LpboA.19668.b

Signal transduction protein

CENTER ID: LpboA.19668.b
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19668.b.B2.GE41927 soluble domain 393 708
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355277.4.peg.4008
UniProt: Q04NL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFELRKSKT IFFLILAWSG AFFAIHSEPS AEFEPNAVMK GWNLQPYLTY YEDKSGELSF SEIQSIFRSG KSIPLFNTNL GYSDATIWVR IPVSNSEKRT KNWVLLFSYC LIDSLQFYSV RNGLSPLVVS GDKLPFATRL AENRNFPFQL SEPPLSKNDY YIRIESKNSL IIPLSAYSRT QFLEQTAKEY TVLGLYYGTM FVMFIYNLFL LITIRDISYL YYSIFIFFDI LFQMTLNGLS FQFLWPNNPR WGNVSLPFFM FSALLAACLF GKSFLESSKM TPITNKFYYP IIAICGFGCV GSLTFFTYTF SVISSIIVLL IVLTLLLTNS LQCVWKGHRP ARFFLMAWSV LIFGSFLYAM SAFGVFSDNF FTQWGLQIGS AIEVALLSLG LADRIKQLSL TLEMQMTSLG LLKQRHEQSA VQYKNLYEGE EDFLFDLDFN GTITGSNKSI STFLGFKSQD VIGKNFFDLV YKTGSLEDVF KKLYVMEKME ELYSTRKPVY FRVDFVQKYL KEPKELQVRL QVLEHKYGRD ILGRAFEPDR DLIGRFLDEE RIVFSMDNSL QNAELLSQRL TFNLIRFTNP AVVLSIRTGL REILVNSIEH GNLNISDEDK TRSLKSGNYF QFILTRQNDS HYRDKKILVE YFLSSQKVGY RITDEGKGFN HARIVRNALS KTNENTTLRT RGIAFALSTF DVVKFNSTGN RVTLVKYF
NT Sequence
gtgattttcg agcttcggaa atctaagacc attttctttt tgattttggc ttggtccgga gcttttttcg cgattcattc cgaaccgtcc gccgaattcg aaccgaatgc ggtaatgaaa ggatggaatc tgcaaccgta tctaacgtat tacgaagaca aatcggggga actttctttt tccgagattc aaagtatctt tcggtctggg aaatcgattc cgctgttcaa tactaacctc ggatactctg atgcaacgat ttgggttcgg attccggttt ctaattcgga aaagagaaca aaaaattggg ttcttctatt ctcttattgt ctgatcgact ctttgcagtt ttattccgtt cgaaacgggc tttcacctct ggtggtttcc ggagataaac ttccgtttgc aacgagactt gcagaaaatc ggaatttccc atttcaattg agcgaacctc ctctttccaa aaatgactat tatattcgaa tcgaatccaa aaattcgctt attattccct tatccgcgta ttcacggacc cagtttttgg agcaaaccgc gaaagaatat accgtcctag gtttgtacta cggaacgatg tttgtgatgt ttatttacaa tctgtttctt ttgattacga ttcgagatat aagttatctt tattatagta tattcatatt ttttgatatt ctgttccaaa tgactctgaa cggtttgtcc tttcagtttt tatggcctaa taatccaaga tgggggaacg taagtcttcc gttttttatg ttttccgcgc ttcttgcggc gtgtttgttt ggaaaatcct ttttggaatc ttctaaaatg actccgatca cgaacaaatt ttattatcca atcatagcga tttgtgggtt cggttgtgtc ggttctttga cttttttcac ttatacgttc agcgtgattt caagtattat cgttcttttg attgttttaa ctttgctttt gaccaacagt cttcaatgtg tatggaaagg acatcgtcca gctcgatttt tcctaatggc ttggtccgtt ttgatttttg ggagtttttt gtacgcgatg agtgcctttg gagtgttttc agataacttt tttactcaat ggggcttgca gattggatcc gcgattgaag tcgctcttct ttctttggga ttggcagacc ggatcaaaca actttcctta actttagaaa tgcaaatgac tagtttaggc cttttaaagc aaagacacga acaatctgca gttcagtata aaaatttata cgaaggcgag gaagattttc ttttcgattt ggatttcaac ggaacgatta caggaagtaa taagtctatc tctacgtttt taggttttaa atcgcaggat gtgatcggta aaaatttttt tgatctagtg tataaaacag ggagtttgga ggacgtattc aagaaattgt atgtaatgga aaagatggaa gagttgtatt ccacgaggaa accagtttat ttccgagtcg atttcgttca gaaatatctg aaggagccga aagagttaca ggttcgcctt caagttttgg agcacaaata tggtcgcgat atcttaggaa gagcttttga accggatcgg gatctgatag ggagatttct agatgaggaa agaatcgtgt tctcaatgga caattctctt caaaacgcgg aacttctgag tcagagactt acgtttaatc ttatacgttt tactaatcct gctgtggttc tttcgattcg gacgggcctt agagaaatac tcgtcaactc gattgagcac ggaaatctta atatatccga cgaagataaa accagatcct taaaaagtgg aaattatttt caatttatcc taactcgtca gaatgattca cattacaggg ataaaaaaat tctcgtggaa tattttctct ccagtcaaaa ggtgggttat agaattacgg atgaagggaa aggattcaat cacgcgagaa ttgttcggaa tgctctttct aaaacgaatg aaaatacgac tttacggact agaggaatcg cgtttgcact ttccactttc gacgtagtga aattcaattc tacaggaaac cgagtcacct tggttaagta tttctaa
Details for LpboA.19668.b.B2.GE41927
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 38kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMD RIKQLSLTLE MQMTSLGLLK QRHEQSAVQY KNLYEGEEDF LFDLDFNGTI TGSNKSISTF LGFKSQDVIG KNFFDLVYKT GSLEDVFKKL YVMEKMEELY STRKPVYFRV DFVQKYLKEP KELQVRLQVL EHKYGRDILG RAFEPDRDLI GRFLDEERIV FSMDNSLQNA ELLSQRLTFN LIRFTNPAVV LSIRTGLREI LVNSIEHGNL NISDEDKTRS LKSGNYFQFI LTRQNDSHYR DKKILVEYFL SSQKVGYRIT DEGKGFNHAR IVRNALSKTN ENTTLRTRGI AFALSTFDVV KFNSTG
Validated NT Sequence
ttcctgtaga attgaatttc actacgtcga aagtggaaag tgcaaacgcg attcctctag tccgtaaagt cgtattttca ttcgttttag aaagagcatt ccgaacaatt ctcgcgtgat tgaatccttt cccttcatcc gtaattctat aacccacctt ttgactggag agaaaatatt ccacgagaat ttttttatcc ctgtaatgtg aatcattctg acgagttagg ataaattgaa aataatttcc actttttaag gatctggttt tatcttcgtc ggatatatta agatttccgt gctcaatcga gttgacgagt atttctctaa ggcccgtccg aatcgaaaga accacagcag gattagtaaa acgtataaga ttaaacgtaa gtctctgact cagaagttcc gcgttttgaa gagaattgtc cattgagaac acgattcttt cctcatctag aaatctccct atcagatccc gatccggttc aaaagctctt cctaagatat cgcgaccata tttgtgctcc aaaacttgaa ggcgaacctg taactctttc ggctccttca gatatttctg aacgaaatcg actcggaaat aaactggttt cctcgtggaa tacaactctt ccatcttttc cattacatac aatttcttga atacgtcctc caaactccct gttttataca ctagatcaaa aaaattttta ccgatcacat cctgcgattt aaaacctaaa aacgtagaga tagacttatt acttcctgta atcgttccgt tgaaatccaa atcgaaaaga aaatcttcct cgccttcgta taaattttta tactgaactg cagattgttc gtgtctttgc tttaaaaggc ctaaactagt catttgcatt tctaaagtta aggaaagttg tttgatccgg tccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHDR IKQLSLTLEM QMTSLGLLKQ RHEQSAVQYK NLYEGEEDFL FDLDFNGTIT GSNKSISTFL GFKSQDVIGK NFFDLVYKTG SLEDVFKKLY VMEKMEELYS TRKPVYFRVD FVQKYLKEPK ELQVRLQVLE HKYGRDILGR AFEPDRDLIG RFLDEERIVF SMDNSLQNAE LLSQRLTFNL IRFTNPAVVL SIRTGLREIL VNSIEHGNLN ISDEDKTRSL KSGNYFQFIL TRQNDSHYRD KKILVEYFLS SQKVGYRITD EGKGFNHARI VRNALSKTNE NTTLRTRGIA FALSTFDVVK FNSTGNRVTL VKYF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaccgga tcaaacaact ttccttaact ttagaaatgc aaatgactag tttaggcctt ttaaagcaaa gacacgaaca atctgcagtt cagtataaaa atttatacga aggcgaggaa gattttcttt tcgatttgga tttcaacgga acgattacag gaagtaataa gtctatctct acgtttttag gttttaaatc gcaggatgtg atcggtaaaa atttttttga tctagtgtat aaaacaggga gtttggagga cgtattcaag aaattgtatg taatggaaaa gatggaagag ttgtattcca cgaggaaacc agtttatttc cgagtcgatt tcgttcagaa atatctgaag gagccgaaag agttacaggt tcgccttcaa gttttggagc acaaatatgg tcgcgatatc ttaggaagag cttttgaacc ggatcgggat ctgataggga gatttctaga tgaggaaaga atcgtgttct caatggacaa ttctcttcaa aacgcggaac ttctgagtca gagacttacg tttaatctta tacgttttac taatcctgct gtggttcttt cgattcggac gggccttaga gaaatactcg tcaactcgat tgagcacgga aatcttaata tatccgacga agataaaacc agatccttaa aaagtggaaa ttattttcaa tttatcctaa ctcgtcagaa tgattcacat tacagggata aaaaaattct cgtggaatat tttctctcca gtcaaaaggt gggttataga attacggatg aagggaaagg attcaatcac gcgagaattg ttcggaatgc tctttctaaa acgaatgaaa atacgacttt acggactaga ggaatcgcgt ttgcactttc cactttcgac gtagtgaaat tcaattctac aggaaaccga gtcaccttgg ttaagtattt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat