatgtat acgagaaccc ttggaatggc actatcacgc ttcggattta agacagttga aggacattca tagaaaattt ggaaaaagaa aattaaaaac ctttcctttg gcggggatat tcaaatataa aatatattat agaatattca aaagattgaa agttgttcaa cctctgaatt acgttcctta tacgaaagag gaagccattc gagaacttga aaccaacttc ggttggcaga gatacagtca taaacactac gaatctagat tcacaaaatt ttacgaagga tattggctgc cgaataaatt cggatacgat aaaagaaaag cccattattc aagtctggtt ttgaccaatc aaaagacgag agaggacgcg ctgaaggaaa tcggtcaaaa gccgtatgat aaactttcca tctcgaagga tttcgactac atcgcttcca aattggatat tacaagagag gaactggaga gattggaaaa gttggaaaat aaatcctata gagattacaa atccacaagc ggtttgatca gtcttggaac gaaaattttt agaatgcttg ggatcgaaaa aaggatcatt cggtga
Details for LpboA.19693.a.B1.GE41941
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MQNSKEKIRV CKNCVMDTTD PNITFNEKGV CNHCLNFFQN IKPEWDKKLS NPSLLQEMIE KIKLAGKGKD YDCILGISGG VDSSYLAYLA KEKFGLRPLL FHVDAGWNSQ EAVNNIEKIV DGLKLDLITE VVNWEEMKDL QLSFFRAGVP HLDTPQDHVF FASLYNYCAK HGFHYILNGG NYSTECIREP LEWHYHASDL RQLKDIHRKF GKRKLKTFPL AGIFKYKIYY RIFKRLKVVQ PLNYVPYTKE EAIRELETNF GWQRYSHKHY ESRFTKFYEG YWLPNKFGYD KRKAHYSSLV LTNQKTREDA LKEIGQKPYD KLSISKDFDY IASKLDITRE ELERLEKLEN KSYRDYKSTS GLISLGTKIF R
Validated NT Sequence
cattctaaaa attttcgttc caagactgat caaaccgctt gtggatttgt aatctctata ggatttattt tccaactttt ccaatctctc cagttcctct cttgtaatat ccaatttgga agcgatgtag tcgaaatcct tcgagatgga aagtttatca tacggctttt gaccgatttc cttcagcgcg tcctctctcg tcttttgatt ggtcaaaacc agacttgaat aatgggcttt tcttttatcg tatccgaatt tattcggcag ccaatatcct tcgtaaaatt ttgtgaatct agattcgtag tgtttatgac tgtatctctg ccaaccgaag ttggtttcaa gttctcgaat ggcttcctct ttcgtataag gaacgtaatt cagaggttga acaactttca atcttttgaa tattctataa tatattttat atttgaatat ccccgccaaa ggaaaggttt ttaattttct ttttccaaat tttctatgaa tgtccttcaa ctgtcttaaa tccgaagcgt gatagtgcca ttccaagggt tctcgtatac attcggtgga atagttcccg ccgtttaaaa tataatgaaa tccgtgtttt gcacaataat tataaagcga ggcgaaaaaa acgtggtctt ggggagtatc caagtgagga actcccgctc taaaaaaaga caactgtaag tctttcatct cttcccaatt taccacttca gtgattaggt ctaatttcaa tccgtcgact atcttctcga tattattgac cgcttcttgc gaattccaac ccgcgtctac atgaaacaaa agaggtctta gaccgaattt ctccttcgct aaataggcca agtaagaact atccacccca ccactgattc caagaataca atcgtagtcc ttgccctttc cggccaattt gatcttttct atcatctcct gaagtaaaga gggattactc agttttttat cccattcggg tttgatattt tgaaaaaagt tcaaacaatg attacaaaca cccttttcat tgaatgttat atttggatcg gtagtgtcca ttacgcaatt cttacacact cgaatcttct cttttgaatt ttgcatatgg tggtggtggt ggtg
Expected Protein Sequence
MAHHHHHHMQ NSKEKIRVCK NCVMDTTDPN ITFNEKGVCN HCLNFFQNIK PEWDKKLSNP SLLQEMIEKI KLAGKGKDYD CILGISGGVD SSYLAYLAKE KFGLRPLLFH VDAGWNSQEA VNNIEKIVDG LKLDLITEVV NWEEMKDLQL SFFRAGVPHL DTPQDHVFFA SLYNYCAKHG FHYILNGGNY STECIREPLE WHYHASDLRQ LKDIHRKFGK RKLKTFPLAG IFKYKIYYRI FKRLKVVQPL NYVPYTKEEA IRELETNFGW QRYSHKHYES RFTKFYEGYW LPNKFGYDKR KAHYSSLVLT NQKTREDALK EIGQKPYDKL SISKDFDYIA SKLDITREEL ERLEKLENKS YRDYKSTSGL ISLGTKIFRM LGIEKRIIR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa attcaaaaga gaagattcga gtgtgtaaga attgcgtaat ggacactacc gatccaaata taacattcaa tgaaaagggt gtttgtaatc attgtttgaa cttttttcaa aatatcaaac ccgaatggga taaaaaactg agtaatccct ctttacttca ggagatgata gaaaagatca aattggccgg aaagggcaag gactacgatt gtattcttgg aatcagtggt ggggtggata gttcttactt ggcctattta gcgaaggaga aattcggtct aagacctctt ttgtttcatg tagacgcggg ttggaattcg caagaagcgg tcaataatat cgagaagata gtcgacggat tgaaattaga cctaatcact gaagtggtaa attgggaaga gatgaaagac ttacagttgt ctttttttag agcgggagtt cctcacttgg atactcccca agaccacgtt tttttcgcct cgctttataa ttattgtgca aaacacggat ttcattatat tttaaacggc gggaactatt ccaccgaatg tatacgagaa cccttggaat ggcactatca cgcttcggat ttaagacagt tgaaggacat tcatagaaaa tttggaaaaa gaaaattaaa aacctttcct ttggcgggga tattcaaata taaaatatat tatagaatat tcaaaagatt gaaagttgtt caacctctga attacgttcc ttatacgaaa gaggaagcca ttcgagaact tgaaaccaac ttcggttggc agagatacag tcataaacac tacgaatcta gattcacaaa attttacgaa ggatattggc tgccgaataa attcggatac gataaaagaa aagcccatta ttcaagtctg gttttgacca atcaaaagac gagagaggac gcgctgaagg aaatcggtca aaagccgtat gataaacttt ccatctcgaa ggatttcgac tacatcgctt ccaaattgga tattacaaga gaggaactgg agagattgga aaagttggaa aataaatcct atagagatta caaatccaca agcggtttga tcagtcttgg aacgaaaatt tttagaatgc ttgggatcga aaaaaggatc attcggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t