LpboA.19658.a

Hypothetical lipoprotein

CENTER ID: LpboA.19658.a
ORGANISM: Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpboA.19658.a.B2.GE41888 mature protein 23 268
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|355276.3.peg.2906
UniProt: Q04UE7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKLIRKILT VVLVLAMVGC SRHYYVKEFP VSGKAKVEKA PKIAYLGFRT YQSRVTGSAS RRTTYTAELV YETRTIPKLE NGVFINQLKS SGFRGDIPSD KVQAFAMEYL GAVKSSGALE ISTLVDVEKK GGDVKIFKLR NFPVDYYVIG VHGPAFRKNT NFGISVVEVF SSLFSMVTLG LIPVYSSDLA KTEVKIYDKN LKLVNSLEYD NSYSTIDAIW ASPNPPHCKM LECTEQIGSP PSIVYSEMGP RIEEDVLNSI QKPAAPTN
NT Sequence
atggataagc tgataaggaa aattttaacg gttgtgttgg ttcttgcgat ggtcggttgc agcaggcatt attacgtgaa ggagtttcct gtttccggga aagctaaagt ggaaaaggct cctaaaattg catatctcgg ttttagaacg tatcaatcca gagtaaccgg gtctgcgagc agaaggacta cgtatacagc cgagttagtc tatgaaacta gaaccattcc gaagttggaa aacggtgttt ttattaatca gctgaaaagc agcgggttta gaggtgatat tccttccgat aaggttcaag cttttgcaat ggaatatctg ggcgcagtaa aatcaagcgg tgccttggaa atttccacat tggtcgatgt ggaaaaaaag ggcggcgacg taaaaatatt taaactcaga aactttccgg tggattacta tgtgatcgga gttcatgggc ctgcgtttcg gaagaatacc aattttggaa tctctgtcgt tgaagttttc tcctccttat tttcgatggt tactttggga ttgatcccgg tatattcgtc ggatctcgca aaaacggaag taaaaattta tgataaaaat ctaaaattag taaacagttt agaatatgat aattcctatt ctacgatcga tgcaatatgg gcatcgccga atccgcctca ctgtaaaatg ttagagtgta cggagcaaat tggttctcca cctagtattg tttattccga aatgggtccg agaatcgaag aggacgtttt gaattcgatt caaaaacctg ccgcccctac caattaa
Details for LpboA.19658.a.B2.GE41888
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMH YYVKEFPVSG KAKVEKAPKI AYLGFRTYQS RVTGSASRRT TYTAELVYET RTIPKLENGV FINQLKSSGF RGDIPSDKVQ AFAMEYLGAV KSSGALEIST LVDVEKKGGD VKIFKLRNFP VDYYVIGVHG PAFRKNTNFG ISVVEVFSSL FSMVTLGLIP VYSSDLAKTE VKIYDKNLKL VNSLEYDNSY STIDAIWASP NPPHCKMLEC TEQIGSPPSI VYSEMGPRIE EDVLNSIQKP AAPTN
Validated NT Sequence
atcttactca cttaattggt aggggcggca ggtttttgaa tcgaattcaa aacgtcctct tcgattctcg gacccatttc ggaataaaca atactaggtg gagaaccaat ttgctccgta cactctaaca ttttacagtg aggcggattc ggcgatgccc atattgcatc gatcgtagaa taggaattat catattctaa actgtttact aattttagat ttttatcata aatttttact tccgtttttg cgagatccga cgaatatacc gggatcaatc ccaaagtaac catcgaaaat aaggaggaga aaacttcaac gacagagatt ccaaaattgg tattcttccg aaacgcaggc ccatgaactc cgatcacata gtaatccacc ggaaagtttc tgagtttaaa tatttttacg tcgccgccct ttttttccac atcgaccaat gtggaaattt ccaaggcacc gcttgatttt actgcgccca gatattccat tgcaaaagct tgaaccttat cggaaggaat atcacctcta aacccgctgc ttttcagctg attaataaaa acaccgtttt ccaacttcgg aatggttcta gtttcataga ctaactcggc tgtatacgta gtccttctgc tcgcagaccc ggttactctg gattgatacg ttctaaaacc gagatatgca attttaggag ccttttccac tttagctttc ccggaaacag gaaactcctt cacgtaataa tgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHHY YVKEFPVSGK AKVEKAPKIA YLGFRTYQSR VTGSASRRTT YTAELVYETR TIPKLENGVF INQLKSSGFR GDIPSDKVQA FAMEYLGAVK SSGALEISTL VDVEKKGGDV KIFKLRNFPV DYYVIGVHGP AFRKNTNFGI SVVEVFSSLF SMVTLGLIPV YSSDLAKTEV KIYDKNLKLV NSLEYDNSYS TIDAIWASPN PPHCKMLECT EQIGSPPSIV YSEMGPRIEE DVLNSIQKPA APTN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcattatt acgtgaagga gtttcctgtt tccgggaaag ctaaagtgga aaaggctcct aaaattgcat atctcggttt tagaacgtat caatccagag taaccgggtc tgcgagcaga aggactacgt atacagccga gttagtctat gaaactagaa ccattccgaa gttggaaaac ggtgttttta ttaatcagct gaaaagcagc gggtttagag gtgatattcc ttccgataag gttcaagctt ttgcaatgga atatctgggc gcagtaaaat caagcggtgc cttggaaatt tccacattgg tcgatgtgga aaaaaagggc ggcgacgtaa aaatatttaa actcagaaac tttccggtgg attactatgt gatcggagtt catgggcctg cgtttcggaa gaataccaat tttggaatct ctgtcgttga agttttctcc tccttatttt cgatggttac tttgggattg atcccggtat attcgtcgga tctcgcaaaa acggaagtaa aaatttatga taaaaatcta aaattagtaa acagtttaga atatgataat tcctattcta cgatcgatgc aatatgggca tcgccgaatc cgcctcactg taaaatgtta gagtgtacgg agcaaattgg ttctccacct agtattgttt attccgaaat gggtccgaga atcgaagagg acgttttgaa ttcgattcaa aaacctgccg cccctaccaa ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat